Fluigent 2-SWITCH User Manual

Microfluidic valves, easy switch solutions
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USER'S
MANUAL
EASY
SWITCH
SOLUTIONS
MICROFLUIDIC VALVES

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Summary of Contents for Fluigent 2-SWITCH

  • Page 1 USER’S MANUAL EASY SWITCH SOLUTIONS MICROFLUIDIC VALVES...
  • Page 2: Table Of Contents

    PRECAUTIONS SUMMARY INTRODUCTION QUICK START GUIDE Aria is a perfusion system which automates perfusion or timed Do not open SWITCHBOARD, 2-SWITCH , M-SWITCH or L-SWITCH 2 SWITCH VALVE injection protocols. It allows for the sequential delivery of up to devices. Please refer all service issues to our Support department (support@fluigent.com)
  • Page 3: Introduction

    4 L-SWITCH and up to 8 2-SWITCH . The device allows for communication of the SWITCHes to a PC with Fluigent software : OxyGEN for live control or to automate protocols. It also ensures power supply to the connected valves.
  • Page 4: Quick Start Guide

    See page 31 for the different L-SWITCH™ positionning options If using a L-SWITCH , connect the device RJ45 port with the provided flat RJ45 white cable. After the experiment, do not forget to rinse the 2-SWITCH™, Note: If one is using a 2-SWITCH or M-SWITCH , the RJ45 M-SWITCH™...
  • Page 5: Switch Tm Valve

    2-SWITCH™ is to plug one of the two ports (#1 or #2). This way, one of the positions of the 2-SWITCH™) becomes a closed position, and the 2-SWITCH™ acts as an “ON/OFF” The 2-SWITCH™ is a 3-port / 2-position valve. Each switch.
  • Page 6 Port number 1 White LED for position 2 the top face of one 2-SWITCH™ into the bottom face of another one. A magnet holds the two 2-SWITCH™ together. Two LED indicators on the front show the current position of the 2-SWITCH™.
  • Page 7 2-SWITCH™ operation. FLUIGENT advises you tubing and verify that it remains secure. to use only these fittings on the 2-SWITCH™. Please note that only tubing of 1/16’’ external diameter should be used with the 2-SWITCH™. Use of other tubing sizes with or without tubing...
  • Page 8: M Switch Tm Valve

    M SWITCH VALVE There are several ways to position the 2-SWITCH™. The 2-SWITCH™ can be used in any spatial configuration. The M-SWITCH™ is an 11-port / 10-position valve. This means that eleven (11) ports can be connected with tubing, such that it is possible to choose between ten (10) individual positions linking one of the ten (10) external ports to the central port of the valve.
  • Page 9 M SWITCH M SWITCH M-SWITCH™ is a bidirectional valve. The fluid can flow inside the M-SWITCH™ in both directions. This feature plus the rotary Photo of the M-SWITCH™. capabilities allow different ways of use. Distributor mode: fluid flows from the central port to one external port (according to the selected position).
  • Page 10 Thread-size: ¼-28 • Flat-bottom type (FB). • Tubing compatibility: 1/16’’ external diameter (1/16’’ OD). Fluigent provides the following contents: An RJ45 cable connects the M-SWITCH™ to the SWITCHBOARD. Plug the cable into dedicated port on the SWITCHBOARD (white 20 * Flat bottom ferrules 1/16'' cable should be plugged in white port).
  • Page 11 M SWITCH M SWITCH Cut the 1/16’’ OD tubing to the desired The M-SWITCH™is lested in order to have the best stability length, leaving a square-cut face. when placed on a surface. The M-SWITCH™ can be used in standing position or horizontally as long as it is stable. Slide the ¼...
  • Page 12 fluid, corresponding to the sample loop size valve for injection or switching different fluids. The switch position and timing may also be automated using Fluigent OxyGEN software. Load sample position The fluid paths of the L-SWITCH™...
  • Page 13 L SWITCH L SWITCH A RJ45 cable allows for connection between the M-SWITCH™ the SWITCHBOARD. Connect the cable into dedicated port on the Photo of the L-SWITCH™. SWITCHBOARD (white cable should be plugged in white port). A. The orange LED lights up The 6 connection ports are when the valve is powered.
  • Page 14 Tubing compatibility: 1/16’’ external diameter (1/16’’ OD). Insert into a port of L-SWITCH™, twist until it is tightened. You can To get started, Fluigent provides the following contents: pull gently the tubing to verify that it is securely connected to the port.
  • Page 15 L SWITCH L SWITCH A sample loop is used to inject a precise volume of liquid. In order to connect a sample loop to the L-SWITCH™, one will need two In this example, the fluid circuit enters from the port 2 and exits from nuts and two ferrules, in addition to the the port 3.
  • Page 16 L SWITCH L SWITCH There are several possibilities to set the L-SWITCH™ Syringes are often used to load a sample loop (See the previous The L-SWITCH™ can be used in any spatial conf iguration. example p.32) To do so, one needs to use the adaptor provided to connect a syringe needle to the L-SWITCH™...
  • Page 17: Switchboard

    Photo of the SWITCHBOARD. A second group of eight RJ45 ports (Port 1 to Port 8), called “Blue cables” to connect up to eight (8) 2-SWITCH™ Two light indicators are associated with each RJ45 ports: The orange indicator lights up when an M-SWITCH™, an L-SWITCH™...
  • Page 18 To connect a 2-SWITCH™ to the SWITCHBOARD, plug the end of the on the front of the SWITCHBOARD labeled blue RJ45 cable to one of the eight (8) 2-SWITCH™ RJ45 ports on the “power”. The flat side of the power supply SWITCHBOARD.
  • Page 19: Fluidic Circuit Examples

    Connect the M-SWITCH™ and the L-SWITCH™ to RJ45 to ports located in the ‘White cable’ SWITCHBOARD section (A to D) This type of set-up can be used for applications including: and connect the 2-SWITCH™ RJ45 to ports located in the ‘Blue • Cell analysis cable’...
  • Page 20 Several samples are injected simultaneously or separately within of interest are combined into water in oil droplets. These droplets are a Y-shaped chip by changing the position of the three 2-SWITCH then sorted at the outlet of the chip using the M-SWITCH placed at the chip inlets.
  • Page 21 OxyGEN software allows one to switch the valve position and the sample is then sorted by the 2-SWITCH at the outlet of the chip. pressurized reservoir so that the flow is maintained unidirectionally without being stopped except for the brief period of the switching...
  • Page 22 MANDATORY PRACTICES The following section describes the steps to perform the cleaning of 1. Filter your solutions. Fluigent microfluidic valves (2-SWITCH, L-SWITCH and M-Switch) depending on your application. 2. Rince each port of your valve with your buffer for 1 min 3.
  • Page 23: Frequently Asked Questions

    2-SWITCH™ before screwing the plug? with external diameters different from 1/16’’? If you are planning to use a 2-SWITCH™ as a fluidic ON/OFF switch, you The M-SWITCH™ can only be connected with 1/16’’ OD tubing. The same will need to plug either port #1 or port #2 on the 2-SWITCH™.
  • Page 24: Technical Specifications

    SPECIFICATIONS TECHNICAL SPECIFICATIONS L SWITCH 2 SWITCH Pressure rating Up to 2,5 bar Pressure rating Up to 7 bar (36 psi) (100 psi) Internal volume 28 L Internal volume 660 nL Wetted materials PEEK, PPS, FFKM, Mineral oil is not Wetted materials PEEK FKM, EPDM...
  • Page 25: Servicing & Warranty

    SERVICING & WARRANTY What This Warranty Covers This warranty is granted by Fluigent and applies in all countries. Your Fluigent product is guaranteed for one year from the date of delivery at your lab- oratory against defects in materials and workmanship. Component...
  • Page 26 VERSION JAN. 2025...

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