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APP96
Quick Start Manual
TM
Tray Holder (RT)
LYSE (-BCT)
DIGEST
Tray 1: Elution
STOP
Tray 2: LYSE (-BCT)
1 - 48 samples
1
49 - 96 samples
6
Wash1
Wash2
Tray 3: POPtips
ELUTE
1: Elution (RT): Position for COLLECTION PLATE
2: Positions for Lyse (Lyse-BCT) reagent
3: Position for POPtips
4: Empty position
5: Positions for all other iST or iST-BCT reagents
6: Elution (cooled): Position for COLLECTION PLATE
7: Position for SAMPLE PLATE
8: Container for solid waste
PreOmics GmbH
Martinsried | Germany
Figure 1. APP96 overview.
Tray Cooler (10 °C)
Tray 4: Empty
Tray 5: Other Reagents
1
2
6
7
Tray 6: Elution
Figure 2. Deck layout.
placement
TM
PreOmics Inc
Billerica, MA | USA
Thermal Mixer
Tray 7: Sample Plate
3
4
5
8
9
10
Tray 8: Waste
Container
1: Dilutor
2: Park Station
3: Fast Wash
4: Tray Holder
5: Robotic Arm
6: Tray Cooler
7: Thermal Mixer
8: Waste Container
9: Terminal
Version 1 - For research use only
M-000044
.
www
preomics.com
support@preomics.com
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Summary of Contents for PreOmics APP96
- Page 1 3: Fast Wash 4: Tray Holder 5: Robotic Arm 6: Tray Cooler 7: Thermal Mixer 8: Waste Container 9: Terminal Figure 1. APP96 overview. Tray Holder (RT) Tray Cooler (10 °C) Thermal Mixer LYSE (-BCT) DIGEST Tray 1: Elution Tray 4: Empty...
- Page 2 APP96 Quick Start Manual 1. Power the APP96 on by switching both external power supplies and wait until the self-test has finished, indicated by the green LED steady on. 2. Attach the bottle containing the operating solution (89.9% ddH O, 10% acetonitrile, 0.1% formic acid) to Dilutor Port 2, ensuring that the tubing reaches the bottom of the bottle.
- Page 3 APP96 Quick Start Manual 6. Place the SAMPLE PLATE on the Thermal Mixer and confirm that it is firmly attached. Ensure that the protective layer is removed from the sealing foil, as described in step 4. Tray Holder (RT) Tray Cooler (10 °C)
- Page 4 APP96 Quick Start Manual 10. When the instrument setup is finished, continue with the Terminal. Navigate on the Terminal to “Local Scripts” by pressing the left button. Select “Methods” Choose the desired method and click on “Edit”. Change the number of samples and the start position on the deep-well plate (=Index). Edit further parame- ters if necessary and click on “Save”.
- Page 5 Enter button for confirming a selection Scroll wheel for choosing an option from a menu or changing a value or character Script Defines the steps executed by APP96 (=assay or work- flow) Method Defines the variable parameters in a workflow (e.g., sample number, start index) Figure 1.
- Page 6 APP96 Quick Start Manual Table 1. Hardware & selectable parameters Ensure that the hardware components (fastWashStation, thermalMixer_1, and toolDilutor) are properly set up (this setup is required only the first time using the method). Parameter Default Min-Max numberOfSamples 1–96 startIndex 1–96...
- Page 7 APP96 Quick Start Manual 4. Start a Job a. Go to “Local Scripts” Job Queue Enter b. Select Start or Start Job 1. Selecting Start From Job 1 will execute all existing Jobs, including Job 1 5. Finish a Job Once the Job is complete, this will be shown on the Terminal.
- Page 8 APP96 Quick Start Manual 8. Import a new script If a new script/workflow is offered by PreOmics, the script has to be uploaded on the instrument via the Terminal. a. Insert a USB-Stick (FAT32) with the script (as .xml file) inside the folder “Scripts” in the PAL USB- Port (back side of the XY-axis) b.
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