1. Manuals
  2. Brands
  3. Drawell Manuals
  4. Laboratory Equipment
  5. Gentier 96E
  6. User manual

Drawell 96E User Manual

Real-time pcr system
Hide thumbs Also See for 96E:
Table of Contents

Advertisement

Quick Links

Download this manual
Drawell International Technology Limited
Real-time PCR System
User Manual
Please read operating manual before installation and operation.
Drawell International Technology Limited
Chongqing Drawell Instrument Co., Ltd.
Shanghai Drawell Scientific Instrument Co.,Ltd.
Address : Suite 2705,Building No.12,Shiyou Road No.1, Yuzhong District, Chongqing
China.
Homepage : www.drawell.com.cn
Tel : 0086-023-63268643
Email : sales05@drawell.com.cn

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the 96E and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Drawell 96E

Summary of Contents for Drawell 96E

  • Page 1 Real-time PCR System User Manual Please read operating manual before installation and operation. Drawell International Technology Limited Chongqing Drawell Instrument Co., Ltd. Shanghai Drawell Scientific Instrument Co.,Ltd. Address : Suite 2705,Building No.12,Shiyou Road No.1, Yuzhong District, Chongqing China. Homepage : www.drawell.com.cn Tel : 0086-023-63268643 Email : sales05@drawell.com.cn...

  • Page 2: Intended Use

    Drawell International Technology Limited Intended Use The real-time PCR system is intended for performing rapid, accurate polymerase chain reaction (PCR), meanwhile real-time measuring nucleic acid signals from DNA-binding fluorescent dyes or labeled probes and converts them to comparative quantitative readouts of DNA or reverse transcribed RNA.
  • Page 3 Drawell International Technology Limited Safety Labels on Transport Package Label Description Fragile: the items inside are fragile, please handle with care. This Side Up: indicates the upward side of the transport package. Keep Dry: keep the transport package away from rain or any liquid.
  • Page 4 Drawell International Technology Limited Warning danger: indicates that a certain part of the real-time PCR system may cause personal injury. IVD Equipment: the real-time PCR system belongs to In Vitro Diagnostic equipment. European Community Representative: indicates the authorized representative of this instrument in European.

  • Page 5: Symbols Used In This Manual

    Drawell International Technology Limited Symbols Used in This Manual Symbol Description Caution: reminding user to pay attention to a certain operation. Operating the instrument in any manner unspecified in this manual may results in instrument damage or abnormal function. Reminding: providing important information regarding the Real-time PCR system operation and successful application, including the information explained in further detail elsewhere in this manual.

  • Page 6: Safety And Regulatory Compliance

    Drawell International Technology Limited Safety and Regulatory Compliance The operation, maintenance and repair of real-time PCR system shall strictly follow the safety specifications listed in this section and through this manual. The design of Real-time PCR system has fully considered its biological contamination protection, electrical safety protection and mechanical motion protection.
  • Page 7 Drawell International Technology Limited dust cover and other materials while it is running. Caution: the installation and transportation of the real-time PCR system should be performed by professional engineer or under professional guidelines. Caution: please remove the transport lock after instrument installation and reserve the transport lock for future use.
  • Page 8 Drawell International Technology Limited any part of the instrument while it is electrified. Caution: the real-time PCR system should be properly grounded, any damage of the internal or external grounding path may cause dangerous. Caution: in case of electric leakage, immediately unplug the instrument and stop using.
  • Page 9 Drawell International Technology Limited Prohibit: never run the real-time PCR system in places that have or may have flammable and explosive gas. Prohibit: spraying liquid on electrical parts may cause a short circuit and result in fire, do not use sprays in vicinity of the real-time PCR system.

  • Page 10: Table Of Contents

    Drawell International Technology Limited Content A. Overview ............................1 1. A ..............................1 PPLICATION IELDS 2. P ...................1 RODUCT ONSTITUTE AND NSTRUMENT TRUCTURE 2.1 Product Constitute..............................1 3. P .............................. 2 2.2 Instrument Structure..............................1 RODUCT NTRODUCTION 3.1.1 Instrument General Parameters......................2 3.1 Instrument Parameters and Characters......................2...
  • Page 11 Drawell International Technology Limited D. Application Software Operation ..................20 1. S .......................... 20 TARTUP PPLICATION OFTWARE 2. Q ................................21 UICK TART 3. M ................................24 NTERFACE 3.1 Menu Bar..................................25 3.2 Tool Bar..................................43 3.3.1 Run Setting..............................46 3.3 Operation Area................................44 3.3.2 Sample Setting............................
  • Page 12 Drawell International Technology Limited 1.2 Clean Touch Screen...............................130 2. I .................131 1.3 Clean Loading Platform and Sample Block....................130 NSTRUMENT AINTENANCE PERATION NSTRUCTIONS 2.1 Maintain Sufficient Air Flow..........................131 2.2 Maintain Stable Power Supply........................131 2.3 Maintain Instrument Cleanliness........................131 2.4 Replace Fuse Tube..............................132 3.

  • Page 13: Overview

    Drawell International Technology Limited A. Overview 1. Application Fields The real-time PCR system is intended for performing rapid, accurate polymerase chain reaction (PCR), meanwhile real-time measuring the nucleic acid signals from DNA-binding fluorescent dyes or labeled probes and converts them to comparative quantitative readouts of DNA or reverse transcribed RNA.

  • Page 14: Roduct Ntroduction

    Drawell International Technology Limited Figure A-1. Instrument Structure 1. Touch screen 2. Sample block 3. Loading platform 4&5. Cooling fan 6. Air vent 7. Power switch 8. Fuse tube box 9. Power socket 10&12. USB interface 11. Internet access 13. Removable air vent cover 3.
  • Page 15 Drawell International Technology Limited ▶ Dimension: 670mm(L) × 580mm (W) × 580mm (H)； Package Specification: Weight: 38kg； ▶ Voltage: AC 100~240V； Power Specification: Frequency: 50/60Hz； Rated power: 900VA； Overvoltage category: II； ▶ less than 1.5 meters； Power Cord: ▶ Cat 5e FTP cable with length less than 3 meters；...

  • Page 16: Instrument Technical Parameters

    Reminding: 96R instrument model possess 4 channels. Reminding: the channel 6 of 96E instrument model is for FRET probe, which takes the report group of channel 1 as the energy donor and the report group of channel 3 as the energy receptor.
  • Page 17 Drawell International Technology Limited ▶ Repeatability: CV ≤ 0.5%. ▶ Linear Correlation: │r│≥ 0.999, within the scope of no less than five magnitudes concentration gradients. Man-machine Interactive System Parameters ▶ Touch Screen: with built-in 10.4’ inches color touch screen, providing more simple operation.

  • Page 18: Instrument Characters

    Drawell International Technology Limited Caution: the main control computer of the real-time PCR system is not designed for online use, connect it to internet may cause risks of computer virus infection or hacker attacks. Caution: it is not recommended to install other software on the main control computer of the instrument.

  • Page 19: Software Characters

    Drawell International Technology Limited 3.2 Software Characters ▶ Software Interface: wizard-style interface, intuitive layout and program setting, very easy for operation. ▶ Software Language: with switchable multiple languages. Default languages are Chinese and English. ▶ Multiple Functions: the software possesses multiple analysis functions that can adapt to a variety of experimental requirements.
  • Page 20 Drawell International Technology Limited ▶ 0.2 ml 96-well no skirt PCR plate (clear, white or frosted).

  • Page 21: Instrument Installation

    Drawell International Technology Limited B. Instrument Installation 1. Unpacking Instructions The transport package of the real-time PCR system is as shown in figure B-1. The instrument and its accessories are well preserved in a carton case. In order to prevent the collision and oscillation during transportation, the instrument is sealed with plastic bag and well supported by protective foams.
  • Page 22 Drawell International Technology Limited Figure B-2. Unpacking Steps Reminding: in order to prevent the formation of condensation water, please do not open the transport package until it reaches the room temperature. Reminding: please check the package integrity before open it. In case there is any damage, collision or water mark, please contact the transport department or our company.

  • Page 23: Orking Nvironmental Equirements

    Drawell International Technology Limited Reminding: please fill in the relevant content on installation information feedback table after acceptance, and send it back to our company for document and warranty. Caution: please reserve the original packing materials for future use. The transport package of the real-time PCR system is designed to reduce the instrument damage and ensure its transportation safety.

  • Page 24: Instrument Power Requirements

    Drawell International Technology Limited 2.2 Instrument Power Requirements The power grid environment of the real-time PCR system should possess ground wire and the instrument should be properly grounded. Please ensure the power strip of the real-time PCR system has 3~4 three-phase plugs in order to meet the demands for the instrument, control computer and printer.
  • Page 25 Drawell International Technology Limited Step: there is a removable air vent cover located on the right side of the instrument shell, as shown in figure B-3a. Please use appropriate tool to pry off and remove the removable air vent cover, as shown in figure B-3b.

  • Page 26: External Device Connection

    Drawell International Technology Limited Caution: unless it is noted in this manual, please do not loosen or remove any internal and external screws or other parts, otherwise it will break the instrument warranty and result in instrument misalignment. Caution: the real-time PCR system should be installed by professional engineer or under the guidance of professional engineer.

  • Page 27: Unlock The Real-Time Pcr System

    Drawell International Technology Limited 3.4 Unlock the Real-time PCR system At the first-time real-time PCR system startup. the instrument system software will prompt user to unlock it and the specific operation steps are as follows: step: the instrument system software will prompt user to confirm that the transport lock has been removed, as shown in figure B-4.
  • Page 28 Drawell International Technology Limited Figure B-5. Instrument system software - Unlock the real-time PCR system (2) Figure B-6. Instrument system software - Unlock the real-time PCR system (3)

  • Page 29: Computer Network Setting

    Drawell International Technology Limited 3.5 Computer Network Setting The network address of the control computer should be set according to the default network information of the real-time PCR system. The control computer can connect the identifiable instruments within LAN. step: please first check the network information of the real-time PCR system, which is displayed on the network information window of the instrument software, as shown in figure B-7a.
  • Page 30 Drawell International Technology Limited Figure B-7b. Control computer network setting...

  • Page 31: The Preparation Before Experiment

    Drawell International Technology Limited C. The Preparation before Experiment 1. Instrument Self Inspection The real-time PCR system possesses self-inspection function, it is recommended conduct the self-inspection before running any experiment, to ensure that it can work normally. Step: switch on the power switch of the real-time PCR system.

  • Page 32: Application Software Operation

    96E instrument model of real-time PCR system. For different instrument models, the software interface will display the corresponding features and instrument model names, this user manual will specify the differences of software interfaces or functions.

  • Page 33: Uick Stbart Bar

    Drawell International Technology Limited Figure D-1. Startup interface of application software 2. Quick Start Bar The quick start bar of application software is as shown in figure D-1.1. At this interface, user can login, create new experiments, open data file, set default instrument, view instrument information and conduct other operations.
  • Page 34 Drawell International Technology Limited name in the User Name input box and click < OK > to register a new user account. Figure D-1.1b Quick start bar - Add User ▶ Quick Start tab: includes four shortcut keys: < New Experiment >, < New Experiment from Test Template >, <...
  • Page 35 Drawell International Technology Limited will create a new experiment file with same experiment settings as the selected test template. Figure D-1.1d Quick start bar - New Experiment from Test Template  Click < New Experiment from Existing Experiment > and the application software will automatically pop up the choose experiment file window;...

  • Page 36: Main Interface

    Drawell International Technology Limited bar, including IP Address, On-line, Loading Platform and Status information; when user open the application software again after setting the default instrument, the application software will automatically connect the default instrument and display its status. ▶ User can check the Display at Startup check box to decide whether to open the quick start bar automatically when starting the application software.

  • Page 37: Menu Bar

    Drawell International Technology Limited 3.1 Menu Bar The menu bar of application software includes: File (F), View (V), Tool (T), Option (O) and Help (H) five submenus. File (F) submenu: Options and Functional Description 1. New Experiment (N): create a new experiment.
  • Page 38 Drawell International Technology Limited 1. Quick Start: open the Quick Start bar. For detailed functions, refer to D-2 Quick Start Bar. 2. Show Toolbar: user can decide whether to Show Toolbar on the main interface of application software. For detailed functions, refer to D-3.
  • Page 39 Drawell International Technology Limited Figure D-2.2 Tool submenu - Instrument Management interface ▶ Instrument List: displays relevant information of instrument connected, including Model, Name, Serial No., Remarks, IP Address and whether the instrument is set as the Default Instrument. There are four function keys under the list: < Add >, < Edit >, < Delete > and <...
  • Page 40 Drawell International Technology Limited  User can click < Scan > and the application software will automatically scan all identifiable instrument within LAN and display their IP Address and Model in Instrument List.  User can double click a certain instrument IP address in the Instrument List and the selected IP address will be displayed in the IP Address input box on the right;...
  • Page 41 Drawell International Technology Limited a. Information Areas:  Instrument Information Area: display the relevant information of instrument selected in the Instrument List, such as Name, IP Address, Model, Serial No. and Version No. of instrument components, etc.  Computer information Area: display the IP address list of the Connected Computer within LAN.
  • Page 42 Drawell International Technology Limited c. Instrument File: display all experiment files and data files saved in the connected instrument.  Click < → > to download the selected Local File to the connected instrument.  Click < ← > to upload the selected Instrument File to the control computer and save in the Local Directory selected by user.
  • Page 43 Drawell International Technology Limited 3. Research Report: Click Research Report option in Tool submenu and the application software will automatically pop up the research report interface, as shown in figure D-2.4. Reminding: user shall create an experiment before entering the research report interface.
  • Page 44 Drawell International Technology Limited list after the icon to save the research report as a PDF or html file. ▶ Print Research Report: After editing the research report, user could click < icon to choose a printer and select Print option from the drop-down list after the icon to Print >...
  • Page 45 Drawell International Technology Limited the check box to determine whether to apply the lid heating function; e. Test Target :  Number of Well(s) for one sample: user can manually input or use keys to set the number well(s) for one sample, and select well arrangement orientation from the subsequent drop-down menu: Horizontal or Vertical.
  • Page 46 Drawell International Technology Limited Figure D-2.5b Research Report interface – Program ▶ Analysis Parameters tab: is as shown in figure D-2.5c. Figure D-2.5c Research Report interface – Analysis Parameters...
  • Page 47 Drawell International Technology Limited a. Analytical Method : user can set the corresponding analysis parameters for the current template according to the experiment type and data analysis requirement. b. Select Stage : user can choose the stages that need to be analyzed.
  • Page 48 Drawell International Technology Limited Figure D-2.6 Test Template Management Option (O) submenu: includes following options. Options and Functional Description 1. Configuration Management: Click Configuration Management option in the Option submenu and the application software will automatically pop up the configuration...
  • Page 49 Drawell International Technology Limited Figure D-2.7a Option submenu - Configuration Management interface - Default Path tab Interface Introduction and Functional Description ▶ Default Path tab: the configuration management interface displays the Default Path tab by default, as shown in figure D-2.7a.
  • Page 50 Drawell International Technology Limited application software for temperature program stage settings. Figure D-2.7b Configuration Management interface - Standard Temperature Template tab  User can modify the predefined Standard Temperature Template in this tab, change the corresponding stage and step settings. For detailed information, please refer to D-3.3...
  • Page 51 Drawell International Technology Limited setting for that gene.  User can click < Save > to save the analysis parameter setting for the current experiment.  User can click < Cancel > to cancel the analysis parameter setting for the current experiment.
  • Page 52 Drawell International Technology Limited Figure D-2.8b Parameter Management interface - HRM Curve tab ▶ Genotyping tab: User can manage the analysis parameters of specific or multiple genes for genotyping experimental results analysis on the Genotyping tab, as shown in figure D-2.8c.
  • Page 53 Drawell International Technology Limited Figure D-2.8c Parameter Management interface – Genotyping Curve tab 3. User: click User option in the Option submenu and the application software will automatically pop up the user interface, as shown in figure D-2.9a. Figure D-2.9a User interface - User Management tab ▶...
  • Page 54 Drawell International Technology Limited  < Delete >: admin user can delete general user accounts in User Management tab. ▶ General User Permissions tab: as shown in figure D-2.9b. Figure D-2.9b User interface - General User Permissions tab a. If user login as admin user account, user can manage general user permissions.

  • Page 55: Tool Bar

    Drawell International Technology Limited Figure D-2.10 Help submenu - About interface 3.2 Tool Bar The Tool Bar of the application software consists of eight commonly used function icons. Options and Functional Description New Experiment >: create a new empty experiment.

  • Page 56: Operation Area

    Drawell International Technology Limited 3.3 Operation Area After creating a new experiment file or open an experiment file, the operation area on the main interface of the application software will be activated, as shown in figure D-3. Figure D-3. Operation area on main interface The experiment file created or opened will be displayed in the experiment file tab at the bottom of operation area and user can select or close any experiment file in this tab.
  • Page 57 Drawell International Technology Limited 3. When the application software is in the interface of Run Setting, Sample Setting, Run Monitoring or Analysis tab, different function icons will be shown accordingly in the tool bar of application software. ▶ Run Setting tab: when the Run Setting tab is selected on the main interface, two additional function icons will be shown in the tool bar on the run setting interface.

  • Page 58: Run Setting

    Drawell International Technology Limited 3.3.1 Run Setting The operation area on main interface displays Run Setting tab by default, as shown in figure D-4. Figure D-4. Run Setting interface Run Setting The Run Setting interface consists five parts: temperature program area, stage and step lists, experiment and step editing areas.
  • Page 59 Drawell International Technology Limited Figure D-4.1 Temperature program area – Example temperature program Introduction to Temperature Program Area  Stages are longitudinal separated; if a stage contains several steps, these steps are separated by solid blue line;  The corresponding stage type and cycle number are displayed below each stage box and the corresponding step number is displayed above the step box;...
  • Page 60 Drawell International Technology Limited Figure D-4.2 Experiment editing area ▶ Tube Type: user can choose the consumable type of current experiment from drop-down list of Tube Type, including Clear, White and Frosted. ▶ Reaction Volume: user can enter the reaction volume for current experiment in the Reaction Volume input box.
  • Page 61 Drawell International Technology Limited Reminding: user can also double click the cycle number below the stage box in the temperature program area to complete the cycle setting. Reminding: the cycle number setting range is 1-99. User can add or delete any stage according to the experiment requirements or adjust the sequence of each stage.
  • Page 62 Drawell International Technology Limited Introduction to Stage Type Window The application software provides the following stage types: Preincubation, Reverse ▶ and Cooling. User can choose the corresponding predefined stage in the stage type window Transcription, 2 Step Amplification, 3 Step Amplification, Melting, Continuous Melting and click <...
  • Page 63 Drawell International Technology Limited  User can click < Down > icon to move down the selected step. Reminding: user can also click < Insert Step > or < Delete Step > above any step box in the temperature program area to add or delete a temperature step.
  • Page 64 Drawell International Technology Limited keys, or manually enter the current step temperature in the Temperature input box. Reminding: the temperature setting range is 0.0°C -100.0°C. Time: the time for which the temperature is to be held. User can enter the current step time in the Time input box.
  • Page 65 Drawell International Technology Limited Reminding: the start cycle setting range is 1-max cycle number of the current stage. Reminding: the Delta Temperature setting range is 0.1°C- 5.0°C. III. Long Step Mode: the long step mode allows the temperature program to change the elongation step temperature holding time from the initial time to the target time as the cycling proceeds.
  • Page 66 Drawell International Technology Limited Figure D-4.6d. Step Setting Dialog Box – Gradient Step Mode Reminding: the temperature center setting range is 35.5°C - 99.5°C. Reminding: the temperature offset setting range is 0.5°C - 20.0°C. ▶ Details: User can click < Details > to the specific gradient temperatures of each column and the Gradient distribution, as shown in figure D-4.6e.
  • Page 67 Drawell International Technology Limited the melting stage. If the current step is the last step of melting stage, user can set the temperature Increment in the Step Setting dialog box, as shown in figure D-4.6f. Figure D-4.6f. Step Setting Dialog Box – Melting Step ▶...

  • Page 68: Sample Setting

    Drawell International Technology Limited 3.3.2 Sample Setting After finishing the experiment run setting, user can click Sample Setting tab to enter the sample setting interface, which is consisted of sample setting area and sample property editing area, as shown in figure D-5.
  • Page 69 Drawell International Technology Limited Figure D-5.1. Sample Setting interface - Sample Setting area Introduction to Sample Setting Area User can select the relevant sample well in the sample setting area and operations as follows: ▶  Click and select a single well in sample setting area to.
  • Page 70 Drawell International Technology Limited  Click < All > on the top left corner of sample setting area to select all sample wells. 2. Sample Property Setting Area: after selecting one or more sample wells in the sample setting area, user can set the properties for the corresponding wells in the sample property setting area.
  • Page 71 Dye: user can check the relevant dye check box to determine the detection dyes for the selected wells. Reminding: figure D-5.2 takes 96E instrument models of real-time PCR system as an example, the application software displays the corresponding dye of six channels.
  • Page 72 Drawell International Technology Limited select corresponding standard sample wells in the sample setting area and let the application software to automatically calculate and set the relevant standard concentration for this series of standard samples. Please click < Auto Setting > and the auto concentration setting window will pop up automatically, as shown in figure D-5.4.
  • Page 73 Drawell International Technology Limited samples.  User can click < Cancel > and the application software will give up the current auto concentration setting and return back to the sample setting interface. ▶ Replicate: according to experiment requirements, user can classify the same samples into one replicate group in the Replicate group setting box, as shown in figure D-5.5.
  • Page 74 Drawell International Technology Limited  User can select the starting value for replicate group number from the Starting Replicate drop-down list;  User can select the direction for auto setting from Direction drop-down list: Horizontal (from Left to right) and Vertical (from up to down);...

  • Page 75: Run Monitoring

    Drawell International Technology Limited ▶ QC Failure Condition Setting: after completing the sample well setting, please click icon and the QC failure condition setting dialog box will pop up automatically, as shown in figure D-5.7. Figure D-5.7. Sample Setting interface - QC Failure Condition Setting ...
  • Page 76 Drawell International Technology Limited Figure D-6. Run Monitoring interface Interface Introduction and Parameter Description The run monitoring interface consists of six functional modules: Real-time Fluorescence, Temperature Program, Sample Setting, Run Info, Sample Info and Heat Map; after entering the run monitoring interface. This interface is divided into four areas and the application software displays four functional modules (Real-time Fluorescence, Temperature Program, Sample Setting, Run Info) by default.
  • Page 77 Drawell International Technology Limited cycle number of the current running experiment, as shown in figure D-6.1. Figure D-6.1. Run Monitoring interface - Real-time Fluorescence function module ▶ User can set fluorescence curve display mode from the drop-down list on the bottom left of Real-time Fluorescence functional module.
  • Page 78 Drawell International Technology Limited Export >: user can click < Export > icon to export the current real-time fluorescence monitoring diagram. ▶ < 2. Temperature Program: display the temperature program of the current running experiment, as shown in figure D-6.2.
  • Page 79 Drawell International Technology Limited - Sample Setting area. ▶ Function key introduction: user can select one or more sample wells in the sample setting function module to display the corresponding fluorescence curve and relevant data in the Real-time Fluorescence and Sample Info function modules.

  • Page 80: Analysis

    Drawell International Technology Limited Figure D-6.5. Run Monitoring interface - Sample Info function module 6. Heat Map: display the real-time fluorescence heat map of the current running experiment, as shown in figure D-6.6. Figure D-6.6. Run Monitoring interface - Heat Map function module 3.3.4 Analysis...

  • Page 81: Abs Quant

    Drawell International Technology Limited Figure D-7. New Analysis window ▶ The application software provides user with six analysis methods: Abs Quant, Rel Quant, Melting Curve, High Resolution Melting, Genotyping and End Point Fluorescence.  Select Stage: user can choose the stages that need to be analyzed.
  • Page 82 Drawell International Technology Limited modules (Amplification Curve, Standard Curve, Sample Setting, Result Table) by default, as shown in figure D-8. Figure D-8. Absolute quantification analysis interface Reminding: user can select the functional module displayed on the analysis interface from the drop-down list on the top right corner of each area.
  • Page 83 Drawell International Technology Limited Figure D-8.1. Abs Quant analysis interface - Amplification Curve functional module ▶ Parameter introduction:  X axis represents the cycle number of the current experiment;  Y axis represents the Relative Fluorescence Unit (RFU); ▶ Function key introduction: ...
  • Page 84 Drawell International Technology Limited Figure D- 8.1a. Abs Quant analysis interface - Coordinate Range Setting Export >: user can click < Export > icon to export the current amplification ▶ < curve diagram. Log View >: user can click <...
  • Page 85 Drawell International Technology Limited ▶ Parameter introduction:  X axis represents the log concentration of samples;  Y axis represents the threshold Ct value;  Dye: display the relevant dye of standard curve;  Target Gene: display the target gene of standard curve;...
  • Page 86 Drawell International Technology Limited file, then import it to the standard curve list. The corresponding standard curve diagram will be displayed in the Standard Curve Preview area below. b. Select Standard Curve: user can call one or more standard curve from the selected standard curve file according to the current experiment requirements.
  • Page 87 Drawell International Technology Limited Figure D-8.3. Abs Quant analysis interface - Sample Setting functional module IV. Result Table: display the sample details and result data of the current experiment. The result table functional module consists of Result and Statistics sub-tabs.
  • Page 88 Drawell International Technology Limited Figure D-8.4b. Result Table functional module– Statistics sub-tab Table Management > and the table management : click window will automatically pop up, as shown in figure 8.4c. User can decide to Display or  < Table Management >...
  • Page 89 VI. Raw Fluorescence: display the raw fluorescence data map of the current experiment, as shown in figure D-8.6. Figure D-8.6. Abs Quant analysis interface - Raw Fluorescence functional module ▶ Parameter introduction:  X axis shows channel 1-6 (96E&96T) or channel 1-4 (96R).  Y axis shows the relative fluorescence unit (RFU).
  • Page 90 Drawell International Technology Limited  Different colors represent A-H rows of sample wells. ▶ Function key introduction:  User can slide the Cycle slider at the bottom of raw fluorescence functional module to view the raw fluorescence value of different wells at different cycles.
  • Page 91 Drawell International Technology Limited experimental data analysis method in the analysis setting window. Amplification Plot tab: user can set the Analysis Mode and Analysis Method of the current experiment within the Amplification Plot tab, as shown in figure D-9.1. Figure D-9.1. Abs Quant Analysis Setting interface - Amplification Plot tab ▶...
  • Page 92 Drawell International Technology Limited automatic baseline setting in the columns of the Baseline list.  User can also click the Manual Baseline option, select one well in the Baseline list, use keys to manually set the Start Cycle and End Cycle of the baseline setting for this sample;...

  • Page 93: Rel Quant

    Drawell International Technology Limited experiment data of the selected sample name. b. Click < Cancel > to cancel operation and return to the analysis interface. Figure D-9.2. Abs Quant Analysis Setting interface – Gene and Sample tab 3.3.4.2 Rel Quant The relative quantification experiment aims to compare the expression level of two or more genes within a same individual.
  • Page 94 Drawell International Technology Limited Figure D-10. Relative quantification analysis interface Interface Introduction and Parameter Description Reminding: the parameter descriptions of Amplification Curve, Sample Setting, Result Table, Standard Curve, Raw Curve, Raw Fluorescence and Heat Map functional modules are same with those introduced in Bar Chart: display the relative ratio relationship between target gene &...
  • Page 95 Drawell International Technology Limited ▶ Parameter introduction:  X axis shows the gene and sample for comparison;  Y axis shows the Relative Quantity ratio of the compared gene and sample; Reminding: the application software set the relative quantity ratio of the reference sample to 1 by default.
  • Page 96 Drawell International Technology Limited c. SD: user can check Show SD check box to display the SD value on Bar Chart functional module. d. Sort: User can use keys to set the display sort order of Bar Chart functional module, including Sample, Dye or Gene.

  • Page 97: Melting Curve

    Drawell International Technology Limited ▶ Dye and Gene: display the name of target gene and its marking dye. a. User can check a certain check box in Ref Gene column and click < OK > to set the reference gene of the current experiment.
  • Page 98 Drawell International Technology Limited Figure D-12. Melting Curve analysis interface Interface Introduction and Parameter Description Melting Curve: display the fluorescence intensity against the temperature, as shown in figure D-12.1. Figure D-12.1. Melting Curve analysis interface - Melting Curve functional module...
  • Page 99 Drawell International Technology Limited ▶  X axis represents the temperature value; Parameter introduction:  Y axis represents the Relative Fluorescence Unit (RFU); ▶  User can select the melting curve display mode from the drop-down list on the bottom left Function key introduction: of Melting Curve functional module.
  • Page 100 Drawell International Technology Limited corresponding temperature; ▶ Function key introduction:  User can select the melting peak display mode from the drop-down list on the bottom left of Melting Peaks functional module. The melting curve can be displayed according to Well Color, Dye Color, Sample Color and Gene Color.

  • Page 101: High Resolution Melting

    Drawell International Technology Limited ▶ Peak Type: user can check the check box of Positive Peak or Negative Peak to determine the type of melting peak to be analyzed. ▶ Peak Area: display the melting peak area appearing with temperature rise.
  • Page 102 Drawell International Technology Limited Figure D-13. High Resolution Melting analysis interface Interface Introduction and Parameter Description Melting Curve: display the fluorescence intensity against the temperature, as shown in figure D-13.1. Figure D-13.1. HRM analysis interface - Melting Curve functional module...
  • Page 103 Drawell International Technology Limited ▶ Parameter introduction:  X axis represents the temperature;  Y axis represents the Relative Fluorescence Unit (RFU);  By default, the application software will automatically determine normalized area of melting curve. The two vertical sliders on the left are the Pre-melting Range threshold lines, which are used to specify the pre-melting temperature range;...
  • Page 104 Drawell International Technology Limited III. Difference Plot: display the curve after subtracting the baseline group curve, after normalization, and optionally, after temperature shift. The appearance of the curve on Difference Plot functional module depends on the selected baseline group. For more details, please refer to D-3.3.4.4 High Resolution Melting - High Resolution Melting Analysis...
  • Page 105 Drawell International Technology Limited High Resolution Melting Analysis Setting User can click the < Analysis Setting > icon in Tool Bar and the application software will automatically pop up the high resolution melting analysis setting window, which consists of Dye and Gene, Sample and High Resolution Melting three tabs.

  • Page 106: Genotyping

    Drawell International Technology Limited range of the normalization area of the melting curve. ▶ Sensitivity: to set the discrimination sensitivity of melting curve. Reminding: by default, the application software will automatically set the corresponding parameters for the fluorescence normalization of melting curve; user can also drag the relevant threshold lines (vertical sliders) on the Melting Curve functioncal module to determine the normalized area of melting curve.
  • Page 107 Drawell International Technology Limited the application software will automatically detect the end point fluorescence and distinguish different genotypes based on the distribution of two dyes on the scatter plot. The Genotyping analysis interface consists of seven functional modules: Amplification Curve, Scatter Plot, Sample Setting, Result Table, Raw Curve, Raw Fluorescence and Heat Map.
  • Page 108 Drawell International Technology Limited Figure D-15.1. Genotyping analysis interface - Scatter Plot functional module ▶ Parameter introduction:  X axis and Y axis represent the Relative Fluorescence Unit (RFU) of different dyes respectively;  Each point represents a sample; different icons represent different genotypes or interpretation results.
  • Page 109 Drawell International Technology Limited Figure D-15.2. Genotyping analysis interface - Result Table functional module  User can double click the title of each column to sort all sample results according to the content in this column.  User can click the title of each column and drag the column to the left or right to adjust the sequence of sample results.

  • Page 110: End Point Fluorescence

    Drawell International Technology Limited ▶ Analysis Mode: same with the introduction of D-3.3.4.1 Abs Quant - Abs Quant Analysis Setting - Amplification Curve tab. ▶ Gene Group Parameter: a. Gene: display the detective dyes of different genotypes; b. Neg Threshold: user can use keys to set the corresponding negative threshold for the X axis and Y axis of scatter plot.
  • Page 111 Drawell International Technology Limited Figure D-17. End Point Fluorescence analysis interface Interface Introduction and Parameter Description End Point Fluorescence Scatter Plot: display the scatter plot of fluorescence intensities measured at end the point of amplification plateau phase, as shown in figure D-17.1.
  • Page 112 Drawell International Technology Limited ▶ Parameter introduction:  X axis represents the sample well index of the current experiment samples;  Y axis represents the End Point Fluorescence (EPF) intensity;  Each point represents a sample; different icons represent the negative or positive interpretation results.
  • Page 113 Drawell International Technology Limited  Analysis Mode: same with the introduction of D-3.3.4.5 Genotyping - Genotyping Analysis  Analytical Method: same with the introduction of Setting - Genotyping tab. D-3.3.4.5 Genotyping - Genotyping Analysis Setting - Genotyping tab. II. Gene and Sample tab: the introduction of relevant parameters and function keys is same with those introduced in D-3.3.4.1 Abs Quant - Abs Quant Analysis Setting.

  • Page 114: Instrument Software Operation

    Drawell International Technology Limited E. Instrument Software Operation The real-time PCR system can run independently from the control computer. User can use the touch screen or external mouse to operate the instrument system software, so as to realize the experiment program setting and other relevant operations.
  • Page 115 Drawell International Technology Limited  Initializing: the instrument system is in the process of initialization;  Ready: the instrument system initialization is completed, ready for running;  Running: the experiment is running;  Pause: the current running experiment is paused;...

  • Page 116: Software Operation Area

    Drawell International Technology Limited Stop Experiment >: stop the current running experiment. This function key is activated when the instrument system is under Running status. ▶ < Open / Close >: open or close the loading platform. ▶ < Reminding: user can also gently push the loading platform to close it.
  • Page 117 Drawell International Technology Limited Figure E-2. Instrument software – Experiment File interface Interface Introduction & Parameter Descriptions Experiment File Displaying Area: display the pre-existed experiment files or file folders within the instrument system, as shown in figure E-2.1. Figure E-2.1. Experiment File tab - Experiment file display area...
  • Page 118 Drawell International Technology Limited User can press the icon of a certain experiment file or file folder in the experiment file ▶ display area to open it. User can slide the slider or press icons to view all the experiment files or file ▶...

  • Page 119: Run Setting

    Drawell International Technology Limited Reminding: the instrument software will name the new experiment file or file folder with creation date and time by default. Figure E-2.4. Experiment File tab - keyboard ▶ < Rename >: rename the selected experiment file or file folder, this key is inactivated when the instrument system is under Running status.

  • Page 120: Temperature Setting

    Drawell International Technology Limited Reminding: the system will automatically enter the run setting interface after new experiment file creation; Reminding: user could also select an experiment file, press Run Setting tab to enter the run setting interface to view or edit the experimental settings.
  • Page 121 Drawell International Technology Limited Hot Lid: user could press the check box of Hot Lid to decide whether to utilize the hot lid heating function, and press icons to edit the hot lid temperature. Reminding: the hot lid temperature setting range is 40°C ~110°C.
  • Page 122 Drawell International Technology Limited Temperature Program Setting Operation Step 1: Add stage User can press < Add > in the stage setting box and the instrument software will automatically pop up a stage type selection window, as shown in figure E-3.2.
  • Page 123 Drawell International Technology Limited Figure E-3.3. Cycle edit box User could set the cycle number in the cycle edit box, press < OK > to confirm the cycle ▶ setting or press < Cancel > to cancel the setting. Reminding: the cycle setting range is 1~99.
  • Page 124 Drawell International Technology Limited ▶ Four Step Mode options are provided by the instrument software: Standard, Touchdown, Gradient and Long. User can select a certain step mode option by pressing the icon in front of it, and the icon will turn blue.
  • Page 125 Drawell International Technology Limited Reminding: the temperature setting range is 0.0°C ~100.0°C. Reminding: the time setting range is 1s~60min. Reminding: the ramp setting range is 0.1°C/s~6.1°C/s. 2) If the Touchdown step mode option is selected, the step edit window will be shown as figure E-3.5.
  • Page 126 Drawell International Technology Limited Figure E-3.5a. Step Edit window - Touchdown step parameter setting  Initial Temp.: the initial value of annealing temperature change range.  Target Temp.: the target value of annealing temperature change range.  Delta Temp.: the temperature change(°C) per cycle.
  • Page 127 Drawell International Technology Limited temperature holding time from the initial time to the target time as the cycling proceeds. User can press the input boxes of Initial Time, Target Time, Delta Time and Start Cycle to edit the corresponding parameters for the current long step, as shown in figure E-3.6a.
  • Page 128 Drawell International Technology Limited E-3.7. Figure E-3.7. Temperature Setting sub-tab - Step Edit window - Gradient step mode ▶ The gradient step mode allows the sample to adopt different temperatures. After setting the temperature center value and the temperature offset value for the current gradient step, the system will automatically calculate the gradient temperatures.
  • Page 129 Drawell International Technology Limited Reminding: the temperature center setting range is 35.5°C ~99.5°C. Reminding: the temperature offset setting range is 0.5°C ~20.0°C. Reminding: the other parameter settings are same with the standard step mode. 5) If the current step is the last step of a melting stage, the step setting window of this step is as shown in figure E-3.8.

  • Page 130: Fluorescence Setting

    Drawell International Technology Limited 6) If the current step is the last step of a continuous melting stage, the step setting window of this step is as shown in figure E-3.9. Figure E-3.9. Step Edit window - Continuous Melting step mode ▶...

  • Page 131: Run Monitoring

     User can check the corresponding check box in the Channel column to set the fluorescence channel for the current experiment ( channel 1-6 96E&96T or channel 1-4 for 96R)  User can press the corresponding icon in the Dye column and select the proper dye from the drop-down list;...
  • Page 132 Drawell International Technology Limited Figure E-5. System software – Run Monitoring interface Run Status Bar: displays the real-time running status of the current experiment, includes Remaining Time, Stage, Cycle and Step. Run Monitoring Option Bar: user can select Fluorescence or Temperature option in the run monitoring option bar to monitor the corresponding content of the current experiment.
  • Page 133 Drawell International Technology Limited The run monitoring diagram will display the real-time Amplification curve of the current experiment by default, as shown in figure E-5.1. Figure E-5.1. Run Monitoring Diagram - Real-time Amplification Curve  X axis represents the cycle number.
  • Page 134 Drawell International Technology Limited Figure E-5.2. Run Monitoring Diagram - Real-time Melting Curve User can press key below the run monitoring diagram to monitor the fluorescence Heat Map of the current experiment, as shown in figure E-5.3. Figure E-5.3. Run Monitoring Diagram - Heat Map ...

  • Page 135: Instrument Sub-Tab

    Drawell International Technology Limited Figure E-5.4. Run Monitoring Diagram - Temperature program 2.4 General Setting The General Setting tab consists of Instrument, Configuration and Service three sub-tabs. 2.4.1 Instrument Sub-tab The General Setting tab displays the Instrument sub-tab by default, as shown in figure E-6.1.
  • Page 136 Drawell International Technology Limited Introduction to Instrument sub-tab ▶ Instrument Serial Number: display the factory serial number of the current real-time PCR system. ▶ Instrument Model: display the current real-time PCR system instrument model. ▶ Instrument Name: display the name of the current real-time PCR system, user can press <...

  • Page 137: Configuration Sub-Tab

    Drawell International Technology Limited 2.4.2 Configuration Sub-tab Figure E- 6.2. General Setting tab - Configuration sub-tab Introduction to Configuration Sub-tab ▶ Network Information: display the network information of the current real-time PCR system. User can press < Edit > and the instrument software will pop up the network information window, as shown in figure E-6.2a.
  • Page 138 Drawell International Technology Limited  In the IP Setting box, user can view all the network IP addresses, or press < Edit > to modify the corresponding parameter.  User can press < Default > to recover the default network IP settings of the current instrument.

  • Page 139: Service Sub-Tab

    Drawell International Technology Limited format.  User can press < Confirm > to confirm the date and time setting;  User can press < Back > to return to the Instrument sub-tab interface. ▶ Language Setting: user could set the instrument system language. The two options provided are English and Simplified Chinese.
  • Page 140 Drawell International Technology Limited Debug Log: user could connect an USB drive to the real-time PCR system and press < Get Debug Log > to download all the log file of the current real-time PCR system, this will help ▶...

  • Page 141: Instrument Cleaning And Maintenance

    Drawell International Technology Limited F. Instrument Cleaning and Maintenance Under proper use condition, the real-time PCR system requires little maintenance. However, the instrument should be cleaned and maintained on a regular schedule for long time and constant use. This section includes the information on cleaning and maintenance of the instrument.

  • Page 142: Clean Touch Screen

    Drawell International Technology Limited electronics or may occur. Caution: please do not use organic or strong detergent to clean the instrument shell, which may ruin the surface coating. 1.2 Clean Touch Screen step: Switch off the instrument and unplug the power cord;...

  • Page 143: Nstrument Maintenance Operation Instructions

    Drawell International Technology Limited 2. Instrument Maintenance Operation Instructions 2.1 Maintain Sufficient Air Flow The placing area of real-time PCR system should be checked regularly, as it requires sufficient air flow to precisely reach the correct target temperature. Please ensure that the air flow is unrestricted and other items around the instrument do not interfering with the air flow.

  • Page 144: Replace Fuse Tube

    Drawell International Technology Limited 2.4 Replace Fuse Tube The fuse tube (type F10AH250V) of real-time PCR system is located in the fuse tube box near the power outlet at rear of the instrument. Before replacing the fuse tube, please switch off the instrument and unplug the power plug, use a screwdriver to pry open the fuse tube box.

  • Page 145: Ransportation Or Return To Factory

    Drawell International Technology Limited 3. Transportation or Return to Factory 3.1 Instrument Disinfection In case the real-time PCR system will be moved to another lab or needed to return to the after-sale service department for maintenance, please first disinfection the instrument and fill in the disinfection certificate.
  • Page 146 Drawell International Technology Limited Step: Press < Next >, the instrument system software will prompt user that the mechanical parts of the real-time PCR system will move and reset, as shown in figure F-5. Figure F-3. Transport locking operation (1)
  • Page 147 Drawell International Technology Limited Figure F-5. Transport locking operation (3) Step: press < lock >, the mechanical parts of the real-time PCR system will move and reset. After this, the instrument will automatically shutdown and prompt user to install the transport lock, as shown in figure F-6.
  • Page 148 Drawell International Technology Limited Step: please use appropriate tool to pry off and remove the removable air vent cover located on the right side of the instrument shell, as shown in figure F-7a. Step: the transport lock screw should be fixed upon two cooling fans, as shown in figure F-7b.

  • Page 149: Product Packaging

    Drawell International Technology Limited 3.3 Product Packaging Please use the original packaging materials to properly pack the real-time PCR system and its accessories in order to prevent the collision and oscillation during transportation. The specific packing steps of the real-time PCR system are shown in figure F-8.

  • Page 150: Roubleshooting

    Drawell International Technology Limited Step: pack the real-time PCR system with plastic bag, as shown in figure F-8a. Step: place the bottom protective foam into the bottom carton, as shown in figure F-8b. Step insert the real-time PCR system into the protective foam, as shown in figure F-8c.
  • Page 151 Drawell International Technology Limited message Ensure there is no other appliance or Power voltage too low circuit in the same electric circuit. Improper operation Restart the instrument system System crashed or out of control Others Contact us Check temperature control...
  • Page 152 Drawell International Technology Limited Drawell International Technology Limited Shanghai Drawell Scientific Instrument Co.,Ltd. Chongqing Drawell Instrument Co.,Ltd. Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District, Chongqing,China Tel: 0086-023-63268643 Web : www.drawell.com.cn Email : sales05@drawell.com.cn...

This manual is also suitable for:

96t96r

Table of Contents