Introduction ............................ 1 Safety precautions .........................3 1 Instrument Introduction ......................9 1.1 Front panel of the instrument ....................9 1.2 Top cover of the instrument ....................10 1.3 Component panel of the instrument ................. 10 1.4 Rear panel of the instrument ..................... 12 2 Instrument Components ......................
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4.1 Pump pressure fluctuation ....................30 4.2 Frequent overpressure ....................... 30 4.3 Large baseline noise ......................31 4.4 Large baseline drift ......................32 4.5 Too high background value ....................32 4.6 Low response value ......................32 4.7 Abnormal suppressor current .................... 33 4.8 No peak ..........................
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B.6 Connecting power cord ...................... 52 B.7 Installation of chromatograph column and suppressor ............. 52 B.7.1 Installation of chromatograph column ............... 52 B.7.2 Installation of suppressor .................... 53 B.8 Connecting waste liquid pipes ................... 53 B.8.1 Installation of waste liquid pipes ................. 54 B.9 Installation of eluent bottle ....................
Safety information This manual contains precautionary instructions and warnings during instrument operation. Please observe them consciously. Security messages are shown in bold and accompanied by icons, as shown below. Caution remind you of the environment that may affect the instrument but will not cause personal injury.
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Electric shock The power supply current used by this instrument is large, which may cause personal injury such as electric shock. Therefore, please turn off the power supply and unplug the power cord before handling. When handling the instrument, please fasten the casing of the instrument. It is forbidden to move the protective casing of live equipment such as circuit boards.
solution which is extremely corrosive. Please do not disassemble the tank by yourself to prevent injury accidents. See section 2.7. Warning Please make sure the system pressure is within the applicable range (≤17MPa) before using the eluent generator to avoid damaging the equipment. section 2.7.
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Caution During manual injection, the valve pulling time should be less than 0.1s, otherwise the pipeline will be broken due to too high pressure on the injection valve. See section 3.6.1. Caution When using aqueous solution as eluent, bacteria are easily generated, thus affecting the experiment.
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instrument, the continuous self-regeneration suppressor uses electrolysis to suppress the background, and a small amount of oxygen and hydrogen will be generated during electrolysis. Gas should be prevented from staying in the waste liquid bottle so as not to generate static electricity. In serious cases, there may be explosion. See section B.8.
1 Instrument Introduction 1.1 Front panel of the instrument The front panel of DW-CIC-D100 Ion Chromatograph is shown in Figure 1-1. Corporate logo Injection valve Figure 1-1 Front panel of the instrument Injection valve The injection valve is a six-way valve made of PEEK material. The sample to be analyzed is manually injected into the sample loop through the injection port by a injector.
1.2 Top cover of the instrument Location of eluent bottle Pipeline passage Figure 1-2 Diagram of the top cover of the instrument 1.3 Component panel of the instrument Conductivity Suppressor detector Column heater Pump display Pump Exhaust valve Figure 1-3 Instrument component Pump display...
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The pump display shows the pressure and flow rate when the system is running.
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The value can be read from the pump display screen. Pump DW-CIC-D100 adopts double-plunger high hydraulic infusion pump. The flow rate range 0.001 mL/min to 9.999 mL/min. However, to achieve optimal performance, the general flow rate is set at 0.200 mL/min to 2.000 mL/min.
The trigger port connects the instrument with the autosampler through a trigger line which triggers the working software to start sampling.If you use ShineLab software, no need to connect this port.When using Drawell software, you need to connect. Data line port The data line port connects the instrument with the computer through the data line, and outputs the signal detected by the ion chromatograph to the working software.
2 Instrument Components 2.1 Pump The Pump equipped for this instrument is an intelligent high-pressure constant-current infusion pump with a built-in micro flow pressure sensor to monitor the flow in real time. it has intelligent pressure limiting function to effectively guard columns. it is matched with the latest pulse decrement technology, providing high stability and low pulsation to meet various applications of ion chromatography.
After the exhaust, tighten the exhaust valve clockwise. 2.2 Injection valve DW-CIC-D100 adopts manual injector and six-way manual injection valve for manual injection. There are two positions in the Injection valve: LOAD and INJECT. During the sample injection at the "LOAD" position, the eluent enters the chromatograph column through the injection valve through the pump instead of through the quantitative loop.
2.4 Conductivity detector The conductivity detector can quantitatively detect the target ions separated by the chromatograph column. DW-CIC-D100 Ion Chromatograph is equipped with constant temperature bipolar conductivity cell. The cell body contains a heat exchanger, and the temperature of the conductivity cell can be adjusted in the range of 5℃ to 60℃. The optimal setting temperature should be 5℃...
:Please turn off the heating button of the conductivity cell and the power switch of the instrument before plugging and unplugging the conductivity cell to avoid the damage to the circuit board. 2.5 Ampere detector (optional) Ampere detectors are commonly used to analyze ions which are difficult to detect with conductivity detectors and have electrical activity and low dissociation degree.
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is shown in Figure 2-2. Figure 2-2 Appearance of DC ampere detector (SHE-3) Ampere detector consists of potentiostat and detection cell. The amperometric cell is a three-electrode volt-amperometric detection cell consisting of a titanium counter electrode, a working electrode and a reference electrode. Thin-layer flow design is adopted so that the smooth electrode surface can reduce flow noise.
electrode surface can be continuously regenerated. Cyclic voltammetric amperometric detection: this method controls the potential to scan repeatedly at different rates and with triangular waveform for one or more times over time, resulting in redox reaction on the surface of the working electrode. 2.6 Suppressor The suppressor can reduce the background conductance of eluent and improve the signal value of ions to be tested, thus improving the testing capability of ion chromatograph.
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principle of electrolyzed water. The device consists of eluent tank, electrolysis generating device, degassing device, circuit control and other parts. The eluent tank contains ultrapure water with resistivity not less than 18.25Ω·cm. When the device is used, only ultrapure water needs to be input at the end of the infusion pump, and no operator is required to prepare a eluent, which can reduce the abrasion of the plunger of the infusion pump by salt solution crystallization and effectively prolong the service life of the infusion pump.
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Figure 2-5 Schematic diagram of eluent generator Flow path connection One end of the eluent generation tank is connected with the ultrapure water output by the infusion pump, and the other end is connected with the ELUENT IN end of the trap column. The two interfaces can be connected at will regardless of sequence.
recommended daily working pressure is >8MP. Precautions Warning the liquid storage tank is filled with high concentration KOH or MSA solution which is extremely corrosive. Please do not disassemble by yourself to prevent injury accidents. Warning please confirm whether the system pressure is within the applicable range (≤17MPa) before using this equipment to avoid damaging this equipment.
The number before the type is the length of the pipeline(For example,150FL=150cm Tetrafluoro Tube 1/8(3.175)*1.6). Pipeline Pipeline Specification Remark Tetrafluoro Tube 1/8(3.175)*1.6 PEEK Tube 1/16*0.13mm peek Tube 1/16*0.25mm peek Tube 1/16*0.50mm peek Tube 1/16*0.75mm Tetrafluoro Tube 1/8*0.5mm Tetrafluoro Tube 1/8*0.75mm Tetrafluoro Tube 1/8*0.80mm It is only used as waste liquid tube, not buffer tube Chromatograph system accessories include the following parts.
Figure 3-1 Software use process 3.1 Start-up Turn on the power supply of DW-CIC-D100 instrument, and the status of each component of the instrument is shown in Table 3-1. Start the software, fill the eluent bottle with eluent, and drain the bubbles in the infusion pump.
The water resistivity should be above 18.25MΩ·cm. DW-CIC-D100 Ion Chromatograph can be equipped with on-line degasser. After providing the degasser, moving phase does not need to be degassed and only needs to be prepared according to chromatograph column requirements.
3.5 Preparation of samples 3.5.1 Selection and preservation of samples Samples are collected in polytetrafluoroethylene bottles cleaned with ultra-pure water. Do not wash the container with strong acid or washing liquid to prevent a large amount of anions from remaining on the container and affecting the accuracy of analysis results. If the sample cannot be analyzed and used on the day of sampling, it should be immediately filtered with 0.22μm filter membrane, otherwise the bacteria in the sample may change the concentration of the sample with time.
The specific method is to add 1 mL of 100-fold concentrated eluent to 100 mL of sample. 3.6 Injection and analysis of samples DW-CIC-D100 supports two injection modes, manual injection and automatic injection. When using an autosampler for injection, disconnect the instrument from its own injection valve line, connect the autosampler injection valve from the pump, and then enter the Guard column.
4. Leave the injector at the injection port end; 5. The software can be started to collect data by quickly turning the injection valve to the INJECT state. Caution The valve pulling time should be less than 0.1s, otherwise the injection valve pressure is too high and the pipeline will easily break.
3.7.2 Weekly check 1. Check whether the instrument pipeline is folded, bent or contaminated. Replace the deformed pipeline in time to avoid affecting the stability of the flow path. If the pipeline is short, reset and replace the pipeline in time. 2.
This chapter lists the causes of some problems that may occur during the operation of DW-CIC-D100 instrument and provides more detailed solutions. When facing similar problems during use, please refer to this section to try to solve them yourself. If you encounter any problem that cannot be solved,Please contact our engineer.
Solution: Find out the blocking points and replace the flow path components according to the step-by-step elimination method. 3. The Guard column pressure increases Solution: Replace the sieve plate at the inlet of the Guard column. 4.3 Large baseline noise 1.
②Voltage instability, or interference Solution: Install voltage stabilizer. 4.4 Large baseline drift 1. The preheating time of the instrument is not enough Solution: Extend preheating time. 2. Leak of the instrument Solution: Find the leak for repair. 3. Voltage instability or electrostatic interference Solution: Add voltage stabilizer and ground the instrument.
Solution: Observe whether the amount of liquid absorbed by the autosampler is normal. If it is not normal, please contact our customer service personnel for repair. 4.7 Abnormal suppressor current 1. Poor cable contact Solution: Replace power cord or constant current source. 4.8 No peak 1.
Solution: Reduce the sample concentration or replace the chromatograph column with high load capacity. 4.10 Poor degree of separation 1. Eluent The concentration of eluent is inappropriate. Solution: Select the appropriate eluent concentration. Excessive flow rate of eluent Solution: Select the appropriate flow rate. 2.
Solution: Replace the ultrapure water. 3. Flow path Pipeline leak Solution: Find the leak and tighten or replace the leaking parts. The flow path is blocked Solution: Find the blocked place, repair or replace it. 4. Changes in ambient temperature Solution: Keep the environment as constant as possible when conducting experiments.
4.13There are bubbles in infusion pump 1. Gas is adsorbed in flow path pipe Solution: Start the exhaust valve of the infusion pump and start the advection pump when water passes through, and constantly vibrate the filter head to remove the gas. 2.
5 Maintenance 5.1 Removal of blocking units When the flow path is blocked, the system pressure will increase or even exceed the bearing capacity of the infusion pump, resulting in unstable system or abnormal peak output. Therefore, the abnormal unit should be checked in time. When the system pressure does not exceed the bearing capacity of the infusion pump, the pipe joints can be removed from the REGEN OUT port of the suppressor in sequence according to the schematic diagram of the system flow path shown in Figure 2-8 to observe the system...
An inner diameter of 0.75mm Part of six-way valve connected with the Teflon pipe waste liquid An outside diameter of 1/8 inch Part connecting REGEN OUT port of the Teflon pipe suppressor Table 5-2 Joint uses Joint type Manual tightening PEEK Flow path connection Hex wrench Connecting the six-way valve interface...
reinstall the check valve and tighten the joint; 6. Remove bubbles in the pump and start the pump for test. Outlet check valve Inlet check valve Figure 5-1 Pump check valve position Direction of flow Coil Figure 5-2 Inlet direction of check valve Caution Only when the direction of the check valve is correct can the infusion pump drive the liquid to flow to the flow path, otherwise the liquid cannot be sucked in.
5.4 Replacement of sealing ring or plunger rod Damaged sealing ring or plunger rod will cause leak of pump body, unstable system flow path, increased baseline noise and other consequences. When such phenomena occur, replacement of sealing ring and plunger rod can be considered. The steps for replacing the pump sealing ring are as follows: 1.
2. Disconnect PEEK pipeline and joint; 3. Use a screwdriver to remove the three screws on the conductivity cell; 4. Remove the conductivity cell from the DB joint; 5. Install new conductivity cell aiming at the pinhole of DB joint; 6.
4. Loosen the fixing knob of the working electrode, clamp the clamping pins on both sides of the potential positioning block of the working electrode, pull the working electrode out of the positioning hole, and carefully remove it; 5. Use tweezers to peel off the old gasket from the cell body; 6.
5.8 Replacement of the suppressor New suppressors or suppressors that have not been used for a long time (more than one week) should be activated first. Activation step: 1. Connect the pump outlet directly to the REGEN IN port of the suppressor. Connect the REGEN OUT port directly to the waste liquid bottle.
Caution The joint of suppressor cable should be connected properly, otherwise no current will be applied. 5.9 Replacement of the power fuse 1. Turn off the main power switch of the instrument and unplug the "pin" power plug at the rear end of the instrument; 2.
clip Fuse fixing Fuse Figure 5-6 Replacement of the fuse Through the above operations, if the instrument returns to normal operation, the fuse replacement is completed. If there is no normal operation, please check other possible causes of abnormal operation of the instrument. 5.10 Replacement of the eluent generator storage tank When the instrument is equipped with an eluent generator, the operations to be performed when replacing eluent generator are as follows:...
4. Open the two plugs of the new eluent generation tank and connect them to the pipeline. These two joints can be connected at will irrespective of outlet or inlet; Figure 5-8 Plugs and cable of eluent generation tank 5. Connect the cable of the eluent generation tank completely; 6.
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Conductor cell OUT The eluent to trap column generation tank OUT REGEN IN. trap to the trap column column REGEN OUT IN and OUT to the to suppressor REGEN low-pressure IN. The connection degassing box. The sequence can be connection reversed.
B. Installation B.1 Equipment requirements 1. Ambient temperature: 5℃ to 30℃ 2. Relative humidity: relative humidity of 5% to 85%, non-condensing 3. The room should be clean and well ventilated. The instrument should be stably placed on the workbench without strong mechanical vibration and electromagnetic interference sources.
NOTE:Refer to section 3. B.4 Connecting the instrument to computer The DW-CIC-D100 instrument is connected with the computer through a data line. 1. Take out the data cable in the instrument accessory box; 2. Connect the DB fitting end of the data cable into the DB socket on the rear panel of the instrument, and connect the USB end into the USB socket of the computer end.
the autosampler injection valve and connect the valve port 3 to the inlet of the Guard column. And the connection of the other pipelines is the same as that when the autosampler is not connected. B.5.1 Steps for connecting SHA-9 to instrument trigger line 1.
Please refer to the user manual of SHA-16 autosampler for details. B.6 Connecting power cord Connect the power cord from the main power socket of the instrument rear panel to the grounding power supply. The instrument power supply is automatically induced, so the selected line voltage does not need to be adjusted.
B.8 Connecting waste liquid pipes DW-CIC-D100 instrument waste liquid pipe includes the following four types: 1. The back flushing waste liquid pipe of the advection pump discharges the waste liquid from the flushing pump head;...
1. Rinse the eluent bottle with ultrapure water; 2. Load the eluent into a eluent bottle; 3. Place the eluent bottle in the eluent tray at the upper end of DW-CIC-D100; 4. After installing the filter head, put the pipeline into the eluent bottle and tighten...
Caution DW-CIC-D100 instrument does not require pressure-protected eluent bottle. However, if the eluent is degassed manually or easily contaminated, it is recommended to equip the eluent bottle with nitrogen protection. B.10 Flushing pump Before flushing, ensure that the eluent bottle is full of eluent; the eluent bottle cap is tightened;...
1. Insert a 10 mL injector into the curved needle beside the exhaust valve; 2. Turn the exhaust valve counterclockwise for 1/4-1/2 turn to start the exhaust valve; 3. Draw the injector or control the flow rate of the pump to 1 mL/min, and slowly pump out the gas in the pipeline or the eluent before replacement;...
0.4MPa. B.13 Pressurization of eluent bottle (optional) DW-CIC-D100 instrument do not require a pressure-protected eluent bottle. However, if the eluent has been degassed manually or is easy to pollute, it is recommended to equip...
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