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Okolab MICA-CO2-INCUBATOR User Manual

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MICA-CO2-INCUBATOR
MICA-O2-UPGRADE
MICA-CO2-O2-INCUBATOR
User Manual
IST 2113_REV02
IST 2114_REV02
SV 1.0.0.0
SV 0.6.6

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Summary of Contents for Okolab MICA-CO2-INCUBATOR

  • Page 1 MICA-CO2-INCUBATOR MICA-O2-UPGRADE MICA-CO2-O2-INCUBATOR User Manual IST 2113_REV02 IST 2114_REV02 SV 1.0.0.0 SV 0.6.6...
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  • Page 3: Table Of Contents

    ............................ 12 ONTROL MICA-CO2-INCUBATOR-SENSOR-MODULE ..........................14 MICA-WATER-PUMP..................................14 MICA-WATER-BOTTLE-CAP ................................15 MICA-CO2-O2-INCUBATOR – C ..........................16 ONTROL MICA-CO2/O2-INCUBATOR-HM ..............................17 MICA-CO2-INCUBATOR - INSTALLATION ........................19 ..................................19 RELIMINARY OPERATIONS ................................... 21 ONTROL SSEMBLY MICA-CO2-INCUBATOR - C ....................... 24 ONFIGURATION SSEMBLY MICA-CO2-INCUBATOR –...

  • Page 5: Preface

    Control Unit. Following the same gas flow path, the humidity control is performed. Warm air from the Air Heater flows on the surface of a hot water reservoir included in the MICA-CO2-INCUBATOR-HM. The water reservoir is therefore heated up to generate the desired vapor content in the gas stream. It is automatically and periodically filled with water thanks to the MICA-WATER-PUMP, a peristaltic pump that delivers the water from the MICA-WATER-BOTTLE, the water tank, directly to the water reservoir.
  • Page 6 Temperature control is performed as in the MICA-CO2-INCUBATOR. Temperature can be set from 3°C above the ambient temperature to 45°C. The temperature is controlled always inside the microscope cabinet ambient. The temperature is raised by recirculating and warming air inside the microscope. Cold air from the microscope comes through the upper channel of the functional tubes, and enters in the Control Unit.

  • Page 7: Symbol Description

    Symbol Description This paragraph describes the symbols used in this manual and on the product label. Symbols used in this manual The following symbols identify important information to note: CAUTION or WARNING: this symbol warns you about the risk of electrical shock. CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that can affect the functionality of the instrument.

  • Page 8: Safety Notes

    While opening the microscope, keep a certain distance from the door. • Install an external gas detector to measure the CO concentration in room ambient. [1] For further information, it is available the Okolab White Paper Indoor CO Concentration Limit. • Transport the device with care •...

  • Page 9: Maintenance

    • Before starting an experiment: For MICA-CO2-INCUBATOR, check if the external and internal cover of the Cable Entry Tube is in place correctly. If one of the covers is missing, condensation may occur in the lower left corner of MICA sample compartment.
  • Page 10 We reserve the right to make technical variations. IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE...

  • Page 11: Supplied Equipment

    11. MICA-WATER-BOTTLE (see pointer 11, Figure 1). Glass bottle for water reservoir. 12. Power Cord (see pointer 12. Figure 1). To power the system on. 13. Micro USB cable (see pointer 13, Figure 1). To connect MICA-CO2-INCUBATOR to PC. This enables that MICA-CO2-INCUBATOR system is managed by LAS X software.
  • Page 12 Figure 1. MICA-CO2-INCUBATOR – Supplied Equipment. Figure 2 shows the supplied equipment for MICA-CO2-O2-INCUBATOR: 1. MICA-CO2-O2-INCUBATOR – Control Unit (see pointer 1, Figure 2) with MICA-CO2/O2- INCUBATOR-VALVE-KT embedded that contains the CO Sensor and the O Sensor. It also provides connection ports for MICA-TC and MICA-TK extensions.
  • Page 13 6 mm O.D.. To transport gas from MICA-CO2/O2-INCUBATOR-VALVE-KIT to MICA- CO2/O2-INCUBATOR-HM. 16. Power Cord (see pointer 16, Figure 2). To power the system on. 17. Micro USB cable (see pointer 17, Figure 2). To connect MICA-CO2-INCUBATOR to PC. This enables that MICA-CO2-O2-INCUBATOR system is managed by LAS X software.
  • Page 14 Figure 2. MICA-CO2-O2-INCUBATOR – Supplied Equipment. Figure 3 shows the supplied equipment for MICA-O2-UPGRADE: 1. MICA-CO2/O2-INCUBATOR-VALVE-KIT (see pointer 1, Figure 3). It contains the CO Sensor and the Sensor. It also provides connection ports for MICA-TC and MICA-TK extensions. 2. HUMIDIFIER-CHAMBER Tube (see pointer 2, Figure 3): silicon tube, 6mm O.D., 32 cm long. To transport gas from MICA-CO2/O2-INCUBATOR-HM to MICA-CO2/O2-GAS-CHAMBER.
  • Page 15 6. PUMP-BOTTLE Tube (see pointer 6, Figure 3): silicone tube, 6 mm OD, 30.5 cm long. To transport water from MICA-WATER-BOTTLE to MICA-WATER-PUMP. 7. OKO-AP-BL (see pointer 7, Figure 3). To supply air to MICA-CO2/O2-INCUBATOR-VALVE-KIT. 8. TUBE-DA (see pointer 8, Figure 3): Silicon tube, 7 mm O.D., with a PTFE filter in the middle connected at both ends to a blue rigid tube 6 mm O.D..

  • Page 16: Equipment Description

    6. Water Bottle connector (see pointer 6, Figure 4). To provide power to MICA-WATER-PUMP. 7. micro-USB cable connector (see pointer 7, Figure 4). 8. Humidifier connector (see pointer 8, Figure 4). To provide power to MICA-CO2-INCUBATOR-HM. 9. INCUBATOR-PUMP Tube connector (see pointer 9, Figure 4). To transport water from MICA-WATER- PUMP to MICA-CO2-INCUBATOR-HM.
  • Page 17 It is accommodated in a dedicated slot between the two channels. It has to be connected to the connector on the lateral panel of MICA-CO2-INCUBATOR-SENSOR-MODULE to enable electrical communication. 2. Latches (see pointer 2, Figure 5). To fix MICA-CO2-INCUBATOR to MICA microscope back panel. 3. MICA-CO2-INCUBATOR-VALVE-KIT (see pointer 3, Figure 5). It contains the CO on/off valve.

  • Page 18: Mica-Co2-Incubator-Sensor-Module

    Figure 6. MICA-CO2-INCUBATOR-HM MICA-CO2-INCUBATOR-SENSOR-MODULE Figure 7 shows MICA-CO2-INCUBATOR-SENSOR-MODULE connections: 1. External hooks (see pointer 1, A, Figure 7). To fix MICA-CO2-INCUBATOR-SENSOR-MODULE to the internal wall of MICA microscope. 2. MICA-CO2-INCUBATOR-SENSOR-MODULE Cable connector (see pointer 2, A, Figure 7). 3. micro-USB cable connector (see pointer 3, A, Figure 7). Connect the micro-USB cable to update MICA- CO2-INCUBATOR-SENSOR-MODULE software.

  • Page 19: Mica-Water-Bottle-Cap

    4. INCUBATOR-PUMP Tube connector (see pointer 4, Figure 8). To transport water from MICA-WATER- PUMP to MICA-CO2-INCUBATOR-HM. Figure 8. MICA-WATER-PUMP MICA-WATER-BOTTLE-CAP Figure 9 shows MICA-WATER-BOTTLE-CAP: 1. MICA-WATER-BOTTLE-CAP cable (see pointer 1, Figure 9). To be connected to MICA-WATER-PUMP to provide power to the Level Sensor - Water Bottle Underfill.

  • Page 20: Mica-Co2-O2-Incubator - Control Unit

    MICA-CO2-O2-INCUBATOR – Control Unit Figure 10 describes the bottom panel of MICA-CO2-O2-INCUBATOR: 1. MICA-CO2/O2-INCUBATOR-VALVE-KIT (see pointer 1, Figure 10). 2. TUBE-CQ connector (see pointer 2, Figure 10). Push to fit connector for 6 mm O.D. rigid tube. To transport gas from MICA-CO2/O2-INCUBATOR-VALVE-KIT to MICA-CO2/O2-INCUBATOR-HM. 3.

  • Page 21: Mica-Co2/O2-Incubator-Hm

    6. CO supply connector Push to fit connector for 6 mm O.D. rigid tube (see pointer 6, Figure 11). Figure 11. MICA-CO2-O2-INCUBATOR - Right view. MICA-CO2/O2-INCUBATOR-HM Figure 12 shows MICA-CO2/O2-INCUBATOR-HM connections: 1. External hooks (see pointer 1 in Figure 12). To fix MICA-CO2/O2-INCUBATOR-HM to the internal wall of MICA microscope.
  • Page 22 Front view Bottom view Side view Figure 12. MICA-CO2/O2-INCUBATOR-HM...

  • Page 23: Mica-Co2-Incubator - Installation

    3. Check if the external cover of the Cable Entry Tube is in place correctly (see Step 3, Figure 13). STEP 1 Unscrew and remove 2x COVER 4x M4x5 NECESSARY TOOL (NOT PROVIDED) #2 Philips screwdriver STEP 2 Unscrew and remove NECESSARY TOOL 4x M6x12 (NOT PROVIDED) 5.0 mm hexagonal key STEP3 Figure 13. MICA-CO2-INCUBATOR – Preliminary operations externally.
  • Page 24 5. Check if the internal cover of the Cable Entry Tube is in place correctly (see Step 5, Figure 14). STEP 4 2x COVER 2x M4x8 NECESSARY TOOL (NOT PROVIDED) 3.0 mm hexagonal key STEP 5 Figure 14. MICA-CO2-INCUBATOR – Preliminary operations internally.

  • Page 25: Control Unit - Assembly

    1. Open the two lateral latches of the Control Unit and separate it from MICA-SUPPORT-PANEL (see Step 1, Figure 15). Note ►MICA-SUPPORT-PANEL is secured to the Control Unit when the device is delivered from the Okolab factory. 2. Fix MICA-SUPPORT-PANEL to MICA microscope back panel using the Fixing Kit. Screw the four M6x40 spacers into the four holes of MICA microscope back panel using a 11 mm wrench.
  • Page 26 4. Clamp the Control Unit closing the two lateral latches (see Step 4, Figure 16). STEP 3 STEP 4 Figure 16. Control Unit – Assembly. Install the two MICA-FUNCT-TUBE-COVER as follows: 1. Unscrew and remove the M3x6 hexagonal head screw using a 2.5 mm hexagonal key (see Step 1, Figure 17) from each MICA-FUNCT-TUBE-COVER.
  • Page 27 STEP 1 Unscrew and remove 2x MICA-FUNCT-TUBE-COVER NECESSARY TOOL 2x M3x6 (NOT PROVIDED) 2.5 mm hexagonal key STEP 2 NECESSARY TOOL 1x M3x6 (NOT PROVIDED) 2.5 mm hexagonal key Figure 17. MICA-FUNCT-TUBE-COVER – Assembly.

  • Page 28: Mica-Co2-Incubator - Configuration Assembly

    MICA-CO2-INCUBATOR - Configuration Assembly To install MICA-WATER-PUMP correctly, follow the instructions below: 1. Remove the left panel of MICA support table by loosening and removing the three hexagonal head M3x10 screws using a 2.5 mm hexagonal key (see Step 1, Figure 18). Keep aside the screws you removed; they need to be used later.
  • Page 29 3. Secure MICA-WATER-PUMP to the MICA-PUMP-SHELF by fastening the two M3x6 hexagonal head screws using a 2.5 mm hexagonal key (see Step 3, Figure 18). 4. Remove the ferrite 74271131 from the cable of MICA-WATER-PUMP by inserting and gently pushing the safety key into its grooves (see Step 4, Figure 19).
  • Page 30 6. Plug the cable of MICA-WATER-PUMP to the port labeled “Water Bottle” placed on the Power input panel of Control Unit (see Step 6, Figure 20). STEP 5 STEP 6 Figure 20. MICA-WATER-PUMP – Connection. Note ►Plug the connector of the electrical cable to the Power input panel of Control Unit, keeping the flat part facing outwards (see Figure 21).
  • Page 31 Figure 21. Connection of electrical cable’s connector to Power input panel. Follow the instructions below to install MICA-WATER-BOTLLE-CAP correctly: 1. Introduce MICA-WATER-BOTLLE-CAP in the internal slot of the left leg on the front of MICA support table (see Step 1, Figure 22). 2.
  • Page 32 MICA-WATER-BOTTLE-CAP and large connector placed on the bottom panel of the MICA- CO2-INCUBATOR-HM (see Step 2, Figure 23). Note ► MICA-CO2-INCUBATOR-HM is screwed to the bottom panel of the Control Unit when the device is delivered from the Okolab factory.
  • Page 33 STEP 1 MICA-CO2-INCUBATOR-TUBE-KIT 1x PUMP-BOTTLE Tube STEP 2 MICA-CO2-INCUBATOR-TUBE-KIT 1x INCUBATOR-BOTTLE Tube Figure 23.PUMP-BOTTLE Tube and INCUBATOR-BOTTLE Tube – Connection. 3. Connect the INCUBATOR-PUMP TUBE (silicone tube, 6 mm OD, 155 cm long) between the “To Incubator” connector of MICA-WATER-PUMP and small connector placed on the bottom panel of MICA-CO2-...
  • Page 34 1. Fill MICA-WATER-BOTTLE with bi-distilled or autoclaved water (do not exceed 1 liter). Note ► Okolab suggests adding 1 ml of non-volatile antibacterial product in the water solution. Refill the antibacterial product after the effective period indicated by its operating instructions. The antibacterial product has to be effective against the growth of microorganisms (bacteria, fungi, and yeasts) in a tepid water reservoir.
  • Page 35 1x MICA-WATER-BOTTLE STEP 2 Figure 25. MICA-WATER-BOTTLE – Assembly. Use plastic ties to cinch around the MICA-CO2-INCUBATOR-TUBE-KIT and the cable of MICA-WATER- PUMP (see Figure 26). Do not tightly bundle the tubes with the plastic tie to avoid throttling the tubing.
  • Page 36 MICA-FUNCT-TUBE are visible (see Step 1, Figure 27). The MICA-FUNCT-TUBE on the left side of the condenser is equipped with the MICA-CO2-INCUBATOR-SENSOR-MODULE cable. To avoid damages during transport, the cable has been placed inside the MICA-FUNCT-TUBE (see Step 1, A, Figure 27).
  • Page 37 1. Remove the four protective caps placed on the internal wall of MICA microscope (see Step 1, Figure 28). 2. Place MICA-CO2-INCUBATOR-SENSOR-MODULE on the internal wall of MICA microscope. Make sure to position the MICA-CO2-INCUBATOR-SENSOR-MODULE’s external hooks to match the dedicated grooves...
  • Page 38 3. Gently press MICA-CO2-INCUBATOR-SENSOR-MODULE downwards to lock the MICA-CO2-INCUBATOR- SENSOR-MODULE’s external hooks (see Step 3, Figure 29). 4. Plug MICA-CO2-INCUBATOR-SENSOR-MODULE cable to the port placed on the side panel of MICA-CO2- INCUBATOR-SENSOR-MODULE (see Step 4, Figure 29). Tip ►Plug MICA-CO2-INCUBATOR-SENSOR-MODULE cable’s connector with the flat part facing inwards. If the connector does not fit, do not force the plug into the connection.
  • Page 39 Follow the steps below to install MICA-TC correctly: 1. Connect MICA-TC to the white port placed on the bottom panel of the MICA-CO2-INCUBATOR-SENSOR- MODULE (see Step 1, Figure 30). MICA-TC has two connector pins of different dimensions. Make sure to follow the proper direction of entry into the openings of MICA-CO2-INCUBATOR-SENSOR-MODULE’s...
  • Page 40 INCUBATOR-SENSOR-MODULE (see Figure 31). MICA-TK has two connector pins of different dimensions. Make sure to follow the proper direction of entry into the openings of MICA-CO2-INCUBATOR-SENSOR-MODULE’s green connector. Insert the widest connector pin of MICA-TK into the left opening of MICA-CO2-INCUBATOR-SENSOR-...
  • Page 41 Figure 31. MICA-TK – Connection to MICA-CO2-INCUBATOR-SENSOR-MODULE. Connect the MICA-CO2-INCUBATOR-HM cable to the “Humidifier” port on the Power input panel of MICA- CO2-INCUBATOR (see Figure 32). Figure 32. MICA-CO2-INCUBATOR-HM – Connection. Connect the micro-USB cable between the “USB” micro port on the Power input panel of the MICA-CO2- INCUBATOR (keep the USB symbol facing outwards) to the USB port of Las X PC (see Figure 33).

  • Page 42: Mica-Co2-Incubator - Gas Connection

    Rear view Figure 34.MICA-CABLE-SUPPORT – Installation for MICA-CO2-INCUBATOR. MICA-CO2-INCUBATOR – Gas connection Install a pressure gauge (not supplied by Okolab) for the CO line between the CO supply and MICA-CO2- INCUBATOR. Connect the CO supply tube to the push to fit connector (for 6 mm O.D. rigid tube) on the side panel of MICA-CO2-INCUBATOR-VALVE-KIT (see Figure 35).

  • Page 43: Mica-Co2-Incubator - Start Up

    1. Connect the Power Cable between the Power Input port on the Power input panel of MICA-CO2- INCUBATOR (see Step 1, Figure 36) and the power main socket. 2. To switch MICA-CO2-INCUBATOR on put the Power Switch, placed on the Power input panel of MICA- CO2-INCUBATOR, on the “ON” position (see Step 2, Figure 36).
  • Page 44 Figure 36. Power Connection.

  • Page 45: Mica-O2-Upgrade - Installation

    INCUBATOR-PUMP Tube and INCUBATOR-BOTTLE Tube may contain water. Check for the presence of remaining water before disconnecting them. 1. Switch MICA-CO2-INCUBATOR off by placing the Power Switch button on “OFF” position (see Step 1, Figure 37). 2. Disconnect the Power Cable from the Power Input port on the Power input panel of MICA-CO2- INCUBATOR and from the power main socket (see Step 2, Figure 37).
  • Page 46 3.0 mm hexagonal key Figure 37. MICA-O2-UPGRADE – Preliminary operations – part 1. 9. Remove MICA-CABLE-SUPPORT from the Power input panel of MICA-CO2-INCUBATOR by loosening and removing the two M3x6 cross-head screws using a #1 Phillips screwdriver. Unplug micro-USB cable,...
  • Page 47 MICA-WATER-PUMP cable, MICA-CO2-INCUBATOR-HM cable from the Power input panel of MICA-CO2- INCUBATOR (see Step 9, Figure 38). 10. Remove the INCUBATOR-PUMP Tube (silicone tube, 6 mm O.D., 155 cm long) and INCUBATOR-BOTTLE Tube (silicone tube, 10 mm O.D., 190 cm long) from connectors placed on the bottom of the MICA-CO2- INCUBATOR-HM (see Step 10, Figure 38).

  • Page 48: Control Unit - Assembly

    14. Remove MICA-SUPPORT-PANEL by loosening and removing the four M6x10 cross-head screws on its internal panel with a #3 Phillips screwdriver (see Step 14, Figure 39). 15. Remove the external cover of the Cable Entry by loosening and remove the two M4x8 cross-head screws using a #2 Phillips screwdriver (see Step 15, Figure 39).

  • Page 49: Mica-O2-Upgrade - Configuration Assembly

    2. Extract the MICA-CO2-INCUBATOR-VALVE-KIT from the side of MICA-CO2-INCUBATOR (see Step 2, Figure 40). 3. Remove the blue rigid tube (6 mm O.D.) from the internal push to fit connector of MICA-CO2-INCUBATOR- VALVE-KIT and detach the connector cable (see Step 3, Figure 40).
  • Page 50 3. Fasten the six M3x6 cross-head screws placed on the bottom panel of the MICA-CO2/O2-INCUBATOR- VALVE-KIT using a #1 Phillips screwdriver (see Step 3, Figure 41). STEP 1 STEP 2 MICA-CO2/O2- INCUBATOR-VALVE-KIT STEP3 NECESSARY TOOL 6x M3x6 (NOT PROVIDED) #1 Philips screwdriver Figure 41.
  • Page 51 STEP 1 STEP 2 Unscrew and remove NECESSARY TOOL 3x M3x10 (NOT PROVIDED) 2.5 mm hexagonal key STEP 3 STEP 4 NECESSARY TOOL NECESSARY TOOL 2x M4x8 2x M3x6 (NOT PROVIDED) (NOT PROVIDED) 3.0 mm hexagonal key 2.5 mm hexagonal key Figure 42.
  • Page 52 STEP 5 STEP 6 Unscrew and remove 3x M3x8 NECESSARY TOOL NECESSARY TOOL 1x OKO-AP-BL 3x M3x10 (NOT PROVIDED) (NOT PROVIDED) 2.5 mm hexagonal key #1 Philips screwdriver STEP 7 STEP 8 NECESSARY TOOL NECESSARY TOOL 2x M4x8 2x M4 2x M3x6 (NOT PROVIDED) (NOT PROVIDED)
  • Page 53 the cable of MICA-WATER-PUMP and OKO-AP-BL. Connect the other end to the air inlet push to fit connector of MICA-CO2/O2-INCUBATOR-VALVE-KIT (see Step 10, C, Figure 44). STEP 9 STEP 10 1x TUBE-DA Figure 44. MICA-WATER-PUMP and OKO-AP-BL – Connection. 11. Plug the cable of MICA-WATER-BOTLLE-CAP to the port labeled “Water Level Sensor” of MICA-WATER- PUMP (see Step 11, A, Figure 45).
  • Page 54 Figure 45. MICA-WATER-BOTTLE-CAP – Connection. To mount MICA-FUNCT-TUBE-DEF on MICA-FUNCT-TUBE follow the instructions below: 1. Check that the O-ring of MICA-FUNCT-TUBE-DEF is positioned in its housing firmly (see Step 1, Figure 46). 2. Open MICA microscope door to access the MICA sample compartment. On the MICA internal wall, the two openings of the MICA-FUNCT-TUBE are visible (see Step 2, Figure 46).
  • Page 55 1. Place MICA-CO2/O2-INCUBATOR-HM on the internal wall of MICA microscope. Make sure to position the MICA-CO2/O2-INCUBATOR-HM’s external hooks to match the dedicated grooves on the wall (see Step 1, Figure 47). 2. Gently press MICA-CO2/O2-INCUBATOR-HM downwards to lock the MICA-CO2/O2-INCUBATOR-HM’s external hooks (see Step 2, Figure 47).
  • Page 56 4. Pass the TK-PROBE and the TC-PROBE through the Cable Entry Tube (see Step 4, A, Figure 48). Connect the TK-PROBE of the MICA-CO2/O2-INCUBATOR-HM to the green connector placed on the bottom panel of MICA-CO2/O2-INCUBATOR-VALVE-KIT (see Step 4, B, Figure 48). Connect the TC-PROBE of the MICA- CO2/O2-INCUBATOR-HM to the white connector placed on the bottom panel of MICA-CO2/O2- INCUBATOR-VALVE-KIT (see Step 4, B, Figure 48).
  • Page 57 Do not tightly bundle the cable with the plastic tie to avoid MICA-TC damage. STEP 5 1x MICA-TC STEP 6 NECESSARY TOOL (NOT PROVIDED) 1x Plastic Tie Figure 49. MICA-TC – Connection. Note ►If you need to use MICA-TK, connect it to the green port placed on the bottom panel of MICA-CO2/O2- INCUBATOR-HM (see Figure 50).
  • Page 58 Proceed with the following steps to complete MICA-WATER-PUMP and OKO-AP-BL installation: 1. Connect one end of INCUBATOR-PUMP Tube (silicone tube, 6mm OD, 155 cm long) to the “To Incubator” barbed connector of MICA-WATER-PUMP (see Step 1, A, Figure 51). Let the free end of the tube pass through the Cable Entry Tube (see Step 2, B, Figure 51).
  • Page 59 Use plastic ties to cinch around the MICA-CO2-INCUBATOR-TUBE-KIT, the cable of MICA-WATER-PUMP and OKO-AP-BL and TUBE-DA (see Figure 52). Do not tightly bundle the tubes with the plastic tie to avoid throttling the tubing. NECESSARY TOOL (NOT PROVIDED) Figure 52. MICA-O2-UPGRADE - Cable management.
  • Page 60 1x TUBE-CQ Figure 53. TUBE-CQ – Installation. Tip ►Let the silicon end of TUBE-CQ pass through the left opening of MICA-CO2/O2-INCUBATOR-HM before being connected to the left barbed connector placed on the top panel of MICA-CO2/O2-INCUBATOR-HM (see Figure 54). Figure 54. TUBE-CQ – Opening. 2.
  • Page 61 1x HUMIDIFIER- CHAMBER Tube Figure 55. HUMIDIFIER-CHAMBER Tube – Connection. Insert MICA-CO2/O2-SPONGE-CAP into the internal opening of the Cable Entry Tube. The sponge cap helps to accommodate cables and tubes that pass through it and avoid any leakage of warm air (see Figure 56). Internal side External side 1x MICA-CO2/O2-...

  • Page 62: Mica-O2-Upgrade - Gas Connection

    #1 Philips screwdriver Rear view Figure 57. MICA-CABLE-SUPPORT – Installation for MICA-O2-UPGRADE. MICA-O2-UPGRADE – Gas connection Install a pressure gauge (not supplied by Okolab) for the CO line between the CO and N supply and the Control Unit. Connect the CO supply tube to the push to fit connector (for 6 mm O.D.

  • Page 63: Mica-O2-Upgrade - Start Up

    N Supply - Connection. MICA-O2-UPGRADE – Start up To switch MICA-CO2-INCUBATOR with MICA-O2-UPGRADE on following the steps below: Before starting an experiment: • Check that all water/gas tubing ends are well inserted into the connectors so they cannot slip off.
  • Page 64 Figure 59. Power Connection.

  • Page 65: Mica-Co2-O2-Incubator Installation

    MICA-CO2-O2-INCUBATOR Installation Preliminary Operations Before assembling the Control Unit, follow the steps below: 1. Remove both external covers for MICA-FUNCT-TUBE on MICA microscope back panel by loosening and removing the two M4x5 cross-head screws each with a #2 Phillips screwdriver (see Step 1, Figure 60). 2.
  • Page 66 STEP 1 Unscrew and remove 2x COVER 4x M4x5 NECESSARY TOOL (NOT PROVIDED) #2 Philips screwdriver STEP 2 Unscrew and remove NECESSARY TOOL 4x M6x12 (NOT PROVIDED) 5.0 mm hexagonal key STEP 3 Unscrew and remove 1x COVER 2x M4x8 NECESSARY TOOL (NOT PROVIDED) #2 Philips screwdriver...

  • Page 67: Control Unit - Assembly

    STEP 4 2x COVER 2x M4x8 NECESSARY TOOL (NOT PROVIDED) 3.0 mm hexagonal key STEP 5 Unscrew and remove 1x COVER 1x M4x8 NECESSARY TOOL (NOT PROVIDED) 3.0 mm hexagonal key Figure 61. MICA-CO2-O2-INCUBATOR – Preliminary operations internally. Control Unit – Assembly For the Control Unit assembly refer to paragraph 6.2.
  • Page 68 Unscrew and remove 3x M3x10 NECESSARY TOOL (NOT PROVIDED) 2.5 mm hexagonal key Figure 62. MICA Support Table – Left panel – removal. 2. Loosen and remove the three M3x8 cross-head screws placed on the bottom panel of OKO-AP-BL using a #1 Phillips screwdriver (see Step 2, Figure 63).
  • Page 69 STEP 2 STEP 3 Unscrew and remove 3x M3x8 NECESSARY TOOL NECESSARY TOOL 1x OKO-AP-BL 3x M3x10 (NOT PROVIDED) (NOT PROVIDED) 2.5 mm hexagonal key #1 Philips screwdriver STEP 4 STEP 5 NECESSARY TOOL NECESSARY TOOL 2x M4x8 2x M4 2x M3x6 (NOT PROVIDED) (NOT PROVIDED)
  • Page 70 STEP 6 NECESSARY TOOL (NOT PROVIDED) Safety Key Figure 64. MICA-WATER-PUMP cable – Ferrite - Removal. 7. Pass the cable of MICA-WATER-PUMP and of OKO-AP-BL through the dedicated opening in the opposite table’s leg (see Step 7, A, Figure 65). Run the end of the cables out of the table’s leg. Coil the cable of MICA- WATER-PUMP around the ferrite 74271131 core with two turns close to the its connector (minimum distance 1 cm) and clamp the ferrite 74271131 (see Step 7, B, Figure 65).
  • Page 71 STEP 7 STEP 8 1x TUBE-DA Figure 65. MICA-WATER-PUMP and OKO-AP-BL – Connection. Note ►Plug the connector of the electrical cable to the Power input panel of Control Unit, keeping the flat part facing outwards (see Figure 21). If the connector does not fit, do not force the plug into the connection. Check if the connector has the correct mounting direction.
  • Page 72 STEP 1 1x MICA-WATER-BOTTLE- STEP 2 Figure 66. MICA-WATER-BOTTLE-CAP – Installation. 3. Connect PUMP-BOTTLE Tube (silicone tube, 6 mm OD, 30.5 cm long) between the small barbed connector MICA-WATER-BOTTLE-CAP and the “To Bottle” connector of MICA-WATER-PUMP (see Step 1, Figure 67). Let PUMP-BOTTLE Tube do the same path of the cable of MICA-WATER-BOTTLE-CAP.

  • Page 73: Mica-Co2-O2-Incubator - Gas Connection

    Remove four protective caps placed on the internal wall of MICA microscope (see Figure 68). Figure 68. MICA-CO2/O2-INCUBATOR-HM – Protective caps – Removal. To proceed with MICA-CO2-INCUBATOR installation, refer to pp. 50 – 57. MICA-CO2-O2-INCUBATOR – Gas Connection For MICA-CO2-O2-INCUBATOR gas connection refer to paragraph 7.4.

  • Page 74: User Interface

    Figure 69. LAS X Hardware Configurator – Hardware Setup. 3. In the Microscope tab open the drop-down Environmental Control menu (see pointer 1, Figure 70). Okolab should appear automatically as selectable equipment. Flag the box (see pointer 2, Figure 70) and press OK...

  • Page 75: Climate Setup

    Figure 70. LAS X Hardware Configurator – Hardware Setup – Environmental Control. Note ► If you press on Apply button in Figure 70, you will save the settings without closing the Hardware setup submenu. Climate setup To define the Climate settings, follow the instructions below: 1.

  • Page 76: Temperature Calibration

    To start the temperature calibration, follow the instructions below: 1. Make sure that MICA-TK, the fine gauge thermocouple, and MICA-TC, the NTC thermistor, are connected to the MICA-CO2-INCUBATOR-SENSOR-MODULE for MICA-CO2-INCUBATOR configuration (see paragraph 6.3) or MICA-CO2/O2-INCUBATOR-HM for MICA-CO2-O2-INCUBATOR configuration (see paragraph 7.3).

  • Page 77: Temperature Control

    9.1.2.2 Temperature Control Okolab Control Unit can work in two alternative modes for the temperature control: Cage Mode and Sensor Mode. Note ► The Sensor Mode is set by default. Press the Cage Mode (see pointer 1, Figure 75) or Sensor Mode (see pointer 2, Figure 75) button to select one of temperature control modes.

  • Page 78: Las X

    In Cage Mode, the temperature inside the MICA sample compartment is controlled using as reference the reading of the MICA-TC to maintain the sample at the desired temperature. As the reading value of MICA-TC is dependent on its position inside the MICA sample compartment, it is crucial to place it in the dedicated position correctly (see Figure 30).

  • Page 79: Status Indicator

    Status Indicator The Status Indicator can display three different statuses, which are represented by the following Figures. Figure 77 shows the Status Indicator while the system is in the startup phase. When the startup routine is finished, the Status Indicator turns green, advising that the Setpoints have been reached (Figure 78). Figure 77.

  • Page 80: How To Enter The Set Points

    How to enter the set points To input a new temperature set point, click on + and – in the corresponding tab (see Figure 80). Alternatively, it is possible to enter the set point scrolling the temperature sliding bar (see Figure 81), or clicking on the temperature value (Figure 82) to type the new set point value.
  • Page 81 recovered while the control works to reach its target value. At steady state, the displayed temperature will correspond to the temperature measured at the Sample, considering the calibration. To input a new CO set point, click on + and – in the corresponding tab (see Figure 83). Alternatively, it is possible to enter the set point scrolling the CO sliding bar (see Figure 84), or clicking on the CO % value (see...

  • Page 82: Acquisition Tab

    After any change in the set point value, the controller enters into a transient regime, and as result the Status Indicator turns red (see Figure 79). Note ► The Relative Humidity set point cannot be set by the user. The Relative Humidity set point is automatically determined based on the temperature set point.

  • Page 83: Cleaning

    Before any cleaning operations, perform the Shutdown procedure. Switch Okolab Incubator off and unplug the main power cord. Before any cleaning operations, make sure that the inlet gases are not supplied to Okolab Incubator. Okolab Incubator’s external surfaces can be cleaned as follows: •...
  • Page 84 While mounting, do not turn the MICA-CO2-INCUBATOR-HM by force if too much resistance is encountered. If this should occur, the MICA-CO2-INCUBATOR-HM may be not aligned perfectly. Unscrew and remove it. Try again to screw the MICA-CO2-INCUBATOR-HM aligning it to the surface of the bottom panel of MICA-CO2-INCUBATOR.

  • Page 85: Troubleshooting

    Verify the Humidity Sensor reading using an external calibrated humidity meter. A1_c Humidity far from set point • Make sure that the microscope door is closed. • MICA-CO2-INCUBATOR-HM could be damaged. Contact Leica Customer Service to receive assistance. If the trouble persists, contact Leica Customer Service to receive assistance.
  • Page 86 Service to receive assistance. MICA-CO2-INCUBATOR • Check if MICA-CO2-INCUBATOR-HM is connected properly. Humidifier Module filling failed • MICA-CO2-INCUBATOR-HM could be damaged. Contact Leica Customer Service to receive assistance. MICA-CO2-O2-INCUBATOR • Check if MICA-CO2/O2-INCUBATOR-HM is connected properly. • MICA-CO2/O2-INCUBATOR-HM could be damaged. Contact Leica Customer Service to receive assistance.
  • Page 87 MICA-CO2-INCUBATOR & MICA-CO2-O2-INCUBATOR AC voltage detection error • Contact Leica Customer Service to receive assistance. MICA-CO2-INCUBATOR • MICA-CO2-INCUBATOR-SENSOR-MODULE could be damaged. Contact Leica Customer Service to receive assistance. Sensor broken MICA-CO2-O2-INCUBATOR • MICA-CO2/O2-INCUBATOR-VALVE-KIT could be damaged. Contact Leica Customer Service to receive assistance.
  • Page 88 • Check if MICA-CO2-INCUBATOR-HM is connected properly. Humidifier Heater Temperature far • MICA-CO2-INCUBATOR-HM could be damaged. Contact Leica Customer from set point Service to receive assistance. If the trouble persists, contact Leica Customer Service to receive assistance. MICA-CO2-INCUBATOR & MICA-CO2-O2-INCUBATOR •...
  • Page 89 OKO-AP-BL lifetime is expired. Contact Leica Customer Service to receive assistance. MICA-CO2-O2-INCUBATOR MICA-CO2-INCUBATOR-SENSOR- • Check if MICA-CO2-INCUBATOR-SENSOR-MODULE is still connected in MICA- MODULE still connected in MICA- CO2-O2-INCUBATOR configuration. CO2-O2-INCUBATOR If the trouble persists, contact Leica Customer Service to receive assistance.

  • Page 90: Technical Specification

    Microscope Environmental Control Conditions Temperature range 5°C above ambient temperature - 45°C Humidity range 53-67% range 0-10% Power Supply Supply voltage 110V / 230V Fuses 7AF 250V 5x20 Nominal frequency 50/60 Hz Power consumption 590 W max Table 2. MICA-CO2-INCUBATOR – Technical specifications.
  • Page 91 MICA-CO2-O2-INCUBATOR - Technical Specification MICA-TC NTC Thermistor Temperature range 5°C above ambient temperature - 45°C (ideal room temperature 23°C) Temperature accuracy ±0.10 °C Relative Humidity Sensor Humidity and Temperature Sensor IC Humidity Range Automatically saturated according to working temperature Humidity accuracy Not controlled in this mode Sensor Non-Dispersive InfraRed (NDIR) dual wave length detector...

  • Page 92: Support

    13 Support If your system is no longer functioning properly, contact your Leica representative. You can find information on worldwide Leica representatives on the Leica website: www.leica-microsystems.com. You may also contact our support team through the contact form here: https://www.leica- microsystems.com/de/service/...
  • Page 93 Figure 24. INCUBATOR-PUMP Tube – Connection................................30 Figure 25. MICA-WATER-BOTTLE – Assembly..................................31 Figure 26. MICA-CO2-INCUBATOR-TUBE-KIT & MICA-WATER-PUMP’s cable – Management..............32 Figure 27. MICA-FUNCT-TUBE-DEF – Assembly................................33 Figure 28. MICA-CO2-INCUBATOR-SENSOR-MODULE – Installation - Part 1...................... 34 Figure 29.
  • Page 94 Figure 87. LAS X – Acquisition tab – Tooltip..................................78 Figure 88. MICA-CO2-INCUBATOR-HM – Removal................................80 15 Table List Table 1. Alarm code and description......................................85 Table 2. MICA-CO2-INCUBATOR – Technical specifications............................86 Table 3. MICA-CO2-O2-INCUBATOR – Technical specifications..........................87...

  • Page 95: Manual Revision Table

    16 Manual Revision Table Revision Number Additions or changes Date First Emission September 2021...

  • Page 96: Warranty

    Okolab S.r.l. Okolab S.r.l. warrants only the parts manufactured by Okolab S.r.l to be free of defects. Okolab S.r.l. makes no other warranties or representations of any kind whatsoever, express or implied, except that of title, and all implied warranties including any warranty of merchantability and fitness for a particular purpose are hereby disclaimed.

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