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Okolab H301-T-UNIT-BL-PLUS Manual

Electric top stage incubation system, bold line
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H301-T-UNIT-BL-PLUS
Electric Top Stage Incubation System
Manual
Vers. 01.17
1

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Summary of Contents for Okolab H301-T-UNIT-BL-PLUS

  • Page 1 H301-T-UNIT-BL-PLUS Electric Top Stage Incubation System Manual Vers. 01.17...
  • Page 2 This page was left blank...

  • Page 3: Table Of Contents

    Temperature Controller connection ports ................10 H301-T-UNIT-BL-PLUS. INSTALLATION AND USER GUIDE ................. 10 H301-T-UNIT-BL-PLUS. Installation guide ................10 How to connect H301-T-UNIT-BL-PLUS to a BOLD LINE Digital Gas Controller ........13 USER INTERFACE ............................. 15 Home page ........................15 Colors meaning ......................15 6.1.1...
  • Page 4 6.2.2.5 Alarms ..........................31 6.2.2.6 Visual Effects ........................31 Overview Page ........................ 32 Glance Mode ........................32 CALIBRATIONS ..............................34 Chamber Self-Calibration ....................34 Objective Heater Manual Calibration ..................35 Objective Heater Self-Calibration ..................37 MAINTENANCE ..............................39 SUPPORT ................................40 QR Codes for Support ......................

  • Page 5: Preface

    H301-T-UNIT-BL-PLUS controls the temperature of the incubator base and lid and, when connected, of the Objective Heater. To improve accuracy on sample temperature, H301-T-UNIT-BL-PLUS is equipped with a flexible fine gauge thermocouple that can be used to provide feedback from the temperature of a reference well located in close proximity to the experimental sample.

  • Page 6: Symbols Description

    2 SYMBOLS DESCRIPTION The following symbols identify important information to note: CAUTION or WARNING: This symbol warns you about the risk of electrical shock. CAUTION or WARNING or IMPORTANT: This symbol warns you of circumstances or practices that can affect the instrument’s functionality. Please refer to accompanying documents. Tip - Supplies you with helpful suggestions.

  • Page 7: Safety Notes

    OTES Before operating the equipment please read carefully the instructions and the safety notes. If you have any questions, please contact Okolab. The equipment must only be used as intended and as described in this Manual. Equipment should only be operated by technically qualified personnel.
  • Page 8 We reserve the right to make technical variations. IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE, LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY...

  • Page 9: System Overview

    2. Fine Gauge Thermocouple. To measure specimen temperature 3. NTC Thermistor. To measure ambient temperature 4. #1 24V-DC Power Adapter 4.2.1 Equipment Required • Okolab incubating chamber with heated lid and base, chamber riser with one or more plate adapters 4.2.2 Optional • OKO-TOUCH, including: 1.

  • Page 10: Temperature Controller Connection Ports

    7. General Purpose Input Output (GPIO) port. 8. NTC Thermistor port. 9. Gauge Thermocouple port. H301-T-UNIT-BL-PLUS. I NSTALLATION AND USER GUIDE The following paragraphs illustrate how to install and use the Temperature Control Unit. The following instructions will guide you through a quick installation. For safe operation of the...
  • Page 11 Figure 2. H301-T-BL-PLUS. Standard Connection 1. Connect the base of the incubation chamber to the port labeled ‘Base’ on the rear panel of the Temperature Control Unit (see Figure 2). 2. Connect the lid of the incubation chamber to the port labeled ‘Lid’ on the rear panel of the Temperature Control Unit (see Figure 2).
  • Page 12 5. When using oil immersion objectives an Objective Heater should also be purchased. If your system is also equipped with the Objective Heater, (OBJ-COLLAR ####, optional) its objective collar should be installed before connecting the heater to rear panel of the Temperature Control Unit. Follow the steps shown in Figure 3.

  • Page 13: How To Connect H301-T-Unit-Bl-Plus To A Bold Line Digital Gas Controller

    Tip ►Wait until system has reached the steady state before loading your samples. The H301-T-UNIT-BL-PLUS can be used as a stand-alone device or in combination with any one of the Bold Line Gas Controllers. Figure 6 shows how to assemble the H301-T UNIT-BL-PLUS, for example, with a CO2-...
  • Page 14 H301-T-UNIT-BL-PLUS. Units rear panels. H301-T-UNIT-BL-PLUS. 3D view. Figure 6. CO2-O2 UNIT-BL [0-10; 1-18] and H301-T-UNIT-BL-PLUS The T-Unit must stack on top of the Gas Controller by lining up the bus ports located on the top and bottom surface of each. When the units are properly connected the Temperature, CO2 and O2 parameters will appear on the OKO-TOUCH Touch Screen interface Home page.

  • Page 15: User Interface

    SER INTERFACE Current Temperature value Temperature set-point Temperature chart Touch here to know generic info about Control Unit and running time Touch here to access system options and settings Touch here to go to Chart history lenght the OVERVIEW page set at 1 h Maximum T °C reached within the time-period Minimum T °C reached within the time-period...

  • Page 16: Changing Set Points

    The RED indicator means that there could be a problem with the unit. If after 20-30 minutes the Red light does not turn to Green, turn the system off, wait 5 minutes and turn it back on. If the problem persists contact Okolab at http://www.oko-lab.com 6.1.2 Changing Set Points To change a set point, simply touch the corresponding tab.

  • Page 17: Control Mode

    Figure 10. Chamber & Adapter change Figure 11 Chamber & Adapter settings Figure 12. Chamber and adapters saving Tip ►The system is shipped from the factory already set according to the incubating chamber model and sample adapter you have ordered. Keep in mind that thermal control settings change when changing the adapter in use.
  • Page 18 Figure 13. Chamber Mode Setting In this configuration the temperatures of both the lid and the base of the incubating chamber are strictly controlled. A careful calibration performed in our laboratories between these temperatures and that of the sample, guarantees that sample temperature is maintained at the desired set point value. The advantage of this solution is that you don’t have to manually place the Fine Gauge Thermocouple into a Reference Well.
  • Page 19 C. If the Custom Calibration was performed with a different set point temperature than what you plan on using (e.g Custom Calibration had set point of 37C but new experiment requires a set point temperature of 39C). 2. Use Factory settings optimized at 23°C Room Temperature. This is acceptable if your room temperature is within 1°C from 23°C.
  • Page 20 Figure 17. Sample Mode setting In this configuration, every time you run an experiment, you must place the Fine Gauge Thermocouple (thin green wire) into a Petri dish or well adjacent to your sample (from here on this will be referred to as Reference Well).

  • Page 21: Objective Heater

    Figure 19. Incubator Closed 6.2.1.3 Objective Heater Figure 20 shows how to enable/disable the Objective Heater (OBJ COLLAR ####). The Objective Heater is disabled by default. Read this paragraph only if your system is equipped with Objective Heater (OBJ COLLAR ####).
  • Page 22 offset. See Figure 21 above. This means that if the temperature is set at 37°C the Objective Heater is kept at 40°C in order to maintain the sample at the desired temperature while imaging with an oil immersion objective. To accept this offset you can press “USE FACTORY” and return to the Homepage. Alternatively the following options are available: 1.
  • Page 23 Figure 24. Self-calibration Warning in Chamber Mode Figure 25 shows the warning message that will appear once you have pressed “Calibrate” if you are working in Sample Feedback Mode. This message appears only if the Fine Gauge Thermocouple is far from the Set Point value.

  • Page 24: Thermal Cycles

    In the Objective Heater Calibration progress window press “Options” to access “Advanced Calibration Parameters”. In this window you can adjust Accuracy and Standard Deviation, which will be calculated over 10 minutes of readings. This allows setting more stringent parameters to the objective heater calibration. Figure 27.
  • Page 25 You can decide whether setting a new thermal cycle (“New”) or recalling a previously saved one (“Load”). To set a new thermal cycle, first choose the two temperatures between which you want to switch (SP1: Set Point 1 and SP2: Set Point 2). Next, set the duration in hours and minutes you want the system to dwell on each temperature.

  • Page 26: Open Incubator

    Figure 32. Cycle Start Time At set time the cycle will start. A thermal cycling icon will be displayed on the Homepage as a reminder. Trying to change set points or starting a new thermal cycle while a different one is still running will open the window in Figure 33.

  • Page 27: Ramp Rate

    Opening the incubator can lead to controller instability since both the specimen and the inside of the chamber are exposed to the external environment. In order to avoid the problem, UNO CONTROL UNIT has to stay in a holding state until the incubator is closed and its environment is in a steady state.

  • Page 28: Status

    Temperature’ page will open, next select ‘Status’. On this page you can see the status of the Temperature Control Unit, with a summary of all parameters allowing you to check that all is working properly. You may have to refer to this window when asking for Okolab technical support. Figure 37. Status page 6.2.1.9...

  • Page 29: Touch Screen

    Figure 38. Digital input 6.2.2 Touch Screen Touch Screen Settings are adjusted by following the two steps below (look at the touching finger) Figure 39. Touch screen settings 6.2.2.1 Options In this window you can set the scale of the X axis (time duration) of the graphs displayed in the main page and the buzzer sound frequency.

  • Page 30: Brightness

    6.2.2.2 Brightness This page allows you to increase/reduce the brightness of the touch screen, scroll the bar or press the +/- buttons. Figure 41. How to enter in Alarms page 6.2.2.3 Calibration When pressing the calibration icon, the procedure for the calibration of the touch screen will start. Figure 42.

  • Page 31: Alarms

    6.2.2.5 Alarms This page allows you to set deviation values (from set-point) beyond which you want the system to trigger an Alarm. Figure 44. How to enter in Alarms page Tip ►Flag “Buzzer” if you want the Alarm to be acoustical as well rather than just being displayed. Figure 45.

  • Page 32: Overview

    Glance Mode follow the color coding reported in paragraph 6.1.1. Figure 46 shows an overview of the complete system displaying current values of all controlled parameters. Similarly to the Status Page you may have to refer to this window when asking for Okolab technical support. Figure 46.Overview page Figure 46 shows the Overview page when your system is equipped with the Objective Heater (OBJ COLLAR ####), CO2-O2-UNIT [0-10;...
  • Page 33 Figure 48. Glance Mode with temperature controller, CO2-O2 gas controller and active humidity controller...

  • Page 34: Calibrations

    ALIBRATIONS Carefully read the instructions given in this paragraph and before starting the Chamber Calibration. Note ► It is important to monitor the room temperature during the calibration, as well as reducing air drafts and forced convection. If possible divert any draft coming from the air conditioning system in the room, from the incubation system.

  • Page 35: Objective Heater Manual Calibration

    Tip ► It is recommended using an Okolab SENSOR LID (available for purchase) to secure the Fine Gauge Thermocouple in place in the Reference Well during calibration or operation in Sample Feedback Mode (see Figure 50). Sensor lids are available for the following: 35 mm, 60 mm Petri-dish, chamber slide and chambered cover glass.
  • Page 36 Figure 20 and select “Use Manual” (see Figure 22). Next click on “+” or “-” on the “Offset” tab (see Figure 22) to adjust the Objective Heater offset value. Okolab recommends starting with an offset value corresponding to the difference between the set point temperature and the actual temperature measured by the Fine Gauge Thermocouple.

  • Page 37: Objective Heater Self-Calibration

    Before starting the Objective Heater Self-Calibration carefully read the instructions given in this paragraph and in paragraph 6.2.1.3. The goal of the Self-Calibration procedure is to adjust the Objective Heater temperature by automatically varying its offset; while maintaining the sample at the desired temperature (e.g. 37.0 °C) once the oil immersion objective is in contact with the bottom of the well.
  • Page 38 Enable the Objective Heater as shown in Figure 20, the window in Figure 23 will open. Follow the instructions on the message: first press “CALIBRATE”, next bring the immersion lens in contact with the petri-dish. Following this sequence of instructions is REQUIRED to place the Fine Gauge Thermocouple in standby and act on the Objective Heater only.

  • Page 39: Maintenance

    To polish the Stage Incubator and the Humidifying Module, if it is present, you can use distilled water or alcohol Verify the status of all cables and if some cable is damaged, contact Okolab to receive assistance Before cleaning the unit, pull out the mains plug.

  • Page 40: Support

    UPPORT In order to send directly an email to the Okolab staff, you can use the QR codes shown in the following images: • • For commercial info: For technical info: Figure 51. QR code for commercial info contact Figure 52.QR code for technical info contact Please, do not hesitate to contact Okolab if you need any further commercial information or technical support.
  • Page 41 • Contact our technical support (sibillo@oko-lab.com) by e-mail to take an appointment for the web assistance.

  • Page 42: Troubleshooting

    Incorrect operations are often mistaken for malfunction. If you think that there is something wrong with a component, see the troubleshooting scheme below. If the problem persists even after troubleshooting as described below, please ask for Okolab support Symptom Probable cause...

  • Page 43: Technical Specifications

    11 T ECHNICAL PECIFICATIONS H301-T-UNIT-BL-PLUS – Technical Specifications Chamber Temperature Range: 25-60°C Temperature Sample Temperature range: 3°C above ambient temperature to 60°C Step size: 0.1°C Accuracy:0.1°C in Sample Mode, 0.3°C in Chamber Mode if room temperature remains within ± 1°C...
  • Page 44 Figure 4. OBJ-COLLAR Connection (Optional) ..................12 Figure 5. OKO-TOUCH Connection ...................... 13 Figure 6. CO2-O2 UNIT-BL [0-10; 1-18] and H301-T-UNIT-BL-PLUS ............... 14 Figure 7. Homepage of the Control Unit Touch Screen Display..............15 Figure 8. How to change the Temperature set point ................16 Figure 9.

  • Page 45: Revision Table

    Okolab S.r.l. accepts no responsibility for damage due to unsatisfactory packing. Upon examination of Okolab S.r.l., if the unit is found to be defective, it will be repaired or replaced at no charge. This warranty does not apply to defects resulting from any actions of the purchaser. Okolab S.r.l.

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