1. Manuals
  2. Brands
  3. Metertech Manuals
  4. Measuring Instruments
  5. SP-8001
  6. User manual

Metertech SP-8001 User Manual

Uv-vis spectrophotometer
Hide thumbs Also See for SP-8001:
Table of Contents

Advertisement

Quick Links

Download this manual
SP-8001 UV-VIS Spectrophotometer
USER'S MANUAL
Metertech Inc.
Version 1.06

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the SP-8001 and is the answer not in the manual?

Questions and answers

Related Manuals for Metertech SP-8001

Summary of Contents for Metertech SP-8001

  • Page 1 SP-8001 UV-VIS Spectrophotometer USER’S MANUAL Metertech Inc. Version 1.06...
  • Page 3 Metertech Inc. provides this publication “as is” without warranty of any kind, either express or implied, including, but not limited to the implied warranties of merchantability or fitness for a particular purpose. All rights are reserved, including those to reproduce this publication or parts thereof in any form without permission in writing from Metertech Inc.

  • Page 4: Table Of Contents

    CONTENTS Chapter 1 INTRODUCTION ----------------------------------------------------------------- 04 §1-1 Specification ------------------------------------------------------------------------ 04 §1-2 Safety Notes ------------------------------------------------------------------------- 05 Chapter 2 INSTALLATION -------------------------------------------------------------------- 06 §2-1 Unpacking --------------------------------------------------------------------------- 06 §2-2 Installation --------------------------------------------------------------------------- 07 Chapter 3 GENERAL --------------------------------------------------------------------------- 08 §3-1 User Interface ------------------------------------------------------------------------ 08 §3-2 General Function Description ----------------------------------------------------- 10 §3-3 Initialization ------------------------------------------------------------------------- 12 Chapter 4...
  • Page 5 Appendix A Thermal Controller Operation Instruction ----------------------------------- 51 Appendix B Sipper Operation Instruction -------------------------------------------------- 53...

  • Page 6: Chapter 1 Introduction

    In order to obtain maximum performance and trouble-free operation with the SP-8001, It is recommended that you carefully read this manual before using the SP-8001, and thereafter keep it close at hand for future reference.

  • Page 7: Safety Notes

    §1-2 Safety Notes Although this SP-8001 UV-VIS Spectrophotometer is user friendly, the instrument and its accessories described in this manual are designed to be used by properly trained persons only. READ THIS SECTION CAREFULLY BEFORE INSTALLING AND USING THE INSTRUMENT AND ITS ACCESSORIES.

  • Page 8: Chapter 2 Installation

    §2-1 Unpacking The SP-8001 (Figure 2.1 and Figure 2.2) should be delivered with intact packaging that contains one carton with items listed in the packing list. Unpack the carton and inspect carefully. If any part is damaged or missing, contact your local dealer immediately.

  • Page 9: Installation

    ♦ near X-ray equipment ♦ near centrifuge B. Check and set the voltage. The SP-8001 can be performed with selective power supply at 100, 120, 220, or 240Vac 50/60Hz from a properly wired and grounded single phase power outlet. Check the voltage shown in the frame above power inlet. If it is not the desired, use flat screw driver to open the protective plastic cover.

  • Page 10: Chapter 3 General

    Chapter 3 GENERAL The SP-8001 is designed to be a self-contained instrument and can work as a standalone machine or can work with other peripherals together. The SP-8001 has one membrane-type keyboard and a 320X240 LCD display as the user interface.
  • Page 11 screen. QUICK RUN To pop up a Parameter File List (Figure 3.2) for users to select operation settings stored in memory. In this file list, users can view all parameter settings in different operation modes that is distinguished by the first 2 letters in the first column.

  • Page 12: General Function Description

    §3-2 General Function Description Functions being used in the SP-8001 are listed in the corresponding modes and are shown in structured maps. For those functions being widely used in most of the modes will be described below. Load Param For loading existing parameter settings stored in memory.
  • Page 13 12:00:00 500.0nm 0.000A >>> File Name:ART Press UP DOWN key to select... Figure 3.4 Filename input screen Print Data To print data or graph from the current screen. Sample Control To be functioning only when certain accessory being used. Next Screen Accept the current settings and switch into the measuring display screen.

  • Page 14: Initialization

    §3-3 Initialization Turn on the power of the SP-8001, a start-up screen (Figure 3.5) shows up and the initialization of system will be automatically done. The version of software coming up with the instrument is also shown in the start-up screen. User-installed accessory will be automatically detected during the initialization.
  • Page 15 Two functions, Quick Run and Sample Control, are available in this screen. Quick Run is a function the same as described before by using the QUICKRUN key. Sample Control will be functioning only when the multi-cell holder or the sipper is used.

  • Page 16: Chapter 4 System Setup

    Chapter 4 SYSTEM SETUP In System Main Menu, selecting item 6 will get into the System Setup Menu screen (Figure 4.1). In this screen, users are allowed to alter several parameters described below. In this screen only one function, Load B-Line, is available. 12:00:00 500.0nm 0.000A...
  • Page 17 Setup Lamp Use function keys to set up parameters for visible and UV lamps (Figure 4.3). Switch UV is for switching ON/OFF of UV lamp. Reset Vis Hr is for resetting the usage time of the tungsten lamp. Reset UV Hr is for resetting the usage time of the UV lamp. Energy Vis is for measuring the power of the tungsten lamp.
  • Page 18 12:00:00 500.0nm 0.000A Serial Port Parameter Setup 1.Baud rate :38400 2.Bit length 3.Parity check :NONE 4.Stop bit 5.Remote status :OFF Press < > key to select... Print Prev Data Screen Figure 4.5 Serial Port Parameter Setup screen Setup Hardware In this option, three adjustable items are shown (Figure 4.6). Use arrow keys to move cursor to proper position and proceed as instructed at the bottom of this screen.
  • Page 19 12:00:00 BANDWIDTH 676.0nm 0.000A 100.0 50.0 676 nm λ1=655.59nm λ2=657.44nm Bandwidth=1.85nm λmax=656.12nm Measurement completed... Print Prev Data Screen Figure 4.7 Bandwidth measurement screen used for calibration (Figure 4.7). If the calibration for this instrument is correct, there is a peak shown around wavelength 656.1nm and the bandwidth is less than 2nm.

  • Page 20: Chapter 5 Photometric

    Chapter 5 PHOTOMETRIC This is a simple measurement mode with fixed wavelengths. According to the parameter setup, users can repeat a measurement and a table of the measurement results will be shown. The results can be printed with proper settings. In System Main Menu, selecting item 1 will get into this mode.

  • Page 21: Photometric Function Structure

    For functions not described below, please refer to General function description section. §5-3 Basic Photometric Measurement The following is a description of the basic procedure for carrying out measurement in the SP-8001 Photometric measurement mode. For example, five samples are ready to proceed photometric measurement in 4 wavelengths: 400, 450, 500, and 550.
  • Page 22 5) with proper entries as instructed. If any setting needs correction, use up/down arrow keys to move the cursor to the proper position. When all is done, press F5 Next Screen to next step. Step 3. Get a sample ready and press READ. The reading of that sample will be displayed on the screen.
  • Page 23 Step 1. In System Main Menu, press λ in the keyboard to get into the Lambda screen (Figure 5.4). Step 2. Get the sample ready for measurement. In the Lambda screen, key in the value for wavelength and press ENTER. The reading of the measurement will show up in the screen. Step 3.
  • Page 24 data will be displayed at the bottom of the screen (Figure 5.7). Up to 60 sets of data can be saved. 12:00:00 LAMBDA 500.0nm 0.000A λ : 500.0 nm Data: 0.000 Data01 500.0nm 0.000A Press λ & keyin wavelength... View Save Mode Sample...

  • Page 25: Chapter 6 Spectrum

    Chapter 6 SPECTRUM This is the mode in which spectral measurement is performed. Measured spectra can be saved to memory and be subjected to various data manipulation described in details below. The measured spectra can be zoomed by changing the interested range. Interested point of the spectra can be tracked manually and the corresponding data will be shown in the screen.

  • Page 26: Spectrum Function Structure

    2nm greater than the Start Wavelength. Measure Mode Selecting measurement results in ABS as for absorbance or in %T as for transmittance. Low Value For entering the lower limit of measurement result between –0.3 and 2.99 for ABS and between 0 and 119.9 for %T, respectively. High Value entering upper...

  • Page 27: Basic Spectrum Measurement

    §6-3 Basic Spectrum Measurement The following is a description of the basic procedure for carrying out measurement in the SP-8001 Spectrum measurement mode. For example, a spectrum of sample is to be measured from 400nm to 800nm and the results should be bounded between 0 and 1.5Abs. Scanning speed is set at 1000nm/min.
  • Page 28 12:00:00 SPECTRUM 500.0nm 0.000A Parameter Setup 1.Start wavelength(nm) :400.0 2.Stop wavelength(nm) :800.0 3.Measure mode :ABS 4.Low value(ABS) :0.00 5.High value(ABS) :1.50 6.Scan speed(nm/min) :1000 7.Overlay screen :Yes Number from 190 to 1098... Load Save Print Sample Next Param Param Data Control Screen Figure 6.2 Setting of parameters arrow keys to move the cursor to the proper position.
  • Page 29 12:00:00 SPECTRUM 800.0nm 0.132A 1.50 0.75 0.00 400 nm Press READ when ready... Smooth Zoom Show Peak Origi- Data Data Track Valley Figure 6.4 Functions for manipulating graph Step 6. If users want to view part of the graph, function Zoom Data is designed for this purpose. Press F2 Zoom Data, then cursor will sequentially move through 1.50 (High Value), 0.00 (Low Value) of the vertical axis and 400 (Start Wavelength), 800 (Stop Wavelength) of the horizontal axis in the graph.
  • Page 30 12:00:00 SPECTRUM 533.2nm 0.762A 1.50 0.75 0.00 400 nm Press < > key to move... View Save Print Data Data Data Figure 6.6 Graph under Show Track function point will be displayed at the up right corner in the screen. Step 8.
  • Page 31 12:00:00 SPECTRUM 800.0nm 0.132A 1.50 0.75 0.00 400 nm Press hot key to processing... View Search Search Less More Data Peak Valley Point Point Figure 6.8 Searching peaks of the spectrum data of those points can be viewed by pressing F1 View Data to see the wavelength and the corresponding reading (Figure 6.9).

  • Page 32: Chapter 7 Timescan

    Chapter 7 TIMESCAN This is the mode in which absorbance versus time measurement of a sample is performed for the accurate study of continuous changes in reaction rates. Delay Time can be set before the start of Scan Time. Results can be shown in a graph and also be manipulated in various ways as needed. In System Main Menu, selecting item 3 will get into this mode.

  • Page 33: Timescan Function Structure

    %T as for transmittance. Low Value For entering the lower limit of measurement result between –0.3 and 2.99 for ABS and between 0 and 119.9 for %T, respectively. High Value entering upper limit measurement result between –0.29 and 3.00 for ABS and between 0.1 and 120.0 for %T, respectively.

  • Page 34: Basic Timescan Measurement

    §7-3 Basic TimeScan Measurement The following is a description of the basic procedure for carrying out measurement in the SP-8001 TimeScan measurement mode. For example, users want to know the measurement change of a sample in a time span of 300 seconds at wavelength 500nm.
  • Page 35 Step 3. Make the sample ready and press READ. The measurement result will be displayed in the TimeScan screen (Figure 7.3) in real-time mode. 12:00:00 TIMESCAN 500.0nm 0.000A 101.0 100.0 99.0 0 sec Press READ when ready... Access Mani- Clear Prev Data pulate...

  • Page 36: Chapter 8 Kinetic

    Chapter 8 KINETIC In this mode, the time-dependent changes in absorbance (ABS) at a fixed wavelength are measured and recorded. A typical application in this mode is the measurement of an enzyme activity. The time changes in absorbance will be displayed on screen and the absorbance can be found at each sampling interval, set according to Lag Time plus with Rate Time.
  • Page 37 Kinetic mode. This number of data is used for the activity calculations. The value should range from 2 to 20. Lag Time (sec) For setting the time in second from the start of measurement during which data will not be acquired for activity calculations. The value should range from 2 to 9999.

  • Page 38: Kinetic Function Structure

    §8-2 Kinetic Function Structure Kinetic Load Param Save Param Change Scale Print Data Repeat Measure Sample Control Print Data Next Screen Data List Sample Control Prev Screen Manipulate Linear Fit Clear Graph Show Track Print Data Print Data Original Prev Screen Data List Switch to the KINETIC Data File List screen for users to review data obtained in previous measurements after setup of...

  • Page 39: Basic Kinetic Measurement

    §8-3 Basic Kinetic Measurement The following is a description of the basic procedure for carrying out measurement in the SP-8001 Kinetic measurement mode. For example, users want to know the reaction of a reagent in a time span of 60 seconds after 60-second lag time by sampling 5 times at wavelength 405nm, assuming that factor is 3253 and unit is set as U/L.
  • Page 40 12:00:00 KINETIC 405.0nm 0.540A 1.00 0.35 -0.30 ∆T=60.0 Factor=3253.000 ∆ABS/∆T=0.11308( ABS/min) ∆C /∆T=367.86414( U/L/min) =0.00188T + 0.27194 Press READ when ready… Data Mani- Clear Print Prev List pulate Graph Data Screen Figure 8.3 Kinetic measuring screen 12:00:00 KINETIC 405.0nm 0.540A 1.00 0.35 -0.30...

  • Page 41: Chapter 9 Quantitative

    Chapter 9 QUANTITATIVE This is the mode in which the concentration of an unknown sample under a specific wavelength can be determined by creating a calibration curve from standard samples. Four methods for curve fitting can be selected: straight line with or without passing through origin and 2 order polynomial with or without passing through origin.

  • Page 42: Quantitative Function Structure

    Have Unit (Yes/No) Selecting the unit option for measurement results. When Yes is selected, pick the right unit out of the thirteen build-in units in table. Method Four methods of data fitting are available: 1’ord.(Origin) for straight line through origin, 1’ord.(No Org) for straight line not passing through origin, 2’ord.(Origin) for 2 ordered polynomial through origin, and 2’ord.(No Org) for 2...

  • Page 43: Basic Quantitative Measurement

    For example, there are three standard samples with known concentration and absorbance. If the absorbance values are unknown, users can also use the SP-8001 to find out the corresponding absorbance values. Using those data, the relationship between concentration and absorbance can be build up through curve fitting technique.
  • Page 44 Step 5. In the Quantitative Measure Sample screen (Figure 9.5), get the unknown sample ready and press READ, then the SP-8001 will measure the absorbance of the sample and calculate the corresponding concentration by using the equation obtained before. Results will be shown in the screen and can also be printed by pressing F3 Print Data.
  • Page 45 12:00:00 QUANTITATIVE 500.0nm 2.325A Measure Sample -------------------------------------- Sample No. Conc.( G/L) 0.521 5.2100 1.137 11.370 2.325 23.250 Press READ when ready... Print Sample Prev Data Control Screen Figure 9.5 Quantitative Measure Sample screen K1 and K0 are the coefficients in the equation and depend on the selected method of curve fitting.

  • Page 46: Chapter 10 Accessories

    Single cell holder is a standard accessory. When an optional accessory is to be used, users should turn off the instrument first and then do the hardware installation as well as the cable connection. The SP-8001 will automatically detect attached accessory. When the instrument is turned on, the detected accessories will be shown during initialization.
  • Page 47 A Multi-Cell Parameter Setup screen (Figure 10.2) will show up when function Sample Control being pressed. Detailed settings and respective functions will be described below. 12:00:00 500.0nm 0.000A Multi-Cell Parameter Setup 1.Driver cell Number(1-6) 2.Reagent blank corr.(cell 1) :Yes Cell from 1 to 6... Cell=6 Cell Cell...

  • Page 48: Sipper

    §10-2 Sipper The Sipper (Figure 10.3) is an optional accessory that enables samples to be drawn into a flow cell for automatic measurement. After the measurement is complete the sample may be sent to a waste bottle or returned to its original vessel. From cell To cell 25-pin male connector...
  • Page 49 only applies on the sipping operation for Sipper Time. Pre Air Purge Time (sec) For entering the value of time during which Sipper will do the air purge operation for the previous sample. The value should range from 0 to 99.9. Pre Sipper Time (sec) For entering the value of time during which Sipper will draw the residual of previous sample passing through the flow cell.

  • Page 50: Chapter 11 Maintenance

    Chapter 11 MAINTENANCE The information given in this section deals only with those parts of maintenance or service which can be safely carried out by users. Work other than those detailed later should be carried out by a trained service engineer. Poor instrument performance or failure can usually be attributed to simple failure of the tungsten lamp.

  • Page 51: Replacement Of Lamps

    §11-2 Replacement of Lamps This instrument is designed for easy access in lamp replacement. Minimum adjustment is required for the replacement. The tungsten halogen lamp is seated in a specially designed holder that is located in the right half part in the lamp compartment (Figure 11.1). However the deuterium lamp that comes up with a ring-typed aluminum seat is located in the left half part respectively.
  • Page 52 the deuterium lamp. Pull the deuterium lamp straightly upward to remove. ♦ Put a new deuterium lamp back to the holder and screw it tightly. ♦ Reconnect the rapid connector of the deuterium lamp. ♦ Remount the cover of lamp compartment. WARNING: For replacing deuterium lamp or halogen lamp, be sure to contact your local agent, and use vendor’s designated light sources.
  • Page 53 Appendix A THERMAL CONTROLLER OPERATION §A-1. Installation of thermal controller on SP8001 machine -Power off SP8001 machine. -The thermal controller can be applied to SP8001 in two approaches. It can be an independent unit for rectangular cuvette, or accompanied with sipper for flow through cell.
  • Page 54 -The thermal controller is designed to adapt for two types of flow cell; aperture height with 8.5 mm and 15 mm. The default height is 8.5 mm. If 15 mm height is needed, please remove an aluminum block right down the bottom surface of cell holder with a cross type screw driver.
  • Page 55 Appendix B SIPPER OPERATION §B-1. Installation of sipper on SP8001 machine -Power off SP8001 machine. -Remove the front panel of cell compartment of SP8001 by loosing two M3 screws behind it. -A sipper assembly as shown on fig. 1 includes one pumping drive, one pinch valve(one sample input, and another air input) two connectors( small one for connecting to electronically thermal controlled cell assembly, large one for connecting to SP8001 machine), two flexible tubes, and front panel.
  • Page 56 pump speed selection for sampling solution at low , medium or high speed. #2 and #4 are for air purge times setting, the pump is always preset at high speed. If you do not need air purge between two samplings, just key in zero value( it is suggested to have air purge on during operation to remove the possible accumulated air bubble inside the cell).
  • Page 57 flow cell& tubes peristaltic tube pinch valve button stop 9-pin(small) connector screw hole sample inlet 24-pin(large) connector sample outlet pumping wheel thermal water inlet&outlet Figure 1. Sipper Assembly(with flow cell connected)

Table of Contents