Shimadzu CBM-20A Instruction Manual
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Shimadzu CBM-20A Instruction Manual

Shimadzu CBM-20A Instruction Manual

System controller for shimadzu high performance liquid chromatograph
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Table of Contents

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FOR SHIMADZU HIGH PERFORMANCE
INSTRUCTION MANUAL (detailed manual)
Read the instruction manual thoroughly before you use the product.
Keep this instruction manual for future reference.
SYSTEM CONTROLLER
LIQUID CHROMATOGRAPH
CBM-20A/20Alite
228-90005T
Jul. 2015

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  • Page 1 228-90005T Jul. 2015 SYSTEM CONTROLLER FOR SHIMADZU HIGH PERFORMANCE LIQUID CHROMATOGRAPH CBM-20A/20Alite INSTRUCTION MANUAL (detailed manual) Read the instruction manual thoroughly before you use the product. Keep this instruction manual for future reference.
  • Page 2 This page is intentionally left blank.

  • Page 3: Cbm-20A Instruction Manual (Brief Manual

    (such as SIL-20A and SIL-20AC), but with some of their functionality or specifications modified. However, unless an exception is specifically indicated, the CBM-20A/20Alite Instruction Manual will refer to them by the model name of their respective normal versions. For example, models SIL-20A, SIL-20AHT, and SIL- 20AHT UFLC are collectively referred to as model SIL-20A.

  • Page 4: Introduction

    Shimadzu representative to request a replacement. • To ensure safe operation, read all Safety Instructions before using the product. • To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, or re-installation (after the product is moved) is required.

  • Page 5: Warranty And After-Sales Service

    Limitation of Liability: 1) In no event will Shimadzu be liable for any lost revenue, profit or data, or for special, indirect, consequential, incidental or punitive damages, however caused regardless of the theory of liability, arising out of or related to the use of or inability to use the product, even if Shimadzu has been advised of the possibility of such damage.
  • Page 6 "7 Hardware Validation" P. 7-1 Hardware Validation This is a contract under which a qualified Shimadzu-approved engineer performs periodic component and system validation, and provides reports of Contract the results. Details of the contract can be obtained from your Shimadzu representative. CBM-20A/20Alite...

  • Page 7: Safety Instructions

     Application Precautions WARNING This instrument is system controller for use with a high performance liquid chromatography system. Use this instrument ONLY for the intended purpose. Using this instrument for any other purpose could cause accidents. CBM-20A/20Alite...

  • Page 8: Installation Site Precautions

    CAUTION • The weight of the CBM-20A is 5.5 kg. During installation, consider the entire weight combined with other LC components. The lab table on which this instrument is installed should be strong enough to support the total weight of the LC system.
  • Page 9  Installation Precautions To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, or re- installation (after the product is moved) is required. WARNING • Take measures to prevent the instrument from falling in the event of an earthquake or other disaster.
  • Page 10 The cord could be damaged, resulting in fire, electrical shock or malfunction. If the cord becomes damaged, contact your Shimadzu representative immediately. • Do not modify the cord in any way. Do not bend it excessively or pull on it.

  • Page 11: Operation Precautions

    • Be careful not to apply water to office equipment such as the PC as well as the instrument. • Install the low-pressure Hg (Mercury) lamp before turning on the power of the lamp. Looking straight at the lamplight could damage eyes. CBM-20A/20Alite...

  • Page 12: Precautions For Instrument Inspection, Maintenance, Adjustment And Care

    • Never remove the main cover. This may cause injury or malfunction of the instrument. The main cover does not need to be removed for routine maintenance, inspection and adjustment. Have your Shimadzu representative perform any repairs requiring removal of the main cover.

  • Page 13: Static Electricity Precautions

    Air bubbles in liquid facilitate generation of static electricity. Insulated container of spark polyethylene, etc. Dry air Flammable gas present in container. Flammable organic solvent with spark large electrostatic charge. Floor covered with rubber, etc., cannot conduct electricity away. CBM-20A/20Alite...

  • Page 14: Preventing Static Electricity Accidents

    Connect clip to metal parts. Connect to ground terminal or other grounding point of the Reduce the opening instrument to ground metal with a cap. container. 18 Liter metal can (preferably plated) Liquid's static will be conducted through container to ground. CBM-20A/20Alite...
  • Page 15 Use tubing with an inner diameter of at least 2 mm for drain lines with high flow rates. CAUTION • Periodically check the tubing connections for leaks. Air bubbles in liquid can multiply the electrostatic charge by a factor of 20, 30 or more. CBM-20A/20Alite XIII...
  • Page 16 • Use as small a container as possible to minimize damage in the event of fire. • Keep the room at a proper humidity. Ambient humidity exceeding 65 % will prevent static. For Reference Anti-static equipment (anti-static clothing, shoes and matting) and charge measurement equipment (potentiometer) are sold by specialty manufacturers. CBM-20A/20Alite...

  • Page 17: In Case Of Emergency

    Procedure Turn the power to the instrument OFF. Disconnect the power cable at the rear of the instrument. When the instrument is used again, inspect the instrument and, if necessary, contact your Shimadzu representative to request servicing.  In Case of Power Outage CAUTION Take the following measures in the event of a power outage.

  • Page 18: Maintenance, Inspections, And Adjustment

    Instruction Manual. • Periodic inspection/calibration should be requested to your Shimadzu representative. • Replacement cycles described for periodic replacement parts are rough estimate. • Replacement may be required earlier than the described replacement cycles depending on usage environment and frequency.

  • Page 19: Precautions For Mobile Phase Selection And Use

    • Always degas the mobile phase, as air bubbles may tend to form during solvent mixing or during temperature or pressure changes. Air bubbles may cause pump malfunctions and detector signal noise. • Understand the properties, including boiling points, firing point and viscosities, of the mobile phase. "9.8 Mobile Phase Characteristics" P. 9-56. CBM-20A/20Alite XVII...
  • Page 20 Introduction This page is intentionally left blank. XVIII CBM-20A/20Alite...

  • Page 21: Table Of Contents

    Contents Instruction Manuals..................... I  CBM-20A Instruction Manual (brief manual) ............I  CBM-20A Instruction Manual (detailed manual) [PDF Instruction Manual] ..... I  Model Names ......................I Introduction ........................ II Warranty and After-Sales Service................III Safety Instructions ....................V ...
  • Page 22 CBM-20A ......................2-2 Back ......................2-3  CBM-20A ......................2-3  CBM-20Alite ......................2-4 Optional Parts....................2-5  Back View of Optional Parts for CBM-20A ............2-5 Applications and Windows ................2-6  List of Windows ....................2-8 "Group Monitor" Application .................2-9 2.5.1 "HPLC Explorer" Section................2-10 2.5.2 "Status Summary"...
  • Page 23 Overall Flow of Operation ................4-2 4.1.1 Starting the System Components .............. 4-3 4.1.2 Starting Internet Explorer and Connecting to the CBM-20A ...... 4-3 4.1.3 Logging In and Displaying the "Analysis" Tab Page ........4-3 4.1.4 Selecting and Editing a Method File ............4-4 4.1.5...
  • Page 24 Parameters for the Autosampler .............. 4-16 4.2.5 Parameters for the Column Oven ............4-17 4.2.6 Parameters for the Detector..............4-19 4.2.7 Parameters for the CBM-20A..............4-20 Creating Sequence Files ................4-22 4.3.1 Selecting Sequence Files................. 4-22 4.3.2 Table and Buttons Used for Analysis Sequences ........4-24 4.3.3...
  • Page 25 Saving All Component Parameters to a PC ..........5-33 5.8.2 Downloading All Component Parameters from a PC ....... 5-35 5.8.3 Initializing Parameters................5-37 Managing Groups ..................5-39 5.9.1 Displaying the "Group Settings" Application Window....... 5-39 5.9.2 "Group Information" Tab Page ..............5-41 CBM-20A/20Alite Contents-5...
  • Page 26  START Command ...................5-143  Completing the Program Setting ..............5-143  Gradient Curve ....................5-143 5.13.8 Fast LC Mode..................5-145  Fast LC Mode Settings ..................5-145  Restrictions of Fast LC Mode ................5-145  Using an SPD-M20A/M30A Detector ...............5-146 Contents-6 CBM-20A/20Alite...
  • Page 27 7.3.1 Environment....................7-4 7.3.2 Installation Site................... 7-4 Equipment Required for Validation ...............7-6  Testing Equipment ....................7-6  Standard Reagents for Validation ............... 7-7  Hardware Testing Supplies ................. 7-8 Validation: System Controller ...............7-9 7.5.1 Check Terms....................7-9 CBM-20A/20Alite Contents-7...
  • Page 28 Checking Maintenance Information..............8-9 8.2.1 Displaying the "Maintenance" Tab Page............ 8-9 8.2.2 "Maintenance" Tab Page Display............... 8-9 8.2.3 List of Displayed Maintenance Items ............8-10 Periodic Inspection and Maintenance ............8-13 8.3.1 Periodic Inspection and Maintenance ............8-13 8.3.2 Exterior Cleaning..................8-13 Contents-8 CBM-20A/20Alite...
  • Page 29  Grounding ......................9-6 Connecting the System Controller and Components........9-7 9.2.1 Types of Connector..................9-7  CBM-20A ......................9-7 9.2.2 Connecting the Optical Cable ..............9-13 9.2.3 Installing the CBM-20Alite in an Autosampler or Solvent Delivery Module ..............9-14 ...
  • Page 30  Group Management ...................9-42  User Management .....................9-42  Managing Users as a Group ................9-42 Specifications .....................9-43 9.5.1 CBM-20A....................9-43 9.5.2 Specifications for External Connections........... 9-43  Input/Output Connectors ..................9-43  Connectable HPLC Components ...............9-44 9.5.3 Control Specifications ................9-48 ...
  • Page 31 Action in the Event of an IP Address Allocation Error ......Appendix-11  DHCP Packets ..................Appendix-12  System Name and Host Name ............. Appendix-14  Name Solution Using NetBIOS over TCP/IP ........Appendix-15  Character Types and Name Length ............. Appendix-15 Index CBM-20A/20Alite Contents-11...
  • Page 32 Contents This page is intentionally left blank. Contents-12 CBM-20A/20Alite...

  • Page 33: Chapter 1 Configuration

    Configuration Contents Overview ......................1-2 Component Parts .................... 1-5 Optional Parts ....................1-6...

  • Page 34: Overview

    1. Configuration Overview The CBM-20A/CBM-20Alite is a system controller that connects to and controls the components in Shimadzu's LC-30A/20A/10Avp/10A-series High-performance Liquid Chromatograph. It can be used for a number of different purposes, from centralized control to fully automated operation of liquid chromatography systems with various component configurations.

  • Page 35: Equipment Required For Connecting To The Network

    Web browser. The system controller can also be connected to a Shimadzu workstation using the TCP/IP protocol. The hardware requirements for using the Web browser and connecting to the network are described below.
  • Page 36 1. Configuration • Use commercial products satisfying the specifications given in the remark column. • One LAN cable is required for every PC, CBM-20A/20Alite, and SPD-M20A/M30A. • If the total number of PCs, CBM-20A/20Alite System Controllers, and SPD-M20A/M30A Photodiode Array Detectors is greater than the number of ports on the switching hub, increase the number of ports as appropriate using cascade connections.

  • Page 37: Component Parts

    These 2-digit figures refer to the last two digits in the product number of the instrument. -31, -41 is for products destined for Japan, while -32, -42 and -38, -48, -58 are for use in other countries.  CBM-20A (S228-45012) Part Part No. Q’ty...

  • Page 38: Optional Parts

    1. Configuration Optional Parts Optional units available for this instrument are listed below. For information about other optional units not listed below, contact your Shimadzu representative  Optional Boards Part Part No. Remark CBM-OPT4 Optical-connector S228-45652-48 For optical-connector expansion (CBM-20A only)

  • Page 39: Rs-232C Cables

    Chromatopac other than C-R8A 9-pin to 25-pin crossover cable for connecting RS-232C cable, 5 m S228-35398-95 to Chromatopac other than C-R8A 9-pin to 25-pin crossover cable for connecting RS-232C cable, 10 m S228-35398-90 to Chromatopac other than C-R8A CBM-20A/20Alite...
  • Page 40 1. Configuration This page is intentionally left blank. CBM-20A/20Alite...
  • Page 41 Parts Identification and Function This chapter provides details on the names and functions of the various parts on the front and back of the system controller and also describes commonly used windows. Contents Front Cover ..................... 2-2 Back ........................ 2-3 Optional Parts ....................

  • Page 42: Front Cover

    2. Parts Identification and Function Front Cover  CBM-20A Run indicator Connection indicator Status indicator Power switch Press in Press again (power ON) (power OFF) Fig. 2.1 Name Description Run indicator Turns ON when analysis starts and turns OFF when analysis stops.

  • Page 43: Back

    AC output connectors linked to the power switch. They can be used to supply power to (AC OUT) Shimadzu HPLCs (SIL-10A Series, FRC-10A, Sub-controllers). Do not use them for any other application. Display the status of connection to the network using LED indicators.

  • Page 44: Cbm-20Alite

    100M : Turns ON when operating at 100 Mbps. (100M/ACT/LINK) ACT : Turns ON when exchanging data. LINK : Turns ON when linked to the network. Ethernet connector Connector for connecting to the network. (ETHERNET) Initialization button (INIT) Push to initialize the system controller or clear errors. CBM-20A/20Alite...

  • Page 45: Optional Parts

    2.3 Optional Parts Optional Parts  Back View of Optional Parts for CBM-20A Optical-connector Expansion Board (CBM-OPT4) A/D Board (PC-55N) Fig. 2.4 Name Description Optical-connector Expansion Board Board for optical-connector expansion. (CBM-OPT4) Board for analog-digital conversion for connecting a detector that A/D Board (PC-55N) uses the analog output.

  • Page 46: Applications And Windows

    "Login" and "Error" windows that are displayed when operating the system controller are described in the following section. Refer to "8.1 Checking the System" P. 8-2 for the system check aplication.Refer to "5.9 Managing Groups" P. 5-39 for the group management. CBM-20A/20Alite...
  • Page 47 2.4 Applications and Windows "Group Monitor" application P.2-9 "Group Monitor" application P.2-9 "Login" window P.2-16 "Login" window P.2-16 "System Check" application "Group Settings" application P.8-2 P.5-39 "Analysis Execution" application P.2-17 CBM-20A/20Alite...

  • Page 48: List Of Windows

    "Gradient Curve" window, "Load File" window, "Save File" window "Advanced Shutdown Settings" window, "Startup "Queue" tab page Advanced Settings" Window "System Check" application "Error" window "Group Settings" application "Group Information" tab page "System Administration" tab page "Advanced Settings" window "User Management" tab page CBM-20A/20Alite...

  • Page 49: Group Monitor" Application

    "Group Monitor" Application This is the first application that is started when the Web server is accessed. The Group Monitor application window consists of three sections: "HPLC Explorer", "Status Summary", and "System Monitor". HPLC Explorer Status Summary System Monitor CBM-20A/20Alite...

  • Page 50: Hplc Explorer" Section

    These icons are used to display other groups in a tree Name of other group format. Click on an "other group" icon or a group name to change the information displayed in the "HPLC Explorer" section and "Status Summary" section to the information for that group. 2-10 CBM-20A/20Alite...

  • Page 51: Status Summary" Section

    If a CMD is not connected to the column oven, the comments for the current analysis method are displayed. CBM-20A/20Alite 2-11...

  • Page 52: System Check" Tab Page

    "Login" window for the "System Check Execution and Result" window. Last Date Displays the date and time of the last system check. Result Displays the result of the last system check. User Performed Check The user name that performed the last system check. 2-12 CBM-20A/20Alite...

  • Page 53: Maintenance" Tab Page

    Displays the model names of the connected components. Item Displays maintenance items. Result Displays results related to the recommended replacement values. Present/ Displays the present values and the recommended replacement Recommendation values. Displays the dates when the corresponding parts were last Date Last Replaced replaced. CBM-20A/20Alite 2-13...

  • Page 54: System Monitor" Section

    (repeat injection) number of injections, injection volume, name of the method file, and run time. Displays the set values for the LC system components, such as Monitor solvent delivery modules, autosamplers, column ovens, and detectors. 2-14 CBM-20A/20Alite...
  • Page 55 Home : Return to the standard size When the cursor is moved outside the chromatogram or when the "Analysis Execution" application is logged in, these operations become ineffective. These operations are effective for the chromatogram of the detector. CBM-20A/20Alite 2-15...

  • Page 56: Login" Window

    Click to login to the "Analysis Execution" application. Click to login to the system controller's "Group Settings" application. "5.9 Managing Groups" P. 5-39 Click to close the "Login" window without logging in. Click to change the password. "5.10 Changing Passwords" P. 5-55 2-16 CBM-20A/20Alite...

  • Page 57: Analysis Execution" Application

    "4.2 Creating Method Files" P. 4-10 Click to display the "Queue" tab page. [Queue] tab "5.4 Analysis Using Queues" P. 5-10, "5.5 Shutdown and Startup Settings" P. 5-14 Click to display the "Print" window. Print button "4.6 Printing Files" P. 4-36 CBM-20A/20Alite 2-17...

  • Page 58: Analysis" Tab Page

    This page is used to start the analysis sequence, edit the selected method or sequence files, control the system components, and display chromatograms. The "Analysis" tab page consists of three sections: "Sequence", "Method", and "Monitor". "Sequence" section "Method" section "Monitor" section 2-18 CBM-20A/20Alite...

  • Page 59: Sequence" Section

    Click again to Pause button resume analysis.  Status Display The system's status is displayed in the [Status Display] field. "Sequence" section "Status" tab Description display page display Ready Ready Analysis can be performed. CBM-20A/20Alite 2-19...
  • Page 60 The column oven door is open. StackOpen Not Ready The rack changer stack is open. InitializingChanger Not Ready The rack changer is initializing. WaitSIL Not Ready The autosampler is not ready. DetectingRack Not Ready The changer is performing rack detection. 2-20 CBM-20A/20Alite...

  • Page 61: Method" Section

    If there are components that are not connected to the system controller, the buttons and display/settings items for those components are displayed in gray. Direct-control buttons Basic settings Component icons Display item Description Current Method Displays the name of the current method file. CBM-20A/20Alite 2-21...
  • Page 62 Cancels zero calibration on the optional A/D boards. (free button) Previous-vial Used to return to the fraction collector's previous vial. button Next-vial button Used to advance to the fraction collector's next vial. Fraction-collector Turns fraction collector operation ON/OFF. button 2-22 CBM-20A/20Alite...
  • Page 63 Select the detector from the pull-down menu. Basic detector Set the wavelength. settings The data value is displayed. (The item that can be set and displayed vary with the detector type.) Basic fraction- The vial number undergoing fractionation is displayed. collector settings CBM-20A/20Alite 2-23...
  • Page 64 "30A" or "20A" models.) Click to display the "Mobile Phase Reserve Volume Setting" window. The Mobile-phase mobile phase name and reserve volume can be set from this window. icon "5.2 Setting the Mobile Phase Reserve Volume" P. 5-5 2-24 CBM-20A/20Alite...

  • Page 65: Monitor" Section

    The method parameters and configuration parameters for the system System- controller can be set from this page. controller icon "4.2.7 Parameters for the CBM-20A" P. 4-20 Click to display the "Time Program" window. The time program can be edited Time-program from this window.
  • Page 66 Click to validate the settings and close the window. "Instrument" Tab Page  Click the [Instrument] tab to display the "Instrument" tab page. The monitor values for the components are displayed. The display contents vary depending on the components associated with the system. 2-26 CBM-20A/20Alite...

  • Page 67: Configuration" Tab Page

    Unit Configuration Used to select the method for assigning unit names. Used to select the pumping mode for the solvent delivery module Operation Mode and the operation mode for the autosampler and fraction collector. CBM-20A/20Alite 2-27...

  • Page 68: Editing" Tab Page

    Add-analysis button Used to assign the edited sequence to the analysis queue. The file selected in the "Analysis" tab page cannot be edited from this page. Refer to"2.7.1 "Analysis" Tab Page" P. 2-18 for details on other operations. 2-28 CBM-20A/20Alite...

  • Page 69: Queue" Tab Page

    Displays the scheduled end time for only the current line. Analyst Displays the name of the user that registered the analysis. Used to scroll the table up 24 lines. Used to scroll the table up 1 line. Used to scroll the table down 1 line. CBM-20A/20Alite 2-29...
  • Page 70 Used to insert WaitSeq lines. A WaitSeq line is inserted above the selected line. Used to delete sequence lines and WaitSeq lines. Two or more lines can be selected for deletion in one operation. 2-30 CBM-20A/20Alite...

  • Page 71: Error" Window

    If a fatal error occurs, in accordance with the message, click the Logout button button, close all windows, and reset the power to the system controller. It is also possible to clear all errors except fatal errors by pressing the initialization button on the back of the system controller. CBM-20A/20Alite 2-31...

  • Page 72: Precautions Related To Internet Explorer Operations

    When pop-up blocker software such as a security software or a toolbar for a browser is installed in the PC, Internet Explorer may not open the CBM-20A's Web application window. Please invalidate the pop-up block function for the URLs of the CBM or change the setting of the PC to disable the pop-up block function.

  • Page 73: Entering And Confirming Parameter Settings

    Note that, even if the paper size or scaling settings are changed, it may still not be possible to print out all the displayed contents on one sheet of paper. CBM-20A/20Alite 2-33...

  • Page 74: Continuous Operation

    Usually, limit use to one "Group Monitor" application and one "Analysis Execution" application.  Message during Operation When the network load grows with the CBM-20A Web application opened, the following message may appear. (1) Click the button to continue using the application. The Web application stops when the button is clicked, and the screen is not updated.

  • Page 75: Creating Shortcuts

    Files] in Internet Explorer when making this type of change in a system configuration. "Browser (Internet Explorer)" P. 6-4  Creating Shortcuts Right click on the desktop, select [New] and [Shortcut] to create a shortcut to the system controller's URL. CBM-20A/20Alite 2-35...
  • Page 76 2. Parts Identification and Function This page is intentionally left blank. 2-36 CBM-20A/20Alite...
  • Page 77 This chapter explains the settings that are required to allow several users to efficiently use several systems connected to the network. Contents Managing Several Systems as One Group ............. 3-2 Managing Several Users ................. 3-4 Starting the CBM-20A ..................3-6 Determine the System Configuration .............. 3-7...

  • Page 78: Managing Several Systems As One Group

    3. Preparation Managing Several Systems as One Group CBM-20A/20Alite controller can monitor several system controllers via the network. More specifically, the following operations are possible. • The status of analysis execution for several systems can be monitored. • System checks can be executed and the results can be viewed.
  • Page 79 3.1 Managing Several Systems as One Group In order to configure group settings, all the CBM-20A/20Alite System Controllers and SPD- M20A/M30A PDA Detectors must be in the same subnet, and the same group name must be set in the "Group Settings" application window. Consider factors such as the number of users for each system when deciding the group configuration.

  • Page 80: Managing Several Users

    In general, user information (user ID, user name, password, user level) is managed separately for each CBM-20A/20Alite System Controller. Up to 20 users can be registered per system. CBM-20A/20Alite, however, has a group settings function for copying (overwriting) all the user information for a system to other systems.

  • Page 81: Access Level

    The access levels are set for each system in a group and determine which users can log into each system. The Administrator is always authorized for access and all other users are authorized for access according to the default settings. Deny access if necessary. "5.9 Managing Groups" P. 5-39 CBM-20A/20Alite...

  • Page 82: Starting The Cbm-20A

    3. Preparation Starting the CBM-20A Use the following procedure to start the CBM-20A. Turn ON the power to the system controller and the components connected to it. Either turn ON the power to the system controller and the components simultaneously or turn ON the power to system controller last.

  • Page 83: Determine The System Configuration

    Click the appropriate icon or system name in the [System Name] column on the "Status" tab page in the "Status Summary" section. The "Login" window is displayed. Input the user ID in the [User ID] field. Input the password in the [Password] field. CBM-20A/20Alite...
  • Page 84 3. Preparation Click The "Analysis Execution" application window is displayed. Click the [Configuration] tab. The "Configuration" tab page is displayed. CBM-20A/20Alite...

  • Page 85: Checking Connections Between The System Controller And Components

    LC-20AB Binary Pump to Pump A Select this mode to use the binary gradient mode in combination with 2 B.GE isocratic pumps connected to Pump A and B. This enables the high- pressure gradient pumping of 2 solvents. CBM-20A/20Alite...
  • Page 86 The LC-20AB is connected as pumps A, B, C, and D in B.GEx2 mode. Even if a pump which is not compatible with a low-pressure gradient (such as the LC- 10AS) is connected in LP.GE mode, it will not operate properly. 3-10 CBM-20A/20Alite...

  • Page 87: Selecting The Autosampler Control Method

    Some functions will not operate properly if the configuration is changed with [Fixed] selected. The setting can be changed to [Auto] by initialization button. "9.2.6 Detector, Column Oven, and Solvent Delivery Module Connection and Setup" P. 9-16 CBM-20A/20Alite 3-11...

  • Page 88: Allocation With [Auto] Selected

    The default setting is [Auto]. Allocation when power to Pump.A and DET.A is turned OFF during control During control of all components PUMP.A DET.A PUMP.B DET.B PUMP.C Auto Fixed Auto Fixed PUMP.A DET.A PUMP.B DET.B PUMP.B PUMP.C Fig. 3.2 3-12 CBM-20A/20Alite...

  • Page 89: Making The Link Check Settings

    OFF the power to the system controller first. (If an SPD-M20A/M30A PDA Detector is used, however, turn OFF the power to the system controller or the detector and then turn OFF the power to the other within 10 seconds.) CBM-20A/20Alite 3-13...
  • Page 90 3. Preparation This page is intentionally left blank. 3-14 CBM-20A/20Alite...
  • Page 91 Basic Operation This chapter explains the basic operations required to perform analysis with the system controller. It provides a basic overall flow of operations handled by the system controller and describes how to create the method and sequence files required for analysis as well as time-program tables.

  • Page 92: Overall Flow Of Operation

    There is no fraction collector connected in this system configuration. P.4-3 Starting the System Components Starting Internet Explorer and Connecting to the P.4-3 CBM-20A Logging In and Displaying the "Analysis" Tab Page P.4-3 Selecting and Editing a Method File P.4-4 Selecting and Editing a Sequence File P.4-5...

  • Page 93: Starting The System Components

    Turn ON the power to the solvent delivery module, autosampler, column oven, detector, system controller, and PC. 4.1.2 Starting Internet Explorer and Connecting to the CBM-20A Start Windows Internet Explorer and connect to the system controller. "3.3 Starting the CBM-20A" P. 3-6 The "Status"...

  • Page 94: Selecting And Editing A Method File

    "Method" section. The "Load File" window is displayed. Select the method file used for analysis and click The window closes and the name of the selected file is displayed as the current method. "4.2 Creating Method Files" P. 4-10 CBM-20A/20Alite...

  • Page 95: Selecting And Editing A Sequence File

    The window closes and the name of the selected file is displayed as the current sequence. "4.3 Creating Sequence Files" P. 4-22 4.1.6 Preparing Samples Place the samples to be analyzed in the autosampler rack and close the door. CBM-20A/20Alite...

  • Page 96: Executing Autopurge

    Input the appropriate purge time (in minutes) for the solvent delivery module in the [Purge Time] column. The setting range is 1 to 20 minutes. (The setting unit is 1 minute and the default setting is 3 minutes.) CBM-20A/20Alite...
  • Page 97 During autopurge, the status display in the "Analysis" tab page changes in the following order: [A.Purge], [B.Purge], [SILPurge], [InitConcFlow], [WarmUp]. It returns to [Ready] when autopurge is completed. (The status display varies with the autopurge settings.) "2.7.1 "Analysis" Tab Page" P. 2-18 CBM-20A/20Alite...

  • Page 98: Waiting For The System To Stabilize

    If the status is [Stop], rinse the autosampler's needle by clicking 4.1.10 Turning OFF the Solvent Delivery Module and Column Oven When analysis is completed, click in the "Method" section to stop solvent delivery and temperature control. CBM-20A/20Alite...

  • Page 99: Quitting Analysis

    The "Group Monitor" application and Internet Explorer windows are closed. 4.1.12 Quitting the System Turn OFF the power to the components and quit windows. Turn OFF the power to the system controller, solvent delivery module, autosampler, column oven, and detector. Close Windows. CBM-20A/20Alite...

  • Page 100: Creating Method Files

    4.2.1 Selecting Method Files In order to create a new method file, it is first necessary to select a method file and load it. Click the [Analysis] tab in the "Analysis Execution" application window. The "Analysis" tab page is displayed. 4-10 CBM-20A/20Alite...

  • Page 101: Creating Method Files

    The window closes and the name of the selected file is displayed in the "Analysis" tab page as the current method. Click to close the window without loading a file. For details on , refer to "5.7 Managing Files" P. 5-25. CBM-20A/20Alite 4-11...

  • Page 102: Editing The "Method Parameter" Window

    The "Pump" tab pages in the "Method Parameter" window and the "Configuration Parameter" window are used here as examples. Click the tab for a different component to display the tab page for that component. "Pump" tab page in "Method Parameter" Window  4-12 CBM-20A/20Alite...

  • Page 103: Parameters For The Solvent Delivery Module

    "Configuration" tab page in the "Analysis Execution" application window. For details on the various parameters and the setting ranges, minimum units, units, and default values for the parameters, refer to "5.12.1 Pump" P. 5-57. CBM-20A/20Alite 4-13...
  • Page 104 If a pressure unit other than MPa is to be used, refer to "Selecting the Pressure Unit for the Pump" P. 4-15 before selecting the unit or setting the parameters. Set the method parameters for the pump. Click The "Pump" tab page in the "Configuration Parameter" window is displayed. 4-14 CBM-20A/20Alite...

  • Page 105: Selecting The Pressure Unit For The Pump

    The "Pump" tab page in the "Configuration Parameter" window is displayed. Select the required unit ([kgf/cm ], [psi], [MPa], or [bar]) from the [Pressure unit] pull-down menu. Click The "Configuration Parameter" window closes and the selected pressure unit is displayed in the [Maximum Pressure] and [Minimum Pressure] fields. CBM-20A/20Alite 4-15...

  • Page 106: Parameters For The Autosampler

    "5.12.2 Autosampler" P. 5-74. Click in the "Analysis" tab page. The "Autosampler" tab page in the "Method Parameter" window is displayed. Set the method parameters for the autosampler. Click The "Autosampler" tab page in the "Configuration Parameter" window is displayed. 4-16 CBM-20A/20Alite...

  • Page 107: Parameters For The Column Oven

    5-93. Click in the "Analysis" tab page. The "Oven" tab page in the "Method Parameter" window is displayed. Set the method parameters for the column oven. Click The "Oven" tab page in the "Configuration Parameter" window is displayed. CBM-20A/20Alite 4-17...
  • Page 108 The display returns to the "Method Parameter" window. Click The "Analysis" tab page is displayed. To set parameters for another component, click the tab for that component instead of clicking The corresponding tab page is displayed. 4-18 CBM-20A/20Alite...

  • Page 109: Parameters For The Detector

    "5.12.4 Detector" P. 5-96. Click in the "Analysis" tab page. The "Detector" tab page in the "Method Parameter" window is displayed. Set the method parameters for the detector. Click The "Detector" tab page in the "Configuration Parameter" window is displayed. CBM-20A/20Alite 4-19...

  • Page 110: Parameters For The Cbm-20A

    The corresponding tab page is displayed. 4.2.7 Parameters for the CBM-20A Enter the CBM-20A parameters, such as the event output and serial interface. For details on the various parameters and the setting ranges, minimum units, units, and default values for the parameters, refer to "5.13.6 System Controller"...
  • Page 111 The display returns to the "Method Parameter" window. Click The "Analysis" tab page is displayed. To set parameters for another component, click the tab for that component instead of clicking . The corresponding tab page is displayed. CBM-20A/20Alite 4-21...

  • Page 112: Creating Sequence Files

    4.3.1 Selecting Sequence Files In order to create a new sequence file, it is first necessary to select a sequence file and load Click the [Analysis] tab in the "Analysis Execution" application window. The "Analysis" tab page is displayed. 4-22 CBM-20A/20Alite...

  • Page 113: Creating Sequence Files

    The window closes and the name of the selected file is displayed in the "Analysis" tab page as the current sequence. Click to close the window without loading a file. For details on , refer to "5.7 Managing Files" P. 5-25. CBM-20A/20Alite 4-23...
  • Page 114 For details on rack and sample numbers, refer to "5.14 List of Rack Numbers" P. 5- 147. Input the number of injections for each vial. The Injections/Vial setting range is 1 to 99 and the default setting is 4-24 CBM-20A/20Alite...
  • Page 115 Click to delete the input data for all lines. All-clear button Used to scroll the table up 10 lines. Used to scroll the table up 1 line. Used to scroll the table down 10 lines. Used to scroll the table down 1 line. CBM-20A/20Alite 4-25...

  • Page 116: Creating Sequence Tables

    Up to 100 lines can be input in a sequence table. The sequence is composed in order starting from line 1. It is not possible to skip lines. When the required settings have been made in the sequence table, the table is saved as a sequence file. 4-26 CBM-20A/20Alite...

  • Page 117: Editing Sequence Tables

    In the sequence table, click the cell in front of the first line to be cut or copied. The selected line turns light blue. Click the button. When inserting or pasting, this inserts the lines that were copied by cutting or copying. When inserting one line, the contents of the selected line are inserted. CBM-20A/20Alite 4-27...

  • Page 118: Gradient Analysis Using Time Programs

    The "Analysis" tab page is displayed. Select the method file for which the time program is to be created. The selection method is the same as the one described in "Selecting Method Files". "4.2 Creating Method Files" P. 4-10 4-28 CBM-20A/20Alite...
  • Page 119 4.4.2 Table and Buttons Used for Time Programs The component function parameters are entered in the time-program table and analysis is performed according to these set times. This section explains the time-program table and the functions of the buttons. CBM-20A/20Alite 4-29...
  • Page 120 ("ISO", "B.GE", "T.GE", "LP.GE"), [Sort by Time] and [Sort by detector ("Detector"), CBM-20A ("Controller"), autosampler Module] buttons ("Autosampler"), column oven ("Oven"), sub-controller ("Subc") and Fraction Collector (FRC).

  • Page 121: Creating Time-Program Tables

    Input the run time for the first line in the [Time] column and press the [Tab] key. It becomes possible to make selections from the pull-down menus in the [Module] and [Function] columns. If input is required in the [Value] column, the default value for that parameter is displayed. CBM-20A/20Alite 4-31...
  • Page 122 The time program is saved to the selected method file and the "Time Program" window closes. To perform analysis using time programs, select the appropriate method files in the sequence table's [Method] column. For details on analysis procedures, refer to "4.1 Overall Flow of Operation" P. 4-2. 4-32 CBM-20A/20Alite...

  • Page 123: Time-Program Setting Example

    Time Module Function Value B. GE1 B. CONC 80.0 B. GE1 B. CONC 80.0 15.01 B. GE1 B. CONC Controller STOP Be sure to set the STOP command for the "Controller" at the end of the time program. CBM-20A/20Alite 4-33...

  • Page 124: Starting Analysis

    Canceling "Cooling Down" or "Power Down" If a shutdown is set in the analysis queue, [Cooling Down] or [Power Down] is displayed in the status display after the sequence has been completed. If is clicked, the shutdown is canceled and [Ready] is displayed. 4-34 CBM-20A/20Alite...

  • Page 125: Changing Parameters During Analysis

    Analysis can also be started via input to the MAN.INJ. connector on the back of the system controller. If a sequence table is set, after the analysis for one step in the table is completed, proceed to the next step by clicking CBM-20A/20Alite 4-35...

  • Page 126: Printing Files

    Select "Printer" and the item to print out. Fraction Report cannot be selected. Click the [OK] button. The "Method Report" or "Sequence Report" window will be displayed. Select [Files] - [Print] in the menu of Internet Explorer. 4-36 CBM-20A/20Alite...

  • Page 127: Print To Chromatopac

    Fraction Report can be select only when fraction results exist. To print out a fraction result report after each analysis, set "Enable printing on fraction result" to ON in the system controller's configuration parameters. Click the [OK] button. The selected item will be printed out to Chromatopac. CBM-20A/20Alite 4-37...
  • Page 128 4. Basic Operation This page is intentionally left blank. 4-38 CBM-20A/20Alite...
  • Page 129 Application Operation This chapter provides descriptions on the system controller's various analysis methods, such as sequential analysis using queues and analysis using a fraction collector, as well as the file and group settings used for analysis. Contents Injecting Standard Samples at Regular Intervals ..........5-2 Setting the Mobile Phase Reserve Volume .............

  • Page 130: Chapter 5 Application Operation

    (3) Lines 1 to 3 of the standard-sample injection table are executed again. (4) Lines 5 to 8 of the sequence table are executed. (5) Lines 1 to 3 of the standard-sample injection table are executed again. (6) Line 9 of the sequence table is executed and analysis is completed. CBM-20A/20Alite...

  • Page 131: Creating Standard-Sample Injection Tables

    The method for creating a table from the "Analysis" tab page is described below. Click the [Analysis] tab in the "Analysis Execution" application window. The "Analysis" tab page is displayed. Select a sequence file. "4.3.1 Selecting Sequence Files" P. 4-22 CBM-20A/20Alite...
  • Page 132 [Every n lines] field in the [Standard Sample] tab. Click the [Sequence] tab. Standard-sample insertion marks are displayed between the lines of the sequence at the appropriate positions and the standard-sample insertion table is saved together as part of the sequence file. CBM-20A/20Alite...

  • Page 133: Setting The Mobile Phase Reserve Volume

    5.2.1 Displaying the "Mobile Phase Reserve Volume Setting" Window The procedure for displaying the "Mobile Phase Reserve Volume Setting" window is explained below. Click the [Analysis] tab in the "Analysis Execution" application window. The "Analysis" tab page is displayed. CBM-20A/20Alite...
  • Page 134 Click to reset the [Default Volume] setting. If (additional) mobile phase has been set in the reservoir, set the mobile-phase volume and click this button. The data is sent to the solvent delivery module. (Same applies to the rinse liquid.) CBM-20A/20Alite...

  • Page 135: Checking The Mobile Phase Reserve Volume

    Click to close this window. 5.2.2 Checking the Mobile Phase Reserve Volume Use the procedure below to check the mobile phase reserve volume. Click the [Analysis] tab. The "Analysis" tab page is displayed. CBM-20A/20Alite...
  • Page 136 The reserve volume for the selected solvent delivery module is displayed. The reserve volume for the autosampler rinse solution is always displayed. If the reserve volume drops below the set threshold, the following window is displayed. Check the mobile phase reserve volume. CBM-20A/20Alite...

  • Page 137: Executing Autopurge For Solvent Delivery Modules And Autosamplers

    • Autopurge can be executed while the autosampler is not running. • The purge execution time cannot be set if an SIL-10A-series Autosampler is connected. 1. If the connected autosampler is the SIL-30AC, autopurge cannot be executed when the loop injection is set for the injection type. CBM-20A/20Alite...

  • Page 138: Analysis Using Queues

    Perform shutdown settings. P.5-14 Perform startup settings. P.5-14 Start analysis. P.5-13 Stop analysis. Shutdown is executed P.5-14 (if shutdown execution is set). Startup is executed P.5-14 (if shutdown execution is set). Preparation for the next analysis is completed. 5-10 CBM-20A/20Alite...

  • Page 139: Registering Sequence Files To The Analysis-Queue Tables

    "4.3.1 Selecting Sequence Files" P. 4-22 Click in the "Sequence" section. The sequence file is registered to the analysis-queue table. Repeat steps 2 and 3 until all the required sequence files are registered to the analysis- queue table. CBM-20A/20Alite 5-11...

  • Page 140: Editing Analysis-Queue Tables

    Select the sequence file to be deleted from the analysis-queue table by clicking the box on the left of the filename. The selected line turns light blue. Click The message [Selected line will be deleted. Continue?] is displayed. Click The sequence file is deleted. 5-12 CBM-20A/20Alite...

  • Page 141: Putting The Analysis Queue In Waiting Status

    Waiting status is cleared and the next analysis sequence is executed.  Clearing Waiting Status from the "Queue" Tab Page Click the [Queue] tab. The analysis-queue table is displayed. Click Waiting status is cleared and the next analysis sequence is executed. CBM-20A/20Alite 5-13...

  • Page 142: Shutdown And Startup Settings

    Shutdown and startup settings are performed in the "Queue" tab page. Click the [Queue] tab. The "Queue" tab page is displayed. For details on the analysis-queue table on the left of the page, refer to "5.4 Analysis Using Queues" P. 5-10. 5-14 CBM-20A/20Alite...

  • Page 143: Shutdown And Startup Parameters And Buttons

    "Execute Shutdown" check box is selected. The pull-down menu contains 12 files, StartUp00 to StartUp11. Set the details for the startup methods in these files. Program File The names of these files cannot be changed. Click to display the "Advanced Startup Settings" window. CBM-20A/20Alite 5-15...

  • Page 144: Advanced Shutdown Settings" Window

    Select the file (ShutDown00 to ShutDown11) in which to save the advanced settings from the [Program File] pull-down menu. Input the column's cooldown time. The setting range is 0.01 to 120.00 (minutes), the setting unit is 0.01, and the default setting is 10.00 (minutes). 5-16 CBM-20A/20Alite...
  • Page 145 OFF when shutdown is executed. Click The settings are saved and the window closes. The cooldown-time setting and the settings made under "Step 2: System Shutdown" are all reflected in the "Startup/Shutdown Setting" section. CBM-20A/20Alite 5-17...

  • Page 146: Advanced Startup Settings" Window

    The "Advanced Startup Settings" windows is displayed. The contents displayed in the window vary with the type of solvent delivery module and autosampler connected. Select the file (StartUp00 to StartUp11) in which to save the advanced settings from the [Program File] pull-down menu. 5-18 CBM-20A/20Alite...
  • Page 147 [Set flow rate at half of that in method.] check box. If the [Set flow rate at half of that in method.] check box is selected, input the pumping time in the [Warmup Time] field. The input range is 1 to 120 (minutes). CBM-20A/20Alite 5-19...
  • Page 148 Click The settings are saved and the window is closed. The settings for month, day, hour, and minute of startup execution as well as the items of the method file are all reflected in the "Startup/Shutdown Setting" section. 5-20 CBM-20A/20Alite...

  • Page 149: Analysis Using Fraction Collectors

    A time program controls the fraction collector in the same way as other components. If a rack other than a Shimadzu rack is used, teaching of the custom rack must be performed for the fraction collector.

  • Page 150: Controlling The Fraction Collector With A Time Program

    5.6.4 Setting the Number and Positions of Vials in Racks If using a fraction collector rack other than a Shimadzu rack, the number and positions of vials must be stored in the system. Enter the rack information in the "Fraction Collector Rack Adjustment"...

  • Page 151: Functions Of Buttons

    Used to change the scale for position adjustment. The scale switches between [×1] (2 mm) and [×10] (20 mm) each time the button is clicked. Used for position adjustment of the fraction collector's arm. The arm operates according to the scale set with the scale-change button. CBM-20A/20Alite 5-23...

  • Page 152: Adjusting The Rack Position

    Move the fraction collector's arm to the position of the right-back vial using the position- adjustment buttons and click The right-back position is set and the window closes. Click to cancel the settings. The window closes without saving the settings. 5-24 CBM-20A/20Alite...

  • Page 153: Managing Files

    Files can be saved to the PC from either the "Analysis" tab page or the "Editing" tab page. The procedure for saving files from the "Analysis" tab page is described below. Click the [Analysis] tab. The "Analysis" tab page is displayed. CBM-20A/20Alite 5-25...
  • Page 154 . Clicking this button and associating the file with another application may make it impossible to save the file. If this button is inadvertently clicked and the "Open With" window is displayed, close the window by clicking [Cancel]. 5-26 CBM-20A/20Alite...
  • Page 155 The file is saved in the selected location and the following window is displayed. Click in the "Download complete" window. The window closes. Repeat steps 3 to 9 until all the required files are saved. To end the saving procedure, click in the "Save file" window. The "Save file" window closes. CBM-20A/20Alite 5-27...

  • Page 156: Creating New Files Using Existing Files

    "Method" section to save method files and click in the "Sequence" section to save sequence files. The "Save file" window is displayed. Select the file to be copied from the list on the left of the window. 5-28 CBM-20A/20Alite...

  • Page 157: Downloading Files From A Pc

    Files saved to a PC can be downloaded from either the "Analysis" tab page or the "Editing" tab page. The procedure for downloading files from the "Analysis" tab page is described below. CBM-20A/20Alite 5-29...
  • Page 158 5. Application Operation Click the [Analysis] tab. The "Analysis" tab page is displayed. Click in the "Method" section to download method files and click in the "Sequence" section to download sequence files. The "Load File" window is displayed. 5-30 CBM-20A/20Alite...
  • Page 159 The filename can be changed by inputting a new filename in the [New file name] field. Up to 8 characters can be entered for method files and up to 10 characters for sequence files. The symbols "-" (hyphen) and "_" (underscore) can be used. CBM-20A/20Alite 5-31...
  • Page 160 The following window is displayed. Click This completes the procedure for downloading a file. Repeat steps 3 to 9 until all the required files are downloaded. To end the downloading procedure, click in the "Load File" window. The "Load File" window closes. 5-32 CBM-20A/20Alite...

  • Page 161: Managing Component Parameters

    5.8.1 Saving All Component Parameters to a PC All the parameters in the method and sequence files can be saved in one operation. This operation is performed from the "Utility" window. Click in the "Analysis Execution" application window. The "Utility" window is displayed. CBM-20A/20Alite 5-33...
  • Page 162 If this button is inadvertently clicked and the "Open With" window is displayed, close the window by clicking [Cancel]. Click The following window is displayed. Select the save location. Input the filename. 5-34 CBM-20A/20Alite...

  • Page 163: Downloading All Component Parameters From A Pc

    All the parameters saved from method files and sequence files to a PC can be downloaded together. This operation is performed from the "Utility" window. This operation can be performed only by Administrators or Power Users. Click in the "Analysis Execution" application window. The "Utility" window is displayed. CBM-20A/20Alite 5-35...
  • Page 164 5. Application Operation Click or [Load All Parameters]. The following window is displayed. Input the user password. Click The [Load All Parameters] window is displayed. Click next to the [File Name] field. The following window is displayed. 5-36 CBM-20A/20Alite...

  • Page 165: Initializing Parameters

    This operation is performed from the "Utility" window. WARNING Performing this operation returns all parameters in method and sequence files, including configuration parameters, to their default settings. This operation can be performed only by Administrators or Power Users. CBM-20A/20Alite 5-37...
  • Page 166 [Initialize All Parameters]. The following window is displayed. Input the user password. Click The following window is displayed. Close the browser and turn OFF the power to the system controller. The parameters are initialized when power is turned ON. 5-38 CBM-20A/20Alite...

  • Page 167: Managing Groups

    "9.3.2 Making TCP/IP-related Settings" P. 9-30 5.9.1 Displaying the "Group Settings" Application Window Use the following procedure to display the "Group Settings" application window. Click or the system name in the "Status" tab page. The "Login" window is displayed. CBM-20A/20Alite 5-39...
  • Page 168 Logout button. This button is displayed with all 3 tab pages. If it is clicked, the "Group Settings" application window is closed and the "Group Monitor" application window is displayed. 5-40 CBM-20A/20Alite...

  • Page 169: Group Information" Tab Page

    User Information Table Displays up to 20 items of user information. User ID Displays the registered user IDs. User Name Displays the names of the registered users. User Level Displays the access level set for the user. CBM-20A/20Alite 5-41...

  • Page 170: Sharing And Centralizing User Information For Systems In A Group

    Changes in user information are not reflected in systems with power turned OFF. Click [Copy the User Information]. Set the access users for each system in the "System Administration" tab page. "5.9.3 "System Administration" Tab Page" P. 5-43 5-42 CBM-20A/20Alite...

  • Page 171: System Administration" Tab Page

    Used to set the subnet mask of the network to which the system Subnet Mask controller is connected. Master System [None] is always displayed. Input information such as the purpose of the server. Up to 30 About this System characters can be entered. CBM-20A/20Alite 5-43...

  • Page 172: Advanced Settings" Window

    Ethernet Mode Select the Ethernet mode from the pull-down menu. Specify the location of the Help file by URL. Specify location of Under normal circumstances, do not change the default setting. external Help file. (The default setting is "/help".) 5-44 CBM-20A/20Alite...

  • Page 173: Changing System Names

    Log into the "Group Settings" application again. "5.9.1 Displaying the "Group Settings" Application Window" P. 5-39 Click the [System Administration] tab. The "System Administration" tab page is displayed. Click The following window is displayed. CBM-20A/20Alite 5-45...

  • Page 174: Moving Systems To Different Groups

    Select the system to move from the [System to Edit] pull-down menu. Select the group in which the system is to be moved to from the [Group] pull-down menu. Click The window closes and the user is automatically logged out. The "Group Monitor" application window is displayed. 5-46 CBM-20A/20Alite...

  • Page 175: Creating New Groups

    Up to 15 characters, consisting of alphanumeric characters and the symbols "-" (hyphen) and "_" (underscore), can be entered. Click Click The window closes and the user is automatically logged out. The "Group Monitor" application window is displayed. CBM-20A/20Alite 5-47...

  • Page 176: User Registration

    The "User Management" tab page is used to register new users and to edit user information for currently registered users. This page is displayed by clicking the [User Management] tab in the "Group Settings" application. For details on sharing user information within a group, refer to "5.9.2 "Group Information" Tab Page" P. 5-41. 5-48 CBM-20A/20Alite...

  • Page 177: Registering New Users

    "Group Settings" application. The "Login" window is accessed from the "Status" tab page of the "Analysis Execution" application. Enter a user ID and password, and click [Group Settings]. The "Group Settings" application window is displayed. CBM-20A/20Alite 5-49...
  • Page 178 Authorized to perform all the operations related to analysis (e.g., editing methods and sequences, executing, adding, and stopping PowerUser analyses, and executing system checks) and to change system configurations. Also authorized to clear errors or forcibly stop analysis being performed by other users. 5-50 CBM-20A/20Alite...
  • Page 179 If the password is not input correctly, an "Error" window is displayed. In this case, close the window and input an acceptable password. Repeat steps 1 to 8 as necessary to register more users. Click The "Logout" window is displayed. Click The "Logout" window closes and the "Status" tab page is displayed. CBM-20A/20Alite 5-51...

  • Page 180: Changing User Information

    Users currently logged into the "Group Settings" application cannot be deleted. Select the user ID to be deleted from the [User ID] pull-down menu. becomes active. Click The selected user ID is deleted from the [User ID] field. 5-52 CBM-20A/20Alite...

  • Page 181: User Levels And Functions

      errors System check Performing system checks     : Available  : Only the solvent delivery module’s flow rate and autosampler cooler temperature can be set. Empty : Not available CBM-20A/20Alite 5-53...
  • Page 182 Logged out During Web analysis   Other than the above    During connection from LC-WS A user can only be forcibly logged out from a PC other than that on which they are logged in. 5-54 CBM-20A/20Alite...

  • Page 183: Changing Passwords

    Administrator to copy the new password setting to other systems using the [Copy the User Information] function from the "Group Information" tab page. "5.9.2 "Group Information" Tab Page" P. 5-41 CBM-20A/20Alite 5-55...

  • Page 184: Locking Systems

    The following window is displayed. Input the password. Click and close the window. changes to . (In the case of system lock.) The following window is displayed when attempting to log into a system which has been locked. 5-56 CBM-20A/20Alite...

  • Page 185: Parameter Details

    Parameter Details The basic parameters and configuration parameters for the system controller and system components are explained below. For details on parameter entry procedures, refer to "4.2.7 Parameters for the CBM-20A" P. 4-20. 5.12.1 Pump  Comparison of Parameters for Different Models...
  • Page 186 10 to 1326 142 to 19000 1451 10 to 1300 Minimum Pressure: 0.0 to 130.0 kgf/cm 0 to 1326 0 to 19000 0 to 1300 Solenoid Valve Sets the position of the 4-position 1, 2, 3, 4 (FCV-10AL type) valve. 5-58 CBM-20A/20Alite...
  • Page 187 0 to 400 Solenoid Valve Sets the position of the 4-position 1, 2, 3, 4 (FCV-10AL type) valve. 1a, 1b/ Solenoid Valve 2a, 2b/ 1a/2a/3a Sets position a or b for flow lines 1 to 3. (FCV-11AL type) 3a, 3b CBM-20A/20Alite 5-59...
  • Page 188 (FCV-10AL type) valve. 1a, 1b/ Solenoid Valve 2a, 2b/ 1a/2a/3a Sets position a or b for flow lines 1 to 3. (FCV-11AL type) 3a, 3b 1. The LP.GE mode is not available on the LC-20ADXR earlier than version 1.30. 5-60 CBM-20A/20Alite...
  • Page 189 4-cycle Solenoid Valve Sets the position of the 4-position 1, 2, 3, 4 (FCV-10AL type) valve. 1a, 1b/ Solenoid Valve 2a, 2b/ 1a/2a/3a Sets position a or b for flow lines 1 to 3. (FCV-11AL type) 3a, 3b CBM-20A/20Alite 5-61...
  • Page 190 0 to 300 Solenoid Valve Sets the position of the 4-position 1, 2, 3, 4 (FCV-10AL type) valve. 1a, 1b/ Solenoid Valve 2a, 2b/ 1a/2a/3a Sets position a or b for flow lines 1 to 3. (FCV-11AL type) 3a, 3b 5-62 CBM-20A/20Alite...
  • Page 191 0 to 392 Solenoid Valve Sets the position of the 4-position 1, 2, 3, 4 (FCV-10AL type) valve. 1a, 1b/ Solenoid Valve 2a, 2b/ 1a/2a/3a Sets position a or b for flow lines 1 to 3. (FCV-11AL type) 3a, 3b CBM-20A/20Alite 5-63...
  • Page 192 4-cycle Solenoid Valve Sets the position of the 4-position 1, 2, 3, 4 (FCV-10AL type) valve. 1a, 1b/ Solenoid Valve 2a, 2b/ 1a/2a/3a Sets position a or b for flow lines 1 to 3. (FCV-11AL type) 3a, 3b 5-64 CBM-20A/20Alite...
  • Page 193 4-cycle Solenoid Valve Sets the position of the 4-position 1, 2, 3, 4 (FCV-10AL type) valve. 1a, 1b/ Solenoid Valve 2a, 2b/ 1a/2a/3a Sets position a or b for flow lines 1 to 3. (FCV-11AL type) 3a, 3b CBM-20A/20Alite 5-65...
  • Page 194 Concentration A cannot be edited directly. Concentration A = 100 - Concentration B - Concentration C (%) Setting possible when LP.GE, B.GE, Concentration B (%) 0.0 to 100.0 T.GE or B.GEx2 is set as the pumping mode. (See note 1.) 5-66 CBM-20A/20Alite...
  • Page 195 B.GEx2 is set as the pumping mode. Setting possible when T.GE is set as Concentration C (%) 0.0 to 100.0 the pumping mode. Maximum Pressure: 1.0 to 50.0 kgf/cm 10 to 510 142 to 7260 1422 10 to 500 CBM-20A/20Alite 5-67...
  • Page 196 10 to 440 142 to 6260 1422 10 to 432 Minimum Pressure: 0.0 to 39.2 0 to 400 kgf/cm 0 to 5690 0 to 392 Solenoid Valve Sets the position of the 3-position (FCV-130AL/FCV-130BL 0, 1, 2 valve. type) 5-68 CBM-20A/20Alite...
  • Page 197 10 to 510 142 to 7260 1422 10 to 500 Minimum Pressure: 0.0 to 49.0 kgf/cm 0 to 500 0 to 7110 0 to 490 Solenoid Valve Sets the position of the 2-position 0, 1 (FCV-7AL/ type) valve. CBM-20A/20Alite 5-69...
  • Page 198 PMAX RANGE = 0 PMAX RANGE = 1 PMAX RANGE = 1 21.6 MPa 29.4 MPa 21.6 MPa LC-10Ai (220 kgf/cm (300 kgf/cm (220 kgf/cm 22 MPa 30 MPa 22 MPa LC-20Ai (224 kgf/cm (306 kgf/cm (224 kgf/cm 5-70 CBM-20A/20Alite...
  • Page 199 50 mL< Flow Rate Flow Rate  50 mL 100 mL< Flow Rate  100 mL 44.0 MPa 33.0 MPa LC-20AP (449 kgf/cm (337 kgf/cm 31.4 MPa 19.6 MPa 9.8 MPa LC-8A (320 kgf/cm (200 kgf/cm (100 kgf/cm CBM-20A/20Alite 5-71...
  • Page 200 3. This setting can be made for each solvent delivery module. Set the valve type at each solvent delivery module beforehand. 4. Select [Used] if a solenoid valve is connected. 5. Available only with LC-30AD. 6. For LC-30ADs earlier than version 2.20, the solvent blending cannot be used. 5-72 CBM-20A/20Alite...
  • Page 201 1. Pumping stops when the error is cleared. 2. This setting can be made for each solvent delivery module. Set the valve type at each solvent delivery module beforehand. 3. Select [Used] if a solenoid valve is connected. CBM-20A/20Alite 5-73...

  • Page 202: Autosampler

    Stack D Needle Stroke    Stack C Needle Stroke    Stack B Needle Stroke    Stack A Sample Discharge  Speed Measuring Line   Purge Volume Air Gap Volume   5-74 CBM-20A/20Alite...
  • Page 203      With external input Overlap Injection        Rinse Pump Setting     Injection Type   Maximum Injection     Volume Loop Volume  CBM-20A/20Alite 5-75...
  • Page 204 [Equilibration Start Time] and [Equilibration Hold Time] settings are displayed when direct injection is set for the injection type. The [Sample Discharge Speed], [Loop Injection Type], [Excess Volume] and [Loop Fill Factor] settings are displayed when loop injection is set for the injection type. 5-76 CBM-20A/20Alite...
  • Page 205 OFF (Fast LC): No needle rinsing while fixing some parameters to shorten the analysis cycle. (See note 3.) Rinse setting Set the purge volume (low-pressure Rinse Volume (L) 0 to 2000 flow line purge volume) for the rinse port after injection. (See note 2.) CBM-20A/20Alite 5-77...
  • Page 206 0.0 to 25.0 10.0 with rinse solution R2. (See note 2.) R2 (min) Internal Rinse Settings After Analysis Set the timing to start the internal Rinsing Start Timing Specify Start After Analysis rinsing of the needle. (See note 2.) Time 5-78 CBM-20A/20Alite...
  • Page 207 3. For details about the parameters that are fixed when "OFF (Fast LC)" is selected for [Rinse Type], refer to the SIL- 30ACMP instruction manual. 4. For details about sample loop equilibration, refer to the SIL-30ACMP instruction manual. CBM-20A/20Alite 5-79...

  • Page 208: Autosampler

    Air Gap Volume (L) 0 to 5.0 Injection: 0 and after the aspirated sample. Loop Injection: 0.1 Two loop injection methods are Partial Loop Loop Injection Type Partial Loop available. Select either partial loop Full Loop method or full loop method. 5-80 CBM-20A/20Alite...
  • Page 209 (See note Set the time for which the needle is dipped in rinse solution at the rinse Rinse Dip Time (sec) 0 to 60 port. Enabled for [Rinse Mode] settings other than "No rinse". (See note 2.) CBM-20A/20Alite 5-81...
  • Page 210 Check the box when performing R0: Off rinsing of the injection port after Injection Port Rinsing R0/ On, Off R1: Off internal rinsing of the needle. Only the R1/R2 R2: Off rinse solutions specified at [Rinsing Sequence] are enabled. (See note 2.) 5-82 CBM-20A/20Alite...
  • Page 211 2. Depending on the rinse type setting, this setting is shown or hidden and enabled or disabled. 3. For details about the parameters that are fixed when "OFF (Fast LC)" is selected for [Rinse Type], refer to the SIL-30AC instruction manual. 4. For details about sample loop equilibration, refer to the SIL-30AC instruction manual. CBM-20A/20Alite 5-83...
  • Page 212 Sets the solution-replacement speed for the rinse port after injection. If the rinse solution has a high viscosity, Rinse Speed (µL/sec) 1 to 35 bubbles may form in the low-pressure flow line and so set a speed appropriate to the viscosity. 5-84 CBM-20A/20Alite...
  • Page 213 Cooling Rack 14, 15 Needle Stroke (mm) Standard Rack 2 20 to 43 (See note 1.) Cooling Rack 12 Sets the time for which a low-pressure Purge Time (min) 0.1 to 25.0 25.0 flow line is purged with the measuring pump. CBM-20A/20Alite 5-85...
  • Page 214 1, 2 (See note 1.) 1. If Needle Stroke and MTP Tray are changed in one method file, these changes are applied to all other files (No. 0 to 19), because they are depend on the sample rack. 5-86 CBM-20A/20Alite...
  • Page 215 Sets the speed at which the measuring Sampling Speed (µL/sec) 10 to 150 pump measures samples. Sets the solution-replacement volume (low-pressure flow line's solution- Rinse Volume (µL) 1 to 20000 5000 replacement volume) for the rinse port after injection. CBM-20A/20Alite 5-87...
  • Page 216 If this setting is enabled, when executing multiple analysis, injection for the next analysis is started before Disabled Overlap Injection Disabled completion of the current analysis, and Enabled the next analysis is started immediately after the current analysis is completed. (See note 2.) 5-88 CBM-20A/20Alite...
  • Page 217 Disabled: The autosampler injects the sample immediately after loading it. Disabled Synchronize injection with Disabled Enabled: After loading the sample, the autosampler external input. (INJIN) Enabled waits for a contact input signal (MAN.INJ.) before injecting the sample. (See note 1.) CBM-20A/20Alite 5-89...
  • Page 218 If this setting is enabled, when executing multiple analyses, injection for the next analysis is started Disabled Overlap Injection Disabled before completion of the current analysis, and the Enabled next analysis is started immediately after the current analysis is completed. (See note 2.) 5-90 CBM-20A/20Alite...
  • Page 219 1. If there is a contact input signal before the sample is loaded, the sample is injected as soon as loading is completed. 2. The time at which injection is started is calculated automatically according to the analysis completion time and the type of autosampler connected. CBM-20A/20Alite 5-91...
  • Page 220 2. The time at which injection is started is calculated automatically according to the analysis completion time and the type of autosampler connected. 3. 5000µL syringe is available only with SIL-10A version 4.10 or later and SIL-10AF/10AP. Do not select 5000µL syringe, SIL-10A earlier than version 4.10, or SIL-10Ai/10AXL. 5-92 CBM-20A/20Alite...
  • Page 221 Total Injection Vol.    Usage Time Limit The [Valve Position] setting is displayed when [Used] is selected in the [Solenoid Valve] field in the "Configuration Parameter" window. [CMD] is displayed when a column module device is connected. CBM-20A/20Alite 5-93...
  • Page 222 4, 5, 6 R and L. 6 Position Valve L  CTO-10Avp, CTO-10ACvp, CTO-10ASvp, CTO-10A, CTO-10AC Parameter Setting range Minimum unit Default setting Remark Oven Temperature (ºC) 0, 4 to 80 0 means OFF. Maximum Temperature (ºC) 5 to 85 5-94 CBM-20A/20Alite...
  • Page 223 4294967295 0 to Total Injection No. Setting not possible. 4294967295 0 to Total Injection Vol. (µL) Setting not possible. 4294967295 Usage Time Limit 10 bytes max. (See note 1.) 1. A Unicode character string (UTF-8) can be entered. CBM-20A/20Alite 5-95...

  • Page 224: Detector

     AUX Range1   AUX Range2   AUX Range3   AUX Range4   Bandwidth1   Bandwidth2   Bandwidth3   Bandwidth4   Recorder Range    Recorder Range2   5-96 CBM-20A/20Alite...
  • Page 225 Slit Width   Reference   Compensation Reference   Wavelength Reference   Bandwidth [SV Level] and [SV Delay Time] are displayed when [Used] is selected for the solvent recycle valve on the "Configuration Parameter" window. CBM-20A/20Alite 5-97...
  • Page 226 Default setting Remark D2 lamp/single Wavelength1 (nm) 190 to 370 D2 lamp/dual Wavelength1 (nm) 190 to 370 Displayed if [Dual] is selected as the Wavelength2 (nm) 190 to 370 wavelength mode. W lamp/single Wavelength1 (nm) 371 to 900 5-98 CBM-20A/20Alite...
  • Page 227 9 to 50  SPD-10Avp Parameter Setting range Minimum unit Default setting Remark Single Wavelength1 (nm) 190 to 600 Dual 190 to 370 Use the same setting range for both Wavelength1 (nm) 371 to 600 wavelength 1 and wavelength 2. CBM-20A/20Alite 5-99...
  • Page 228 1 and wavelength 2. 371 to 700 Displayed if [Dual] is selected as the Wavelength2 (nm) 701 to 900 wavelength mode. Common parameters Single Wavelength Mode Single Dual Lamp Off, D2, W 0.5, 1, 2, AUX Range (AU/V) 4, 1.25, 2.5 5-100 CBM-20A/20Alite...
  • Page 229 Default setting Remark D2 lamp/single Wavelength1 (nm) 190 to 370 D2 lamp/dual Wavelength1 (nm) 190 to 370 Displayed if [Dual] is selected as the Wavelength2 (nm) 190 to 370 wavelength mode. W lamp/single Wavelength1 (nm) 371 to 900 CBM-20A/20Alite 5-101...
  • Page 230 200 to 900 (nm) wavelength mode. Single Wavelength Mode Single Dual Lamp On, Off Recorder Range2 0 to 9 No Filter, 0.05, 0.1, 0.5, Response (sec) 1.0, 1.5, 3.0, 6.0, 8.0, 10.0, Gain x1, x4, x16 High Sensitivity Middle Middle 5-102 CBM-20A/20Alite...
  • Page 231 Minimum unit Default setting Remark Excitation Wavelength (nm) 200 to 900 Emission Wavelength (nm) 200 to 900 Lamp On, Off Recorder Range 0 to 9 0.1, 0.5, Response (sec) 1.5, 3.0 Gain x1, x4, x16 High Sensitivity Middle Middle CBM-20A/20Alite 5-103...
  • Page 232 8.0, 10.0, 2.0 Polarity 1.0E-04 1.0E-03 AUX Range (RIU/V) 1.0E-03 1.0E-02 2.5E-04 Temperature Control On, Off Cell Temperature (ºC) 30.0 to 60.0 40.0 0.00 to SV Level (μRIU) 0.01 9999.00 9999.00 SV Delay Time (sec) 0.0 to 99.9 5-104 CBM-20A/20Alite...
  • Page 233 190 to 700 Wavelength3 (nm) Wavelength4 (nm) Lamp Off, D2 AUX Range1 (AU/V) AUX Range2 (AU/V) 0.5, 1, 2, AUX Range3 (AU/V) 4, 1.25, 2.5 AUX Range4 (AU/V) Bandwidth1 (nm) Bandwidth2 (nm) 0 to 500 Bandwidth3 (nm) Bandwidth4 (nm) CBM-20A/20Alite 5-105...
  • Page 234 4, 1.25, 2.5 AUX Range4 (AU/V) Bandwidth1 (nm) Bandwidth2 (nm) 1 to 50 Bandwidth3 (nm) Bandwidth4 (nm) None, 25, 80, 160, 240, 320, Response (msec) 2000 640, 1280, 2000 Polarity Temperature Control On, Off Cell Temperature (ºC) 9 to 50 5-106 CBM-20A/20Alite...
  • Page 235 Minimum unit Default setting Remark 5, 10, 25, 80, Sampling Rate (msec) 160, 240, 320, 640, 1280, 2000 Slit Width 1, 8 Reference Compensation On, Off Reference Wavelength 190 to 700 (nm) Reference Bandwidth (nm) 0 to 100 CBM-20A/20Alite 5-107...
  • Page 236 Setting range Minimum unit Default setting Remark 25, 80, 160, 240, Sampling Rate (msec) 320, 640, 1280, 2000 Slit Width 1.2, 8 Reference Compensation On, Off Reference Wavelength 190 to 800 (nm) Reference Bandwidth (nm) 1 to 50 5-108 CBM-20A/20Alite...
  • Page 237 Common parameters Slope (µV/sec) 0 to 1000000 -4500 to Level (µV) -4500 1000000 Width (sec) 1 to 99 Auto Delay On, Off 0.100 to Flow Rate (mL/min) 0.001 1.000 150.000 Detector Time Constant 0.00 to 20.00 0.01 1.00 (sec) CBM-20A/20Alite 5-109...
  • Page 238 No.] is the last number which was set in the "Fraction Collector Rack Adjustment" window. 2. The [End Vial No.] for User Rack 9 is the last number which was set in the "Fraction Collector Rack Adjustment" window. 5-110 CBM-20A/20Alite...
  • Page 239 5.12 Parameter Details 5.12.6 System Controller  Method Parameters for the System Controller CBM-20A  Setting Default Parameter Remark range setting Event Output: Event output is controlled by turning the 4 (2 with Event1 CBM-20-Alite) relay contact outputs ON and OFF.
  • Page 240 ROM BASE II. Reserved: Reserved for future expansion. Do not use this setting. Opt-Link(PAC): Connects to Chromatopac via optical cable. Connect to remote connector 8 Opt- on the CBM-20A or remote connector 4 on Link(PAC) Interface Ethernet the CBM-20Alite. RS-232C...
  • Page 241 0.0 to 130.0 No checking when 0 is set. Enabled when "MANUAL" is selected for 0 to 1326 kgf/cm [Enable System P.Max]. When "AUTO" is 0 to 19000 selected, the system P.Max value automatically recognized is displayed. 0 to 1300 CBM-20A/20Alite 5-113...
  • Page 242 P.Max values for respective models. If no autosampler is connected, the system P.Max value defaults to 0. Model name System P.Max value (MPa) SIL-30ACMP 130.0 SIL-30AC SIL-20AXR 66.0 SIL-20ACXR SIL-20AHT UFLC SIL-20ACHT UFLC 35.0 SIL-20AHT SIL-20ACHT SIL-20A 20.0 SIL-20AC SIL-10ADvp SIL-10A 34.3 SIL-10AF SIL-10AP SIL-10Ai 19.6 Not connected 5-114 CBM-20A/20Alite...
  • Page 243 Total flow rate for T.FLOW gradient pumping 0 to 10.0000 0.0001 (See note 3.) (mL/min) B.CONC Concentration of T.GE1 0 to 100 solvent B (C) (%) C.CONC B.CURV Gradient curve profile 10 to 10 of solvent B (C) C.CURV CBM-20A/20Alite 5-115...
  • Page 244 Number of FCV-10AL- 1 to 4 type solenoid valve For example, set Number of FCV-11AL- [13] to turn ON 0 to 123 type solenoid valve solenoid valves 1 and 3. 1. This function is not available in fast LC mode. 5-116 CBM-20A/20Alite...
  • Page 245 B and C Number of FCV-10AL- 1 to 4 type solenoid valve PASV For example, set PBSV Number of FCV-11AL- [13] to turn ON PCSV 0 to 123 type solenoid valve solenoid valves 1 and 3. CBM-20A/20Alite 5-117...
  • Page 246 B.GE1, B.GE3 of solvent B Number of FCV-10AL- 1 to 4 type solenoid valve PASV PBSV For example, set PCSV Number of FCV-11AL- [13] to turn ON 0 to 123 PDSV type solenoid valve solenoid valves 1 and 3. 5-118 CBM-20A/20Alite...
  • Page 247 Flow rate (mL/min) 0 to 10.000 0.001 Number of FCV-10AL- 1 to 4 type solenoid valve ISO1 to ISO4 For example, set Number of FCV-11AL- [13] to turn ON 0 to 123 type solenoid valve solenoid valves 1 and 3. CBM-20A/20Alite 5-119...
  • Page 248 Flow rate (mL/min) 0 to 9.999 0.001 Number of FCV-10AL- 1 to 4 type solenoid valve ISO1 to ISO4 For example, set Number of FCV-11AL- [13] to turn ON 0 to 123 type solenoid valve solenoid valves 1 and 3. 5-120 CBM-20A/20Alite...
  • Page 249 T.FLOW min) for gradient 0 to 9.999 0.001 pumping B.CONC Concentration of LP.GE1 C.CONC solvents B, C, and D 0 to 100 D.CONC B.CURV Gradient curve profile 10 to 10 C.CURV of solvents B, C and D D.CURV CBM-20A/20Alite 5-121...
  • Page 250 B and C Number of FCV-10AL- 1 to 4 type solenoid valve PASV For example, set PBSV Number of FCV-11AL- [13] to turn ON PCSV 0 to 123 type solenoid valve solenoid valves 1 and 3. 5-122 CBM-20A/20Alite...
  • Page 251 1 to 4 valve PASV For example, set PBSV Number of FCV-11AL [13] to turn ON 0 to 123 type solenoid valve solenoid valves 1 PCSV and 3. PDSV Number of FCV- 230AL type solenoid 0 to 3 valve CBM-20A/20Alite 5-123...
  • Page 252 B, C, and D 0 to 100 (See note 1.) D.CONC B.CURV Gradient curve profile 10 to 10 C.CURV of solvent B, C, and D D.CURV 1. The LP.GE mode is not available on the LC-20AP earlier than version 1.10. 5-124 CBM-20A/20Alite...
  • Page 253 0 to 3 valve Total flow rate for T.FLOW gradient pumping 0 to 20.000 0.001 (mL/min) B.CONC Concentration of T.GE1 0 to 100 solvent B (C) (%) C.CONC B.CURV Gradient curve profile -10 to 10 of solvent B (C) C.CURV CBM-20A/20Alite 5-125...
  • Page 254 B.CONC Concentration of 0 to 100 C.CONC solvents B and C (%) T.GE1 B.CURV Gradient curve profile 10 to 10 C.CURV of solvents B and C PASV Number of FCV- PBSV 130AL-type solenoid 0 to 2 PCSV valve 5-126 CBM-20A/20Alite...
  • Page 255 B.CONC Concentration of 0 to100 C.CONC solvents B and C (%) T.GE1 B.CURV Gradient curve profile 10 to 10 C.CURV of solvents B and C PASV Number of FCV-7AL- PBSV 0 to 1 type solenoid valve PCSV CBM-20A/20Alite 5-127...
  • Page 256 0, 1 valve on L side OvenA RV.L OvenB Position of 6-position 1 to 6 valve on L side Position of 2-position 0, 1 valve on R side RV.R Position of 6-position 1 to 6 valve on R side 5-128 CBM-20A/20Alite...
  • Page 257 R side RV.R Position of 6-position 1 to 6 valve on R side  CTO-10Avp/10ACvp/10ASvp/10A/10AC Setting Minimum Default Module Function Description Remark range unit setting OvenA Oven temperature OVEN.T 0, 4 to 80 0 to OFF OvenB (ºC) CBM-20A/20Alite 5-129...
  • Page 258 Wavelength 1 (nm) single mode DetectorB 701 to 900 371 to 700 Settings for W lamp in dual mode 701 to 900 190 to 370 Settings for D2 371 to 700 and W lamps in dual mode 701 to 900 5-130 CBM-20A/20Alite...
  • Page 259 Wavelength 1 (nm) 190 to 370 Settings for dual mode 371 to 600 190 to 370 DetectorA WAVE2 Wavelength 2 (nm) 371 to 600 DetectorB RESP Response 1 to 10 0:OFF LAMP Lamp selection 1:D2 Execution of auto- ZERO zero CBM-20A/20Alite 5-131...
  • Page 260 Setting for D2 190 to 370 lamp DetectorA WAVE2 Wavelength 2 (nm) DetectorB 371 to 700 Settings for W lamp 701 to 900 RESP Response 1 to 10 0:OFF LAMP Lamp selection 1:D2 Execution of auto- ZERO zero 5-132 CBM-20A/20Alite...
  • Page 261 3:LOW 0:OFF LAMP Lamp selection 1:ON CELL.T Cell temperature (ºC) 0, 4 to 40 0 to OFF (See note 1.) Marker output to MARK recorder terminal Execution of auto- ZERO zero 1. Cannot be used with the RF-20A. CBM-20A/20Alite 5-133...
  • Page 262 DetectorB 1:x1 GAIN Gain 2:x4 3:x16 With the RF-10A, 1:HIGH available settings SENSE Sensitivity 2:MID are 1:HIGH and 3:LOW 2:LOW. 0:OFF LAMP Lamp selection 1:ON Marker output to MARK recorder terminal DetectorA DetectorB Execution of auto- ZERO zero 5-134 CBM-20A/20Alite...
  • Page 263 Setting for solvent SV.LEVEL SV Level (µRIU) 0 to 9999.00 0.01 9999.00 recycle valve Execution of PURGE reference purge Stoppage of reference PURGE.E purge Automatic adjustment BALANCE of optical balance Marker output to MARK recorder terminal ZERO Execution of autozero CBM-20A/20Alite 5-135...
  • Page 264 CELL.T Cell temperature (ºC) 0 to OFF DetectorB 30.0 to 60.0 Execution of R.FLOW reference purge Stoppage of reference R.CLOSE purge Automatic adjustment BALANCE of optical balance Marker output to MARK recorder terminal Execution of auto- ZERO zero 5-136 CBM-20A/20Alite...
  • Page 265 (See note 1.) (Rack9) (Rack1) 0 to 143 (Rack2) 0 to 63 (Rack3) 0 to 15 Number of last vial for F.VIAL (Rack4) fractionation 0 to 49 (Rack5) (Rack6 to 8) (See 0 to note 1.) (See note 1.) CBM-20A/20Alite 5-137...
  • Page 266 (Rack6 to 8) 0 to (See note 1.) (Rack9) Not available Stops fractionation performed forcibly in accordance with the GOTO.V command RET.V and moves the nozzle to the vial where it was before execution of the GOTO.V command. 5-138 CBM-20A/20Alite...
  • Page 267 Relay output to OUT [13] to turn ON EVENT 0 to 1234 terminal event outputs 1 and 3. Control of multiple 0:OFF, Controller POWER CBM-20A only terminal box 1:ON Output of analysis START start signal STOP Stops time program  SUBC-vp/OPTION-BOX vp Setting...
  • Page 268 Description Remark range unit setting RV.A Position of valve A 0 to 1 RV.B Position of valve B 0 to 1 SubcB RV.C Position of valve C 1 to 6 RV.D Position of valve D 1 to 6 5-140 CBM-20A/20Alite...
  • Page 269 [None] in the [External Start Output] field in the "Configuration Parameter" window to stop the start signal being output when time program starts. Also, set the START command to be executed at the same time as the INJECT command in the time program. "START Command" P. 5-143 CBM-20A/20Alite 5-141...
  • Page 270 Status of CBM Completion of previous analysis Data processing Data processing Data collection in READY status S.PRET command CBM's time program 10.0 Example of Time Program Autosampler S.PRET 10.0 Controller STOP Fig. 5.3 Analysis Using the S.PRET Command 5-142 CBM-20A/20Alite...
  • Page 271 Example of Time-program setting: Time Module Function Value Description 0.01 B.GE1 B.CONC Initial concentration 0.02 B.GE1 B.CURV CURV should be set in the next line of CONC. B.GE1 B.CONC 80.0 B.GE1 B.CONC 80.0 15.01 B.GE1 B.CONC Controller STOP CBM-20A/20Alite 5-143...
  • Page 272 Gradient Curve Profile  The details of the gradient curve profile are as follows:       When a < > 0         When a = 0   5-144 CBM-20A/20Alite...
  • Page 273 The fast LC mode is enabled only on systems where solvent delivery pumps are controlled from a CBM-20A controller, pump A is an LC-20AB unit or both pumps A and B are any of LC- 30AD, LC-20AD or LC-20ADXR unit, operating in the B.GE mode. All other configurations will only operate in the normal mode, even if set to the fast LC mode.
  • Page 274 When using an SPD-M20A/M30A detector for fast analysis, make sure the system is configured as follows. • CBM-20A version 1.20 or later is combined with SPD-M20A version 1.07 or later or SPD- M30A. • An autosampler is connected •...
  • Page 275 Note that the number for each plate position may vary depending on the settings on the autosampler. For details, refer to the SIL-30ACMP instruction manual. When specifying the control vial rack, set 0 in the [Rack No.] column in the sequence table. CBM-20A/20Alite 5-147...
  • Page 276 Set 0 in the [Rack No.] column in the sequence table when specifying the control vial rack. Set 1 to 12 in the [Rack No.] column in the sequence table when specifying a rack in the rack changer. 5-148 CBM-20A/20Alite...
  • Page 277 Rack Sample No. Rack S 0 to 99 Rack L 0 to 79 Cooling rack S 0 to 59 Cooling rack L 0 to 49 Rack LL 0 to 24 MTP1 1 to 96 MTP2 1 to 192 CBM-20A/20Alite 5-149...
  • Page 278 Column Name and Column Size are displayed in Name (Size) format in the first line, and Mobile Phase A to D are displayed in A/B/C/D format in the second line. (If the character string exceeds the column width, the excess characters will not be displayed. 5-150 CBM-20A/20Alite...
  • Page 279 Troubleshooting Contents Troubleshooting and Corrective Action ............6-2 Error Message ....................6-6...
  • Page 280 For more detailed procedures, refer to the indicated page. If the problem cannot be resolved even after taking the indicated measures, or if there are problems not included in the following tables, contact your Shimadzu representative.  System Configuration...
  • Page 281 Verify that the configuration parameters are set correctly for the properly. system controller. The detector is not auto-zeroed "5.12 Parameter Details" P. 5-57 when analysis starts. The Chromatopac does not start when analysis starts. CBM-20A/20Alite...
  • Page 282 "Print" window is displayed. system configuration cannot be "2.9 Precautions Related to Internet Explorer Operations" printed. P. 2-32 Check the Internet Explorer settings. The windows are not displayed properly. "2.9 Precautions Related to Internet Explorer Operations" Internet Explorer doesn’t start. P. 2-32 CBM-20A/20Alite...
  • Page 283 "Configuration" with different version numbers, where the CBM connected to tab page. the network is changed by turning ON/OFF the power to the system controllers. • Changes where the version of the CBM program becomes lower. CBM-20A/20Alite...
  • Page 284 6-7. Method and sequence files contain the following data. • Method-file ("Method00" to "Method19") parameters and time programs • Sequence-file ("Sequence00" to "Sequence11") sequences and standard-sample injection tables • Shutdown-file ("ShutDown00" to "ShutDown11") and startup-file ("StartUp00" to "StartUp11") settings CBM-20A/20Alite...
  • Page 285 In the case of the Nexera UC series instruments (LC-30ADSF, SFE-30A, and SFC- 30A), operations using Internet Explorer are not guaranteed and no error message will be displayed.  Error Window CBM-20A/20Alite...
  • Page 286 Rack on rack plate L is not adjusted. 0x8542 Rack on rack plate M is not adjusted. Autosampler 0x8543 Rack on rack plate R is not adjusted. 0x8544 Invalid rack ID. 0x8545 Racks with same rack ID are set. CBM-20A/20Alite...
  • Page 287 Detector 0x0050 No scan data 0x87D0 Low set temperature. 0x8721 Low set temperature. 0x8952 LAN timeout error has occurred. (PC) 0x8954 LAN timeout error has occurred. (CBM) PDA Detector 0x89D2 Temperature is increasing inside the instrument. 0x89FB Undefined warning. CBM-20A/20Alite...
  • Page 288 [Clear (Next error)] is pressed. If an error occurs, the currently executed time program stops, and the operation of autosamplers and fraction collectors is interrupted. Also, operation according to the operation level is performed as described in the following table. 6-10 CBM-20A/20Alite...
  • Page 289 Invalid injection volume. 0x8531 Pretreatment file does not exist. The sequence has skipped because a rack is not 0x8540 adjusted. Adjust the rack. 0x8549 Invalid vial number. Leak error is detected by a sensor near a Low- 0x8550 pressure valve. CBM-20A/20Alite 6-11...
  • Page 290 0x8718 Invalid wavelength calibration data. 0x8719 Hg lamp does not light. 0x871A The wavelength shift is greater than 2 nm. 0x871B The wavelength shift is greater than 2 nm. 0x871C The wavelength shift is greater than 2 nm. 6-12 CBM-20A/20Alite...
  • Page 291 The exposure time of the installed cell is not 0x8966 adjusted. Set the exposure time. The wavelength of the installed cell is not 0x8967 calibrated. Perform wavelength calibration. 0x8971 Invalid slit position. 0x8972 Invalid response. 0x8973 Invalid sampling rate. 0x8974 Invalid cell temperature. CBM-20A/20Alite 6-13...
  • Page 292 Undefined error (Level 2). 0x89FE Undefined error (Level 3). 0x000A Link time out error. 0x0024 User rack is not adjusted. Configure it and activate. Fraction collectors 0x8F01 No empty vial in the fraction collector. 0x8F02 Liquid leakage has been detected. 6-14 CBM-20A/20Alite...
  • Page 293 LAN timeout error has occurred (PDA Detector). 0x856A Invalid injection volume. (Autosampler) 0x000A Link time out error. Syringe Unit: The setting for syringe aspiration/ Sub controller 0x0086 discharge is out of range. 0x008C Leak error is detected. Supercritical 0x8BB0 Leak is detected. controller CBM-20A/20Alite 6-15...
  • Page 294 0x81E2 Abnormal high temperature is detected inside the instrument. 0x81E3 Motor cooling fan has stalled. Pump 0x81E5 Leak sensor is open or short-circuit. 0x81E6 Motor current error is detected. 0x81F0 Communication error occurred between A and B pumps. 6-16 CBM-20A/20Alite...
  • Page 295 Rack-changer malfunction in Y (front-back) direction. 0x85D3 Rack-changer home position error in X (left-right) direction. 0x85D4 Rack-changer home position error in R (rotation) direction. 0x85D5 Rack-changer home position error in Z (up-down) direction. 0x85D6 Rack-changer home position error in Y (front-back) direction. CBM-20A/20Alite 6-17...
  • Page 296 Extraction vessel temperature sensor is open or short-circuit. 0x8B44 Pre-heater temperature sensor is open or short-circuit. 0x8B47 The extraction vessel heater failure. 0x8B48 Pre-heater failure. 0x8B49 The extraction vessel temperature sensor wire is broken. 0x8B50 Room temperature sensor is open or short-circuit. 6-18 CBM-20A/20Alite...
  • Page 297 Error in the flow cell's heating unit. 0x8725 Leak sensor is open. 0x8726 Leak sensor is short. 0x8728 Error in the temperature sensor. 0x8729 Error in hardware. 0x8961 The cell is overheated. PDA detector 0x8962 The cell overcurrent has occurred. CBM-20A/20Alite 6-19...
  • Page 298 Pump head temperature sensor is open or short-circuit. 0x8BA1 Pump head cooling fan rotation error. 0x8BA2 Coolant pump for cooling the pump head failure. 0x8BB1 Leak sensor is open or short-circuit. All instruments (Common error 0x2E00 Undefined fatal error. code) 6-20 CBM-20A/20Alite...
  • Page 299 Hardware Validation This chapter provides instruction on hardware validation, which verifies the performance of individual components and the instrument as a whole. Contents Overview of Hardware Validation ..............7-2 Implementation of Hardware Validation ............7-3 Validation Precautions ..................7-4 Equipment Required for Validation ..............7-6 Validation: System Controller ................

  • Page 300: Overview Of Hardware Validation

    The operational protocol and criteria for this component and the HPLC system are described in this chapter to assist the user in conducting validation. Refer to each the instruction manual for each component for operational protocol of that specific component. CBM-20A/20Alite...

  • Page 301: Implementation Of Hardware Validation

    After any maintenance, component performance must be re-validated. The type of validation depends on the actual work done. If the maintenance inspection cannot be performed solely by the specific component validation, system validation is required. Maintenance information and results of hardware validation must be recorded and kept for future reference. CBM-20A/20Alite...

  • Page 302: Validation Precautions

    When flammable solvents are used, open flame or other fire sources must be strictly prohibited. • Precautions for use of the low-pressure Hg (Mercury) lamp Install the low-pressure Hg (Mercury) lamp before turning on the power of the lamp. Looking straight at the lamplight could damage eyes. CBM-20A/20Alite...
  • Page 303 • Provide adequate installation surface and space The weight of CBM-20A is 5.5 kg. During installation, consider the entire weight combined with other LC components. The lab table on which this instrument is installed should be strong enough to support the total weight of the LC system.
  • Page 304 For inspection of the flow rate accuracy for the solvent delivery module. Measuring flask Obtain a 5 mL-measuring flask. For inspection of the injection volume accuracy for the autosampler. Electronic balance The balance must be calibrated and able to perform measurement with a 0.001g precision at the time of inspection. CBM-20A/20Alite...

  • Page 305: Equipment Required For Validation

    Naphthalene (60 mg/ For inspection of system reproducibility for a system S228-32996-01 equipped with a spectrofluorometric detector. Glycerol (0.872 mg/L) S228-32996-05 For inspection of the span for the refractive index detector. CBM-20A/20Alite...
  • Page 306 For inspection of the wavelength accuracy for the PTFE block assembly S228-34319-91 spectrofluorometric detector. Column Particle size: 5µm Shim-pack VP-ODS S228-34937-91 Column Dimension: I.D. 4.6 mm × length 150 mm (An equivalent ODS column may also be used.) LUNA C18(2) 00F-4252-E0 For the system validation. CBM-20A/20Alite...

  • Page 307: Validation: System Controller

    If the memory check is completed normally, the system starts up. Start up Internet Explorer at the PC and enter the URL for the system controller. Check the version number (version number of the contents) displayed together with the Shimadzu logo. Log into the system controller. CBM-20A/20Alite...
  • Page 308 Set the flow rate for LP.GE1 to 1 mL/min and concentration B to 50 %. Click in the "Analysis" tab page. High-pressure gradient system Set the flow rate for B.GE1 to 1 mL/min and concentration B to 50 %. 7-10 CBM-20A/20Alite...
  • Page 309 Start up the system controller and Internet Explorer. Check that the analysis program for the previous test is recorded. After completing inspection, return all the conditions to their original state. • CHECK • All the input parameters must be saved in memory. CRITERIA CBM-20A/20Alite 7-11...

  • Page 310: System Validation

    Both the water (distilled) and the methanol should be HPLC grade Shim-pack VP-ODS (Part No. S228-34937-91), Column LUNA C18 (2) (Part No. 00F-4252-E0) or equivalent ODS column (Particle size 5 µm, Column Dimension : I.D. 4.6 mm × length 150 mm) 7-12 CBM-20A/20Alite...
  • Page 311 (methanol, acetonitrile, etc.) > Clean the flow lines with water. Pass water through the flow lines for 10 minutes, at a rate of 2 mL/min. CBM-20A/20Alite 7-13...
  • Page 312 Detector Autosampler Coupling 1.6C Fig. 7.1 When cleaning is finished, pour mobile phase (mixture of water and methanol (3/2, (v/v)) into the reservoir, and reconnect the column with the LC system. Detector Autosampler Tubing Column Tubing Fig. 7.2 7-14 CBM-20A/20Alite...
  • Page 313 Rinse Speed 35 µL/sec Rinse Mode No Rinse Column oven Oven Temperature 40 ºC Wavelength 272 nm Detector AUX Range 1 AU/V Response 0.5 sec For details on setting component parameters, refer to "4.2 Creating Method Files" P. 4-10. CBM-20A/20Alite 7-15...
  • Page 314 Injection Volume 10µL Injections/Vial 6 times Run Time 5 min For details on creating sequence tables, refer to "4.3 Creating Sequence Files" P. 4-22. When using a manual injector, inject a volume equal to 5 times the loop volume. 7-16 CBM-20A/20Alite...
  • Page 315 Both the water (distilled) and the methanol should be HPLC grade. Shim-pack VP-ODS (Part No. S228-34937-91), Column LUNA C18 (2) (Part No. 00F-4252-E0) or equivalent ODS column (Particle size 5 µm, Column Dimension : I.D. 4.6 mm × length 150 mm) CBM-20A/20Alite 7-17...
  • Page 316 (methanol, acetonitrile, etc.) > Clean the flow lines with water. Pass water through the flow lines for 10 minutes, at a rate of 2 mL/min. 7-18 CBM-20A/20Alite...
  • Page 317 7.6 System Validation Detector Autosampler Coupling 1.6C Fig. 7.3 When cleaning is finished, pour mobile phase (A: water, B: methanol) into the reservoir, and reconnect the column with the LC system. Detector Autosampler Tubing Column Tubing Fig. 7.4 CBM-20A/20Alite 7-19...
  • Page 318 [ISO/Binary Pump] is selected for the LC-20AB Solvent Delivery Module and [B.GE] is selected for other solvent delivery modules. For details on the display of the "Configuration" tab page, refer to "3.4 Determine the System Configuration" P. 3-7. 7-20 CBM-20A/20Alite...
  • Page 319 Verify that liquid flows through the detector outlet tubing, and that there are no leaks from any of the connections. Monitor the baseline. When the baseline has stabilized, click CBM-20A/20Alite 7-21...
  • Page 320 : Relative standard deviation C.V. : Coefficient of variation • CHECK • The RSD (C.V.)'s obtained must satisfy the following CRITERIA criteria: • Retention time RSD must not exceed 0.5 %. • Peak area RSD must not exceed 1.0 %. 7-22 CBM-20A/20Alite...

  • Page 321: If Validation Fails

    For troubleshooting procedures for individual system components, see the applicable instruction manuals. • If a cause cannot be determined, contact your Shimadzu representative : If you are unable to determine the cause of the failure, or if you are unclear about troubleshooting or corrective action procedures, contact your Shimadzu representative.
  • Page 322 7. Hardware Validation This page is intentionally left blank. 7-24 CBM-20A/20Alite...
  • Page 323 Maintenance The hardware status for the whole system and for each component can be checked with the system controller. It is also possible to obtain maintenance information that can be used as a guide for timing the replacement of parts. This chapter explains how to perform system checks as well as how to obtain maintenance information.
  • Page 324 System checks are executed from the "System Check" tab page in the "Status Summary" section. Click the [System Check] tab in the "Status Summary" section. The "System Check" tab page is displayed. Click the name (or ) of the system to be checked. The "Login" window is displayed. CBM-20A/20Alite...
  • Page 325 These items are not checked as part of the [Normal] system check. Wavelength accuracy check cannot be executed with the SPD-M20A/M30A from the CBM-20A, but data of the SPD-M20A/M30A can be collected and check results displayed. System checks can be executed for all LC-30A/LC-20A/LC-10Avp-series components (except LC-10Avp-series Sub-controllers).
  • Page 326 Result for the LC system. Displayed if the system passed the check. Displayed if there is a replaceable part that has passed its recommended replacement date. Displayed if the system failed the check. Displayed if the system check has not been executed. CBM-20A/20Alite...
  • Page 327 Displays the version numbers of the connected components. Serial No. Displays the serial numbers of the connected components. Displays the system-check results for the connected components. Result The meanings of the displayed symbols are as previously described. [-] is displayed if there is no check item. CBM-20A/20Alite...
  • Page 328 When the power is turned OFF, only the check results for the system as a whole are saved, and the data for the "Summary Result" table and the "Detailed Results" table is lost. CBM-20A/20Alite...
  • Page 329 Do not click . Clicking this button and associating the file with another application may make it impossible to save the file. If this button is inadvertently clicked and the "Open With" window is displayed, close the window by clicking [Cancel]. CBM-20A/20Alite...
  • Page 330 8. Maintenance Click The following window is displayed. Select the save location. Input the filename. Click The file is saved in the selected location and the following window is displayed. Click in the "Download Complete" window. The window is closed. CBM-20A/20Alite...
  • Page 331 For details, refer to "8.2.3 List of Displayed Maintenance Items". Display item Description Unit Name Displays the names of the components connected to the system. Unit Model Displays the model names of the connected components. Item Displays the maintenance items. CBM-20A/20Alite...
  • Page 332 L SEAL [L] Displayed Displayed LC-20AP R SEAL [L] LC-20AR Solvent LC-20Ai delivery module LC-10ADvp L SEAL [L] Displayed Not displayed R SEAL [L] LC-10ATvp LC-10AD LC-10AT LC-10AS LC-10Ai TOTAL REV [rev] Not displayed Not displayed LC-6AD LC-7A LC-8A 8-10 CBM-20A/20Alite...
  • Page 333 W TIME [hr] Detector SPD-10A connected D2 TIME [hr] 2000 Not displayed SPD-10Ai SPD-10AV D2 TIME [hr] 2000 Not displayed SPD-10AVi W TIME [hr] 2000 SPD-M30A D2 TIME [hr] Displayed Displayed D2 TIME [hr] SPD-M20A Displayed Displayed W TIME [hr] CBM-20A/20Alite 8-11...
  • Page 334 Present value item with the mouse to reset it. Enter the password according to the instructions displayed on screen. 2: If using an SIL-10AXL, the recommended value for the NEEDLE SEAL item is 40000. 3: If the syringe volume is 2500 or 5000 µL, the recommended values are respectively 22500 and 45000. 8-12 CBM-20A/20Alite...
  • Page 335 8.3.1 Periodic Inspection and Maintenance It is necessary to perform periodic inspections of this instrument to ensure its safe use. It is possible to have these periodic inspections performed by Shimadzu service representatives on a contractual basis. For information regarding the maintenance inspection contract, contact your Shimadzu representative.
  • Page 336 8. Maintenance This page is intentionally left blank. 8-14 CBM-20A/20Alite...
  • Page 337 Technical Information This chapter provides technical information about the system controller such as, installation location, installation conditions, plumbing, wiring, specifications and methods for connecting to other devices. Contents Installation ....................... 9-2 Connecting the System Controller and Components ........9-7 Initializing Components ................. 9-29 System Controller Installation and Setup (Example) ........

  • Page 338: Installation

    • air currents from heating or air conditioning equipment are not directed on the instrument. • sunlight does not shine directly on the instrument. • there is no vibration. • humidity stays within 20 - 85 %. • place without condensation CBM-20A/20Alite...
  • Page 339 Required Installation Space CAUTION • The weight of CBM-20A is 5.5 kg. During installation, consider the entire weight combined with other LC components. The lab table on which this instrument is installed should be strong enough to support the total weight of the LC system.
  • Page 340 9. Technical Information 9.1.2 Installation  Installation The instrument is designed for stacking with other Shimadzu HPLC components. "9.7 Introduction to HPLC System" P. 9-53 CAUTION When the LC-20A series components are stacked on each other, the clearance between the components is only 5 mm.
  • Page 341 • Do not bend the cord excessively or pull on it. • To unplug the instrument, pull the plug itself, NOT the cord. If the cord is damaged, replace it immediately. CAUTION Before plugging in the instrument, make sure that the power switch is OFF. CBM-20A/20Alite...
  • Page 342 To prevent electric shock and to maintain stability in operation of the product, be sure to ground the product. The product will be grounded when the provided 3-wired power plug is inserted into a 3- wired power socket equipped with a ground terminal. CBM-20A/20Alite...

  • Page 343: Connecting The System Controller And Components

    Failure to observe the above cautions could result in fire, electric shock or instrument malfunction. 9.2.1 Types of Connector The different types of connectors on the back of the system controller and the components that are connected to them described below.  CBM-20A Channel 1 Remote Channel 2 connectors Channels 3 to 8...
  • Page 344 "9.2.9 Chromatopac Connection and Setup" P. 9-21. Used to connect external devices. External input/output terminals "External Input/Output Terminals (CBM-20A)" P. 9-9 Used to connect Chromatopac or a PC. RS-232C connector "9.2.9 Chromatopac Connection and Setup" P. 9-21 "9.2.10 (RS-232C) LC Workstation Connection and Setup" P. 9-25 Ethernet connector Used to connect a PC via a network.
  • Page 345 9.2 Connecting the System Controller and Components External Input/Output Terminals (CBM-20A)  Fig. 9.7 Name Description Contact input terminals used to start analysis. They can be External start input connected to a manual injector. terminals (MAN.INJ.) "9.2.11 External Device Connection and Setup" P. 9-27 Contact input terminals used for alarm signals.
  • Page 346 Enables channels 9 to 12 to be used. Board Board for analog-digital conversion for connecting a detector that PC-55N A/D Board uses the analog output. Optional boards are mounted by Shimadzu service personnel. Consult your Shimadzu representative for details. 9-10 CBM-20A/20Alite...
  • Page 347 "External Input/Output Terminals (CBM-20Alite)" P. 9-12 Used to connect Chromatopac or a PC. RS-232C connector "9.2.9 Chromatopac Connection and Setup" P. 9-21 "9.2.10 (RS-232C) LC Workstation Connection and Setup" P. 9-25 Ethernet connector Used to connect a PC via a network. (ETHERNET) CBM-20A/20Alite 9-11...
  • Page 348 Error input terminals Contact input terminals used for alarm signals. They can be connected (IN1) to a degassing unit. General-purpose output Contact output terminals used for signals related to starting and terminals stopping analysis, errors, and events. (OUT1/2) 9-12 CBM-20A/20Alite...
  • Page 349 When a cap is removed, keep it in a safe place for future use. Insert the optical cable plug into the [REMOTE] connector until it clicks into place. Blue Gray Plug Insert until it clicks. Fig. 9.12 CBM-20A/20Alite 9-13...
  • Page 350  Installation and Setup Installation  The CBM-20Alite is installed by Shimadzu service personnel. Setup  Set [0] (Int) for the [CBM LINK] parameter in the [System Settings Group] for the autosampler or solvent delivery module in which the CBM-20Alite is incorporated.
  • Page 351 Insert one end of the optical cable provided with the autosampler into the remote connector (REMOTE) on the autosampler. Insert the other end of the cable into the remote connector for channel 1 on the system controller. CBM-20A SIL-20A Optical cable Fig. 9.14 The autosampler is always connected to channel 1.
  • Page 352 Connect the solvent delivery pump, column oven, detector or supercritical controller to channels 1 to 8 of the remote connector. If more than eight channels are necessary on the CBM-20A, add an optical connector expansion board (CBM OPT-4) option to enable the use of up to channel 12.
  • Page 353 8 on the system controller. (Channel1 to 12 if an optional optical-connector expansion board is mounted.) Make a note of the channel number used for connection. Set the channel number at the component. CBM-20A CBM-20A LC-20AT CTO-20A Optical cable...
  • Page 354 If using an LC-30A/20A series pump (LC-30AD/20AB/20AD/20AT/20AP/20AR, etc.), verify that the [CBM LINK] value is [1] (Ext). If the [LOCAL] value is [1], the component is in local mode and control will not be possible from the system controller. 9-18 CBM-20A/20Alite...
  • Page 355 9.2 Connecting the System Controller and Components 9.2.7 Sub-controller Connection and Setup It is possible to control a solenoid valve or degassing unit from the CBM-20A by connecting a sub-controller vp, Option Box vp, Option Box L or Valve Interface. Up to 2 of these components can be controlled from the CBM-20A.
  • Page 356 Insert the other end of the optical cable into the remote connector on the system controller. Use the channel number set with the DIP switch. Connection is performed in the same way for Option Box vp, Option Box L, and Valve Interface. 9-20 CBM-20A/20Alite...
  • Page 357 9.2 Connecting the System Controller and Components 9.2.8 Connecting the Multiple Terminal Box If the CBM-20A is connected to a multiple terminal box, 4 of the 8 power outlets can be controlled with the system controller. There are two types of multiple terminal box: one type is used by mounting onto the back of Option Box L and the other is used as an individual unit.
  • Page 358 Insert the other end of the optical cable into the optical-link connector (PAC) on the system controller. The remote connectors for channel 8 on the CBM-20A and channel 4 on the CBM- 20Alite also act as optical-link connectors. The system controller and Chromatopac can also be connected using the RS- 232C connectors.
  • Page 359 Select Opt-Link (PAC) if the Chromatopac is connected via an optical-link connector (OPT LINK). Select RS-232C if the Chromatopac is connected via the RS-232C connector. Enter the channel number for the Chromatopac in the [Chromatopac Channel] field under [Chromatopac Setting]. Click to save the settings. CBM-20A/20Alite 9-23...
  • Page 360 The communication port should be closed before turning OFF the power to the system controller or Chromatopac. If it was opened using [OPEN GCLC 7,1,1], enter [CLOSE GCLC 1] and press . If it was opened using [OPEN TRS 7], enter [CLOSE TRS 7] and press 9-24 CBM-20A/20Alite...
  • Page 361 LC Workstation (LCsolution or Class-VP) via a network (ETHERNET) or an RS- 232C cable.  Connecting via a Network Connect the system controller and the PC to the same network.  Connecting via RS-232C Connect an RS-232C cable to the RS-232C connector on the system controller and the PC. CBM-20A/20Alite 9-25...
  • Page 362 Select the interface from the [Interface] pull-down menu. Select [Ethernet] if LC Workstation is connected via a network (ETHERNET). Select [RS-232C] if LC Workstation is connected using RS-232C connectors. Click to save the settings. Close Internet Explorer. Reset the power to the system controller. 9-26 CBM-20A/20Alite...
  • Page 363 The external input/output terminals of the system controller are used to connect to external devices other than LC-30A/20A/LC-10Avp/LC-10A-series components and control them from the system controller. The names and connection methods of the external input/output terminals are given below. Fig. 9.17 CBM-20A/20Alite 9-27...
  • Page 364 Cable with single wire :  0.4 to  1.2 (AWG26 to 16) • • Cable with stranded wire : 0.3 mm to 1.25 mm (AWG22 to 16), diameter of single wire thicker than  0.18. The cable with stranded wire is suitable to prevent disconnection. 9-28 CBM-20A/20Alite...

  • Page 365: Initializing Components

    IP address 192.168.200.99 Subnet mask 255.255.255.0 Default gateway None Group Name Unregistered System Name Serial number User name and level Administrator User ID Admin Password Admin For details on default settings, refer to "5.12 Parameter Details" P. 5-57. CBM-20A/20Alite 9-29...
  • Page 366 When initialization is complete, the 100M, ACT, and LINK indicators on the back of the system controller all turn ON briefly and then turn OFF and return to the normal status The status indicator of the CBM-20A changes from orange to green. 9.3.2 Making TCP/IP-related Settings The [IP address], [Subnet mask], [Default gateway], and [Ether speed] parameters can be set via the CBM-20A's RS-232C terminals.
  • Page 367 2. Subnet mask Sets the default gateway. 3. Default gateway If there is no default gateway, input [-]. Sets the Ethernet speed/mode for the CBM-20A. Any value in the range 0 to 4 can be set. 0 : Auto 1 : 10M/Half 2 : 10M/Full 4.

  • Page 368: System Controller Installation And Setup (Example)

    The following example describes how to set group names and IP addresses. 9.4.1 System Configuration (Example) The following example describes connecting this type of LC system to the network. Group 1 Group 2 Group N Group 1 Fig. 9.18 9-32 CBM-20A/20Alite...
  • Page 369 LC systems makes it possible to handle them as systems in the same group. (One group can contain a combined total of up to 10 CBM-20A/20Alite System Controllers and SPD-M20A/M30A PDA Detectors.) User IDs and user levels can be copied from one system controller to another system controller in the same group.
  • Page 370 The IP address, if known, it can be changed by connecting directly to a PC and changing the setting from Internet Explorer. It is possible to set the IP address and related settings by temporarily connecting an LC-30A/20A-series component (solvent delivery module, autosampler, or UV/UV-VIS/ fluorometric detector) to the system controller. 9-34 CBM-20A/20Alite...
  • Page 371 In this example, set [255. 255. 255.0]. DEFAULT GATEWAY In this example, set [192.168.8.101]. When connecting to a Chromatopac or LC Workstation, select the TRS MODE communication destination. The new settings are enabled when the power is reset. CBM-20A/20Alite 9-35...
  • Page 372 Set the IP address and subnet mask in the "System Administration" tab page and set the default gateway in the "Advanced Settings" window. Enter the group and system names in order. For subsequent steps, refer to "9.4.7 Copying User Information" P. 9-41. 9-36 CBM-20A/20Alite...
  • Page 373 IP address from a DHCP server. Under normal circumstances, set the [Ether speed] to When the settings are completed, press the [0] key (quit) to save the settings. The system controller reboots and the new settings are enabled. CBM-20A/20Alite 9-37...
  • Page 374 Start Internet Explorer and enter the URL for the system controller. (URL for HPLC1: 192.168.8.161). Click the system name in the "Status Summary" section. The serial number of the system controller is displayed as the system name for new installations. 9-38 CBM-20A/20Alite...
  • Page 375 (i.e., select [-]), click and log in again. If the IP address, subnet mask, or default gateway is changed, close all Internet Explorer windows according to the on-screen instructions, and reset the system controller power. CBM-20A/20Alite 9-39...
  • Page 376 Log into the "Group Settings" application as an Administrator, just as described in "9.4.5 Setting Group and System Names" P. 9-38. Open the "User Management" tab page. Enter the user ID, name, level and temporary password, then click Register all the users. 9-40 CBM-20A/20Alite...
  • Page 377 Login as an Administrator to the "Group Settings" window for HPLC1. Click in the "Group Information" tab page to copy the user information registered for this system controller to other system controllers in the same group (HPLC2 in this example). CBM-20A/20Alite 9-41...
  • Page 378 • To copy user information, the user information from the master server is used to replace the user information for all the system controllers in a group. However, if a user exists on the master server and targeted system controller the user access level, is not changed for that user. 9-42 CBM-20A/20Alite...

  • Page 379: Specifications

    9.5 Specifications Specifications 9.5.1 CBM-20A Specification Item CBM-20A CBM-20Alite 5VDC 1A 100 to 120 or 220 to 240 VAC, Power Supply Supplied from the incorporated 50 / 60 Hz, 400 VA instrument Rated Breaking Capacity Installation Category II Installation Pollution Degree 2...
  • Page 380 1. Used for start, stop, or error output. Can also be set from a program. 2. The optional CBM-20A OPT4 is required. 3. When a Chromatopac is connected to the optical link (PAC) connector, an RS-232C connector can not be used.
  • Page 381 6. The Photodiode Array Detector can be controlled as a 4-wavelength detector from the system controller via the network. 7. When CBM-20A/20Alite version 2.40 (or later) and LabSolutions version 5.54 (or later) are connected in combination, a maximum of four column ovens can be controlled. In addition, two column ovens can be controlled by operating the CBM-20A/20Alite on the Web application window using Internet Explorer.
  • Page 382 9. Technical Information Units Controlled via Sub-controller (CBM-20A only)  Number of connectable units Maximum number of Sub- Name Model connectable Option Box Option Box Valve controller units (per Interface CBM-20A) FCV-10ALvp Solenoid FCV-10AL Valve FCV-11AL(S) (See note1.) FCV-15AL Degassing...
  • Page 383 Rack Changer C, Rack Changer II SIL-20AXR Autosampler SIL-20AC Rack Changer C, Rack Changer II SIL-20ACXR SIL-10ADvp Sample Cooler CTO-30AS 2-position Valve (flow-line switching valve) CTO-30A 6-position Valve (column-switching valve) Column Oven 2-position Valve (flow-line switching valve) CTO-20A 6-position Valve (column-switching valve) CTO-20AC CBM-20A/20Alite 9-47...
  • Page 384 Option SPD-20A SPD-20AV Detector SPD-10Avp Solvent Recycle Valve SPD-10AVvp RID-20A System Controller CBM-20A Multiple Terminal box, PC-55N 1. Cannot be used as a low-pressure gradient unit. 9.5.3 Control Specifications Control Item Internet Explorer LC Workstation Number of files Depends on PC.
  • Page 385 • RANGE setting and output of recorder signal (See note 3.) Detector • POLARITY setting (See note 4.) Fluorometric detector (RF-20A/20Axs) • ANALOG 1 MODE and ALALOG 2 MODE settings and output of recorder signal (see note 5.) • 3-wavelength mode and 4-wavelength mode settings and data display CBM-20A/20Alite 9-49...
  • Page 386 ANALOG 1 MODE: 0 (Ch1 data is output to the Chromatopac from the analog output connector 1.) ANALOG 2 MODE: 2 (Ch2 data is output to the Chromatopac from the analog output connector 2.) The RANGE (recorder range 2) setting cannot be specified. (It can be specified only when the wavelength mode is [Single].) 9-50 CBM-20A/20Alite...
  • Page 387 • Error monitoring for motor rotation • System maximum pressure (See note 1.) 1. By using the system P.MAX setting, the maximum pressure at the autosampler can be monitored. For details about the system P.MAX, see "5.12.6 System Controller". CBM-20A/20Alite 9-51...

  • Page 388: Maintenance Parts

    9. Technical Information Maintenance Parts Part Part No. Remark Fuse, 250 V 4AT S072-02004-22 CBM-20A only 9-52 CBM-20A/20Alite...

  • Page 389: Introduction To Hplc System

    9.7 Introduction to HPLC System Introduction to HPLC System The Prominence LC (LC-20A) series components are for use with Shimadzu high performance liquid chromatography (HPLC) systems, which are designed to provide high accuracy and high sensitivity analyses. Example system configurations are provided below, along with descriptions of the operations of the various components.
  • Page 390 In the column, the components are separated by means of the mutual interactions of the mobile phase and the column packing (stationary phase). The detector detects the components separated in the column, and sends the signal data to a Chromatopac or PC. Mobile phase from the detector drains into the waste container. 9-54 CBM-20A/20Alite...
  • Page 391 The detector detects the components eluted from the column, and sends the signal data to a Chromatopac or PC. Mobile phase from the detector drains into the waste container. The CBM-20A system controller can control a maximum of 8 LC components (12 LC components as an option) including a maximum of 4 pump units. CBM-20A/20Alite...
  • Page 392 26 Ethyl iodide 1.510 0.57 27 n-Octanol 1.427 10.3 28 Fluorobenzene 1.46 0.55 29 Benzylether 1.538 30 Methylene chloride(**) 1.421 0.41 0.42 0.17 31 Anisole 1.514 32 i-Pentanol 1.405 0.61 14.7 33 1,2-Dichloroethane 1.442 0.78 0.44 0.16 10.4 9-56 CBM-20A/20Alite...
  • Page 393 0.80 36.7 64 Dimethylsulfoxide 1.477 2.00 0.62 miscible 65 N-methyl-2-pyrolidone 1.468 1.67 Hexamethyl phosphoric 1.457 0.65 acid triamide 67 Methanol(*) 1.326 0.54 miscible 32.7 68 Nitromethane 1.380 0.61 69 m-Cresol 1.540 11.8 10.0 70 N-methylformamide 1.447 1.65 miscible CBM-20A/20Alite 9-57...
  • Page 394 (7) Water solubility (%W) at 20 °C of solvent used in liquid-to -solid adsorption. (8) Value at 20 °C. (9) Function consisting of p' (proportional to solvent strength) plus the dielectric constant, in ion chromatography. Source: A.M.Krstulovic and P.R.Brown, Reversed-Phase High-Performance Liquid Chromatography, Wiley Interscience, 1982. 9-58 CBM-20A/20Alite...
  • Page 395 Appendix Contents LC-30A/20A-series Network Installation Guide ........Appendix-2 Automatic Acquistion of IP Addresses from a DHCP Server ... Appendix-11...
  • Page 396 Web browser. A data-processing system, such as a Chromatopac, is required to perform data analysis. A Shimadzu analysis workstation is required for data analysis if the detector is used as a 3D detector.
  • Page 397 • Manage groups of users who are authorized to use the system. Management of system users is performed within groups. A group may contain a combined total of up to 10 CBM-20A/20Alite System Controllers and SPD-M20A/M30A PDA Detectors. Fig.2 CBM-20A/20Alite...
  • Page 398 Appendix In order to perform group settings, all the CBM-20A/20Alite System Controllers and SPD- M20A/M30A PDA Detectors must be in the same subnet, and the same group name must be set in the "Group Settings" application window. Consider factors such as the number of users for each system when deciding on the group configuration.
  • Page 399 Required when routing the LAN cable along the floor or across a wall. • Use commercial products satisfying the specifications given in the "Remark" column. • One LAN cable is required for every PC, switching hub, CBM-20A/20Alite, and SPD-M20A/ M30A.
  • Page 400 System Controller or SPD-M20A/M30A Detector, and one switching hub. Fig.3 This system can be expanded by adding to PCs, CBM-20A/20Alite System Controllers, or SPD-M20A/M30A Detectors to unused ports. To connect to an office or department network, connect one of the unused ports to a switching hub or router in the office or department network.
  • Page 401 LC-30A/20A-series Network Installation Guide Small to Medium-size Network Configurations  If there are many PCs, CBM-20A/20Alite System Controllers, and SPD-M20A/M30A Detectors, a small to medium-size network must be constructed. An example is shown below. SPD-M20A Fig.5  Points about Network Configurations •...
  • Page 402 (3) The intranet security settings must satisfy the following conditions: • The URL for the CBM-20A/20Alite must be contained in the local intranet Web site. • [Run ActiveX controls and plug-ins] of [ActiveX controls and plug-ins] must be set to [Enable].
  • Page 403 Select a name for each system that allows easy recognition. Up to 26 alphanumeric characters or symbols may be used. The symbols "-" (hyphen) and "_" (underscore) may also be used. Select a system name that is not the same as that of other CBM-20A/20Alite system or the computer name. ...
  • Page 404 The access levels are set for each system in a group and determine which users can log into each system. The Administrator is always authorized for access and all other users are authorized for access according to the default settings. Deny access if necessary. "5.9 Managing Groups" P. 5-39 Appendix-10 CBM-20A/20Alite...
  • Page 405 If the IP address is allocated from a DHCP server, ensure that the term of the IP address lease is infinite. If the IP address is changed while the CBM-20A is in operation, an error will occur at the CBM-20A and it will restart. Note that if this happens during analysis data will be lost.
  • Page 406 The CBM starts up with the allocated address.  DHCP Packets This section describes the information communicated between the DHCP server and the CBM-20A in the DHCP mode. For details on DHCP, refer to RFC2131. DHCPDISCOVER Transmission  Option Option name...
  • Page 407 Automatic Acquistion of IP Addresses from a DHCP Server Option Option name Description Vendor Specific Information DHCP servers that start with "SHIMADZU" are given priority. Server Type For other servers, DHCPDISCOVER is sent four times and the DHCP offered that is acquired fastest is selected.
  • Page 408  System Name and Host Name With the CBM-20A, the system name and host name of the network are treated as the same thing. Enter the system name of the CBM-20A as the host name of the DHCPDISCOVER/ DHCPREQUEST packet. Note also that the name designated as the host name for the DHCPOFFER/DHCPACK packet is saved as the system name.
  • Page 409 The CBM-20A allows names to be solved by using NetBIOS over TCP/IP (NBT). NBT can be used when the PC and CBM-20A are in the same subnet, and the system name of the CBM- 20A is used as the NetBIOS name. For example, if the system name of the CBM-20A is the initial value (unit serial number L20234301234), entering http://L20234301234/ as the address in Internet Explorer will start the CBM-20A’s "group monitor"...
  • Page 410 Appendix This page is intentionally left blank. Appendix-16 CBM-20A/20Alite...
  • Page 411 Fast LC ............5-145 Method Parameter ........4-12 Fatal Error ............. 6-16 Mobile Phase Characteristics ....... 9-56 Fixed (Configuration) ........3-12 Mobile Phase Reserve Volume Setting ..5-5 Fraction Collector .......... 5-21 Fraction Collector Rack Adjustment ..... 5-22 CBM-20A/20Alite Index...
  • Page 412 Startup ............5-14 Status ............2-11 Status Display ..........2-19 Status Summary ........... 2-11 Symptoms, Causes and Corrective Action ..6-2 System Administration ......5-43 9-42 System Check ........2-12 System Configuration ........3-7 System Controller ......... 9-55 System Monitor ..........2-14 CBM-20A/20Alite Index...

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