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Summary of Contents for Grant CRFT
- Page 1 Controlled Rate Freeze Thaw unit CRFT Operating Manual...
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Page 2: Table Of Contents
Electrical Connection ..................... 8 5.4. PC Setup ........................9 5.5. Switching the Equipment On ..................9 5.6. Fitting CRFT interchangeable heads ................10 Grant CRFT Software ....................11 6.1. Installation and Setup ....................11 6.3. Creating a Profile ......................16 6.4. - Page 3 Figure 21: Configuring a Rate ....................20 Figure 22: Saving a Profile to the computer ................21 Figure 23: Loading a profile into the CRFT from the PC ............22 Figure 24: Creating a backup of all profiles ................23 Figure 25: Restoring all profiles from a backup ...............
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Page 4: Introduction
Introduction 1.1. Product Description The Grant CRFT is a controlled rate freezer for biological material preservation. It is designed for use with pre-sealed vessels (e.g. CBS straws or cryovials) that act as effective containment of any possible biohazard. The CRFT is not a Medical Device as defined by Regulation (EU) 2017/745, UK Medical Devices Regulations 2002, or any other international regulations on medical devices. -
Page 5: Safety Information
It is the user organisation’s responsibility to carry out appropriate decontamination if hazardous material is spilt on or inside the equipment. Do not check the head temperature by touch as it may be very cold or very hot CRFT Operating Manual Page 5 of 50... -
Page 6: Cryopen Nucleating Tool
When using the cryopen nucleating tool (if supplied) read the instruction manual and safety instructions supplied with the product before use. Ensure that suitable safety precautions are taken during use of the Cryopen and never point the device towards the face. CRFT Operating Manual Page 6 of 50... -
Page 7: Technical Specification
< ±1.0ºC (all models) Time to achieve a stable 20ºC start temperature from switch on Unloaded head Calibrated accuracy taken as an average of 4 points at defined locations on an unloaded head CRFT Operating Manual Page 7 of 50... -
Page 8: Installation
USB communications lead Quick Start Guide Unpacking instructions If the cryopen nucleating tool has been ordered in addition to the CRFT it will also be included. Read and carefully follow the unpacking instructions included with the unit to ensure the unit is ready for installation and operation. -
Page 9: Pc Setup
Switch on the equipment unit using the power switch on the rear panel of the unit (Figure 1: Rear panel of CRFT unitFigure 1). The On/Off indicator on the front panel illuminates red when the power is switched on (Figure 2). -
Page 10: Fitting Crft Interchangeable Heads
Remove protective label. Ensure surface is clean and scratch free. Align the notch on the head to the “unlock” symbol on the CRFT case. Carefully lower the head onto the unit, and twist clockwise until the notch aligns with the “Lock” symbol. -
Page 11: Grant Crft Software
6.1.1. Loading the software The installation requires Windows 10 or Windows 11. In addition to loading the Grant CRFT software you will also be installing the drivers for the USB to serial converter (FTDI). This will all be handled by the installer. -
Page 12: Figure 5: Desktop Shortcut Icon And Windows Start Menu Search
Start the software by either double-clicking on the shortcut icon on your desktop or by selecting ‘Grant CRFT Software' from the program list in the start menu on your computer. Figure 5: Desktop shortcut icon and windows start menu search... -
Page 13: Figure 8: Connect
Wait at least 15 seconds after turning the CRFT on before trying to connect. To establish the Error! connection, click the Connect button at the bottom of the software (see Figure 8 Reference source not found. ). The CRFT and software will synchronise with each other. -
Page 14: Figure 10: Profile Selected
Figure 10: Profile selected When the equipment has reached a stable temperature the CRFT Status tile will display ‘Status: Ready’. See Figure 11 below. The ‘Ready’ orange light will illuminate on the equipment front panel and the Start Cycle button will be enabled. -
Page 15: Figure 12: User Software Overview
Disconnect number of and CRF-1 connection issue user and from CRF-1 CRF-1 status status number access level Figure 12: User Software Overview 6.2. CRFT Operating Manual Page 15 of 50... -
Page 16: Creating A Profile
Profile Editor page. Figure 13: Profile Editor page The CRFT has 10 internal slots for profiles to be saved to and are originally configured with blank profiles. When connected, editing the profiles will update the CRFT of any changes instantaneously. -
Page 17: Figure 14: Change Start Temp Of Profile
A Dwell will hold the temperature of the previous segment for a chosen length of time. • A Rate will change the Set Temperature of the CRFT and will heat or cool the Plate Temperature in a controlled way where a Rate in C/min is configurable. -
Page 18: Figure 16: Adding A Segment To A Profile
A profile has a maximum of 25 segments that can be added. There are certain rules that need to be follow when adding segments. A Rate segment must always be proceeded with a 30 second Dwell. The software should add in a Dwell above any Rate segment added. CRFT Operating Manual Page 18 of 50... -
Page 19: Figure 18: A Rate Must Be Proceeded By A Dwell
Clearing a profile will remove all segments. Click the Clear Profile button. Accept the Profile Warning to confirm the deletion of the selected profile (see Figure 19Error! Reference source not found. – the profile name highlighted in blue – in this example this is ‘Profile1’). CRFT Operating Manual Page 19 of 50... -
Page 20: Figure 19: Clearing A Profile
Temp column to edit the value within range of the selected Head fitted type, when finished press Enter on the keyboard for the changes to be saved to the unit. Ramp Rate can be edited in the same way. Figure 21: Configuring a Rate CRFT Operating Manual Page 20 of 50... -
Page 21: Figure 22: Saving A Profile To The Computer
Profiles created can be saved onto the PC such that they can be restored in the future. This also allows many profiles to be created and stored without exceeding the limit of 10 profiles available in the CRFT. Saved profiles can be loaded back into the CRFT in place of any other profile. -
Page 22: Figure 23: Loading A Profile Into The Crft From The Pc
A back up of all profiles can be saved onto the PC such that they can be restored in the future if necessary. Note: To create a backup of profiles that are installed in the CRFT then the unit MUST be switched on and connected. Taking a backup when not the CRFT is not connected will only back up offline profiles. -
Page 23: Figure 24: Creating A Backup Of All Profiles
Open dialog box and click Open. The Restore Profile Selection dialog box opens. Tick the checkboxes of the individual profiles that you wish to restore. Leave all checkboxes ticked to restore all profiles. Click Accept to confirm the restore. CRFT Operating Manual Page 23 of 50... -
Page 24: Figure 25: Restoring All Profiles From A Backup
Figure 25: Restoring all profiles from a backup 6.3.16. Completing a factory restore To completely wipe the CRFT of all profiles and reset to the default blank profiles originally set, press the Factory Restore button in the Profile Management section. Figure 26: Factory restore the... -
Page 25: Running A Profile
A warning will pop-up to confirm the resetting of the CRFT. 6.4. Running a Profile Once the profiles are setup and configured they are ready to be run on the CRFT. To select a profile to run go to the Monitor page. -
Page 26: Figure 28: Logging Settings
Figure 28: Logging settings 6.4.3. Choosing the profile to run In the CRFT Details window choose a profile from those loaded on the CRFT from the Profile drop down field. The specific temperature details of the chosen profile can be viewed by clicking on the View Details box. -
Page 27: Figure 30: Crft Initialising
6.4.4. Running the profile Once the CRFT has settled on the target temperature the green ‘Unit Ready’ light will display on the front panel of the CRFT, the Start Cycle button will be enabled allowing the profile to be run. -
Page 28: Creating Reports
Note: Not waiting for the CRFT head and samples to settle before starting may cause control problems. Note: Clicking the Stop Profile button will abort the run and the CRFT will return to the start temperature. Once the run has completed the CRFT will hold the Plate Temp to the final Set Temp until the profile is stopped. -
Page 29: Figure 32: Generating Pdf Reports
GEF log file using the dialog box which appears then click Open. The relevant report should be saved in a suitable location using the dialog box which appears (see Figure 33). Note: The TSV will not appear in the viewing area. CRFT Operating Manual Page 29 of 50... -
Page 30: Designing And Configuring Reports
The header fields on the top left and top right can be set to either an image or text using the Text box or the Logo box. Figure 34 shows an example where ‘Grant.jpg’ has been added to the left-hand Logo box and ‘Program Series A1’ text added to the right-hand... -
Page 31: Figure 34: User Defined Report Header
Text box. Figure 34: User Defined Report Header These user defined conditions will appear at the top of a generated report. Figure 35: User Defined Report CRFT Operating Manual Page 31 of 50... -
Page 32: Configuring The Software
.PDF or .TSV (Tab Separated Variable) by the reporting function. If date, time or CRFT serial number is not required in the log file name, then the default situation can be overwritten. In the example below Include Date and Include Time box have been unchecked so date or time is not in the log file name box. -
Page 33: Figure 34: User Defined Conditions
Monitor page becomes visible, see Figure 38 (with example user text of ‘Test A235’ in the User Text box which then appears in the name of the file in the Log Filename box) Figure 38: User Text CRFT Operating Manual Page 33 of 50... -
Page 34: Users
‘full stop’ for decimal point and yyyy-mm-dd for the date. Three formats are available for the date format. 6.7.6. Communication configuration options The appropriate Com Port that is connected to the CRFT can be set using this option. 6.8. Users The software is programmed with one administrator level user: User ID: Admin; Password: Admin1. -
Page 35: Figure 36: User Details
Administrator – has access to all areas of the software with the ability to make changes. Advanced – has access to Monitor, Reports, Profile Editor and Report Designer only. Standard – has access to Monitor and Reports only. CRFT Operating Manual Page 35 of 50... - Page 36 Click the user that needs to be deleted in the Quick Nav – Users window. Select Delete from the Select Mode window. Click Update to remove the user. Note: Deleting a user cannot be undone and the user that is currently logged on cannot be deleted. CRFT Operating Manual Page 36 of 50...
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Page 37: Operating Procedure For The Equipment
Operating Procedure for the equipment 7.1. Pre-operation Checks Before operating, ensure that: The CRFT has been installed, selected head has been fitted and switched on (Section 5) The software suite has been opened and communications established (Sections 6) It is recommended that an initial test run is completed using the maximum volume of liquid allowed at the fastest cooling rate to ensure that everything has been set up correctly. -
Page 38: Figure 40: 0.5Ml Cbs Straws Positioned On H00 Head
Cryovials should always be loaded starting at the centre of the head. Thoroughly dry the outside of the cryovials after preparation to prevent them from freezing to the head. Position the cryovials (Figure 42). Place the lid over the head checking that it is located correctly. CRFT Operating Manual Page 38 of 50... -
Page 39: Figure 42: Cryovials Positioned On H02 Head
Cryovials should always be loaded starting at the centre of the head. Thoroughly dry the outside of the cryovials after preparation to prevent them from freezing to the head. Position the cryovials. Place the lid over the head checking that it is located correctly. CRFT Operating Manual Page 39 of 50... -
Page 40: Starting The Cycle
- Log filename Figure 44: Monitor Panel. Ready to start Once started, the CRFT Status will change to ‘Run’ and the head temperature data is plotted on the graph. During the cycle, the temperature of the head is logged automatically in an encrypted format. -
Page 41: Figure 42: Monitor Panel. Time To Nucleation
When the samples reaches the nucleation point, the time displayed changes to Nucleate. The alarm may sound (if also configured) and the Nucleate light on the equipment illuminates. Nucleation should be done at this time. Figure 46: Monitor Panel. Nucleate CRFT Operating Manual Page 41 of 50... - Page 42 Remove the lid of the equipment completely. Turn off the buzzer by pressing either the Mute button on the CRFT keypad or on the software screen. Start the nucleating process using the chosen nucleating tool, as per their operating instructions.
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Page 43: End Of Profile
Figure 47: Producing a report when profile stopped 7.8. Starting a New Cycle To start a new cycle click Select Profile, the CRFT Status will change to Initialising again and the CRFT will start to reach temperature the instructions can then be begun again. -
Page 44: Maintenance And Service
USB comm. lead 8.3. Service & Calibration If any unit servicing is required, switch off the unit and contact Grant Instruments. Grant recommends the equipment is calibrated annually. Servicing and calibration should only be completed by Grant Instruments Service Department or via a Grant Instruments worldwide distributor. -
Page 45: Warranty
Agency; this reference number is WEE/GA0048TZ. For other countries please contact your equipment supplier. For General WEEE information please visit: www.b2bcompliance.org.uk Please note that because it contains a pressure vessel the CRFT series freezer is treated as “Special Waste”. 10.2. RoHS Directive... -
Page 46: Appendix 1 Troubleshooting
Open Device Manager in Windows and check the Ports (COM & LPT) section. Locate the Grant Instruments CRFT USB driver and ensure the driver is installed correctly by right clicking on it and selecting Properties. If the Device Status does not show ‘This device is working properly’... -
Page 47: A1.4 Straws Stuck To Head
The samples may be damp and as they fit tightly into the head they may have frozen onto the head. Ensure the samples are dry before freezing (Section 7.3). Samples can be removable with careful use of tweezers. CRFT Operating Manual Page 47 of 50 35472-V1 DMN W49... -
Page 48: Unpacking Instructions
Appendix 2 Unpacking instructions THE CRFT IS A DELICATE SCIENTIFIC INSTRUMENT AND SHOULD BE HANDLED WITH CARE AT ALL TIMES. THESE INSTRUCTIONS MUST BE FOLLOWED TO UNPACK THE UNIT. WARNING:DO NOT USE THE STRAP AS HANDLE. GRASP THE UNIT VIA THE SIDE AND BASE WHILE UNPACKING 1. -
Page 49: Appendix 3 Packing Instructions
Appendix 3 Packing instructions THE CRFT IS A DELICATE SCIENTIFIC INSTRUMENT. FAILURE TO CORRECTLY PACK THE UNIT MAY RESULT IN DAMAGE TO THE UNIT AND RENDER THE WARRANTY VOID. WARNING: ENSURE INTERCHANGEABLE HEAD IS REMOVED BEFORE PACKING THE UNIT. DO NOT USE THE STRAP AS HANDLE. GRASP... - Page 50 Grant Instruments (Cambridge) Ltd Representative in the European Union Evolution House Grant Instruments Europe B.V Unit 2 Durham Way Strawinskylaan 411 Royston Gateway WTC, Tower A, 4th Floor Royston 1077 XX AMSTERDAM SG8 5GX THE NETHERLANDS Tel: +44 (0) 1763 260811 salesdesk@grantinstruments.com...
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