Refractometer Instruction Manual
PRECAUTIONS
Never submerge the unit, and do not let liquid seep into the unit's body. Clean the refrac-
tometer after each use with a soft cotton cloth. Do not scratch surface of the prism. Store
in a dry, clean, and non-corrosive environment. Avoid strong impacts.
OPERATING PROCEDURE
1. With the Daylight Plate open, clean the Prism with a soft cloth to avoid scratching the
surface.
2. Aim the refractometer toward a light source and rotate the Eyepiece to obtain the
clearest focus.
3. Zero Point Calibration:
A. Open the Daylight Plate.
B. Apply a few drops of the of distilled water onto the Prism platform.
C. Close the Daylight Plate.
D. Turn the Calibration Screw until the dark and light boundary line coincides with the
bottom line of the scale.
4. Carefully dry the prism platform and Daylight Plate.
5. Place a few drops of the test solution on the prism and close the Daylight Plate so
the solution spreads evenly on the prism.
6. Aim the front of the refractometer towards the light source and focus the eye piece
on the boundary line.
7. The boundary line indicates the value of the test sample at 20°C.
8. After use, clean the prism with distilled water and a cloth to remove any residue.
Note: The temperature of the zero point calibration should be at the same temperature
as the test solution. For variations in temperature the zero point should be adjusted once
every 30 minutes.
SPER SCIENTIFIC
Environmental Measurement Instruments
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