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Summary of Contents for diagenode B01060002
- Page 1 U S E R M A N U A L Bioruptor Pico ® Soni ca tion Sy st em Cat. No. B01060001 - B01060002- B01060003 Version 1.2 09.15...
- Page 2 Diagenode, at its discretion, will repair or replace the product in accordance with the warranty terms and conditions stated herein. In case of repair or replacement of a product under warranty, Diagenode will cover the expenses to return the repaired or replacement product.
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Page 3: Table Of Contents
DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 3 Contents Critical steps for maintenance and efficient shearing ........4 Introduction . -
Page 4: Critical Steps For Maintenance And Efficient Shearing
PAGE 4 DIAGENODE BIORUPTOR ® PICO USER MANUAL Critical steps for maintenance and efficient shearing General warnings • DO NOT turn on the instrument without water. • DO NOT tilt the Bioruptor Pico. To change the water, use either the plastic pump or a beaker (be careful not to ®... -
Page 5: Introduction
Exposure to 20-60 kHz sound waves has not been shown to be harmful to human health. However, we would recommend avoiding unnecessary exposure. Diagenode recommends that pregnant women should not be exposed to 20-60 kHz wave lengths for a long period of time. -
Page 6: Bioruptor ® Pico Technical Specifications
PAGE 6 DIAGENODE BIORUPTOR ® PICO USER MANUAL Bioruptor Pico technical specifications ® Bioruptor Pico ® Power supply 100-230V, 50/60Hz, 2.1A (EU) / 4.2A (US) Ultrasonic wave frequency 20-60 kHz Ultrasonic wave output power 25-210 W Sonication unit dimensions 350 (W) x 245 (D) x 280 (H) mm... -
Page 7: Getting To Know Your Bioruptor
PICO USER MANUAL PAGE 7 Getting to know your Bioruptor Pico system ® Bioruptor Pico components overview ® Sonication bath Motorized lid Sonication unit Power cable (EU) Power cable (US) Tube holder Water Cooler To learn more, please visit www.diagenode.com www.diagenode.com |... -
Page 8: Single Cycle Valve For Bioruptor
The level of the water has been optimized and should always reach the red line (sticker on the wall of the tank). Distilled water should be used to fill the tank. Replacement stickers can be obtained from Diagenode. Water temperature The water in the sonication bath must be kept at 4°C. -
Page 9: Motorized Lid
DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 9 Automatic temperature control The Water Cooler (New Cat. No. B0201002, 230V; B02010003, 115V; B02010004, 100V; Old Cat. No. BioAcc-Cool) has to be used in combination with the Single Cycle Valve for Bioruptor Pico (Cat. -
Page 10: Equipment Installation
PAGE 10 DIAGENODE BIORUPTOR ® PICO USER MANUAL Equipment installation The following pages contain information on installing your particular Bioruptor Pico model. This equipment must only ® be installed by personnel after reading this section. Consider all hazards even though no particular hazards have been identified during installation. -
Page 11: Installing The Bioruptor
DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 11 Installing the Bioruptor Pico system with Water Cooler ® Before starting the installation, turn the main switches off and make sure that the unit is not plugged into an electrical outlet. 1. Open the boxes and unpack all components... - Page 12 PAGE 12 DIAGENODE BIORUPTOR ® PICO USER MANUAL 5. Insert the short red and blue tubing into the outlets of the Water cooler and the lower red and blue nozzle connectors ) of the single cylce vavle box as shown in the image. Respect the red and blue color codes.
- Page 13 DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 13 Press main switch on the front side of the Water cooler. Main Switch Fill the tank of the Water cooler with 3.5 liter and the sonication bath of the Bioruptor up to the red line of ®...
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Page 14: Controlling The Sonication
PAGE 14 DIAGENODE BIORUPTOR ® PICO USER MANUAL Controlling the sonication Digital Timer: Allows the user to easily program the sonication of samples (ON/OFF pulse time & total time). See use of digital timer in next section below. Start Button:... - Page 15 DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 15 Digital timer CYCLE NUMBER, TIME ON and TIME OFF are the Once the run started the RUNNING screen appears. As parameters controlling sonication. For changing these shown in Figure 4 the RUNNING screen indicates the parameters press the OK button.
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Page 16: Tube Holders And Tubes
PAGE 16 DIAGENODE BIORUPTOR ® PICO USER MANUAL Tube holders & tubes For chromatin and DNA shearing we highly recommend to use the below mentioned tube holders and the corresponding tubes. To guarantee homogeneity of chromatin or DNA shearing, the tube holders should always be completely filled with tubes. -
Page 17: Standard Protocol For Dna Shearing
DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 17 Standard protocols for DNA shearing Programmable DNA size distributions, excellent reproducibility, and high dsDNA 1230 bp yields with Bioruptor Pico ® 319 bp 1036 bp 234 bp Figure shows different DNA size distributions... - Page 18 PAGE 18 DIAGENODE BIORUPTOR ® PICO USER MANUAL Cycle condition Target size Cycle number (On/Off cycle time) 150 bp 30’’/30’’ 200 bp 30’’/30’’ 300 bp* 30’’/90’’ 400 bp* 15’’/90’’ 7 - 8 1000 bp* 5’’/90’’ 7 - 8 * For longer fragments (300 up to 1000 bp), a short centrifugation step after half of the cycle numbers can significantly improve the results.
- Page 19 DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 19 A. Target Size: 150 bp Excellent reproducibility and programmable size distribution with Bioruptor Pico ® Panels A to D show size distributions of sheared genomic from separate experiments aiming at producing high-quality...
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Page 20: Important Comments About Dna Shearing
PICO USER MANUAL Important comments about DNA shearing The Diagenode ACT (Adaptative Cavitation Transfer technology) process is highly reproducible. However, attention must be paid to the following treatment attributes to ensure best results: • Tubes: At present, the recommended tube vessels are the 0.65 ml Bioruptor Microtubes (New Cat. - Page 21 DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 21 2. Water bath maintenance: The water bath metal surface is fragile and requires a careful maintenance. Use only soft sponge to remove traces. Never use scratch scrub sponge since this would alter the ultrasonic wave emitter surface.
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Page 22: Small Volume Dna Shearing For Bioruptor
PAGE 22 DIAGENODE BIORUPTOR ® PICO USER MANUAL Small volume DNA shearing for Bioruptor Pico ® NOTICE: For DNA shearing we highly recommend to use the 0.1 ml tube holder & tube adaptor (Cat. No. B01200041) and the corresponding 0.1 ml Bioruptor Microtubes (Cat. - Page 23 DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 23 Sonication table for small volume DNA Shearing Target size Cycle conditions (On/Off time) Cycle number 200 bp 30”/30” 20 cycles 250 bp 30”/30” 15 cycles* 300 bp 30”/30” 13 cycles** 550 - 600 bp 15”/90”...
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Page 24: Important Comments About Dna Shearing
• Sample concentration: Diagenode recommends using a DNA concentration of 10 ng/ µl. Using larger or smaller concentrations may result in variable peak distribution. - Page 25 DIAGENODE BIORUPTOR ® PICO USER MANUAL PAGE 25 2. Water bath maintenance: The water bath metal surface is fragile and requires a careful maintenance. Use only soft sponge to remove traces. Never use scratch scrub sponge since this would alter the ultrasonic wave emitter surface.
- Page 26 HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleus isolation is performed using buffers and reagents of Diagenode’s Chromatin Shearing Optimization kit - Low SDS (New Cat. No. C01020010; Old Cat. No. AA-001-0100) 1x10e6 cells are then resuspended in 100 µl Shearing Buffer prior to chromatin shearing.
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Page 27: Consistent And Highly Reproducible Chromatin Shearing With The Bioruptor
500 μl - 2 ml Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin Shearing Optimization kit – Low SDS protocol (Cat. No. AA-001-0100). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 µl. -
Page 28: Pico
Diagenode sa. orders@diagenode.com info.na@diagenode.com www.diagenode.com info@diagenode.com © 2015 Diagenode, Inc. All rights reserved. The content of this document cannot be reproduced without prior permission of the authors. Bioruptor & IP-Star are registered trademarks of Diagenode MA-BR-PICO-1.2_09_15 Innovating Epigenetic Solutions...
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