diagenode Megaruptor 3 Instructions Manual

Diagenode shearing machine

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Megaruptor
3
®
Sleek, Innovative, Unmatched
Diagenode Shearing Machine

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Summary of Contents for diagenode Megaruptor 3

  • Page 1 Megaruptor ® Sleek, Innovative, Unmatched Diagenode Shearing Machine...
  • Page 2 Dear customer, Thank you for choosing our device and for your confidence in Diagenode. We have designed this device using our higest level of knowledge in shearing so that it exceeds your expectations. This device will serve your research needs for many years and will provide the best experience with long DNA molecules...
  • Page 3: Intended Use

    INTENDED USE The Megaruptor 3 is intended for laboratory use. It is not suitable for clinical use. Use of the Megaruptor 3 in any ® manner other than as directed herein could cause harm to persons and may void the warranty. Diagenode will not be responsible for injury or damage resulting from improper use of the Megaruptor 3.
  • Page 4 TECHNICAL SPECIFICATIONS External Power Supply Unit Voltage Range 100V AC – 240V AC 50Hz/60Hz Electrical Consumption Maximum 90W Max Main Unit Dimensions 470 (W) x 140 (D) x 25 (H) mm Total Weight 6 kg with Cassette included (0,8kg) Fragment Length Range Achievable 5 kb - ›100 kb Sample Volume Range 65-500 µl...
  • Page 5 Base unit Touchscreen Removable Cassette Sample Insert Cassette Plug Power Button Led Light Bar...
  • Page 6 INTERFACE Settings: Visualize and change the parameters of the Megaruptor 3 Initialization: Set the system to its “0” position Sound & Screen: Visualize and change the parameters of the sound and the screen Protocols: Run a shearing experiment (follow instructions)
  • Page 7: Led Light Bar

    LED LIGHT BAR The LED light bar allows the tracking of the processing of your samples. blue light means the system is waiting for the operator and is flashing if the system needs to be initialized. green light indicates the progression of the run. orange light means the protocol is on pause.
  • Page 8 Start and initialize the device Start the shearing experiment The initialization step must be performed if the icon is flashing...
  • Page 9 65 µl 200-500 µl Fragment Length Achievable 5-100 kb 12-75 kb 5-100 kb Or find your pre-recorded shearing protocol If the conditions are different from the above table, further on User protocols optimization steps are needed. Please contact Diagenode https://www.diagenode.com/en/pages/support...
  • Page 10 Sample preparation (between 65-500 µl) Spin your sample(s) Hydro Tube DNA solution in the 0-150 ng/µl range...
  • Page 11 Insert the sample(s) in the cassette (1 to 8 samples) Plug Hydro Tube Hydropore-Syringe Always set consumables (with sample or empty) at Watch the video on our each extremity of the cassette Diagenode Youtube channel How to set up your sample(s)
  • Page 12 Let your Megaruptor running Collect your sample(s)
  • Page 13 If you have any question, please contact the specialist at https://www.diagenode.com/en/pages/support...
  • Page 14: Equipment Care

    EQUIPMENT CARE Regular maintenance steps are typically unnecessary. MANUAL Read the full manual “ https://www.diagenode.com/en/p/megaruptor-3 ” - Tab Documents SAFETY APPROVAL Megaruptor 3 has been assessed and certified under the standards. ® ENVIRONMENTAL PROTECTION Packaging materials contain recyclable materials.
  • Page 15: Warranty

    If a manufacturing defect arises and a valid claim is received within the warranty period, Diagenode, at its discretion, will repair or replace the product in accordance with the warranty terms and conditions stated herein.
  • Page 16 © 2019 Diagenode, Inc. All rights reserved. The content of this document cannot be reproduced without prior permission of the authors.

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