Baker Ruskinn Bugbox UM-016 User Manual
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Baker Ruskinn Bugbox UM-016 User Manual

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UM-016
Bugbox User Manual
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Summary of Contents for Baker Ruskinn Bugbox UM-016

  • Page 1 Bugbox UM-016 Bugbox User Manual Affix Serial Number Sticker Here...

  • Page 2: Product Summary

    Bugbox Product Summary Baker Ruskinn’s anaerobic workstations are designed specifically to help microbiologists cope with rising workloads and provide the best primary isolation rates. Features and Benefits  Compact size - Your personal workstation  Quick and easy direct access with the gloveless, cuffed Ezee Sleeve system.

  • Page 3: Table Of Contents

    Bugbox Table of Contents Product Summary ........................... 2 Features and Benefits ..........................2 Overview ............................ 6 Safety Instructions ......................6 Regulatory compliance ....................7 Symbols ......................... 7 Installation and relocation .................... 8 Weight and dimensions ....................9 Gas and electrical supply requirement ..................10 Gas supply requirements ....................
  • Page 4 Bugbox Environmental gas composition control .................. 20 Environmental control overview ................20 Using the anoxic indicator strips ................. 20 Using the workstation ......................21 Using the interlock ...................... 21 Interlock overview ..................21 Opening the interlock outer door ..............21 Opening the interlock inner door ..............
  • Page 5 Bugbox Replacing the detox and catalyst sachets ............38 Replacing the mains plug fuse – UK users only ..........39 Replacing the mains fuses ................40 Service requirements ....................41 Spare parts and accessories ..................41 Overview ......................41 Spare parts and cleaning agents ..............42 Accessories......................

  • Page 6: Overview

    Bugbox Overview Please read this manual carefully to familiarise yourself with the operation and maintenance of your Bugbox workstation. Note: The workstation should be located in a well-ventilated area. Safety Instructions For your safety, the safety of others using the workstation and those around you; ...

  • Page 7: Regulatory Compliance

    Bugbox Regulatory compliance This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage Directive 2006/95/EC as well as Electromagnetic compatibility as set out in the EMC Directive 204/108/EC. Symbols Before using the Bugbox, please ensure that you are familiar with the symbols on the Bugbox. Figure 1 explains the symbols found on the Bugbox;...

  • Page 8: Installation And Relocation

    Bugbox Symbol Meaning Warning, this equipment contains high voltage circuitry. Bugbox contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. Date of manufacture in format YYYY MM Installation and relocation...

  • Page 9: Weight And Dimensions

    Bugbox Weight and dimensions The Bugbox workstation weighs approximately 45kg. Figure 2 lists the dimensions of the Bugbox workstation; Figure 2: Bugbox workstation dimensions External width 758 mm External height 640 mm External depth 650 mm Workstation chamber internal width 535 mm Workstation chamber internal height 425 mm...

  • Page 10: Gas And Electrical Supply Requirement

    Bugbox Gas and electrical supply requirement Gas supply requirements The Bugbox workstation requires 2 gas supplies;  Anoxic mixed gas (up to 5.5% hydrogen, up to 10% carbon dioxide, remainder nitrogen)  Oxygen free nitrogen. The maximum permissible concentration of hydrogen in the anoxic mixed gas is 5.5%. The use of any other gas(ses) will invalidate the warranty and may cause serious injury.

  • Page 11: Workstation Overview

    Bugbox Workstation overview Workstation layout The Bugbox workstation consists of 2 main areas; the workstation chamber and the interlock chamber. The workstation chamber is the main working area of the workstation. Access to the workstation chamber is provided by the glove ports and the interlock. The interlock consists of the interlock chamber, with an inner and an outer door.

  • Page 12: Rear View

    Bugbox Rear view Figure 5 shows the rear view of the Bugbox workstation; Figure 5: Bugbox workstation rear view UM-16 R5.0 Page 12 of 48 Bugbox User Manual...

  • Page 13: Left Side View - Standard Humidifier

    Bugbox Left side view – Standard humidifier Figure 6 shows the left side view of the Bugbox workstation with the standard humidifier; Figure 6: Bugbox workstation left side view 1. Pressure relief tank fill level indicator. 2. Pressure relief tank. 3.

  • Page 14: Left Side View - Ultrasonic Humidifier

    Bugbox Left side view – Ultrasonic humidifier Figure 7 shows the left side view of the Bugbox workstation with the ultrasonic humidifier; Figure 7: Bugbox workstation left side view with ultrasonic humidifier 1. Humidifier screw cap. 2. Humidifier tank. 3. Vacuum port (optional extra). 4.

  • Page 15: Right Side View

    Bugbox Right side view Figure 8 shows the right side view of the Bugbox workstation; Figure 8: Bugbox workstation right side view 1. Interlock outer door knob. 2. Interlock outer door. 3. Serial number label. 4. PAT test label. 5. Power entry panel. UM-16 R5.0 Page 15 of 48 Bugbox User Manual...

  • Page 16: Control System Layout

    Bugbox Control system layout Please familiarise yourself with the control system layout of your Bugbox workstation. Control panel Figure 9 shows the control panel of the Bugbox workstation; Figure 9: Bugbox workstation control panel 1. Power indicator. Illuminates to indicate the workstation is connected to the mains and is switched on.

  • Page 17: Temperature Controller

    Bugbox Temperature controller Figure 10 shows the temperature controller; Figure 10: Temperature controller 1. Not user accessible (For service personnel only) 2. Not user accessible (For service personnel only) 3. Number scrolling button. Use to move across digits. Use with buttons 4 &...

  • Page 18: Foot Switch Control Panel

    Bugbox Foot switch control panel Figure 12 shows the foot switch controller which is used to operate the spot light, vacuum control and interlock purge control; Figure 12: Foot switch controller 1. VAC for glove port vacuum control 2. LOCK for interlock purge control 3.

  • Page 19: Temperature And Humidity Control

    Bugbox Temperature and humidity control Temperature control The Bugbox workstation can control the workstation chamber temperature between ambient plus 5 and 45 The temperature in the main chamber can be set using the temperature controller on the control panel. Setting the temperature; Referring to Figure 10 ...

  • Page 20: Environmental Gas Composition Control

    Bugbox Environmental gas composition control The Bugbox workstation provides an anaerobic (oxygen free) environment for the incubation of samples and cultures. Environmental control overview To provide an anaerobic environment, anoxic mixed gas is injected into the workstation chamber. The hydrogen in the anaerobic mixed gas reacts with any oxygen in the workstation chamber in the presence of a catalyst to form water.

  • Page 21: Using The Workstation

    Bugbox Using the workstation Using the interlock The Bugbox workstation has a specially designed interlock for transferring Petri dishes into and out of the workstation chamber. Interlock overview The interlock consists of 2 main components;  Interlock outer door  Interlock inner door The interlock is accessed by opening either the outer or inner door.

  • Page 22: Transferring Material Into The Workstation Chamber Via The Interlock

    Bugbox Transferring material into the workstation chamber via the interlock To transfer material into the workstation chamber via the interlock;  Place the Petri dishes in a small Petri dish holder.  Open the interlock outer door (see section 6.1.2 for more details). ...

  • Page 23: Hand Access To The Main Chamber

    Bugbox Hand access to the main chamber Direct hand access to the workstation chamber is provided via the glove ports and the Ezee Sleeve system. The Ezee Sleeves provide direct bare hand (or gloved) access to the workstation chamber. The glove ports and Ezee Sleeves can be used in 3 ways; ...

  • Page 24: Ezee Sleeve Overview

    Bugbox Ezee Sleeve overview The Ezee Sleeves consists of a sleeve and a cuff. There are 3 sizes of Ezee Sleeve available; small, medium and large. See section 7.4.2 for ordering details. The sleeve attaches to the glove port via 2 O-rings. The cuff is then attached to the sleeve via an O- ring.
  • Page 25 Bugbox  Rotate the glove port bar until it is horizontal.  Whilst holding the glove port cover knob, push the glove port cover and your hand into the workstation chamber slowly. Place the glove port cover in the glove port cover holder. Figure 16: Glove port cover holder ...

  • Page 26: Exiting The Workstation Chamber Using The Ezee Sleeves

    Bugbox Figure 16: Glove port cover holder Exiting the workstation chamber using the Ezee Sleeves To exit the workstation chamber using the Ezee Sleeves; Remove the glove port cover from the glove port cover holder (Figure 16: Glove port cover holder ...

  • Page 27: Arm Gas Displacement Volume And Pressure Changes

    Bugbox  Holding the glove port cover by the glove port cover knob, slowly pull the glove port cover into the glove port with the glove port bar horizontal.  Rotate the glove port bar inside the glove port so that it is vertical. ...

  • Page 28: Internal Lights

    Bugbox Internal lights 2 internal lighting systems are provided for the workstation chamber;  Main chamber light.  Spot light. To switch the main chamber light on, press the chamber light switch (item 2 in Figure 9). To switch the main chamber light off, press the chamber light switch (item 2 in Figure 9). To switch the spotlight on, press and hold the spot pedal on the foot switch control panel (item 3 in Figure 12).
  • Page 29 Bugbox Figure 17: SPES external view 1. Thumb screw. 2. SPES external flap. Figure 18 shows the internal view of the SPES; Figure 18: SPES internal view 6.5.1.2 Using the SPES To use the SPES;  Undo the thumb screw on the external flap (item 1 in Figure 17). ...
  • Page 30 Bugbox Figure 19: SPES with external hinged flap opened  Push materials through the SPES internal flap into the workstation chamber. This should be done as quickly as possible to minimise gas loss from the workstation chamber.  When all materials have been added, lift the SPES external flap back to its closed position. ...

  • Page 31: Internal Power Socket

    Bugbox Internal power socket An internal power socket is provided within the workstation chamber. The socket is located on the left hand side, underneath the rear shelf. The maximum permissible power rating of equipment connected to the internal socket is shown in Figure 20.

  • Page 32: Gas Sample Port

    Bugbox Gas sample port The gas sample port can be used to collect a gas sample from the workstation chamber. To use the gas sample port;  Remove the outer cap.  Push a needle connected to a syringe through the internal sponge of the gas sample port. ...

  • Page 33: Jar Attachment Option

    Bugbox Jar attachment option The jar attachment option is used to condition anaerobic jars. To use the jar attachment option using the manual gas purge;  Attach the anaerobic jar to the anaerobic jar gas connector using the supplied connection hose, located on the power entry panel.

  • Page 34: Cleaning And Maintenance

    Bugbox Cleaning and maintenance Cleaning the workstation To ensure that the Bugbox workstation remains at optimum working conditions, it must be cleaned on a regular basis. A basic clean is required after each use. Deep cleaning is required at regular intervals, dependent upon the nature of the materials used in the workstation.

  • Page 35: Cleaning Procedure - Deep Clean

    Bugbox Cleaning procedure – deep clean To deep clean the workstation; Preparing the workstation  Remove all cells/ samples to an alternative storage facility.  Switch the workstation off at the mains and remove the plug from the mains.  Remove the Ezee Sleeves (see section 7.2 more details).

  • Page 36: Maintaining The Workstation - End User Maintenance

    Bugbox Maintaining the workstation – End user maintenance To ensure that your Bugbox workstation remains at optimum working conditions, it must be maintained on a regular basis. Many basic tasks can be performed by the end user. Filling the pressure relief/ humidifier tank The pressure relief/ humidifier tank requires refilling if the water level is on or below the low level indication.

  • Page 37: Replacing An Ezee Sleeve

    Bugbox Replacing an Ezee Sleeve To remove an Ezee Sleeve;  Ensure that the glove port covers are closed.  Remove the O-rings that hold the Ezee Sleeve to the glove port. Note: The O-rings will be tight. Figure 26 shows the O-ring removal; Figure 26: O-ring removal ...

  • Page 38: Replacing The Detox And Catalyst Sachets

    Bugbox Replacing the detox and catalyst sachets A detox and a catalyst sachet are supplied with the Bugbox workstation. The detox sachet adsorbs volatile organic compounds, improving the air quality within the workstation chamber. The catalyst sachet contains the catalyst required for the hydrogen to react with any oxygen in the workstation, to produce an anoxic environment.

  • Page 39: Replacing The Mains Plug Fuse - Uk Users Only

    Bugbox  Remove the new sachets from their packaging.  Whilst lifting the floor tray at the front, slide the sachets into the sachet holder.  Lower the floor tray, ensuring that the floor tray leg has located in the floor tray locator (item 3 in Figure 27).

  • Page 40: Replacing The Mains Fuses

    Bugbox Replacing the mains fuses To replace the mains fuses;  Switch off the Bugbox.  Remove the plug from the mains socket.  Remove the mains fuse drawer using a small flat bladed screwdriver. Figure 29 shows the removal of the mains fuse holder; Figure 29: Mains fuse holder removal ...

  • Page 41: Service Requirements

    Bugbox Service requirements To maintain the best performance from your Bugbox workstation, it must be serviced at regular intervals. Figure 30 lists the servicing requirements, intervals and persons capable of performing the service; Figure 30: Bugbox servicing requirements Action Frequency Clean workstation After each use End User...

  • Page 42: Spare Parts And Cleaning Agents

    Bugbox Spare parts and cleaning agents Figure 31 lists the spare parts and cleaning agents available for your Bugbox workstation. To order spare parts, please contact your local distributor for the latest pricing and availability. All items are sold individually except where stated. Figure 31: Bugbox end user spare parts and cleaning agents list Part Where used...

  • Page 43: Workstation Malfunction

    Bugbox Workstation malfunction In the event of a workstation malfunction, please check section 7.6 for a list of common problems and solutions. If you cannot find a solution to your problem, please contact your local distributor, quoting the serial number of your workstation. Common problems and solutions Workstation general problems Please consult the list shown below as a reference in the event of a malfunction of your workstation.

  • Page 44: Interlock Problems

    Bugbox Interlock problems Figure 34 gives a list of common interlock problems and solutions; Figure 34: Interlock problems and solutions Problem Cause Solution The interlock inner The interlock outer Check the interlock outer door and wait for the door will not open door is open interlock purge cycle to run The interlock is purging...

  • Page 45: Gas Consumption/ Environmental Control Problems

    Bugbox Gas consumption/ environmental control problems Bugbox is fitted with a gas demand indicator (item 7 in Figure 9). The indicator illuminates when gas is injected into the workstation chamber. When the workstation is at rest, the gas demand indicator should illuminate once every 20 minutes.

  • Page 46: Warranty Information

    Bugbox Warranty information Ruskinn Technology Limited warrants for the applicable time period that the Bugbox will substantially perform in accordance with the user documentation. The terms of this Agreement do not affect or prejudice the statutory rights of a consumer acquiring the Ruskinn Technology Limited Bugbox otherwise than in the normal course of a business.

  • Page 47: Disposal Information

    Bugbox Disposal information Bugbox contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. Alternatively please contact your local distributor for disposal instructions. Bugbox contains recyclable parts.

  • Page 48: Contact Details

    Bugbox Contact details Ruskinn Technology Limited Address: 8 York Park, Bridgend Industrial Estate Bridgend CF31 3TB United Kingdom Phone: +44 (0)1656 645988 Fax: +44 (0)1656 667966 Email: Sales: sales@ruskinn.com Technical support techsupport@ruskinn.com General enquiries ruskinnoffice@ruskinn.com Website: www.ruskinn.com YouTube channel http://www.youtube.com/ruskinntechnology Ruskinn Technology Limited is a registered company in United Kingdom, company number 05692599 Ruskinn Technology Limited is VAT registered in United Kingdom, VAT number 870194126 Ruskinn Technology Limited is a wholly owned subsidiary of the Baker Company...

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