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Related Manuals for Baker Ruskinn OxyGenie
Summary of Contents for Baker Ruskinn OxyGenie
- Page 1 User Manual UM-049 OxyGenie™ OxyGenie™ Affix Serial Number Sticker Here ...
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Page 2: Table Of Contents
SYMBOLS ............................. 7 TRANSPORT AND STORAGE ........................ 8 ENVIRONMENTAL OPERATING CONDITIONS .................. 8 OXYGENIE™ OVERVIEW .......................... 9 BACKGROUND ............................. 9 HOW TO USE OXYGENIE™ ........................ 1 0 POWER SUPPLY ............................. 1 1 GAS BOTTLE FITTING .......................... 1 2 GAS BOTTLES ............................ 1 4 GAS BOTTLE ASSEMBLY ......................... 1 5 ... - Page 3 Unit 8&9 / York Park / Bridgend, United Kingdom / CF31 3TB +44 (0) 1656 645688 / Fax: +44 (0) 1656 667966 / sales@ruskinn.com / www.bakerruskinn.com CHECKLIST ............................. 4 0 WARRANTY INFORMATION........................ 4 1 DISPOSAL INFORMATION ........................ 4 2 CONTACT DETAILS .......................... 4 3 TABLE OF FIGURES Figure 1 ‐ Power Supply ........................ 1 1 Figure 2 ‐ Power display messages ....................... 1 1 Figure 3 – Filling station to gas cylinder .................... 1 3 Figure 4 ‐ Filling sttion to bottle ...................... 1 3 Figure 5 ‐ Control knob opening ...................... 1 3 Figure 6 ‐ Gas bottle assembly ...
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Page 4: Introduction
INTRODUCTION Please read this manual carefully before using the OxyGenie™ and familiarise yourself with all aspects of using the product. The Baker Company (Baker) or Ruskinn Technology Ltd (Ruskinn) does not accept responsibility for accidents to personnel or damage to the OxyGenie™ resulting from incorrect use. Many unique features of the OxyGenie™ are covered in detail in this manual. It is recommended that the user be fully conversant with the instruction and procedures, and that the operator familiarises themselves with all aspects and functions of the system before it is commissioned to maintain optimum performance. ... -
Page 5: Safety Instructions
SAFETY INSTRUCTIONS Baker and/or Ruskinn do not take any responsibility for damages caused by using the equipment for other purposes than described in this user manual. This is a Class A product (for industrial environments). In a residential environment, it may cause radio interference. If radio interference occurs, the user may be required to take appropriate measures. This device contains high pressure gases. If there is gap between gas bottle and regulator do not use or fill this bottle. Place this bottle on ground on an isolated safe place to let the gas bottle to fully degas. This device contains electronics. This device contains heating elements. Do not change heating device type settings from heat plate controller. Do not replace any parts of the device or its components. This device is intended to use in research only. Device should be only used by technically qualified person. Device must be used according to manual instructions, if usage deviates from the instructions the performance can be harmed. If any damages appear, the device should not be used. If unusual performance occurs, such as deformation, discoloration, abnormal sound or smell, or abnormal heating stop using the device and contact the provider. If any liquid leaks from interior battery do not touch the fluid. Always transport the device with care and do not drop or apply any shock or vibrations to the device. Always place device on robust and stable surface. Some parts of the device can reach high temperatures, thus do not touch these parts. ... - Page 6 CAUTION: Asphyxiation Risk The OxyGenie™ uses Nitrogen (N ) and Carbon Dioxide (CO ) as part of normal use with the volume released externally is inconsequential. In the event of a leak or malfunction this gas release may become excessive. DO NOT OPERATE this unit in a SMALL ENCLOSURE such as a small room or walk‐in closet. An accidental release of Nitrogen or Carbon Dioxide could create an asphyxiating atmosphere in a small ...
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Page 7: Regulatory Compliance
REGULATORY COMPLIANCE European Region This product complies with the essential EEA requirements for Electrical Safety and Electromagnetic compatibility as set out in the EMC Directive 2014/30/EU, the Low Voltage Directive 2014/35/EU and hazardous substances (RoHS) Directive 2011/65/EU has been tested and found to comply in full. For details on standards tested refer to the DoC Certificate. WEEE: ... -
Page 8: Symbols
the Hazard. Warning, this equipment contains high voltage circuitry. Contains material or substances that may be hazardous to human health. Please refer to your local biohazardous material handling procedure for further advice on the handling and disposal of these items. OxyGenie™ contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. USB socket Date of manufacture in format 07/2019 MM/YYYY Table 1: List of Symbols 7 ... -
Page 9: Transport And Storage
TRANSPORT AND STORAGE When not in use, the OxyGenie™ must only be stored within a temperature of between 0°C and 30°C Storage outside of this range may cause damage. ENVIRONMENTAL OPERATING CONDITIONS The OxyGenie™ should only be operated under the following environmental conditions: Temperature – Between 15⁰C and 30⁰C Humidity – Between ambient and 90% RH, Non‐Condensing The OxyGenie™ must be located in a well‐ventilated area. 8 ... -
Page 10: Oxygenie™ Overview
OXYGENIE™ OVERVIEW Background OxyGenie™ is a mini‐incubator for cell culture studies. Gas bottles containing different gas mixtures will allow you to control gas environment inside the cell culture structures. With this device, oxygen levels can be controlled while pH and temperature parameters are maintained stable. 1. 8. 2. 9. 4. 10. 11. 5. 6. 12. OxyGenie™: Heat plate Device power switch/battery and power 6‐well flow divider display Open/close bottle valve (separate for each Cover gas bottle) Lid Lock Gas channel selection valve 10. Lid ... -
Page 11: How To Use Oxygenie
HOW TO USE OXYGENIE™ Check/switch gas Prepare device bottles •Select gas bottle from bottle selection valve and open the •Check gas volume bottle valve •Let system equilibrate Prepare cells Check the device Add structures to 6‐well flow divider •Assemble 1‐well •Check the valves structure •Check the heat plate •Check the covers components and add •Check that the device is •Connect flow divider cover charging gas tubes to covers 10 ... -
Page 12: Power Supply
POWER SUPPLY Use only the power supply provided with device. Note that device charges battery only when turned on. Battery life of this device is about 2 hours. Press grey switch button for 3 seconds to turn on the device. (In picture) Press heat plate controller switch button to turn on heat plate. (See heat plate controller) Beep test is disabled in this device. To shut down the whole device, switch off heat plate from the controller switch button and press grey switch button for 3 seconds. Figure 1: Power Supply Power display messages Device Low battery, Power off performs quick put device on ... -
Page 13: Gas Bottle Fitting
GAS BOTTLE FITTING Do not attach any components or fill gas bottles until you have read and understood all of these instructions. Always wear eye protection and heavy gloves when handling high pressure gases. Always point every air systems in safe direction away from yourself and others while degassing and filling the gas bottles. Do not fill bottles with gas mixtures containing oxygen more than 21% at any circumstances. Do not use compressed air or other particle and/or moisture contaminated gasses at any circumstances. Always check that all your components are unharmed without any visible fractures and flaws (see page 10) Do not use excessive force when handling any of components or filling stations. Do not fill the bottles more than 1500 PSI at any circumstances. Note that gas bottles can not be filled with more than pressure contained in your filling gas cylinder. Always mark the gas composition in your gas bottles. Fill station must be purchased separately. Without filling station bottles are not possible to be filled. Filling gas cylinders must be purchased from local gas supplier. Ask the right fitting for the cylinder threads from your local gas supplier. Threads in the filling station is ¼” NPT male threads. Make sure that the fitting is with correct threads for the cylinder. Cylinder threads are different in different countries. Ask safety instructions from local gas supplier. Filling gas cylinder must be properly secured from inclining or falling before attaching filling station to it. Confirm that fitting is compatible with your fill station. ... -
Page 14: Figure 3 - Filling Station To Gas Cylinder
WARNING! Always operate on completely empty filling station. Tighten the filling station to gas cylinder. Make sure it is properly attached. Make sure that filling station CONTROL KNOB is turned all the way counter‐clockwise. Do not use force to operate the knob. Open gas cylinder slowly and let the pressure flow to the filling station. BULK gauge shows pressure left in filling gas cylinder. OUTPUT gauge show pressure that will be dispensed to your gas bottle. Figure 3 – Filling station to gas cylinder Dispensed pressure can be adjusted by the output pressure ADJUSTMENT SCREW. Pressure can be set by turning the screw with 3/16 inch Allen key. By turning clockwise the output pressure will rise and counter‐clockwise it will decrease. After you have set the desired pressure (maximum 1500psi), let air out by turning control knob and you will notice that the output pressure changes from set value. Repeat this pressure adjustment and air release cycle until the pressure reaches desired value after the air is released. This venting step is important especially when values are set to lover range. Check the unit ALWAYS before use, because the ... -
Page 15: Gas Bottles
GAS BOTTLES 4. 1. 7. 8. 5. 2. 6. WARNING! 3. If there is gap between gas bottle and regulator, do not use or fill this bottle. Place this bottle on ground on an isolated safe place to let it fully degas and contact provider. ... -
Page 16: Gas Bottle Assembly
GAS BOTTLE ASSEMBLY 2. 1. Tighten gently gas bottles to places indicated by numbers 1 and 2. Do not use excessive force to tighten the bottles. 2. Open only one bottle valve (#2 in the picture) by turning gently all the way to the left. Do not use excessive force. 1. 3. Turn the gas channel selection valve to correct position. Pointy head indicates which bottle valve is open. Note that only one bottle can be open at a time. 3. Figure 6 ‐ Gas bottle assembly CHANGING THE GAS BOTTLE 1. -
Page 17: Gas Cylinder Adapter
Gas cylinder adapter allows gas to be fed from larger gas cylinders, thus allowing prolonged experiment time without changing gas bottles. Attach the adapter same way as gas bottles are attached to the device. Attach 4 mm (5/32“) polyurethane tubing between adapter and gas cylinder regulator. Attach gas cylinder adapter to device and adjust gas cylinder regulator to 1 bar. Make sure that bottle valve is open and gas channel is selected correctly. Note! The OxyGenie™ bottle valve cannot close the gas flow. Gas flow must be closed from the gas cylinder regulator. User must purchase tubing, cylinder regulators and gas cylinders from other providers. ... -
Page 18: 1-Well Assembly And Cell Cultivation
1‐WELL ASSEMBLY AND CELL CULTIVATION Always use new and sterile 1‐well structures. 1‐well structures, lids and lid locks, covers and cover locks are compatible with autoclave, EtOH and short term UV sterilization. 1‐well structure bottom can be coated before culturing cells. Cells can be cultured in these structures by following regular protocols. How to prepare structures to OxyGenie™: Add in cell culture medium and other necessary substances. ! Optimal volume for substances is 1 ml. Place the lid over the 1‐well structure. ! Make sure it fits correctly. Place the lid lock around the 1‐well structure and the lid. ! Make sure it grips the lid tightly and correctly around the structure. 4. When all these components are placed correctly, the cover can be added to encase the whole 1‐well structure. 17 ... -
Page 19: Cover
1. Place cover lock on the groove of 1‐well structure so that little shoulder is upwards 2. Place the cover on top of the 1‐well structure. The cover lock will allow the cover squeeze tightly around silicone structure. 3. Make sure the cover is pushed to the bottom. 1‐well structures are now ready to be assembled to OxyGenie™. If covers are not adjusted all to way to the bottom, they may leak and affect to the gas concentrations inside the cell culture. Tip: Use very thin layer of silicone grease in the lip of the cover [1]. That will help the ... -
Page 20: Adding 1-Well Assembly To System
ADDING 1‐WELL ASSEMBLY TO SYSTEM Before adding 1‐well assembly to the OxyGenie™ system, make sure that you have a correct gas composition and enough gas in the bottles. Also check that bottles/gas cylinder adapter are properly assembled. Confirm that the covers are assembled correctly. 6‐well flow divider can be wiped with 70% EtOH. Ensure that the gas bottles are open and gas is flowing. Tip: Tubes coming from flow divider can be submerged in small amount of EtOH (e.g. in small centrifuge tube) and check that bubbles come out to ensure gas flow. ... - Page 21 6‐well flow divider can be separated from the heat plate by opening locks. The 6‐well flow divider can be lifted and separated from the rest of the system. Gas flow and temperature are maintained as long as gas is connected and flow divider is resting on the heat plate. Length of these tubing and wires are 1,5 m. 20 ...
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Page 22: Heat Plate By Okolab
HEAT PLATE BY OKOLAB Heat plate is controlled with separate controller unit, H401‐T‐CONTROLLER (Okolab). Setting are initially in default temperature 37 °C and when device is turned on controller starts to reach this set point. Few quick tips and pictures are collected from Okolab H401‐T‐CONTROLLER Temperature Controller User’s Manual. For more information see Okolab user manual from their webpage. port Temperature temperature sensor port data log Power cord 21 ... - Page 23 How to plug in temperature sensor: How to plug in USB drive: 22 ...
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Page 24: Homepage
User can define Status LED device name from settings Return to homepage Homepage Temperature Heatplate set point options and settings Current temperature Overview value page Switch display Temperature mode from external temperature temperature within time... -
Page 25: Status Led Colours
Status LED colours There is a colourful dot on the touch screens homepage and colourful led on top of the screen indicating the state of the heat plate. Green indicates that set‐point have been reached and system is working properly. The set point is defined with tolerance that you have set. Yellow indicates that system is working towards reaching set point. No actions is required and this appears every time you change the set point temperature. Orange indicates that the current temperature value is out of tolerance defined in the alarm settings. Controller triggers alarm and will start work toward recovery from out‐of‐range value. Check your device for misplacements. ... -
Page 26: Settings; Devices
Settings: Devices This menu allows to control heating device type, name of the device and maximum heating rate. Type: The heating device type is initially defined as Glass plate square. This is device 1. Device 2 is initially defined as None. WARNING do not change heating device type from device 1. (Glass plate square) or device 2. (None). If these settings are changed it will cause error to device function and possibly cause danger to user. Label: This menu will allow user to change the device name. ... -
Page 27: Settings; Calibrations
Ramp rate: This menu allow user to define maximum heating rate for the heat plate. Select the maximum heating rate from slide bar or + or – and press save. WARNING If heating ramp rate is defined in high values, this may lead heating device overshoots and the temperatures can reach significantly higher value than defined set value. Settings: Calibrations This menu calibrates heating device and temperature sensor. User is recommended to calibrate heating device ... - Page 28 Letter behind Offset value mark for automatic calibration (A) or manual calibration (M). Calibration provides offset value that marks the temperature difference between liquid inside 1‐well structure and the heat plate. The external temperature sensor is provided with OxyGenie™ device. This sensor can be immersed in water and oil. How to calibrate: Click Heating device icon. Always select Device 1 ( Device 2. is disabled) ...
- Page 29 Automatic calibration: With this procedure heat plate temperature is modified until the temperature of the liquid inside 1‐well structure reaches set point and remain stable within user defined tolerance range (accuracy). After completing steps 1‐7 click “Auto”. If password is set that has to be inserted before continuing to name typing. If password is not defined, procedure will continue straight to name typing. Type in name that can be later to be used to track down calibrations. ...
- Page 30 Automatic calibration is successful when the display shows offset value. After calibration detach temperature sensor from 1‐well structure and start using the device. The touch screen is possible to be used while calibration is ongoing. Calibration procedure compilation screen will appear automatically. Manual calibration: This procedure modifies manually the heat plate temperature until temperature sensor reaches set point value. ...
- Page 31 After calibration detach sensor from 1‐well structure and start using the device. If room temperature changes more than 1°C during calibration the calibration will not be successful. Accuracy in the liquid will not be achieved by ± 0.3°C. Direct air drafts to device will prevent successful calibration. Previous calibrations Previous calibrations are saved and by clicking “Log” list of previous calibrations is shown. Click certain calibration row to view. That value can be used to the device by clicking yes. By clicking “To USB” calibration data can be downloaded to USB drive. By clicking “Erase” calibration data is deleted. For this function it is possible to define password. ...
- Page 32 Temperature sensor calibration Click T sensor icon Temperature sensor is calibrated initially at 35.0 °C, 37.0 °C and 40.0 °C. Factory settings can be seen by clicking “Factory”. Re‐calibration of temperature sensor: 1. Set water bath to the set point temperature used during experiments (37 °C most of the time). 2. Immerse temperature sensor to water bath with a certified thermometer. 3. Type in the temperature value from thermometer by pressing pencil ...
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Page 33: Settings; Data Logging
Settings: Data logging Calibration data and temperature data can be stored to device internal memory or directly to USB drive. To log data to internal memory: 1. Click Logging icon. From Log subpage click “Internal memory”. 2. Click pencil icon to set logging interval in range of 1‐60 seconds. 3. Choose how the data is split into files. If “Day” mode is chosen, each day temperature data is stored in separate file. ... - Page 34 6. By pressing screwdriver icon, the logging starting date, available memory and latest download can be seen. Available memory is dependent on logging time. You can save internal memory by decreasing logging time interval. To save data to USB drive: From subpage clicked from screwdriver icon saved data is possible to download to USB ...
- Page 35 Log data to USB drive: 1. USB pen drive must be connected to the back of the device. (See page 20) USB drive icon activates in the homepage when connected. 2. Click Logging icon. From Log subpage click “USB pendrive”. 3. The device reminds that USB drive must be connected at all time during logging. 4. Click pencil icon to set logging interval in ...
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Page 36: Settings; Alarms
Settings: Alarms Alarm can be set to inform if temperature deviates from set point for defined period of time. Alarm can be beside visual also audible. Click alarms icon to set alarm: 1. Define the time that heat plate temperature may remain outside allowed tolerance before triggering alarm. 2. Define the tolerance value which temperature cannot go over or below. 3. Define transient time meaning the time that system can take to reach a new set point without triggering the alarm. ... -
Page 37: Settings; Display
Settings: Display Click display icon to access display menu. From option icon the time frame for minimum and maximum temperature displayed in homepage can be modified. Also from this subpage touch screen sound can be disabled and sound frequency can be modified. After adjustments press “Save”. From brightness icon display brightness can be modified. After adjustments press “Save”. ... - Page 38 From Date & time icon date and time values can be set. Time can be displayed either 24 or 12 h format. After setting time and date press “Save”. From visual effects icon top led and glance mode settings are controlled. Top LED settings control when the top led is illuminated. “Never” settings will disable top led, “On Alarm” led will illuminate only when device is on alarm status and “Always” setting ...
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Page 39: Settings; Password
Settings: Password Password is possible to set for following procedures: ‐ Heating devices calibration procedures ‐ Temperature sensor calibration ‐ Data download ‐ Data erase To set password click password icon. Flag password enable to enable password. Default password is okolab. To set password click change password and follow instructions. 38 ... -
Page 40: Settings; Summary
Settings: Summary The summary page reports technical information and summary about the Okolabs heating device settings. 39 ... -
Page 41: Checklist
CHECKLIST 4. 5. 1. 3. 1. Check the gas bottles. Make sure there are enough gas and correct gas composition. 2. Check that correct gas bottle valve is open and the other is closed. 3. Check that the gas bottle selection valve is pointing to the correct position. 4. Check that 6‐well flow divider is resting right above the heat plate 5. Check that covers are adjusted tightly around the 1‐well structures. 6. Check settings for heat plate. System is now ready to use. When possible keep device on charging. If the battery is fully charged the whole system is portable and will maintain all functions with a battery life of 2 hours. 40 ... -
Page 42: Warranty Information
WARRANTY INFORMATION Ruskinn Technology Limited warrants for the applicable time period that the OxyGenie™ will substantially perform in accordance with the user documentation. The terms of this Agreement do not affect or prejudice the statutory rights of a consumer acquiring the Ruskinn Technology Limited OxyGenie™ otherwise than in the normal course of a business. THIS WARRANTY DOES NOT APPLY IN THE FOLLOWING CIRCUMSTANCES: (A) IF THE Ruskinn Technology Limited OxyGenie™ HAS BEEN REPAIRED BY PERSONS NOT AUTHORIZED BY Ruskinn Technology Limited; OR (B) THE Ruskinn Technology Limited OxyGenie™ and associated accessories/peripherals HAVE BEEN ALTERED, MODIFIED, OR MISUSED; OR (C) THE Ruskinn Technology Limited OxyGenie™ IS USED WITH NON‐ Ruskinn Technology Limited ... -
Page 43: Disposal Information
DISPOSAL INFORMATION OxyGenie™ contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. Alternatively, please contact your local distributor for disposal instructions. OxyGenie™ contains recyclable parts. Please contact your local distributor for more advice 42 ... -
Page 44: Contact Details
CONTACT DETAILS Ruskinn Technology Limited Address: 8 & 9 York Park, Bridgend Industrial Estate Bridgend CF31 3TB United Kingdom Phone: +44 (0)1656 645988 Fax: +44 (0)1656 667966 Email: Sales: sales@bakerruskinn.com Technical support: techsupport@bakerruskinn.com General enquiries: ruskinnoffice@bakerruskinn.com Website: www.bakerruskinn.com YouTube channel http://www.youtube.com/ruskinntechnology Ruskinn Technology Limited is a registered company in United Kingdom, company number 05692599 Ruskinn Technology Limited is VAT registered in United Kingdom, VAT number 870194126 Ruskinn Technology Limited is a wholly owned subsidiary of The Baker Company ...
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