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FB128 Neural Simulator Overview The FB128 Neural Simulator is a tool for testing experimental paradigms during the design phase and debugging problems when they arise. The compact, battery operated device simulates neurological waveforms or sine waves that can be output directly to a ZIF-Clip headstage.
Hardware Set-up When using the FB128 to test your protocol or recording hardware, set-up the recording part of your system as you would during your experiment with the FB128 in place of the subject and electrodes. ZIF Connectors Four output connectors are positioned side-by-side at one end of the simulator.
Failure to hold the clip open until it is fully in position can cause damage to the headstage connectors. Omnetics Connectors The FB128-OMN has 16- and 32-channel Omnetics connectors for LP16CH-Z and LP32CH-Z style headstages. Connect the headstage to the appropriate connector just as you would connect it to an electrode or adapter.
FB128 Neural Simulator | 7 LP16CH-Z and FB128-OMN Simulation Modes The Mode button, is positioned on the end opposite the output connectors. To cycle through the operating modes: • Briefly press the Mode button. The active mode is indicated by a lit LED on the face of the module.
FB128 Neural Simulator | 8 Pictured waveforms were generated by the FB128 and plotted in Synapse with the following settings: Wave Filter Settings: High Pass: 300 Hz, Low Pass: 5000 Hz LFPx Filter Settings: High Pass: 0 Hz, Low Pass: 300 Hz HASH NORMAL mode but with spikes scaled down by a factor of 2.
FB128 Neural Simulator | 9 Pictured waveforms were generated by the FB128 and plotted in Synapse with the following settings: Wave Filter Settings: High Pass: 300 Hz, Low Pass: 5000 Hz LFPx Filter Settings: High Pass: 0 Hz, Low Pass: 300 Hz LFP ONLY NORMAL with spikes scaled to zero.
FB128 Neural Simulator | 10 Pictured waveforms were generated by the FB128 and plotted in Synapse with the following settings: Wave Filter Settings: High Pass: 300 Hz, Low Pass: 5000 Hz LFPx Filter Settings: High Pass: 0 Hz, Low Pass: 300 Hz...
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FB128 Neural Simulator | 11 Shown: Tetrode + Excitatory Mode Pictured waveforms were generated by the FB128 and plotted in Synapse with the following settings: SUwv Filter Settings: High Pass: 300 Hz, Low Pass: 5000 Hz RAWx Filter Settings: Unfiltered Important To better view Tetrode mode (as pictured above) the channels must be re-mapped.
Neurological LFP waveforms with spikes on all channels firing synchronously at 100 Hz fixed rate. The spikes in this mode are always the same shape. Pictured waveforms were generated by the FB128 and plotted in Synapse with the following settings:...
FB128 Neural Simulator | 13 TONE 1000 Hz 1000 Hz sine wave at approximately ±70 uV on all channels (actual frequency is ~763 Hz). TONE REF 100 Hz sine wave at approximately ±700 uV on Reference channel only. All other channels output 0.
This is useful for mapping channels or for detecting shorts on the headstage. Press and hold the MODE button on the FB128 until the LED starts flashing, which indicates the FB128 is in channel mapping mode. Each channel plays a frequency encoded waveform that creates a unique binary pattern that can be mapped to channel number.
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Omnetics 32 Channels* * If using a ZIF headstage with a ZCA-OMN adapter, use the Mapper gizmo in Synapse and select the appropriate Ommnetics adapter from the "TDT" category of maps. Power cycle the FB128 to exit Channel Mapping Mode.
FB128 Neural Simulator | 16 Power The FB128 is powered by a 1950 mAh battery with a 10-hr life. A 6 Volt charger (tip negative) is supplied for charging. FB128 Technical Specifications Output ±10 mV Battery 1950 mAh Battery life 10 hr runtime, time to charge 2.5 hr...