cytiva Ettan IPGphor 3 Operating Instructions Manual
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cytiva Ettan IPGphor 3 Operating Instructions Manual

cytiva Ettan IPGphor 3 Operating Instructions Manual

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Ettan™ IPGphor™ 3
Operating Instructions
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Summary of Contents for cytiva Ettan IPGphor 3

  • Page 1 Ettan™ IPGphor™ 3 Operating Instructions Original instructions cytiva.com...

  • Page 2: Table Of Contents

    Use the Ettan IPGphor Cup Loading Manifold .................. 49 Start up the instrument ..........................54 Perform a run from the control panel ..................... 55 5.3.1 Ettan IPGphor 3 control panel functionality ..................56 5.3.2 Protocol parameters ........................... 59 5.3.3 Set protocol parameters ........................... 60 5.3.4...
  • Page 3 Eurasian Economic Union (Евразийский экономический союз) .......... 102 8.3.5 North America ............................... 104 8.3.6 Regulatory statements ..........................105 8.3.7 Declaration of Hazardous Substances (DoHS) ................106 Ordering information ........................... 108 Health and Safety Declaration Form ...................... 109 Index ........................... 111 Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 4: Introduction

    About this chapter This chapter contains information about these Operating Instructions, important user information, and lists of associated documentation. In this chapter Section See page Important user information About this manual Associated documentation Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 5: Important User Information

    Ettan IPGphor 3 control panel or from a PC running the Ettan IPGphor 3 control software. Ettan IPGphor 3 is intended to be used as a laboratory unit for research purposes and must only be used by properly trained personnel.

  • Page 6: About This Manual

    This manual provides information needed to install, operate and maintain the product in a safe way. Scope of this manual The document provides you with the instructions needed to use the Ettan IPGphor 3 Isoelectric Focusing System together with Ettan IPGphor 3. Notes and tips...

  • Page 7: Associated Documentation

    Introduction This section describes associated documentation and how to find related literature that can be downloaded or ordered from Cytiva. • Ettan IPGphor Cup Loading Manifold User Manual contains instructions for first- dimension isoelectric focusing of proteins on IPG strips.

  • Page 8: Safety Instructions

    All users must read and understand the entire contents of this general safety chapter, and the specific safety precau- tions information in each subsequent chapter of this manual to become aware of the hazards involved. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 9: Safety Precautions

    It is important not to proceed until all stated conditions are met and clearly understood. NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 10 Only properly trained personnel are allowed to operate and main- tain the product. WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. Personal protection WARNING Always use appropriate Personal Protective Equipment (PPE) during operation and maintenance of this product.
  • Page 11 Supply voltage. Before connecting the power cord, make sure that the supply voltage at the wall outlet corresponds to the requirements for the instrument. WARNING Protective ground. The instrument must be connected to a grounded power outlet. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 12 • damage caused by splashing liquid onto the product. WARNING The instrument must never be used if any safety functions are out of order. Contact your Cytiva service operator for more informa- tion. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 13 Isoelectric Focusing System is restarted. WARNING The vents at the front, back, and bottom of Ettan IPGphor 3 must not be obstructed. Air must be free to circulate for the cooling apparatus to function correctly. There should be a minimum of 10 cm unobstructed space behind the rear panel of the instrument.
  • Page 14 Disconnect power. Always disconnect power from the instru- ment before performing any maintenance task. WARNING Ettan IPGphor 3 covers must not be opened by the user. It contains electrical circuits which can give a lethal electric shock. Service and planned maintenance should be performed by personnel author- ized by Cytiva.

  • Page 15: Labels

    2.2 Labels Labels Introduction This section describes the system label and the safety labels on Ettan IPGphor 3. System label The system label is located on the back of the equipment. The system label identifies the equipment and shows electrical data, regulatory compliance, and warning symbols.

  • Page 16: Emergency Procedures

    2.3 Emergency procedures Emergency procedures Introduction This section describes how to perform an emergency shutdown of Ettan IPGphor 3 and the result in the event of power failure. Emergency shutdown Switch off power to the instrument by pressing the Power switch to the 0 position (1). If required, disconnect the mains power cord (2).
  • Page 17 When the system is connected, the log event will be stored in the system log. If you wish to continue a run, set up and run a new experiment with the appropriate/remaining steps to complete the run in the best way. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 18: System Description

    3 System description System description About this chapter This section provides a description of Ettan IPGphor 3 Isoelectric Focusing System and an overview of its components. In this chapter Section See page Illustrations of Ettan IPGphor 3 Ettan IPGphor 3 instrument...

  • Page 19: Illustrations Of Ettan Ipgphor 3

    Ettan IPGphor 3 control software Ettan IPGphor 3 instrument Note: Ettan IPGphor 3 instrument is used together with separate strip holders or a manifold. Two types of manifolds are available; the ceramic manifold and the light manifold. Ettan IPGphor 3 Isoelectric Focusing System also includes Immobiline DryStrip gel strips, which contain an immobilized pH gradient (IPG).
  • Page 20 3 System description 3.1 Illustrations of Ettan IPGphor 3 Ettan IPGphor 3 platform Part Function Safety lid Positive (anode) electrode area Negative (cathode) electrode area Control panel Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 21 3 System description 3.1 Illustrations of Ettan IPGphor 3 Ettan IPGphor 3 control panel Ettan IPGphor 3 is controlled by a 7-key membrane keypad. The instrument status is indicated on the LCD screen. Part Function START button STOP button LCD screen (Main screen)
  • Page 22 Ettan IPGphor 3 rear panel Electrical and communication connections are placed on the rear panel of Ettan IPGphor 3. Part Function Power switch Fuse holder USB Type-C port for connection to computer Power inlet Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 23: Ettan Ipgphor 3 Instrument

    The voltage is cutoff when the safety lid is opened. Lid adapter for strip holders When using Ettan IPGphor 3 standard strip holders a lid adapter must be used to apply the correct amount of pressure to the lids of the Ettan IPGphor 3 strip holders.

  • Page 24: Ettan Ipgphor 3 Control Software

    Isoelectric Focusing System. The software provides data presentation, data storage, and protocol handling. The PC is connected to Ettan IPGphor 3 by a USB Type-C cable. Ettan IPGphor 3 control software controls up to four Ettan IPGphor 3 systems at one time, each running a different set of run parameters.

  • Page 25: Strip Holders

    (i.e. 30 μL maximum for both wells). Introduce the sample through the wells if it is not included in the rehydration solution. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 26 IEF by pressing the strip against the electrode while allowing electrolysis gases to escape. Rehydration and separation progress can be monitored visually through the trans- parent cover. Part Function Pressure block Cover Base Sample application walls Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 27: Installation

    4 Installation Installation Introduction This chapter provides information about safe installation of Ettan IPGphor 3 Isoelectric Focusing System. In this chapter Section See page Site requirements Unpacking and transport Setup Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 28: Site Requirements

    If condensation occurs, the unit may require conditioned air to reduce temperature and relative humidity. Power requirements Parameter Specification Mains supply voltage 100-240 V AC ±10% Frequency 50/60 Hz Power consumption 230 VA Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 29 Transient overvoltages Overvoltage category II Computer requirements Ettan IPGphor 3 control software is compatible with computers that have Microsoft Windows 10 operating system and a USB port. The computer must have Microsoft Excel (Microsoft Excel for Microsoft 365 or later) installed to be able to export and save run data, graphs and log files.

  • Page 30: Unpacking And Transport

    Please keep all packing material and use if damaged parts need to be returned for repair or replacement. • Check the equipment for damage before starting assembly and installation. • Document any damage and contact your local Cytiva. • Remove straps and packing material and stand equipment upright before starting installation.

  • Page 31: Setup

    Step Action Select a place for Ettan IPGphor 3 where it is possible to access the mains power switch on the rear panel of the instrument. Position the instrument on a flat surface with the spirit level in the center.
  • Page 32 Turn on the instrument and run a diagnostic program according to the description in Section 5.2 Start up the instrument, on page Software installation Follow the steps below to install the Ettan IPGphor 3 control software. Step Action Unistall the previous version of Ettan IPGphor 3 control software, if present.
  • Page 33 Ettan IPGphor 3 is connected to a computer with a USB Type-C cable. The USB Type-C port is placed at the rear of Ettan IPGphor 3. Use the USB Type-C to USB cable that comes with Ettan IPGphor 3 to connect the USB Type-C to the appropriate USB port of the computer.
  • Page 34 Section 5.4 Run a pre- programmed protocol from the PC, on page Assembly The following parts must be added to Ettan IPGphor 3 instrument before it can be used: • Strip holder or manifold Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 35 4 Installation 4.3 Setup • Strips • Buffer and sample liquids Spare parts and accessories For correct up to date information on spare parts and accessories visit: cytiva.com Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 36: Operation

    This chapter provides instructions for the use of Ettan IPGphor 3 Isoelectric Focusing System. Before starting the instrument, rehydrate the samples according to the method selected, either fixed-length Ettan IPGphor 3 strip holders or use the Ettan IPGphor Cup Loading Manifold. In this chapter...

  • Page 37: Preparations Before Start

    30ºC to 40ºC as isocyanate, a urea degradation product, will carbamylate the proteins in the sample, thus changing their isoelectric points. NOTICE All chemicals should be of the highest purity. Double distilled water should be used. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 38: Use The Strip Holders

    Use the strip holders Introduction Ettan IPGphor 3 Isoelectric Focusing System fixed-length strip holders allow IPG strips to be rehydrated and samples loaded in one step before proceeding automatically to perform the separation. The IPG strips are 3 mm wide and 0.5 mm thick after rehydra- tion.
  • Page 39 The correct volume to each strip length is found in the instructions attached to each Immobiline DryStrip package. Step Action Pipette the solution slowly at a central point in the strip holder channel away from the sample application wells. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 40 5 Operation 5.1 Preparations before start 5.1.1 Use the strip holders Step Action Remove any larger air bubbles. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 41 IPG gel is in contact with the strip holder electrodes at each end. The gel can be visually identified once the rehydration solution begins to dye the gel. Be careful not to trap bubbles under the strip. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 42 Rehydration proceeding Rehydration can proceed on the bench top or on the Ettan IPGphor 3 platform. Ensure that the holder is on a level surface. A minimum of 10 h is required for rehydration, overnight is recommended.
  • Page 43 Electrode pads must be damp, not saturated or dripping. Lift one end of the rehydrated IPG strip using forceps or tweezers. Position an electrode pad over the electrode, then lower the strip back in place. Repeat at the other end. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 44 Introduce the sample below the cover fluid. Up to 7.5 μL of sample solution can be added to each side (i.e. 15 μL per well or maximum 30 μL if both sides of both wells are used). Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 45 5.1 Preparations before start 5.1.1 Use the strip holders Position the strip holders Ettan IPGphor 3 platform has two electrode areas: • The larger area (3) is the positive electrode (anode) • The smaller area (4) is the negative electrode (cathode).
  • Page 46 When using the Ettan IPGphor standard strip holders, lid adapters must be used to apply the correct amount of pressure to the lids of the IPGphor strip holders. The pres- sure keeps the IPG strip in contact with the IPG strip holder electrodes. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 47 Carefully close the safety lid (1) by applying light downwards pressure, ensure the mechanism locks the lid. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 48 To inspect the run the light protective cover can be lifted without stopping the run. To open the safety lid press down the lid and the lock is released and the run is stopped. Close the lid and the run continues. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 49: Use The Ettan Ipgphor Cup Loading Manifold

    Position the manifold on Ettan IPGphor 3 Follow the instructions below to position the manifold on Ettan IPGphor 3. Step Action Clean and dry the Ettan IPGphor 3 platform before placing the manifold on the system. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 50 Action Position the manifold on the Ettan IPGphor 3 platform. The small T-shaped protrusion fits into a cutout section of the Ettan IPGphor 3 platform near the lid hinge. Ensure that the manifold is level by placing the round spirit level on the center of the manifold tray after it is placed on Ettan IPGphor 3.
  • Page 51 Use the insertion tool, wiggle the tool gently while pushing it down. Fill the cups with cover fluid to test for proper seating of the cups. Replace the cover fluid after 10 minutes. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 52 The electrode must be in contact with the wick. Swivel the cams into the closed position under the external lip of the tray. The electrodes should not be moved while the cams are in the closed posi- tion. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 53 IPG strip. Close the Ettan IPGphor 3 lid. • Program and run Ettan IPGphor 3 from its own control panel display. See Section 5.3 Perform a run from the control panel, on page 55...

  • Page 54: Start Up The Instrument

    In a situation where there is a risk of injury, turn off the instrument by switching the mains power switch (located on the rear panel) to position 0. Malfunctions must be rectified before Ettan IPGphor 3 Isoelectric Focusing System is restarted.

  • Page 55: Perform A Run From The Control Panel

    In this section Section See page 5.3.1 Ettan IPGphor 3 control panel functionality 5.3.2 Protocol parameters 5.3.3 Set protocol parameters 5.3.4 Isoelectric focusing from the control panel 5.3.5 Pause or stop a protocol Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 56: Ettan Ipgphor 3 Control Panel Functionality

    Programmable functions including: rehydration time, platform temperature, current limit, voltage limit for each step, voltage gradient or step and step duration. Control unit keys The following keys on the control panel are used to program Ettan IPGphor 3. COMMAND Function...
  • Page 57 5 Operation 5.3 Perform a run from the control panel 5.3.1 Ettan IPGphor 3 control panel functionality COMMAND Function keys Down arrow The down arrow arrow key decreases the value of the selected field or digit. Note: The exception is when the cursor is positioned to the left under the Step number S1-S9, the down arrow key changes the display to the next group of three protocol steps.
  • Page 58 5 Operation 5.3 Perform a run from the control panel 5.3.1 Ettan IPGphor 3 control panel functionality P r o t # 1 F i l e 1 R e h y d r a t e 0 : 0 0 H r @ 2 0 ˚ C I E F @ 2 0 ˚...

  • Page 59: Protocol Parameters

    0 produces 9until a parameter limit is reached. Ettan IPGphor 3 can be programmed in volt-hours (Vhr) or hours and minutes in step- n-hold or in gradient mode. Any values within the operating limits listed above can be used.

  • Page 60: Set Protocol Parameters

    The original label file occupies 6 of the 16 available characters. To change the label to a more descriptive name, such as 11 CM 3 to 10L requires scrolling through the alphanumeric options for each of the 11 digits in the name. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 61 Note: Maintaining the actual platform temperature at <18°C or >25°C might require placement of Ettan IPGphor 3 in a location of lower or higher than ambient temperature (e.g. a cold room). It may be necessary to operate the instrument in an air conditioned room to prevent condensation from collecting on the platform in humid environ- ments when the platform is run below 7°C ambient temperature.
  • Page 62 I E F @ 2 0 ˚ C 5 0 µ A / S t r i p 1 S t r i p s 0 S t e p s Press EDIT on the control panel to move to next screen. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 63 S 2 G r a d 2 0 0 0 V 1 : 0 0 H r s S 3 S t e p 8 0 0 0 V 2 : 0 0 H r s Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 64 S 2 G r a d 1 0 0 0 V 4 : 0 0 H r s S 3 S t e p 8 0 0 0 V 8 0 0 0 0 V h r Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 65 Make sure that the voltage field in all unused steps displays a value of 0, or that the value in the Hrs field is 00:00. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 66 5.3 Perform a run from the control panel 5.3.3 Set protocol parameters Exit edit mode Press EDIT to save any changes and exit the step edit mode. The protocol identifica- tion screen will appear. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 67: Isoelectric Focusing From The Control Panel

    Isoelectric focusing from the control panel Precautions WARNING Ettan IPGphor 3 is a high voltage instrument that can cause fatal electrical shock if the safety features are disabled. The safety lid must be securely closed before starting a protocol. Protocol steps Once the protocol begins, each step is carried out automatically, and instrument status for each step is indicated on the screen.
  • Page 68 3 s t e p s 4 : 0 0 H r If a rehydration period was programmed, the rehydration status screen will indicate the number of hours elapsed and the actual platform temperature: Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 69 S t e p V h r s E l a p s e d 5 0 0 V 4 0 µ A / S t r i p 0 . 0 W Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 70 5 0 0 V 4 0 µ A / S t r i p 0 . 0 W Ettan IPGphor 3 will display the volt-hours during the run as: • VhS, which indicates the total volt hours for that step, or •...
  • Page 71 5 0 µ A 8 : 0 0 H r To t a l P r e s s S t o p t o r e s e t The final instrument values are displayed. Press STOP, as indicated, to reset the instrument. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 72: Pause Or Stop A Protocol

    To continue the protocol, press the START key. To cancel the protocol, press the STOP key again. This will cause the instrument to respond as though the end of an uninter- rupted protocol had been reached as described above. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 73: Run A Pre-Programmed Protocol From The Pc

    5 Operation 5.4 Run a pre-programmed protocol from the PC Run a pre-programmed protocol from the PC In this section Section See page 5.4.1 Setting up a run 5.4.2 Perform a run Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 74: Setting Up A Run

    The HV ON indicator on the front panel should not light. Main screen and buttons Part Function Instrument selection buttons Protocol mode buttons Instrument control buttons Info button Protocol view button Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 75 Right-click on Ettan IPGphor 3 control software and choose Run as admin- istrator. Select the desired Ettan IPGphor 3 by clicking one of the four instrument selection buttons (Instr1 - Instr4). The identity of the instrument should now be shown below the buttons. Up to four instruments can be run simultaneously with the software.
  • Page 76 Select the Run settings & details tab by clicking the tab. Set experimental conditions, that is Strip length, pH range and Number of strips, by clicking the corresponding button. The software now selects an optimized Cytiva protocol for the specified experimental parameters. Edit the selected protocol Select the Protocol view in the Run settings &...
  • Page 77 Change protocol file name and protocol directory folder, if desired. Note: If the name of an edited protocol will be overwritten it will be lost. Download protocols to a specific position Ettan IPGphor 3 can store up to ten protocols. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 78 5.4 Run a pre-programmed protocol from the PC 5.4.1 Setting up a run Step Action To download a protocol to Ettan IPGphor 3 at another position than position 1, select menu Protocol →Transfer protocol. and select desired Instru- ment position (1-10). Click OK.

  • Page 79: Perform A Run

    Follow the instructions below to pause a run. Step Action Click Pause button to pause an ongoing run. The run is now paused which is indicted by the word Paused below the instrument control buttons. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 80 Display graphics and data Ettan IPGphor 3 control software provides graphics of the data (Voltage, Volt-hours and Current) as it is retrieved from Ettan IPGphor 3. The user can monitor and display the progress of any individual run from any one of four different instruments being run in parallel.
  • Page 81 Click the left of the two buttons at the lower right corner of the tab window to select the Info and data view. The main running parameters are now displayed. Vh or Time is indicated depending on what is selected to define the length of the current step. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 82 Click another instrument selection button for monitoring runs on other instruments controlled from the software. This way it is possible to monitor up to four parallel runs. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 83 5.4 Run a pre-programmed protocol from the PC 5.4.2 Perform a run Capture run data Run data is acquired from the Ettan IPGphor 3 Isoelectric Focusing Unit automatically every minute during a run, and the data is presented in a graph and in numerical displays.

  • Page 84: Maintenance

    WARNING Ettan IPGphor 3 covers must not be opened by the user. It contains electrical circuits which can give a lethal electric shock. Service and planned maintenance should be performed by personnel author- ized by Cytiva.
  • Page 85 6 Maintenance NOTICE Keep the instrument dry and clean. Wipe regularly with a soft damp tissue and, if necessary, a mild cleaning agent. Let the instrument dry completely before use. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 86: Cleaning Before Planned Service

    Health and safety declaration forms Health and safety declaration forms are available for copying or printing in the Refer- ence information chapter of this manual, or on digital media supplied with the user documentation. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 87: Cleaning Ettan Ipgphor 3

    IPGphor Strip Holder Cleaning Solution has been specifically formulated for removing protein deposits. It will not damage the strip holder or manifold. IPGphor Strip Holder Cleaning Solution (29011927) can be ordered in 1 liters bottles from Cytiva. Clean ceramic strip holders/manifold NOTICE The ceramic strip holders and manifold are very brittle and fragile.
  • Page 88 6 Maintenance 6.2 Cleaning Ettan IPGphor 3 The Ettan IPGphor 3 strip holders and manifold are made from a thermally conductive aluminum oxide ceramic. They have been surface-treated to minimize adsorption and interaction with proteins. The surface coating is mechanically robust and can with- stand temperatures of up to 300°C, however it can be damaged by prolonged exposure...
  • Page 89 Do not use any organic solvents during cleaning. Clean electrodes NOTICE Avoid scratching the electrode surfaces. Wash the electrode assemblies with the IPGphor Strip Holder Cleaning Solution. Rinse thoroughly with water then deionized water and allow to air dry. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 90: Replace Fuses

    T2.5 AL 250V, 5 × 20 mm. Fuse compartment Part Function Mains power switch Hinged cover Slot Casette end Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 91 Plug the power cord into the unit and turn the mains power switch on. If the instrument does not start when power is switched on after replace- ment of mains fuse(s), call Cytiva service. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 92: Troubleshooting

    Note the component that failed and press the START key to continue through the diagnostic component failure program. Call your local Cytiva service for further information on how to remedy the failure. An electric arc has been detected. The instru- ment will reduce the voltage setting 500 V auto- matically.
  • Page 93 Excessive charged material in the sample or rehydration buffer lead to electro-endosmosis which will dry out the middle of the strip, possibly leading to arcing and burning at these points. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 94: Reference Information

    This chapter lists the technical specifications of Ettan IPGphor. The chapter also includes ordering information, and Health and Safety Declaration form for service. In this chapter Section See page Technical specifications Recycling information Regulatory information Ordering information Health and Safety Declaration Form Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 95: Technical Specifications

    Programmable parameters Rehydration time, platform temperature, maximum current limit per strip, voltage limit in each step, voltage step or gradient, and step duration. Protocol capacity 10 protocols, with up to 9 steps each. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 96 Item/Parameter Specification USB Type-C port 1200 or 9600 baud, 8 data bits 1 stop bit, no parity, no flow control. 9600 baud is manda- tory for connection with Ettan IPGphor 3 control software. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 97: Recycling Information

    Waste electrical and electronic equipment must not be disposed of as unsorted munic- ipal waste and must be collected separately. Contact an authorized representative of the manufacturer for information concerning the decommissioning of the equipment. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 98: Regulatory Information

    See page 8.3.1 Contact information 8.3.2 European Union and European Economic Area 8.3.3 Great Britain 8.3.4 Eurasian Economic Union (Евразийский экономический союз) 8.3.5 North America 8.3.6 Regulatory statements 8.3.7 Declaration of Hazardous Substances (DoHS) Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 99: Contact Information

    The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 100: European Union And European Economic Area

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 101: Great Britain

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 102: Eurasian Economic Union (Евразийский Экономический Союз)

    Telephone: +7 985 192 75 37 E-mail: rucis@cytiva.com Информация о производителе и импортере В следующей таблице приводится сводная информация о производителе и импортере, согласно требованиям Технических регламентов Таможенного союза и (или) Евразийского экономического союза. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 103 Member States of the Customs Union of the Eurasian Economic Union Данный знак о Евразийском соответствии указывает, что изделие одобрено для использования на рынках государств-членов Таможенного союза Евразийского экономического союза Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 104: North America

    Note: The user is cautioned that any changes or modifications not expressly approved by Cytiva could void the user’s authority to operate the equip- ment. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.

  • Page 105: Regulatory Statements

    When used in a residential environment, there is a concern of radio interference. 유의사항 A급 기기 (업무용 방송통신 기자재) 이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받 은 기기 로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습 니다. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 106: Declaration Of Hazardous Substances (Dohs)

    EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning. Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 107 Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB/T 26572 • Data listed in the table represents best information available at the time of publication. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 108: Ordering Information

    Cover fluid can be ordered using CAS number 8012-95-1. More information For more details on ordering information and for information about spare parts and accessories, visit cytiva.com or contact your local Cytiva representative. Ettan IPGphor 3 Operating Instructions 28963551 AF...

  • Page 109: Health And Safety Declaration Form

    Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
  • Page 110 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.

  • Page 111: Index

    Electrode area, 20 Manufacturing information, 99 Electrode pads, 42 Electrode paper wicks, 52 Electrodes, 52, 89 Notes and tips, 6 Clean, 89 Emergency shutdown, 16 On site service, 109 Operating environment, 28 FCC compliance, 104 Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 112 Capture run data, 83 Edit protocol, 76 Monitor a run, 80 Pause a run, 79 Save protocol, 77 Select instrument, 75 Set up run, 76 Start run, 79 Stop a run, 80 Store data, 83 Ettan IPGphor 3 Operating Instructions 28963551 AF...
  • Page 113 Page intentionally left blank...
  • Page 114 Cytiva and the Drop logo are trademarks of Life Sciences IP Holdings Corp. or an affiliate doing business as Cytiva. CyDye, Ettan, Immobiline, and IPGphor are trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva.

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