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Summary of Contents for cytiva TE62
- Page 1 TE62 Standard Transfer Tank Operating Instructions Original instructions cytiva.com...
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Page 2: Table Of Contents
European Union and European Economic Area ................40 8.3.3 Eurasian Economic Union Евразийский экономический союз ....................41 8.3.4 Declaration of Hazardous Substances (DoHS) ................43 Ordering information ........................... 45 Health and Safety Declaration Form ...................... 46 Index ........................... 48 TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 3: Introduction
About this chapter This chapter contains important user information, descriptions of safety notices, and intended use of the Transphor instrument. In this chapter Section See page About this manual Important user information TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 4: About This Manual
Transphor instrument. Illustrations The images and annotations in this document are for illustrative purposes only. The configuration of individual products may vary, and therefore illustrations may not reflect the actual system delivered. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 5: Important User Information
A note is used to indicate information that is important for trouble-free and optimal use of the product. Tip: A tip contains useful information that can improve or optimize your proce- dures. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 6: Safety Instructions
Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved. In this chapter Section See page Safety precautions Labels Emergency procedures TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 7: Safety Precautions
It is important not to proceed until all stated conditions are met and clearly understood. NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 8 Do not damage the power supply cord by bending, twisting, heating or allowing it to become pinned under the equipment. Using damaged power cords could result in fire or electric shock. If the power supply cords are damaged, contact your local Cytiva representative for replacements. WARNING The safety lid must be in place before connecting the power leads to a power supply.
- Page 9 A fume hood or similar ventilation system shall be installed when flammable or noxious substances are used. Installing and moving the product CAUTION Turn off the power switch and remove connecting cables before moving the equipment. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 10 WARNING Never exceed the operating limits stated in this document and on the system label. Operation of the product outside these limits can damage equipment and cause personal injury or death. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 11 Never introduce anti-freeze or any organic solvent into any part of the instrument. Organic solvents will cause irreparable damage to the instrument. CAUTION Buffer above the maximum buffer level line may cause corrosion of the electrical contacts. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 12 Transphor is properly decontaminated and sanitized before main- tenance or service. WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. WARNING Disconnect power. Always disconnect power from the instru- ment before performing any maintenance task.
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Page 13: Labels
Do not open any covers or replace parts unless specifically stated in the user documentation. Warning! High Voltage. Always make sure that the system is disconnected from electric power before removing the lid. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 14: Emergency Procedures
To restart the run after an emergency shutdown or power failure, follow these steps: Step Action Make sure all connections are in place. Start the power supply as described in the power supply's User Manual. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 15: System Description
About this chapter This chapter gives an overview of the Transphor, and a brief description of its function. Illustration of the instrument The illustration below shows the main parts of the instrument. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 16 Tank fill levels Coolant ports (2 ×) Coolant safety valve Illustration of the transfer stack assembly The illustration below shows the main parts of the instrument. Part Function Sponge (3 mm) Blotting paper Membrane TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 17 3 System description Part Function Blotting paper Sponge (3 mm) for gels > 1.5 mm Sponge (6 mm) for gels ≤ 1.5 mm TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 18: Installation
Inspect all visible parts for damage or missing pieces. If any damage is observed, record this on the receiving documents and inform your Cytiva representative. Make sure to keep all packing material for damage claims or to use should it become necessary to return the unit. -
Page 19: Operation
Circulate coolant through the heat exchanger to minimize heating. Overheating will cause irreparable damage to the unit! Do not connect the heat exchanger to a water tap or any coolant source where the water pressure is unregulated. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 20 CAUTION Never introduce anti-freeze or any organic solvent into any part of the instrument. Organic solvents will cause irreparable damage to the instrument. In this chapter Section See page Preparations Assembly Electrotransfer TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 21: Preparations
Attach one end of each length of tubing to a heat exchanger port. Attach the free ends of each length of tubing to the circulator bath ports; one to the inlet and the other to the outlet. Secure the connections with the hose clamps. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 22 Place the instrument on a magnetic stirrer. Fill transfer buffer to the START FILL LEVEL line. This requires approxi- mately 3.8 L. Set the stirrer to low-medium. This creates buffer circulation without forcing buffer through the assembled cassettes. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 23: Assembly
Excess materials may result in cassette damage. NOTICE Take great care in removing all air bubbles at each step because the presence of air bubbles, especially between the membrane and gel, blocks transfer. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 24 (above the gel) with a sheet of blotting paper. If you remove the bottom sponge (below the membrane), substitute at least two sheets of blotting paper to create space between the membrane and the cassette panel. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 25 See Illustration of the instrument, on page 15, and Assemble transfer cassette, on page CAUTION Buffer above the maximum buffer level line may cause corrosion of the electrical contacts. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 26: Electrotransfer
Buffer Towbin 1x TBE or 1x TAE Current (A) 0.8 to 1.0 0.9 to 1.0 Voltage (V) 70 to 80 Transfer time ~1 h ~1 h Coolant temperature 10°C 10°C or less TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 27 Tip: It is a good idea to stain the gel to determine the completeness of the transfer. Air dry the membrane, or follow the instructions of your protocol to stain it. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 28 Discard the blotting paper, but reuse the sponges. Rinse the unit immediately after use. See General cleaning procedure, on page Note: Do not store used buffer in the transfer tank. Chill buffer to 10°C before reuse. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 29: Maintenance
WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. NOTICE Cleaning. Keep the exterior of the instrument dry and clean. Wipe regularly with a soft damp tissue and, if necessary, a mild cleaning agent. - Page 30 Health and safety declaration forms Health and safety declaration forms are available for copying or printing in the Refer- ence information chapter of this manual, or on digital media supplied with the user documentation. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 31: Troubleshooting
If the suggested actions in this guide do not solve the problem, or if the problem is not covered by this guide, contact your Cytiva representative for advice. Problems with incomplete transfer... - Page 32 Lower pH (< 6 to 7) increases the positive charge on proteins; higher pH (> 6 to 7) increases the negative charge on proteins. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 33 Error description Corrective action Chemical parameters Fix or crosslink the molecule onto the membrane according to the require- ments of the nucleic acid, protein, or membrane type. Prepare protein transfer buffer without SDS. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 34 Check both membranes for protein(s). Check if too much sample is available for the binding surface area by applying two membranes instead of one. If “blow through” occurs, reduce the sample load. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 35: Reference Information
The chapter also includes ordering information, and Health and Safety Declaration forms for service. In this chapter Section See page Specifications Recycling information Regulatory information Ordering information Health and Safety Declaration Form TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 36: Specifications
Installation category Environmental requirements Parameter Requirement Allowed location Indoor use only Ambient temperature, operation 4°C to 40°C Max. relative humidity, operation Up to 80% RH Altitude, operation Up to 2000 m Pollution degree TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 37: Recycling Information
Waste electrical and electronic equipment must not be disposed of as unsorted munic- ipal waste and must be collected separately. Please contact an authorized representa- tive of the manufacturer for information concerning the decommissioning of the equipment. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 38: Regulatory Information
This section lists the regulations and standards that apply to the product. In this section Section See page 8.3.1 Contact information 8.3.2 European Union and European Economic Area 8.3.3 Eurasian Economic Union Евразийский экономический союз 8.3.4 Declaration of Hazardous Substances (DoHS) TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 39: Contact Information
The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 40: European Union And European Economic Area
• used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 41: Eurasian Economic Union
Telephone: +7 499 609 15 50 E-mail: rucis@cytiva.com Информация о производителе и импортере В следующей таблице приводится сводная информация о производителе и импортере, согласно требованиям Технических регламентов Таможенного союза и (или) Евразийского экономического союза. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 42 Member States of the Customs Union of the Eurasian Economic Union Данный знак о Евразийском соответствии указывает, что изделие одобрено для использования на рынках государств-членов Таможенного союза Евразийского экономического союза TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 43: Declaration Of Hazardous Substances (Dohs)
This symbol indicates that this electrical and electronic product does not contain any hazardous substances above the maximum concentration value established by the Chinese standard GB/T 26572 , and can be recycled after being discarded, and should not be casually discarded. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 44 Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB/T 26572 • Data listed in the table represents best information available at the time of publication. TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
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Page 45: Ordering Information
8 Reference information 8.4 Ordering information Ordering information For product codes and information about how to order, please see cytiva.com. TE62 Standard Transfer Tank Operating Instructions 29281927 AB... -
Page 46: Health And Safety Declaration Form
Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival. - Page 47 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.
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Page 48: Index
System label, 13 Labels, 13 Description, 13 Safety labels, 13 System label, 13 Transfer, 26 parameters, 26 Manufacturing information, 39 Transfer cassette, 25 installation, 25 Transfer stack, 16 illustration, 16 Notes and tips, 5 TE62 Standard Transfer Tank Operating Instructions 29281927 AB... - Page 49 Index Transphor , 15 instrument main parts, 15 Troubleshooting, 31, 33 diffuse band patterns, 33 incomplete transfer, 31 inefficient binding, 33 Unpacking, 18 Unpacking procedure, 18 TE62 Standard Transfer Tank Operating Instructions 29281927 AB...
- Page 50 © 2020–2021 Cytiva All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.
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