AND PT Series Instruction Manual
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AND PT Series Instruction Manual

Pipette accuracy tester
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Pipette Accuracy Tester
PT-Series
AD-4212B-PT
AD-4212A-PT
FX-300i-PT
INSTRUCTION MANUAL
1WMPD4001794A

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  • Page 1 Pipette Accuracy Tester PT-Series AD-4212B-PT AD-4212A-PT FX-300i-PT INSTRUCTION MANUAL 1WMPD4001794A...
  • Page 2 A&D Company, Limited. The contents of this manual and the specifications of the instrument covered by this manual are subject to change for improvement without notice.

  • Page 3: Table Of Contents

    CONTENTS 1. INTRODUCTION ..........................2 1-1 Unpacking the Tester.......................... 2 1-2 Description of Each Component......................3 1-3 WinCT-Pipette, Software for Pipette Accuracy Testing............... 4 1-4 Function of the Evaporation Trap ....................... 5 1-5 Description of a Sample Cup......................5 2. ASSEMBLING THE TESTER......................6 2-1 AD-4212B-PT / AD-4212A-PT ......................

  • Page 4: Introduction

    This manual describes how the PT series Pipette Accuracy Tester works and how to get the most out of it in terms of performance. It includes how to assemble the tester, how to measure and how to use the software, WinCT-Pipette, for pipette accuracy testing.

  • Page 5: Description Of Each Component

    A chamber to prevent the evaporation of the dispensed test liquid (distilled water). (Refer to “1-4 Function of the Evaporation Trap”.) Sample cup (Two types: 5 mL and 30 mL) A measuring cup to contain the test liquid dispensed from a pipette, with a water-absorbent sheet installed, to prevent water droplets from remaining on the edge of the pipette tip.

  • Page 6: Winct-Pipette, Software For Pipette Accuracy Testing

    PC. Using the temperature of the test liquid and the barometric pressure that are previously entered, the PC calculates the conversion factor (Z factor) from a mass value to a volumetric value and converts the mass value into a volumetric value automatically, using the Z factor.

  • Page 7: Function Of The Evaporation Trap

    As shown in the illustration below, a water-absorbent sheet is rolled into a cylinder and is placed along the inner wall of the sample cup.

  • Page 8: Assembling The Tester

    Insert the sample cup into the holder. Sample cup holder (5 mL) When the 5 mL sample cup is used: Insert the sample cup holder (5 mL) into the sample cup holder (30 mL), and then Sample cup holder (30 mL) insert the sample cup...
  • Page 9 4. Install the lower part of the evaporation trap on the Lower part of the evaporation trap weighing unit, mating the tabs with the holes on the base. Hole 5. Attach the upper part of the evaporation trap to the Upper part of lower part, mating the tab with the notch.

  • Page 10: Fx-300I-Pt

    Insert the sample cup into the holder. Sample cup When the 5 mL sample cup is used: holder (5 mL) Insert the sample cup holder (5 mL) into the sample cup holder (30 mL), and then insert the sample cup Sample cup holder (30 mL)
  • Page 11 4. Install the lower part of the evaporation trap on the Lower part of the evaporation trap weighing unit, mating the tabs with the holes on the base. Hole 5. Attach the upper part of the evaporation trap to the Upper part of lower part, mating the tab with the notch.

  • Page 12: Precautions Before Use

    15°C to 30°C ±0.5°C with greater than 50% RH. Install the balance where it is not exposed to direct sunlight and it is not affected by air from heaters or air conditioners. Especially be careful when using the AD-4212B-PT. It responds even to very subtle air flow.

  • Page 13: Anti-Static Treatment

    4. ANTI-STATIC TREATMENT An anti-static treatment has been applied to the evaporation trap. Cleaning it with alcohol will reduce the effect of the treatment. If the evaporation trap has acquired a static charge, apply an anti-static agent or use the optional AD-1683 DC static eliminator to remove the static electricity.

  • Page 14: Connecting A Pc To The Balance

    6. CONNECTING A PC TO THE BALANCE To make a connection between a PC and the balance, use the RS-232C cable or use both the RS-232C cable and the USB converter. 6-1 Connecting a PC When a PC COM port is used When using the COM port of a PC to make a connection to the balance, use the accessory RS-232C cable.

  • Page 15: Checking Com Port Numbers

    The COM port for a personal computer is displayed as “Communications Port (COMx)”. In the example below, the personal computer has two COM ports. Therefore, two COM ports appear; “Communications Port (COM1)” and “Communications Port (COM2)” Change the WinCT-Pipette COM port number to that of the COM port used.

  • Page 16: Calibrating The Balance

    Before calibration, refer to “3. PRECAUTIONS BEFORE USE”. Especially when calibrating the AD-4212B-PT / AD-4212A-PT with a minimum weighing value of 0.01 mg / 0.1 mg, make sure that the installation environment is appropriate and the balance is warmed up for the appropriate duration. 7-1 AD-4212B-PT / AD-4212A-PT 1.

  • Page 17: Fx-300I-Pt

    6. Place the calibration weight on the sample cup holder (30 mL) to confirm that calibration has been completed successfully. NOTE The evaporation trap can be used as a breeze break. Calibration can be performed, using the weighing pan and the breeze break provided as accessories.

  • Page 18: Preparation Before Use

    Attach the upper part of the evaporation trap to the lower part and place the evaporation trap on the balance or weighing unit. It takes approximately 15 minutes for the humidity inside the evaporation trap to stabilize to about 85% RH.

  • Page 19: Using The Thermometer

    Before use One LR-44 battery is provided with the thermometer. It is for testing the performance of the thermometer and may have a limited life. Before use, install the battery as described in “Replacing the battery” below. Replacing the battery When the LCD becomes blurred, replace the battery as follows.
  • Page 20 3. Insert the sensor sheath into the water. Water 4. The sampling interval is 10 seconds. To speed this process, press and hold the ON/FAST button for a sampling interval of every 2 seconds. 5. As the thermometer responds to the water...
  • Page 21 Recalling the maximum and minimum temperatures stored in memory With the current temperature displayed, Current temperature each pressing of the MAX/MIX button displays the maximum and minimum temperatures in Press MAX/MIN turn. Maximum temperature Press MAX/MIN check maximum minimum temperature during measurement, reset the Minimum temperature values in memory before measurement.
  • Page 22 : 0°C to 50°C, 75% RH or less, non-condensing : φ2.80, approx. 110 mm Sensor sheath dimensions Dimensions/mass : 176 x 40 x 16 mm, approx. 25 g (including the battery and sheath protector) Standard accessory : Sheath protector, battery...

  • Page 23: Measurement

    Correctness of the Z factor, that is used to convert a mass value to a volumetric value, depends on the density by the water temperature. The barometric pressure, even if changed in the range between 950 hPa and 1050 hPa, influences measurements only by 0.01%.
  • Page 24 Vibration yields unstable measurement values. To reduce errors caused by vibration Install the balance in a stable place avoiding vibration and shock. Use a solid weighing table that is free from vibration. If the installation site is the second floor or higher, use the AD-1685 anti-vibration table.

  • Page 25: Ad-4212B-Pt / Ad-4212A-Pt

    0.01 mg (0.01 μL). With a volume of less than 10 μL, 0.001 mg (0.001 μL). Function table Functions related to pipette accuracy testing are as follows. Class Item and Parameter Description Fast weighing speed, sensitive value FAST Cond MID.

  • Page 26: Display Lock Function

    10-2 Display Lock Function To use this function, set “Display lock function ( ploc )” to “ON ( 1 )”. Why use this function This function is used to compensate time-elapsed errors caused by evaporation. Even when the evaporation trap is used, an evaporation of 0.05 mg per minute (0.05 μL per minute) will occur.

  • Page 27: Troubleshooting

    11. TROUBLESHOOTING Displayed values are unstable. The AD-4212B-PT / AD-4212A-PT has a minimum weighing value of 0.01 mg / 0.1 mg and is influenced by the measurement environment. Measures to take Use the accessory breeze break. Referring to “3. PRECAUTIONS BEFORE USE”, check the measurement environment for sources of drafts, vibration and static electricity.

  • Page 28: Specifications

    The AD-4212B-PT is equipped with a smart range function. When the minimum weighing value is set to 0.001 mg and the mass value exceeds 5.1 g, the minimum weighing value will switch to 0.01 mg, and when the mass value exceeds 31 g, the minimum weighing value will switch to 0.1 mg automatically.

  • Page 29: Options

    12-1 Options Name and components Model 5-mL sample cup (with a water-absorbent sheet installed) 5-mL sample cup 5 pcs. Lid for 5-mL sample cup 5 pcs AX-PT-01 Water-absorbent sheet for 5-mL sample cup 5 pcs (The sheet is installed in the cup before shipping.)

  • Page 30: Appendix

    13.APPENDIX The table below lists the relation between the ISO 8655 requirements and the PT series pipette accuracy testers. Pipette ISO 8655 requirements nominal Maximum permissible error Balance minimum volume weighing value Accuracy Repeatability μL (μL) ±% ±μL Pipette accuracy tester 0.05...

This manual is also suitable for:

Ad-4212b-ptAd-4212a-ptFx-300i-pt

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