AND BM Series Instruction Manual
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AND BM Series Instruction Manual

Pipette accuracy tester kit
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Pipette Accuracy Tester Kit
for the BM series
BM-014
INSTRUCTION MANUAL
1WMPD4002534C

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  • Page 1 Pipette Accuracy Tester Kit for the BM series BM-014 INSTRUCTION MANUAL 1WMPD4002534C...
  • Page 2 A&D Company, Limited. The contents of this manual and the specifications of the instrument covered by this manual are subject to change for improvement without notice.

  • Page 3: Table Of Contents

    CONTENTS 1. INTRODUCTION ..........................2 1-1 Unpacking the Tester ..........................2 1-2 Description of Each Component ......................3 1-3 WinCT-Pipette, Software for Pipette Accuracy Testing ...............4 1-4 Function of the Evaporation Trap......................5 1-5 Description of the Sample Cup ......................5 2. ASSEMBLING THE TESTER ......................6 2-1 Preparing to Assemble the Tester .......................6 2-2 Assembling the Tester .........................7 3.

  • Page 4: Introduction

    ISO 8655. This manual describes how the Pipette Accuracy Tester Kit works and how to get the most out of it in terms of performance. It includes how to assemble the tester, how to measure and how to use the software, WinCT-Pipette, for pipette accuracy testing.

  • Page 5: Description Of Each Component

    A container to keep a test liquid such as distilled water.  Thermometer (with a clamp) Measures the temperature of the test liquid.  RS-232C cable and USB converter Connects to a personal computer (PC) to transmit the test values to the PC.

  • Page 6: Winct-Pipette, Software For Pipette Accuracy Testing

    PC. Using the temperature of the test liquid and the barometric pressure that are previously entered, the PC calculates the conversion factor (Z factor) from a mass value to a volumetric value and converts the mass value into a volumetric value automatically, using the Z factor.

  • Page 7: Function Of The Evaporation Trap

    As shown in the illustration below, a water-absorbent sheet is rolled into a cylinder and is placed along the inner wall of the sample cup.

  • Page 8: Assembling The Tester

    Rotate the plate on the axis of the back edge. Rotate the plate on the axis of the front edge. Remove the plate from the chamber. Separation plate Hook Remove the weighing pan and so on (refer to the figure below) from the BM balance. ＢＭ－５００ ＢＭ－３００ ＢＭ－２５２ ､...

  • Page 9: Assembling The Tester

    2-2 Assembling the Tester BM-20 / 22 1 Install the center positioning boss and evaporation trap base on the BM balance. 2 Install the microfuge tube holder on the center positioning boss. 3 Insert the 5 mL sample cup into the microfuge tube holder.
  • Page 10 When the 30 mL sample cup is used: Insert the sample cup into the holder. When the 5 mL sample cup is used: Insert the 5 mL sample cup holder into the 30 mL sample cup holder, and then insert the sample cup. 5 mL sample cup...
  • Page 11 3 Install the lower part of the evaporation trap on the evaporation trap base. 4 Install the upper part of the evaporation trap on the lower part to complete assembly. Evaporation trap Evaporation trap (Upper part) (Lower part) Completed NOTE When the pipette nominal volume is 500 L or more, the 5 mL sample cup cannot be used to perform the series of ten measurements per volume recommended by ISO 8655.

  • Page 12: Precautions Before Use

    Install the balance where the measurement environment is appropriate. The best operating temperature is 15°C to 30°C ±0.5°C with a humidity of 50%RH or greater. Install the balance where it is not exposed to direct sunlight and it is not affected by air from heaters or air conditioners.

  • Page 13: Anti-Static Treatment

    If the evaporation trap has acquired a static charge, we recommend that you apply an anti-static agent or use the built-in static eliminator of the BM series to remove the static electricity. 5. INSTALLING WinCT-Pipette For detailed information on the WinCT-Pipette program, refer to “How to use WinCT-Pipette.pdf ”...

  • Page 14: Connecting A Pc To The Balance

    6. CONNECTING A PC TO THE BALANCE To make a connection between a PC and the balance, use the RS-232C cable or use both the RS-232C cable and the USB converter. 6-1 Connecting a PC When a PC COM port is used When using the COM port of a PC to make a connection to the balance, use the accessory RS-232C cable.

  • Page 15: Checking Com Port Numbers

    NOTE When the USB converter is used, check the COM port number with the USB converter connected. 1 Click [Start], [Settings] and [Control Panel]. 2 Double-click [System]. 3 Click [Hardware] and [Device Manager]. 4 Double-click [Port (COM & LPT)]. COM port numbers will be displayed.

  • Page 16: Calibrating The Balance

    7. CALIBRATING THE BALANCE For details on calibration, read the BM series instruction manual in addition to this manual. Before calibration, refer to “3. PRECAUTIONS BEFORE USE”. Because the BM series has an especially high weighing sensitivity, make sure that the installation environment is appropriate and the balance is warmed up for the appropriate duration, one hour or more.

  • Page 17: Preparation Before Use

    Attach the upper part of the evaporation trap to the lower part and place the evaporation trap on the balance or weighing unit. It takes approximately 15 minutes for the humidity inside the evaporation trap to stabilize to about 85%RH.

  • Page 18: Using The Thermometer

    8-4 Using the Thermometer Each part name Battery cover [ON/OFF] button [AUTO OFF] button [MAX/MINI] button Sheath holder Sheath Material: Main body, battery cover: Acrylonitrile-butadiene- styrene (ABS) Button: Rubber Display: Polycarbonate (PC) Sheath: SUS304 Sheath holder: Polycarbonate (PC) Sheath holder fitting: Iron (Nickel plating) Display Auto power off function Maximum value display...
  • Page 19 The battery cover that is not secured properly may result in water entering inside and damaging the thermometer. CLOSE...
  • Page 20 Measuring the temperature 1 Press the [ON/OFF] button to turn the power on. 2 Remove the sheath holder when it is attached to the sheath. 3 Measure the temperature by one of the following methods depending on the material to be measured. When measuring the liquid Immerse the sensor into the position where you would like to measure the temperature.
  • Page 21 When measuring the gas Place the sensor in the location where you would like to measure the temperature and wait until the display becomes stable. When measuring a high temperature atmosphere such as steam, use a shield to protect the display from high temperature.
  • Page 22 The thermometer automatically displays the current measurement temperature if no operation is made for 30 seconds while on the maximum or minimum temperature display To check the maximum and minimum temperatures for a new measurement, reset the memory before starting the measurement as described in “Resetting the maximum and minimum temperatures stored in memory”...
  • Page 23 Battery life : Approx.1 year (When used for one hour per day) Operating temperature and humidity : 0 to 40°C, 75%RH or less, No condensation (Excluding the sheath) Storage temperature and humidity : 0 to 50°C, 75%RH or less, No condensation External dimensions of the sheath : 2.8 mm (Tip: 2.2 mm, length 20 mm)

  • Page 24: Measurement

    Be sure to use the evaporation trap. Using the evaporation trap, evaporation of the test liquid can be reduced to 0.05 mg per minute (0.05 L per minute).  Use the display lock function of the BM series. For details, refer to “10-1 Display Lock Function”. Water temperature and barometric pressure Correctness of the Z factor, that is used to convert a mass value into a volumetric value, depends on the density by the water temperature.
  • Page 25 Vibration yields unstable measurement values. To reduce errors caused by vibration Install the balance in a stable place avoiding vibration and shock. Use a solid weighing table that is free from vibration. If the installation site is the second floor or higher, use the anti-vibration table.

  • Page 26: Function Setting Of The Balance

    10. FUNCTION SETTING OF THE BALANCE 10-1 Display Lock Function To use this function, set “Display lock function ( ploc )” to “ON ( on )”. Why use this function This function is used to compensate time-elapsed errors caused by evaporation. Even when the evaporation trap is used, an evaporation of 0.05 mg per minute (0.05 L per minute) will occur.

  • Page 27: Troubleshooting

     We recommend that you use the anti-vibration table (AD-1671) to avoid vibration.  We recommend that you use the built-in static eliminator of the BM series if the cause of the unstable values may be due to static charges.

  • Page 28: Options

    12. OPTIONS Name and components Model 5 mL sample cup (with a water-absorbent sheet installed) 5 mL sample cup 5 pcs. Lid for 5 mL sample cup 5 pcs AX-PT-01 Water-absorbent sheet for 5 mL sample cup 5 pcs (The sheet is installed in the cup before shipping.)

  • Page 29: Appendix

    13. APPENDIX The table below lists the relation between the ISO 8655 requirements and the pipette accuracy tester. Pipette ISO 8655 requirements nominal Maximum permissible error Balance minimum volume weighing value Accuracy Repeatability L (L) ±% ±L 0.05 0.05 BM-20 / 22 0.08...
  • Page 30 MEMO...
  • Page 32 3-23-14 Higashi-Ikebukuro, Toshima-ku, Tokyo 170-0013, JAPAN Telephone: [81] (3) 5391-6132 Fax: [81] (3) 5391-6148 A&D ENGINEERING, INC. 1756 Automation Parkway, San Jose, California 95131, U.S.A. Telephone: [1] (408) 263-5333 Fax: [1] (408)263-0119 A&D INSTRUMENTS LIMITED Unit 24/26 Blacklands Way, Abingdon Business Park, Abingdon, Oxfordshire OX14 1DY United Kingdom Telephone: [44] (1235) 550420 Fax: [44] (1235) 550485 A&D AUSTRALASIA PTY LTD...

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Bm-014

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