Sea-Bird Scientific SUNA V2 User Manual
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Sea-Bird Scientific SUNA V2 User Manual

Submersible ultraviolet nitrate analyzer
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Document No.
SUNASUNADeep
Release Date:
2022-05-24
Version:
G
Submersible Ultraviolet Nitrate Analyzer
425-643-9866
seabird.com
User manual
SUNA V2 (2k)

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Summary of Contents for Sea-Bird Scientific SUNA V2

  • Page 1 User manual SUNA V2 (2k) Submersible Ultraviolet Nitrate Analyzer Document No. SUNASUNADeep Release Date: 2022-05-24 Version: 425-643-9866 seabird.com...

  • Page 3: Table Of Contents

    Table of Contents Section 1 SUNA quick start guide ....................3 Section 2 Specifications ........................5 2.1 Mechanical........................... 5 2.1.1 Bulkhead connector......................5 2.2 Electrical............................5 2.3 Optical............................5 2.4 Analytical............................6 2.4.1 Nitrate measurement accuracy................... 6 2.4.2 Nitrate measurement precision................... 6 Section 3 Set up sensor and verify operation ................
  • Page 4 Table of Contents 7.3.4 Description of adaptive sampling..................36 7.3.5 Sensor calibration from manufacturer................36 7.4 Interferences and mitigation....................... 36 7.4.1 Uncharacterized species in sample...................36 7.4.2 Optically dense constituents....................36 7.4.3 Identification of interfering species..................36 7.4.4 Sensor function......................... 37 7.5 CDOM absorption........................37 7.6 Optional equipment........................

  • Page 5: Section 1 Suna Quick Start Guide

    Section 1 SUNA quick start guide This quick start guide gives the steps necessary to set up and make sure that the SUNA sensor operates and collects data before it is deployed. Delivered items— • the SUNA sensor, either standard or "deep" rated, with optional wiper, if ordered (std. depth only) •...
  • Page 6 SUNA quick start guide...

  • Page 7: Section 2 Specifications

    Section 2 Specifications 2.1 Mechanical Rated depth 2000 m Weight (in air) 1.8 kg Pathlength 10 mm Length 55.5 cm Displacement 1384 cm³ Diameter 5.7 cm Material anodized aluminum Temperature range, operation 0–35 °C Temperature range, dry storage -20–50 °C 2.1.1 Bulkhead connector Contact Deep...

  • Page 8: Analytical

    Specifications 2.4 Analytical This sensor is designed to measure the concentration of nitrate ions in water. Measurements are in units of micro molar (µM). This is converted into mg/L. Note that 1 µM nitrogen = 0.014007 mg/L nitrogen. 2.4.1 Nitrate measurement accuracy Table 1 10 mm pathlength Concentration range Class-based fresh water...

  • Page 9: Section 3 Set Up Sensor And Verify Operation

    Section 3 Set up sensor and verify operation 3.1 Install and start software Install the manufacturer-supplied software and make sure that the sensor is ready to test for functionality before configuration and deployment. If the sensor is so equipped, install the batteries if necessary.

  • Page 10: Verify Sensor Collects Data

    Set up sensor and verify operation 3.4 Verify sensor collects data 1. Push Start in the Dashboard. The "Connection Mode" shows "Acquisition." • Push OK so that the software is "Temporarily Enabling Transmit Real Time Setting..." The software shows the data as it is collected in the Time Series tab. •...

  • Page 11: Monitor Data In Spectra Graph

    Set up sensor and verify operation • To select a specific part of the data to zoom in on, pull the mouse pointer diagonally. • Push Auto Range to see the data for each selected parameter. The software adjusts the scale so that the data will always show. •...

  • Page 12: Monitor Data In Absorbance Graph

    Set up sensor and verify operation 1. Put a check in the box next to either or both the "Time Axis" or the "Range Axis" to enable the Zoom In or Zoom Out options. 2. Push Select Sensors either in the Spectra graph or in the Real Time Display tab to select the parameters to see on the graph.
  • Page 13 Set up sensor and verify operation 2. Select one or more files to copy to the PC. The manufacturer recommends that the user use a USB connection to move the files because it is much faster. 3. Push the <- arrow to start the move. The status shows at the bottom of the File Manager window.
  • Page 14 Set up sensor and verify operation...

  • Page 15: Section 4 Deployment And Recovery

    Section 4 Deployment and recovery 4.1 Deployment wizard The deployment wizard makes it easy to set up the sensor for a specific deployment. 1. Select the Operational Mode. Push Next. • Continuous: sensor collects data continuously. • Periodic: sensor collects data for a user-set time or number of samples at user- set interval.
  • Page 16 Deployment and recovery...

  • Page 17: Section 5 Transmit Data

    Section 5 Transmit data 1. Push Transmit Data in the Dashboard area. The Transmit Data window shows. • Information in the Memory Summary lets the user see the available data storage in the sensor. • "Bytes" is the amount of memory in use. •...

  • Page 18: Options To Process Data

    Transmit data 5.2 Options to process data Go to the Data menu to select how data is processed. • Use Reprocess Data to apply a different calibration or other setting to saved data. • Use Show Data From Sensorto show a graph of saved data. •...

  • Page 19: Nitrate Reprocessing Details

    Transmit data Note that the data files collected with SDI-12 (standard sensor) do not contain spectral data and cannot be reprocessed. 1. If necessary, start the software and supply power to the sensor. 2. Go to the Data menu, then select SUNA, then Reprocess Data. The Reprocessing Dashboard shows.
  • Page 20 "Temperature and Salinity Correction" is available if the sensor is calibrated for seawater and water temperature and salinity data ("T-S data") are collected. This data can come from frames if the sensor operates in APF mode (SUNA V2 deep only), or from an external file.

  • Page 21: Show Data From Multiple Sensors

    Transmit data 5. Put a check in either the "Single Output File" box or the "Graph Results" box in the Output Data Files area of the dashboard. • If the user processes more than one file, the output can be directed to a single file of output.
  • Page 22 Transmit data...

  • Page 23: Section 6 Maintenance

    Section 6 Maintenance W A R N I N G If the user thinks that a sensor has water in the pressure housing: Disconnect the sensor from any power supply. Put on safety glasses and make sure that the sensor is pointed away from the body and other people.

  • Page 24: Update Reference Spectrum

    Maintenance Separation of the rubber from the pins. Swelled or bulging rubber pins. 6. Use a silicone-based lubricant on each of the contacts of the bulkhead connector. The manufacturer recommends any of the products listed below. ™ • Spray Silicone Lubricant (3M ID# 62-4678-4930-3). Make sure to let it dry. ®...
  • Page 25 Maintenance • Isopropyl alcohol (IPA) ® • Parafilm wrap Notes ™ • Use only lint-free tissues, OPTO-WIPES , or cotton swabs to clean the optical windows. • Use the software to update the reference spectrum. • Use only clean DI water that has been stored in clean glassware. ®...
  • Page 26 Maintenance Figure 3 Optical area filled with DI water 5. Supply power to the sensor and start the software if necessary. 6. Make sure that the sensor operates in Continuous mode: push Settings, then in the General tab, select "Continuous" for the "Operational Mode." 7.

  • Page 27: Compare Reference Spectrum Files

    Maintenance 6.4 Compare reference spectrum files Compare the change between two reference spectrum files. The amount of change is related to the time interval between the updates and the amount of lamp use during that time. Note that this procedure is done automatically by the software when the user updates the reference spectrum for the sensor.
  • Page 28 Maintenance 6. Push Browse to find the firmware file that is saved on the PC. 7. Push Open. 8. Push Upload. It takes approximately 2 minutes for the software to be updated. The software disconnects the sensor.

  • Page 29: Section 7 Reference

    254 and 300 nm and Root Mean Square Error (RMSE) to measure the quality of the data. The file extension is .csv. • APF—Deep SUNA V2 only. Used for APEX floats. Contains the user-selected parts of the spectrum and other auxiliary sensors. The frame size is typically 300–400 bytes.
  • Page 30 Reference Table 2 Information in data files (continued) Header and serial number Date, year and day-of-year BS 4 Time, hours of day BD 8 Nitrate concentration, µM BF 4 Nitrogen in nitrate, mgN/L BF 4 Absorbance, 254 nm BF 4 Absorbance, 350 nm BF 4 Bromide trace, mg/L...

  • Page 31: Continuous And Fixed-Time Operation

    Reference 7.1.2 Continuous and fixed-time operation The sensor must regularly collect a dark spectrum measurement so that it has a baseline correction for changes in time or "drift" in the output of the spectrometer. The user can set up the sensor to collect a dark spectrum measurement on a time-based or measurement-based schedule.

  • Page 32: Periodic Operation

    Reference Stops the sensor data collection. Measure N The sensor gets "n" light data frames. If "n" is zero, the sensor gets one dark data frame. Timed N The sensor gets light data frames for "n" seconds. The sensor sends CTD data to be corrected for temperature and salinity. The sensor must be set up to do the correction and to process it.

  • Page 33: Analog Operation

    Counter, Status, and Fitting Errors—always zero. 7.1.6 Analog operation The SUNA V2 is optionally equipped at the manufacturer's facility with analog output capabilities so that the sensor can work with analog input data collection systems such as the SBE 19Plus V2, 25Plus, and 9Plus CTDs. A standard application integrates the voltage signal into the auxiliary port of a CTD to get a profile of conductivity, temperature, and nitrate vs.

  • Page 34: Terminal Program

    Reference These equations can also be written as: × V, and nitrate × I nitrate where— = (DAC - DAC ) / (V is the voltage scale coefficient = DAC × V is the voltage offset coefficient = (DAC - DAC ) / (I is the current scale coefficient = DAC...

  • Page 35: Input-Output Configuration Values

    Reference 4. Turn on the power supply. 5. Send one or more "$" commands to the sensor to see a prompt at the command line. The sensor shows "SUNA>" when it is ready to accept commands. 6. Type "get opermode" to see the current mode of operation for the sensor. The default value is Fixed Time.

  • Page 36: Data Collection Setup Values

    Reference Acquisition file duration (min) 0–1440 afiledur Data wavelength, low (nm) 210–350 wdat_low Data wavelength, high (nm) 210–350 wdat_hgh 7.2.2 Data collection setup values Parameter Possible values Default value Short name Operation mode continuous, fixedtime, periodic, polled, SDI12 fixedtime opermode Operation control duration, samples samples...

  • Page 37: Theory Of Operation

    (ppm), the mass of N relative to the mass of water. The SUNA V2 sensor measures dissolved nitrate and displays units in micromolar (µM) or milligrams of nitrogen per liter (mg/N/L). The SUNA V2 does not display milligrams of nitrate per liter (mg/L or mgNO /L ).

  • Page 38: Description Of Adaptive Sampling

    7.3.4 Description of adaptive sampling The SUNA V2 has a 256-channel spectrometer that integrates for a specific length of time, usually 300–500 ms, to maximize the signal while it collects data. When the sensor makes a measurement, the spectrograph collects UV light for the length of the integration period.

  • Page 39: Sensor Function

    If the absorption of the sample is high (default cut-off = 1.3 AU), the model can no longer be used effectively to fit parameters or calculate nitrate concentration. The SUNA V2 data output is an RMSE parameter that indicates the quality of the fit of the models to the absorption curves.

  • Page 40: Optional Equipment

    (~220 nm). Absorption: The data output of the SUNA V2 is the absorption at 350 nm and 254 nm (A350 and A254). These wavelengths are outside the nitrate absorption range and can be used to make an estimate of the impact of CDOM.

  • Page 41: Flow Cell

    Reference Figure 5 Copper anti-fouling guard 7.6.2 Flow cell Use the flow cell for moored deployments that require a pump. Attach the flow cell to the sensor with the manufacturer-supplied plastic clamp. The flow cell seals against the pressure housing of the sensor so that the water from the pump flows across the optical path of the sensor.
  • Page 42 Reference...

  • Page 43: Section 8 Software Reference

    Section 8 Software reference 8.1 Sensor dashboards The Dashboard is the main area from which to control and communicate with the sensor. Information about the status of the sensor is contained in the upper part of the window and is updated each time the sensor is connected to the software. Common settings •...

  • Page 44: Message Tab

    Software reference • RS232 only: "Enable Real Time Display"—Put a check in this box to see the data as it is collected in either the software, or if deployed, a terminal program. • "Enable Time Series Graph"—Put a check in this box to see user-selected data. Remove the check in the box to so that the graph is disabled and the data does not show.

  • Page 45: Sensor Menu

    Software reference 8.3 Sensor menu The Sensor menus have options for each sensor with which the software communicates. Refer to the sections on Sensor dashboards on page 41 for more information about these items. Advanced Sensor menu items Common • Erase Data lets the user erase all of the data stored in the sensor.

  • Page 46: Show Data From The Sensor

    Software reference b. Select the filters to apply to the data in Export Samples QAQC Filter (ECO V2 only.) c. Select either the "UTC" or "Local" time stamp in Format Options. 7. Push Browse to select the directory in which the exported data will be saved. 8.

  • Page 47: Other General Settings

    Software reference Maximize: increases the size of the selected tab to fill the software screen size. Float: unlocks the selected tab from the software screen so that the user can move it to other locations on the PC screen. Float Groups: the same as Float above, but all tabs that are open are unlocked so that the user can move them to other locations on the PC screen.
  • Page 48 Software reference...

  • Page 49: Section 9 Troubleshooting

    Section 9 Troubleshooting W A R N I N G Nitrate sensors use an ultraviolet (UV) light. Do not look directly at a UV light when it is on. It can cause damage to the eyes. Keep products that have UV light away from children, pets, and other living organisms.
  • Page 50 Troubleshooting Table 6 Communication Possible problem Possible solution Collected data is different from • Verify the clock settings in the sensor. transmitted data • Make sure that there is no extra averaging or data collection in the setup. • Verify the data output settings. Problems with sensor-to- •...

  • Page 51: Section 10 General Information

    Section 10 General information W A R N I N G This product can expose the user to chemicals with silica, crystalline (airborne particles of respirable size), which is known to the State of California to cause cancer and birth defects or other reproductive harm.
  • Page 52 General information...
  • Page 54 Sea-Bird Electronics 13431 NE 20th Street Bellevue WA 98005 U.S.A. (425) 643-9866 © Sea-Bird Electronics, Inc., 2022. All rights reserved. *SUNASUNADeep*...

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