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Opentrons GEN1 Instruction Manual

Heater-shaker module
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Heater-Shaker Module GEN1
Instruction Manual
Opentrons Labworks Inc.
Edition
GEN1, August 2022

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Summary of Contents for Opentrons GEN1

  • Page 1 Heater-Shaker Module GEN1 Instruction Manual Opentrons Labworks Inc. Edition GEN1, August 2022...

  • Page 2: Table Of Contents

    ­ – ­Included­Parts ­ – ­Thermal­Adapters ­ – ­Physical­Specifications ­ – ­Power­Consumption –­Shaking­Profile –­Temperature­Profile –­Liquid­Classes­and­Volumes –­Supported­Labware Setup and Maintenance ­ – ­In-app­Setup­Guide – Setup Steps ­ – ­Software­Control ­ – ­Maintenance Support ­ – ­Opentrons­Help­Center ­ – ­Contact­Opentrons ­ – ­Warranty...

  • Page 3: Product And Manufacturer Description

    Product and Manufacturer Description PRODUCT DESCRIPTION The­Opentrons­Heater-Shaker­Module­GEN1­provides­on-deck­heating­ and­orbital­shaking.­The­module­can­be­heated­to­95­°C,­and­can­ shake­samples­from­200­to­3000­rpm. MANUFACTURER DESCRIPTION Opentrons Labworks Inc 45-18­Ct­Square­W Long­Island­City,­NY­11101...

  • Page 4: Safety Information And Regulatory Compliance

    See the Setup Steps section of this manual or the on- screen instructions in the Opentrons App for details on how to anchor the module. SAFE USE SPECIFICATIONS Input and Output Connections The­Heater-Shaker­has­the­following­power­input­and­output­...
  • Page 5 Safety Information and Regulatory Compliance Environmental Conditions For­optimal­performance,­Opentrons­recommends­using­the­ Heater-Shaker­in­the­following­conditions: Environment:­Indoor­use­only ◾ Temperature: 20–25­°C ◾ Relative humidity: 80%­maximum,­non-condensing ◾ Altitude: Up­to­2,000­meters­above­sea­level ◾ Pollution degree: 2 ◾ The­Heater-Shaker­can­be­safely­transported­and­stored­at­ temperatures­of­−10­to­60­°C. Software Requirements The­Heater-Shaker­requires­version­6.1.0­or­newer­of­the­Opentrons­ App­and­OT-2­robot­server. You­can­download­the­Opentrons­App­for­Windows,­macOS,­or­ Ubuntu­at­https://opentrons.com/ot-app/.

  • Page 6: Led Status Light

    LED COLOR AND BEHAVIOR MODULE CONDITIONS Pulsing­red Module­is­heating Solid­red Module­is­holding­at­target­ temperature and/or Module­is­hot­to­the­touch­(>49­°C) Solid­white Module­is­on­and­idle and/or Module­has­been­deactivated­and­it­ is­not­hot­to­the­touch­(<49­°C) Pulsing­white Module­is­in­identify­mode­(to­ distinguish­between­ multiple­Heater-Shaker­Modules) Pulsing­amber Module­is­in­an­error­state Pulsing­red­and­amber,­ Module­is­in­an­error­state­ alternating Module­is­hot­to­the­touch­(>49­°C)­ Pulsing­red­and­white,­ Module­is­in­identify­mode­ alternating Module­is­heating LED­off Module­is­powered­off HEATER-SHAKER MODULE GEN1...
  • Page 7 Safety Information and Regulatory Compliance Temperature Safety During­normal­operation,­the­top­plate,­labware­adapters,­and­ labware­on­top­of­the­Heater-Shaker­can­reach­temperatures of up to 95 °C.­Touching­these­surfaces­while­they­are­hot­poses­the­risk­of­ burns.­ To­reduce­the­risk­of­burns,­always­check­the­color­of­the­status­light­ before­touching­the­module.­If­the­status­light is red,­the­module­may­ be­hot­to­the­touch.­Do not touch­the­module.­Use­the­Opentrons­App­ to­deactivate­the­heater­and­allow­the­module­to­cool­until­the­status­ light­is­solid­white. If­you­must­touch­a­module­that­is­powered­off,­and­you­are­unsure­ whether­it­is­hot­to­the­touch: If­you­can­do­so­without touching any other part of the module, ◾ press­the­power­button­to­activate­the­module­and­check­the­ color­of­the­status­light. If­you­cannot­safely­press­the­power­button,­allow­the­module­to­ ◾ cool­for­at­least­10­minutes­before­touching­it.

  • Page 8: Safety Warning Labels

    (>49 °C). STANDARDS COMPLIANCE The­Heater-Shaker­has­been­tested­and­found­to­be­in­compliance­ with­all­applicable­requirements­of­the­following­safety­and­ electromagnetic­standards:­ Safety IEC/EN­61010-1­Safety­Requirements­for­Electrical­Equipment­for­ ◾ ­­­­­­­­Measurement,­Control­and­Laboratory­Use IEC­61010-2-010­Requirement­for­Heating ◾ IEC­61010-2-051:2018­Particular­requirements­for­laboratory­ ◾ ­­­­­­­­equipment­for­mixing­and­stirring Electromagnetic Compatibility FCC­part­15­subpart­B­class­A ◾ IEC/EN­61326-1­EMC­Testing­of­Laboratory­Equipment ◾ Hazardous Substances: RoHS­compliant HEATER-SHAKER MODULE GEN1...
  • Page 9 Safety Information and Regulatory Compliance FCC Warnings and Notes Warning:­Changes­or­modifications­to­this­unit­not­expressly­ approved­by­Opentrons­Labworks­Inc.­could­void­the­user’s­authority­ to­operate­the­equipment.­ Note:­This­equipment­has­been­tested­and­found­to­comply­with­the­ limits­for­a­Class­A­digital­device,­pursuant­to­part­15­of­the­FCC­Rules.­ These­limits­are­designed­to­provide­reasonable­protection­against­ harmful­interference­when­the­equipment­is­operated­in­a­commercial­ environment.­This­equipment­generates,­uses,­and­can­radiate­radio­ frequency­energy­and,­if­not­installed­and­used­in­accordance­with­ the­instruction­manual,­may­cause­harmful­interference­to­radio­ communications.­Operation­of­this­equipment­in­a­residential­area­ is­likely­to­cause­harmful­interference­in­which­case­the­user­will­be­ required­to­correct­the­interference­at­his­own­expense. Note regarding FCC compliance:­Although­this­instrument­has­ been­tested­and­found­to­comply­with­Part­15,­Subpart­B­of­the­FCC­ Rules­for­a­Class­A­digital­device,­please­note­that­this­compliance­is­ voluntary,­for­the­instrument­qualifies­as­an­“exempted­device”­under­ 47­CFR­15.103(c),­in­regard­to­the­cited­FCC­regulations­in­effect­at­the­ time­of­manufacture. CISPR 11 Class A Caution: This­equipment­is­not­intended­for­use­in­residential­ environments­and­may­not­provide­adequate­protection­to­radio­ reception­in­such­environments.

  • Page 10: Product Specifications

    INCLUDED PARTS (1)­Power­Supply ◾ (1)­Power­Cable ◾ (1)­USB­Cable ◾ (1)­T10­Torx­Screwdriver ◾ (1)­T10­Torx­L­Key ◾ (1)­M3x7­Thermal­Adapter­Screw­(plus­spares) ◾ THERMAL ADAPTERS A­compatible­thermal­adapter­is­required­for­adding­labware­to­the­ Heater-Shaker­Module.­Adapters­can­be­purchased­directly­from­ Opentrons­at­https://shop.opentrons.com.­ Currently­available­Thermal­Adapters­include: Universal­Flat­Adapter ◾ PCR­Adapter ◾ Deep­Well­Adapter ◾ 96­Flat­Bottom­Adapter ◾ PHYSICAL SPECIFICATIONS All­specifications­are­for­the­module­as­shipped:­with­latches­in­the­ rest­position­and­without­labware­or­adapters. Dimensions:­152­mm­L­x­90­mm­W­x­82­mm­H ◾ Weight:­1.34­kg ◾ Composition:­CNC­aluminum­and­polycarbonate­plastic ◾ HEATER-SHAKER MODULE GEN1...

  • Page 11: Power Consumption

    Product Specifications POWER CONSUMPTION These­are­typical­power­consumption­values­measured­at­the­wall­ outlet.­Typical­power­consumption­is­when­the­module­is­maintaining­ temperatures­and­shake­speeds­in­the­normal­range.­The­maximum­ power­consumption­is­when­the­module­is­simultaneously­heating­at­ full­power­and­shaking­at­maximum­speed. Idle:­3­W Typical­consumption: Shaking: 4–11 W ◾ Heating:­10–30­W ◾ Heating and shaking: 10–40 W ◾ Maximum­consumption:­125–130­W SHAKING PROFILE Orbital diameter:­2.0­mm ◾ Orbital direction:­Clockwise ◾ Speed range:­200–3000­rpm­(some­labware­may­recommend­ ◾ a­lower­maximum­rpm) Speed accuracy:­±25­rpm ◾ TEMPERATURE PROFILE Temperature range: 37–95­°C ◾...

  • Page 12: Liquid Classes And Volumes

    Product Specifications LIQUID CLASSES AND VOLUMES For­further­information,­consult­the­most­recent­application­notes­ and­white­papers­available­on­the­Heater-Shaker­product­page­on­ the­Opentrons­website. SUPPORTED LABWARE NEST­96­Well,­0.1mL,­Round­Well,­V­Bottom,­Full­Skirt ◾ NEST­96­Well,­0.2mL,­Round­Well,­Flat­Bottom,­Full­Skirt ◾ NEST­96­Well,­Deep­Well,­2mL,­Square­Well,­V­Bottom,­Full­Skirt ◾ Thermo­Scientific­Armadillo­PCR­Plate,­96-Well,­Clear,­Clear­Wells ◾ Corning­384­Well­Plate­112­µL­Flat ◾ Custom­flat-bottom­labware­can­be­used­with­the­Universal­Flat­ Adapter.­If­you­need­assistance­creating­custom­labware­definitions­ for­the­Heater-Shaker,­submit­a­request. HEATER-SHAKER MODULE GEN1...

  • Page 13: Setup And Maintenance

    Setup and Maintenance IN-APP SETUP GUIDE For­interactive­setup­instructions­and­to­run­a­test­shake­with­your­ Heater-Shaker­Module,­connect­the­module­to­your­robot­(see­ Connecting­USB­&­Power­below)­and­either: Go­to­the­Pipettes and Modules­section­on­the­robot’s­device­ ◾ detail­page,­or Load­a­protocol­that­requires­the­module­and­go­to­Step 2: ◾ Module Setup­in­the­Setup­tab­of­the­protocol­run­page. SETUP STEPS Preparing the Module Orient the module so its ports face away from you. Make sure the anchors are not extended and are level with the module’s base.
  • Page 14 Slots 1, 3, 4, 6, 7, and 10 are supported. Place the module in the slot such that the power and USB ports are facing outward, i.e. to the left in slot 1, 4, 7, or 10; to the right in slot 3 or 6. HEATER-SHAKER MODULE GEN1...
  • Page 15 Setup and Maintenance Hold the module flat against the deck and turn the screws clockwise to extend the anchors. Check that the module is firmly attached by gently pulling up on it and rocking it from side to side.
  • Page 16 Using different screws can damage the module. Check the alignment of the thermal adapter. If properly attached, it will sit evenly on the module. Check that the adapter is firmly attached by rocking it back and forth. HEATER-SHAKER MODULE GEN1...
  • Page 17 Setup and Maintenance Connecting USB & Power The module requires two connections: 1.­ Connect­the­power­cable­to­the­power­supply­and­ the­module.­Route­the­cable­through­the­gap­at­the­ bottom­of­the­robot’s­side­window. 2.­ Connect­the­USB­cable­to­the­module­and­an­open­ USB­port­at­the­back­of­the­robot.­Use­the­cable­ holders­on­the­side­of­the­robot­to­keep­the­USB­cable­ out­of­the­way. Power on the robot and module, and launch the Opentrons App. If­you­successfully­connected­your­module,­it­will­appear­ in­the­Pipettes and Modules­section­on­your­robot’s­ device­detail­page.­From­there­you­can­control­the­ module’s­labware­latch­or­run­a­test­shake.

  • Page 18: Software Control

    Setup and Maintenance SOFTWARE CONTROL The­Heater-Shaker­Module­can­be­controlled­in­JSON­(Protocol­ Designer)­and­Python­protocols.­Running­these­protocols­ requires­version­6.1.0­or­newer­of­the­Opentrons­App­and­OT-2­ robot­server. The­Opentrons­App­displays­the­current­status­of­the­Heater- Shaker­and­can­also­control­the­module­outside­of­protocols.­ To­control­a­Heater-Shaker,­go­to­the­Devices­tab­and­select­ the­robot­that­it­is­connected­to.­Robots­with­a­connected­and­ powered­on­Heater-Shaker­will­have­a icon­under­the­Modules­ section­of­the­device­card. On­the­device­detail­page,­a­module­card­shows­the­current­ status­of­the­Heater-Shaker,­including: Whether­the­heater­is­heating,­cooling,­or­holding­at­target.­ ◾ A­warning­banner­will­appear­if­the­module­is­hot­to­the­ touch­(>49­°C). The­target­(if­set)­and­current­temperature ◾ Whether­the­shaker­is­speeding­up,­slowing­down,­or­ ◾ holding­at­target The­target­(if­set)­and­current­shake­speed ◾ Whether­the­labware­latch­is­open­or­closed ◾ HEATER-SHAKER MODULE GEN1...
  • Page 19 Setup and Maintenance Click­the­three-dot­menu­­­­­­to­reveal­the­available­controls­ for­the­Heater-Shaker.­You­can­control­the­heater,­shaker,­and­ labware­latch­independently.­You­can­also­view­information­ about­the­module,­including­its­serial­number­and­firmware­ version,­or­launch­the­in-app­attachment­guide.

  • Page 20: Maintenance

    Setup and Maintenance MAINTENANCE Device Lifetime The­Heater-Shaker­has­been­tested­for­3­years­of­full-time­operation­ (more­than­1000­total­hours)­without­maintenance.­ Service and Repair Users­should­not­attempt­to­service­or­repair­the­module­themselves.­ If­you­have­concerns­about­your­module’s­performance­or­require­ service,­please­contact­Opentrons­Support. Cleaning Before­cleaning­the­Heater-Shaker,­unplug­the­power­and­USB­cables­ and­remove­it­from­the­deck. All­outer­surfaces­of­the­Heater-Shaker­are­compatible­with: 10%­bleach­solution ◾ 70%­ethanol ◾ 99%­isopropyl­alcohol ◾ Spray­one­of­these­cleaners­onto­a­paper­towel­and­use­it­to­wipe­the­ exterior­surfaces­of­the­module.­Avoid­introducing­these­substances­ into­the­USB­port­or­power­port.­Never­douse­or­spray­the­module­ directly­with­cleaner. The­module­can­also­withstand­ultraviolet­(UV)­light,­limited­to­ standard­sterilization­procedures.­Do­not­expose­the­module­to­ constant­or­high-intensity­UV­light. HEATER-SHAKER MODULE GEN1...

  • Page 21: Support

    Support OPENTRONS HELP CENTER Answers­to­many­common­questions­are­available­on­the­Opentrons­ Help­Center­at­http://support.opentrons.com/.­ CONTACT OPENTRONS If­you­have­any­other­questions,­contact­support@opentrons.com.­ Please­have­the­serial­numbers­of­your­module­and­robot­available­ when­contacting­support. WARRANTY All­hardware­purchased­from­Opentrons­is­covered­under­a­1-year­ standard­warranty.­Opentrons­warrants­to­the­end-user­of­the­ products­that­they­will­be­free­of­manufacturing­defects­due­to­ part­quality­issues­or­poor­workmanship­and­also­warrants­that­ the­products­will­materially­conform­to­Opentrons’­published­ specifications.

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