Miltenyi Biotec UltraMicroscope Blaze User Manual
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Miltenyi Biotec UltraMicroscope Blaze User Manual

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Summary of Contents for Miltenyi Biotec UltraMicroscope Blaze

  • Page 1 UltraMicroscope Blaze™ Imaging System User manual...
  • Page 2 UltraMicroscope Blaze™ Imaging System may make copies solely for purposes of training personnel in the use and servicing of the unit within their business or organization. Maximal care has been taken by Miltenyi Biotec in the preparation of this user manual.
  • Page 3 UltraMicroscope Blaze™ Imaging System User manual Original instructions Issued: 2022-06 212526.02 Distributed by Manufactured by Miltenyi Biotec B.V. & Co. KG LaVision BioTec GmbH - a Miltenyi Biotec Company Friedrich-Ebert-Straße 68 Astastr. 14 51429 Bergisch Gladbach 33617 Bielefeld Germany Germany Phone +49 2204 8306-0...
  • Page 4 Before using the UltraMicroscope Blaze™ Imaging System, read the chapter Important safety information and all other information contained in this user manual, including any safety and operating instructions. Pay special attention to all warnings displayed on the instrument.

  • Page 5: Table Of Contents

    Content 1 Important safety information 2 Introduction 2.1 The UltraMicroscope Blaze™ Imaging System 2.2 Intended use 2.3 Instrument description 2.3.1 Front view 2.3.2 Rear view 2.3.3 Equipment 3 Installation 3.1 Components 3.2 Accessories 3.3 Consumables 3.4 Unpacking and installation 4 Switching on and off 4.1 Switching on the instrument...
  • Page 6 7.1.2 Optimizing the measurement settings 7.1.3 Capturing an image 7.2 Capturing a multi-color z-stack 7.3 Capturing a mosaic image 8 Maintenance 8.1 Servicing 8.2 Cleaning the instrument 8.3 Cleaning the cuvette 9 Technical data and specifications 9.1 Specifications 9.2 EU Declaration of Conformity 10 Technical support 11 Legal notes 11.1 Limited warranty...

  • Page 7: Important Safety Information

    • If it is necessary to cut the power supply, unplug the cable from the power outlet and contact an authorized Miltenyi Biotec service provider or Miltenyi Biotec Technical Support. • Check the instrument before each use for visible damage. • Do not use the instrument if it has been dropped or is damaged. Contact Miltenyi Biotec Technical Support immediately.
  • Page 8 Hazard levels Signal words are used to warn against hazardous situations and property damages. The following signal words are used in this user manual. or WARNING! indicates a hazardous situation that, if not avoided, could result in death or serious injury. or CAUTION! indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.
  • Page 9 • Inspect the safety labels and safety markings regularly and replace them if they are not legible or identifiable from a safe viewing distance. • Contact Miltenyi Biotec for replacement labels. Figure 1.1: Hazard areas and safety symbols on the front side of the UltraMicroscope Blaze™ Imaging System...
  • Page 10 Figure 1.2: Hazard areas and safety symbols on the top side of the UltraMicroscope Blaze™ Imaging System Electrical and thermal hazards ELECTRICAL HAZARD Electric shock, short circuit, overheating, fire, and explosion may lead to death or serious injury. Protective housing The housing of the instrument reduces the risk of an electric shock and short circuits.
  • Page 11 • Unplug the instrument before cleaning it. • Use only small amounts of cleaning agents on a soft cloth to wipe the instrument. Do not spray or pour liquid cleaning agents onto or into the instrument. Humid and dusty environments A change in the environmental temperature may cause condensation inside the instrument.
  • Page 12 • If parts of the instrument are damaged, unplug and do not use the instrument until damaged parts are replaced. Contact Miltenyi Biotec Technical Support, if necessary. • Follow the instructions in the datasheet for each consumable.
  • Page 13 Motorized turret The motorized turret moves while the instrument is in operation. • Keep away from the motorized turret while the instrument is in operation. • Do not obstruct the movement of the motorized turret. Optical radiation hazards LASER RADIATION Direct exposure to laser beam may lead to eye injury.
  • Page 14 Heavy instrument • Only use tables that support a weight of 250 kg. • Do not install the instrument yourself. • Only trained Miltenyi Biotec personnel or personnel authorized by Miltenyi Biotec is allowed to install the instrument. ERGONOMIC HAZARD Risk of tearing or straining muscles.
  • Page 15 Transportation The instrument should be transported with care in packaging specified by Miltenyi Biotec. Internal damage can occur if the instrument is subjected to excessive vibration or if it is dropped. If the instrument needs to be shipped back to the manufacturer for service, contact Miltenyi Biotec for instructions and packaging materials.

  • Page 17: Introduction

    Intended use The UltraMicroscope Blaze™ Imaging System is a fully automated light sheet microscope for imaging large or multiple samples. The sample may only be examined in the supplied cuvette. Unauthorized modifications of the cuvette or the instrument as a whole are not permitted for safety reasons.

  • Page 18: Instrument Description

    Instrument description 2.3.1 Front view Figure 2.1: Front view of the UltraMicroscope Blaze™ Imaging System 2.3.2 Rear view Figure 2.2: Rear view of the UltraMicroscope Blaze™ Imaging System...

  • Page 19: Equipment

    Figure 2.3: Detail view of the I/O ports and power switch 2.3.3 Equipment Figure 2.4: Jog wheel for moving the sample arm in three directions (x, y, z) and sensitivity...

  • Page 21: Installation

    Installation Components The Beam Combiner and the White Light Laser are delivered with lasers, lenses, and emission filters, as specified when ordering the instrument. Additional accessories (Table 3.2) and consumables (Table 3.3) developed specifically for the system are available separately. For details, visit www.miltenyibiotec.com, or contact your local sales representative.

  • Page 22: Accessories

    Alignment tool for UltraMicroscope Blaze™ Imaging System 150-001-813 Sample holder set for UltraMicroscope Blaze™ Imaging System 150-001-814 Cuvette for UltraMicroscope Blaze™ Imaging System 150-001-673 XXL cuvette for UltraMicroscope Blaze™ Imaging System 150-001-901 Table 3.2: Optional accessories available for the UltraMicroscope Blaze™ Imaging System...

  • Page 23: Consumables

    Protect the instrument from shock and vibration. Do not place vibrating devices, such as vortex mixers, on the same table as the UltraMicroscope Blaze™ Imaging System. This could impair the quality of the results. Only Miltenyi Biotec service personnel is allowed to unpack and install the instrument. Please contact Miltenyi Biotec Technical Support for further information.

  • Page 25: Switching On And Off

    Switch on the external laser module. The key switch is located on the laser module, refer to manufacturer documentation. Switch on the UltraMicroscope Blaze™ Imaging System. The power switch is located on the rear side of the instrument (Figure 2.2).
  • Page 26 Name Function Location Adjust window/level Adjusts the contrast based on the Live window region of interest in the live window ROI for active profile Defines a region of interest in the Live window live window Rayleigh ROI Switches on the light sheet focus Live window in the live window Show crosshair...

  • Page 27: Preparing The Instrument

    Preparing the instrument BIOLOGICAL HAZARD Contamination or infection may lead to death or serious injury, depending on the material used. • Take care when handling samples and reagents. • Wear personal protective equipment (such as gloves, safety glasses, etc.) as indicated in the safety data sheet for the particular substance.
  • Page 28 Use a disposable syringe to fill the cuvette with more imaging solution up to the notch on the side. 10 Press the button on the drawer to lift the cuvette. The LED goes out. 11 Push the drawer to close the cuvette.

  • Page 29: Laser Alignment

    (Figure 6.1): Screw z1 is used for aligning the left-hand sheet in the z- plane. Screws z2 and z4 are used for aligning the three beams of each light sheet. Figure 6.1: Bottom view of the UltraMicroscope Blaze™ Imaging System with the positions of the adjustable precision screws Setting the beam path LASER RADIATION Direct exposure to laser beam may lead to eye injury.
  • Page 30 Check if the instrument is switched on. Refer to chapter Switching on the instrument on page 25. Pull out the drawer as far as possible to lower the cuvette. Place the alignment tool on the sample arm. Ensure that the sample arm is in a centered position. Press the button on the drawer to lift the cuvette.
  • Page 31 10 Move the alignment tool until the beams on the right-hand side pass through the tool and form three thin lines on the other side of the pinhole foil. 11 Select the 1.1× objective lens (LVBT 1×) with 1.0× zoom and the imaging solution used under Optical Setup >...
  • Page 32 17 Set the Light Sheet Properties > Sheet Width to the maximum. 18 Select the Advanced tab in the Settings 1 window. Always start with the central beam on the right-hand side. It is the reference beam and cannot be adjusted by the user. Click Light Sheet Configuration > Enable only the central beam to activate the central beam.
  • Page 33 Adjusting the horizontal focus To adjust the horizontal focus, select the Calibration tab on the bottom of the Settings 1 window. Move the slider Light Sheet Focus > Offset Calibration until only one beam passes through the pinhole. Ensure that the horizontal focus is always in the center and the Light Sheet Properties > Sheet NA is at the maximum.

  • Page 34: Adjusting The Lateral Beams On The Right-Hand Side

    Adjusting the lateral beams on the right-hand side To adjust the lateral beams on the right-hand side, the horizontal focus must not be changed. Click Light Sheet Configuration > Disable the central beam in the Advanced tab to activate the lateral beams.

  • Page 35: Adjusting The Lateral Beams On The Left-Hand Side

    Click Light Sheet Selection > Select left light sheet in the Settings 1 window. Click Light Sheet Configuration > Enable only the central beam in the Settings > Advanced tab to activate the central beam. Ensure that the slider for the horizontal focus is still in the center and the NA is at maximum. If necessary, adjust the position.
  • Page 36 Click Light Sheet Configuration > Enable all three beams in the Advanced tab to activate all three beams. Draw a region of interest over the three beams passing the mask in the live window, and activate the intensity profile. Check the adjustment by clicking the Brain button under Scan Range in the Settings 2 window, and scroll with the mouse wheel to move the alignment tool up and down.

  • Page 37: Setting Up An Experiment

    Setting up an experiment Capturing a single-color z-stack 7.1.1 Aligning the sample LASER RADIATION Direct exposure to laser beam may lead to eye injury. • Do not stare into laser beam. • Do not insert reflective objects or tools into the cuvette. •...
  • Page 38 Select xyz-Table Z in the Devices list. Click the Autosave button at the end of the row under AS. It will turn green. Click the Autosave Settings to select the storage location. Select the appropriate objective Optical Setup > Objective, the zoom Optical Setup > Zoom and the imaging solution Optical Setup >...

  • Page 39: Optimizing The Measurement Settings

    12 Click and hold the Objective Lens Focus > Move Objective to Measurement button to move down the motorized turret. The objective will automatically stop. 13 Click the Show crosshair button on the right side of the live window to activate the crosshair. 14 Use the jog wheel to move the sample inside the cuvette until the surface of the sample is visible in the center of the crosshair.

  • Page 40: Capturing An Image

    The settings described may need to be adjusted when the zoom settings are changed. 7.1.3 Capturing an image Move in the z-direction to the starting position. Click Current Position > Set as Zero and Scan Range > Start > Take in the Settings 2 window. Move through the sample until you reach the end of the z-stack and click Scan Range >...
  • Page 41 Click the Autosave button at the end of the row under AS. It will turn green. Select the right excitation and emission wavelengths for your sample under Channel Setup in the Settings 1 window. The checked boxes are used. Move the slider under Laser Transmission Control while the filter is highlighted to set the laser power for each individual filter.

  • Page 42: Capturing A Mosaic Image

    Capturing a mosaic image Select Measurement Mode > Mosaic Acquisition in the Measurement Wizard window. Click the Mosaic tab in the xyz-Table Visual XY window. Double-click the green square representing the field of view. The square will turn gray. Click and drag the black dots at the edges to create a mosaic.
  • Page 43 Enter a percentage value to set an Overlap. To start the measurement, proceed as described under Capturing an image on page 40.

  • Page 45: Maintenance

    Maintenance Servicing Service the instrument every 12 months. Contact Miltenyi Biotec for support. Cleaning the instrument Use only small amounts of suitable cleaning agents on a soft cloth to wipe the instrument. Unplug the instrument. To disinfect, wipe accessible surfaces with 70% ethanol or isopropanol.

  • Page 47: Technical Data And Specifications

    Technical data and specifications Specifications The safety of the instrument may be compromised if used outside its specifications. UltraMicroscope Blaze™ Imaging System specifications General information Dimensions (w × d × h) 54 cm × 73 cm × 65 cm Weight...
  • Page 48 4–24 μm Width 1–20 mm Numerical aperture 0.0135–0.135 Focus positioning Dynamic Table 9.1: UltraMicroscope Blaze™ Imaging System specifications Camera specifications Detector 4.2 Mpx sCMOS camera 5.5 Mpx sCMOS camera Active pixels (w × h) 2048 × 2048 2560 × 2160 Pixel size 6.5 μm ×...

  • Page 49: Eu Declaration Of Conformity

    EU Declaration of Conformity This declaration of conformity is issued by the manufacturer: LaVision BioTec GmbH - a Miltenyi Biotec Company Astastr. 14 33617 Bielefeld Germany Product name: UltraMicroscope Blaze™ Imaging System Description: Laboratory equipment We hereby declare that the product conforms to the requirements of the following directives:...

  • Page 51: Technical Support

    Technical support For technical support, contact your local Miltenyi Biotec representative or Miltenyi Biotec Technical Support at Miltenyi Biotec headquarters: Miltenyi Biotec B.V. & Co. KG Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone: +49 2204 8306 3803 Email: technicalsupport@miltenyi.com Visit www.miltenyibiotec.com for local Miltenyi Biotec Technical Support contact information.

  • Page 53: Legal Notes

    Miltenyi Biotec group which supplied the product in effect at the date of purchase. Those terms and conditions of sale may vary by country and region. Nothing in this document should be construed as constituting an additional warranty.

  • Page 54: Trademarks

    Miltenyi Biotec’s warranty does not cover products sold AS IS or WITH ALL FAULTS, or which had its serial number defaced, altered, or removed, or any consumables or parts identified as being supplied by a third party. Miltenyi Biotec must be informed promptly if a claim is made under this limited warranty. If a material or...
  • Page 56 Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. Blaze, MACS, and the Miltenyi Biotec logo are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide.

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