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NEW BRUNSWICK SCIENTIFIC Innova CO-170 Manual To Operations

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Innova
®
CO-170
CO
Incubator
2
MANUAL NO: M1305-0050
Revision A
June 4, 2004
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 • 1-800-631-5417
Fax: 732-287-4222 • Telex: 4753012 NBSCO
Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com

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  • Page 1 CO-170 Incubator MANUAL NO: M1305-0050 Revision A June 4, 2004 NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005 Telephone: 1-732-287-1200 • 1-800-631-5417 Fax: 732-287-4222 • Telex: 4753012 NBSCO Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com...
  • Page 2 M1305-0050 Innova CO-170 CO Incubator User’s Guide...
  • Page 3 Fax: 49 (0)7022 32486 E-mail: sales@nbsnv-sa.be E-mail: sales@nbsgmbh.de CHINA THE NETHERLANDS New Brunswick Scientific Co., Inc. New Brunswick Scientific BV Room 1501, Xiangjiang Building, No. 18 Kerkenbos 1101, 6546 BC Nijmegen Lane 1265, Zhongshan Road (W) P.O Box 6826, 6503 GH Nijmegen Shanghai 200051, P.R.
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  • Page 5 Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed. New Brunswick Scientific Co., Inc. (NBS) is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by NBS.
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  • Page 7 Reproduction, adaptation, or translation without prior written permission from New Brunswick Scientific is prohibited. Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
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  • Page 9 W A R R A N T Y Every Instrument manufactured by the New Brunswick Scientific Co., Inc. is warranted to be free from defects in material and workmanship. This apparatus, with the exception of glassware, lamps and electrodes (where supplied),...
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  • Page 11: Table Of Contents

    CHAMBER ALARMS CREEN 3.8.1 Chamber Alarm System Function ..............32 ................34 DIAGNOSTIC CREEN ................... 35 3.10 HELP MENU CREEN ROUTINE MAINTENANCE ................... 37 ....................37 ENERAL OTES ..................... 37 AILY HECKS ....................37 ONTHLY HECK New Brunswick Scientific User’s Guide...
  • Page 12 APPENDIX A: HIGH TEMPERATURE DECONTAMINATION OPTION..49 ..........49 SING EMPERATURE ECONTAMINATION ..52 EMPERATURE ECONTAMINATION PTION WITH XYGEN ONTROL 9.2.1 Oxygen Sensor Removal ................52 9.2.2 Oxygen Sensor Replacement................ 52 INDEX........................55 M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 13: Introduction

    The Innova CO-170 CO incubator is microprocessor controlled and designed to ensure accurate and reliable operation. The unit incorporates a large, back-lit LCD display, touch- sensitive keypad and sophisticated control system that allows for easy programming, control and monitoring of the chamber conditions. The display also provides for graphical viewing of system data, on-screen messaging and help files to simplify operation and maintenance.
  • Page 14 Oxygen control (tri-gas) provides for conditions that require above- or below-ambient oxygen levels. These and other options and accessories provide for a uniquely flexible CO incubator capable of meeting the most demanding requirements. M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 15: Unpacking & Installation

    & 2.1 Inspection of Boxes After you have received your order from New Brunswick Scientific, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local NBS Sales Order Department or distributor immediately.

  • Page 16: Utilities

    15°C if the unit is being used at 37°C. Care should be taken to avoid placing the incubator in a position that may affect its performance, such as those listed below. M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 17: Installing The Feet

    A second incubator may be safely stacked on top of another identical incubator by using the custom designed stacking stand available as an accessory for the Innova CO-170. 2.5 Installing the Feet To ensure adequate airflow, the incubator feet must be installed. More than one person is required to perform this operation.
  • Page 18 Figure 1: Installing Shelf Racks 4. Install the three shelves, making sure that each shelf’s anti-tip tab is properly inserted onto each of the wire shelf rack guides (see Figure 2 below). Figure 2: Installing Shelves ANTI-TIP M1305-0050 Innova CO-170 CO Incubator User’s Guide...
  • Page 19 Install the HEPA AutoZero filter: 1. Press the AutoZero HEPA filter gently into the white plastic filter socket at the top of the rear panel of the incubator (see Figure 4 on the following page). New Brunswick Scientific User’s Guide...
  • Page 20 Figure 4: AutoZero HEPA Filter & Fitting M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 21: Operation

    The cluster of four arrowed Direction Keys will move the cursor around the screen and adjust values. file contains most of the information in this User Manual, together with HELP more detailed troubleshooting information. New Brunswick Scientific User’s Guide...

  • Page 22: Preparing For Operation

    3.3 Using the Humidity Tray & Humidity Control If humidification is required, the humidity tray should be filled with 1.5 liters of warm (∼ 37.0ºC) distilled water at this time. M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 23: Programming

    NOTE: If the incubator is supplied with the option of oxygen control, the setpoint for the oxygen level can be selected and changed like the temperature and CO setpoints. New Brunswick Scientific User’s Guide...

  • Page 24: User Access Code

    If the access code has been misplaced, you will be unable to make changes to your incubator’s settings. Contact customer service or your service representative for instructions on how to regain access to your incubator. M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 25: Referencing The Co 2 Sensor With Auto Zero

    See Section 7.1 for a list of options. Figure 6: USER SETTINGS Screen Use the & Direction Keys to move the cursor. Use ENTER Function Key to select an option. This section explains each of the screen features. USER New Brunswick Scientific User’s Guide...

  • Page 26: Date/Time Adjust

    Figure 7: PROGRAM CO AUTOZERO Screen NOTE: This procedure does not affect the internal chamber environment and will not affect your cell culture while being performed. M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 27: Datalogger

    See Section 7.1 for available options. To disable a feature, scroll to using the & direction keys, then press the function key. ENTER New Brunswick Scientific User’s Guide...

  • Page 28: Decontamination (Model Option)

    Power ON/OFF Chamber Temperature High/Low (programmed value) Level High/Low (programmed value) Supply Failure All System Alarms AutoZero (A/Z) Adjustments Auto Gain (A/G) Adjustments Oxygen and Relative Humidity (R/H) Alarms (where these options are installed) M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 29: Temperature Graph + Door Open Bar Chart

    When the data is compressed or decompressed, a light dotted line is displayed vertically on the screen to signify that the time axis is changing from 15-minute to 2½-hour increments or vice versa. A heavy dotted line is displayed when the unit is switched on. New Brunswick Scientific User’s Guide...

  • Page 30: Diagnostic Chamber Element Graph

    3.7.4 DIAGNOSTIC CHAMBER ELEMENT GRAPH This graph records chamber element temperature over time to assist troubleshooting. 3.7.5 DIAGNOSTIC DOOR GRAPH This graph records the door’s inner surface temperature over time to assist troubleshooting. M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 31: Diagnostic Door Element Graph

    Alternatively, the alarm system can be set to rearm only after the original temperature and CO setpoints have been achieved: 1. Choose the option ARM ALARMS WHEN AT SETPOINT: 2. Select for both TEMP New Brunswick Scientific User’s Guide...

  • Page 32: Chamber Alarm System Function

    Figure 12: CHAMBER ALARM Message After setpoints have been achieved for the first time, when the inner door is opened, the Alarm System is disabled; on closing the door, if selected, a programmable alarm delay starts: M1305-0050 Innova CO-170 CO Incubator User’s Guide...
  • Page 33 VISIBLE AND AUDIBLE CANCEL ALARM BY ALARMS ALARM - MESSAGE ON PRESSING ANY KEY CANCELLED SCREEN CANCEL HAVE CONDITIONS VISUAL AND AUDIBLE MESSAGE BY RECOVERED TO ALARMS CANCELLED, PRESSING ANY WITHIN +/- 0.1? MESSAGE LEFT ON SCREEN New Brunswick Scientific User’s Guide...

  • Page 34: Diagnostic Menu Screen

    This screen is mainly for technical service use, and can be used to troubleshoot the incubator systems before service is scheduled. This information allows technical support to optimize the service support required, and to shorten service time. Figure 15: DIAGNOSTICS Screen M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 35: Help Menu Screen

    If the user manual is misplaced, information about the CO incubator and its functions can always be found on-screen. Figure 16: HELP MENU Screen New Brunswick Scientific User’s Guide...
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  • Page 37: Routine Maintenance

    1.5 (or optional 2.5) liters. Use warm water (approx. 37.0°C) to ensure a rapid return to optimum chamber conditions. After adding water, check that the humidity tray is pushed fully back against the rear wall. New Brunswick Scientific User’s Guide...
  • Page 38 We recommend that you perform a CO AutoZero prior to sampling (see Section 3.5). 3. We also recommend that you AutoZero the CO system at least once every 28 days to ensure that CO level is correct. M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 39: Cleaning & Disinfecting

    3. Remove all of the shelves, the humidity tray and the shelf racks. 4. Place the black protective cover over the CO sensor. Also protect any additional sensors, such as Oxygen or Humidity, with the cover(s) supplied. New Brunswick Scientific User’s Guide...
  • Page 40 12. When the incubator has stabilized, carry out an AutoZero and reprogram the desired CO level. It may be necessary to open the glass door briefly if, after performing an AutoZero, the CO level is too high. M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 41: High Temperature Decontamination

    5.3 High Temperature Decontamination If your incubator is supplied with the high temperature decontamination option, follow the guidelines outlined in the Appendix (Section 9) for correctly and safely operating this option. New Brunswick Scientific User’s Guide...
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  • Page 43: Specifications

    Table 3: Specifications Innova CO-170 Incubator Specifications Digital programming via microprocessor control on 0.1°C increments. Measurement of chamber and door temperature via 4 RT* matched thermistors (sensitivity Temperature Management 0.01°C) Adjustable independent control of door heater “Out of Limits” temperature protection system independent of microprocessor control.
  • Page 44 Innova CO-170 Incubator Specifications Grounded Electrical Supply 100-120V 50/60 Hz Voltage: 220-240V 50/60 Hz 500 watts, Standard models Power: 1000 watts, High Temperature Decontamination models Energy to maintain 37°C: < 0.1 kwh Storage Temperature 10 - 50°C M1305-0050 Innova CO-170 CO Incubator User’s Guide...

  • Page 45: Options & Accessories

    P0628-5120 Stacking Stand with Casters, for double stacking P0628-5160 Under Bench Stand with Casters P0628-5180 Multi-position Shelf Racks (Max 8 positions) Standard InnovaLog PC-based data-collection Software (requires RS232) P0628-5600 Extra Large 2.5-liter Humidity Tray P0628-5150 New Brunswick Scientific User’s Guide...
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  • Page 47: Drawings & Tables

    DIAGNOSTICS Screen HELP MENU Screen 8.2 List of Tables Following is a key to the tables in this manual: Table Description Page Accessories Provided Utilities Specifications Decon Failure Codes & Descriptions Decon Failure Code Explanations New Brunswick Scientific User’s Guide...
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  • Page 49: Appendix A: High Temperature Decontamination Option

    Refer to Section 9.2 for the O sensor removal/reinstallation procedure. 4. To cancel the cycle, press . The incubator will cool down to the CANCEL programmed level where normal control takes over. See important NOTE on the following page. New Brunswick Scientific User’s Guide...
  • Page 50 DECONTAMINATION FAILED [CODE DECON: XX] codes are shown in Table 4a on the following page, and described in Table 4b. If this happens, please note the failure code and contact your service representative for advice. M1305-0050 Innova CO-170 CO Incubator User’s Guide...
  • Page 51 ( DECONTAMINATION COMPLETED OK If the chamber temperature is above the setpoint or the element temperature is greater than a factory-preset control point, cool-down will be entered until these conditions are satisfied. New Brunswick Scientific User’s Guide...

  • Page 52: High Temperature Decontamination Option With Oxygen Control

    9.2.2 Oxygen Sensor Replacement When the cool-down period of the decontamination cycle has finished, reinstall the Oxygen Sensor as follows: 1. Using the blanking plug as a tool, reinstall the Oxygen Sensor by turning it clockwise. M1305-0050 Innova CO-170 CO Incubator User’s Guide...
  • Page 53 Oxygen Sensor and cause it to malfunction. 2. Reconnect the sensor cable by plugging in the connector (grasp the white connector body, not the wire leads). 3. Replace the black plastic plug that covers the Oxygen Sensor to reduce heat loss. New Brunswick Scientific User’s Guide...
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  • Page 55: Index

    Daily, 37 Monthly, 37 Using the, 49 Cleaning, 39 with Oxygen Control, 52 Control Humidity Control, 14 Specifications, 43 Humidity Tray, 15 Condensation, 37 Installing the, 19 Minimizing, 14 Removing the, 19 Control Panel, 21, 23 New Brunswick Scientific User’s Guide...
  • Page 56 USER Screen, 25 User Settings, 25 Utilities, 16 Power Cord, 15 Power Frequency, 27 Pressure Setting, 22 Programmable CO AutoZero, 26 WARNING Programming, 23 Symbol for, 7 Warranty, 9 Weight Specifications, 43 Relative Humidity M1305-0050 Innova CO-170 CO Incubator User’s Guide...

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