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NEW BRUNSWICK SCIENTIFIC CO-150 Manual To Operations

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NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 • 1-800-631-5417
Fax: 732-287-4222 • Telex: 4753012 NBSCO
Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com
Model CO-150
CO
Incubator
2
MANUAL NO: M1310-0050
Revision A
April 17, 2004

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Summary of Contents for NEW BRUNSWICK SCIENTIFIC CO-150

  • Page 1 Model CO-150 Incubator MANUAL NO: M1310-0050 Revision A April 17, 2004 NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005 Telephone: 1-732-287-1200 • 1-800-631-5417 Fax: 732-287-4222 • Telex: 4753012 NBSCO Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com...
  • Page 2 M1310-0050 Model CO-150 CO Incubator User’s Guide...
  • Page 3 Fax: 49 (0)7022 32486 E-mail: sales@nbsnv-sa.be E-mail: sales@nbsgmbh.de CHINA THE NETHERLANDS New Brunswick Scientific Co., Inc. New Brunswick Scientific BV Room 1501, Xiangjiang Building, No. 18 Kerkenbos 1101, 6546 BC Nijmegen Lane 1265, Zhongshan Road (W) P.O Box 6826, 6503 GH Nijmegen Shanghai 200051, P.R.
  • Page 4 THIS PAGE IS INTENTIONALLY BLANK M1310-0050 Model CO-150 CO Incubator User’s Guide...
  • Page 5 Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed. New Brunswick Scientific Co., Inc. (NBS) is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by NBS.
  • Page 6 THIS PAGE IS INTENTIONALLY BLANK M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 7: Manual Conventions

    Reproduction, adaptation, or translation without prior written permission from New Brunswick Scientific is prohibited. Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
  • Page 8 THIS PAGE IS INTENTIONALLY BLANK M1310-0050 Model CO-150 CO Incubator User’s Guide...
  • Page 9 W A R R A N T Y Every Instrument manufactured by the New Brunswick Scientific Co., Inc. is warranted to be free from defects in material and workmanship. This apparatus, with the exception of glassware, lamps and electrodes (where supplied),...
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  • Page 11: Table Of Contents

    CLEANING & DISINFECTING ................31 ....................... 31 LEANING ......................31 ISINFECTING SPECIFICATIONS ...................... 33 OPTIONS & ACCESSORIES..................35 ........................35 PTIONS ..................35 VAILABLE CCESSORIES DRAWINGS & TABLES..................... 37 ....................37 IST OF RAWINGS ....................... 37 IST OF ABLES New Brunswick Scientific User’s Guide...
  • Page 12 INDEX ........................... 39 M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 13: Introduction

    The model CO-150 CO incubator is microprocessor-controlled and designed to ensure accurate and reliable operation. The unit incorporates a simple, door-mounted touch- sensitive keypad with two individual three-digit LED displays that allow for easy programming and monitoring of the chamber conditions.
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  • Page 15: Unpacking & Installation

    2.2 Unpacking WARNING! At least two people are required for to safely lift your CO-150. Disassemble the wooden shipping crate and remove the protective packing. Save the packing materials for possible future use, and be sure to save this User’s Guide for instruction and reference.

  • Page 16: Utilities

    • Directly under, beside or within the air-flow of heating or air- conditioning ducts, or other drafts; • Directly beside heat generating equipment such as a heater, an autoclave or oven; • Near the exhaust of heat- or cold-generating equipment (like a ULT freezer); M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 17: Installing The Feet

    It is not possible to put any other type of incubator or heavy apparatus on top, as the top cover is designed to support only the feet of another model CO-150. NOTE: There is no need to remove the top panel for normal maintenance or servicing, so the upper unit does not have to be moved when you are servicing the lower unit.
  • Page 18 (with installed HEPA filter) by attaching the tubing from the two-stage regulator (or in-line regulator) to the matching CO inlet on the rear of the incubator. 2. Use the tubing clips provided to eliminate CO leaks. M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 19: Operation

    AutoZero. Press this key simultaneously with the Down key to work in the Alarm system. NOTE: If you accidentally press both the Up and Down keys simultaneously, you will engage Engineering Mode: press the Programming key immediately to exit. New Brunswick Scientific User’s Guide...

  • Page 20: Preparing For Operation

    6. The chamber setpoints are pre-programmed at 37.0°C and 5.0% CO 7. If you are not planning to humidify the incubator, leave the humidity tray in place and skip to Step 4 in Section 3.3 below. M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 21: Using The Humidity Tray

    Table 4: Temperature & CO Setpoint Ranges Parameter Available Setpoint Range Temperature 10ºC to 50ºC (must be at least 1ºC above ambient) 0.2% to 20% To set the Temperature: ∗ 1. Press the Programming ( ) key. New Brunswick Scientific User’s Guide...

  • Page 22: Referencing Co 2 With Auto Zero

    “click” when the programmed level is entered: this is the CO valve opening. NOTE: There is a magnetic switch above the outer door, and a magnet mounted in the door that switches off the CO valve when the outer door is opened. M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 23: Programming The Alarm System

    3.6.2 Setting the CO High & Low Alarms 1. While you are still in Alarm Program Mode (see Step 1 of Section 3.6.1 ▲ above), press the key. The display will show CO New Brunswick Scientific User’s Guide...

  • Page 24: Door Open Alarm

    This helps prevent unnecessary alarms from occurring. To change the delay: ▲ 1. In Alarm Program Mode (see step 1 of Section 3.6.1 above), press the key. The display will show dr . dEL (across both windows). M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 25: Chamber Alarm System

    You can cancel this alarm message by pressing any key. The duration of the audible alarm can be adjusted from inactive to continuous (see Section 3.6.4 above). New Brunswick Scientific User’s Guide...
  • Page 26 CANCEL ALARM BY ALARMS ALARM - MESSAGE ON PRESSING ANY KEY CANCELLED SCREEN CANCEL HAVE CONDITIONS VISUAL AND AUDIBLE MESSAGE BY RECOVERED TO ALARMS CANCELLED, PRESSING ANY WITHIN +/- 0.1? MESSAGE LEFT ON SCREEN M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 27: Temperature Sensor System Alarms

    If the problem persists, the alarm will recur; if this happens, please contact your service representative or your distributor immediately. New Brunswick Scientific User’s Guide...

  • Page 28: Co 2 Control System Alarm

    If this is the case, at the end of the AutoZero procedure, the following message will appear: AUTOZERO FAILED. This means that the CO Sensor is defective and requires replacement. If this alarm occurs, contact your service representative immediately. M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 29: Routine Maintenance

    CO to 0.2% to prevent CO from being injected into the chamber and giving a false reading. A flow rate ≤ 0.5 liters/minute is used to take a sample. New Brunswick Scientific User’s Guide...
  • Page 30 We recommend that you perform a CO AutoZero prior to sampling (see Section 3.5). 3. We also recommend that you AutoZero the CO system once a month to ensure that the CO level is correct. M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 31: Cleaning & Disinfecting

    It is very important to ensure that no liquid is spilled onto the white porous CO sensor cover at the rear of the chamber. Failure to use the protective cover could result in damage to the sensor and may affect your warranty. New Brunswick Scientific User’s Guide...
  • Page 32 12. When the incubator has stabilized, carry out an AutoZero and re-program the desired CO level. It may be necessary to open the glass door briefly if, after performing an AutoZero, the CO level is too high. M1310-0050 Model CO-150 CO Incubator User’s Guide...

  • Page 33: Specifications

    Model CO-150 Incubator Specifications Digital programming via microprocessor control on 0.1°C increments. Measurement of chamber, outer door & both heating element temperatures via 4 RT matched Temperature Management thermistors (sensitivity 0.01°C) “Out of Limits” temperature protection system independent of microprocessor control.
  • Page 34 Model CO-150 Incubator Specifications Grounded Electrical Supply 110-120V 50/60Hz Voltage: 220-240V 50/60Hz Power: 500 watts Energy to maintain 37°C: < 0.1kwh 10 - 50°C Storage Temperature NOTE: Factory calibration of the incubator is carried out at 37ºC, 5.0% CO 95% RH, in an ambient temperature of 20-25ºC with no heat-generating apparatus inside the chamber.

  • Page 35: Options & Accessories

    CO2 Gas Analyzer Kit P0628-5040 10 Spare CO2 Gas Analyzer Tubes P0628-5050 Stacking Stand For 2 Units P0628-5220 Below Bench Stand P0628-5230 Extra Shelf, Non-perforated P0628-5190 Extra Shelf, Perforated P0628-5200 Multi-Position Wire Shelf Racks P0628-5210 New Brunswick Scientific User’s Guide...
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  • Page 37: Drawings & Tables

    Control Panel Rear Panel Chamber Alarm System Flow Chart 8.2 List of Tables Following is a key to the tables in this manual: Table Description Page Accessories Provided Utilities Function Keys Temperature & CO Setpoint Ranges New Brunswick Scientific User’s Guide...
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  • Page 39: Symbol For

    Running the, 22 List of, 3 AutoZero Failed Message, 28 Introduction, 13 CAUTION Keypad, 19 Symbol for, 7 Chamber Alarm System Functions of, 25 Chamber Alarms Leveling the Incubator, 17 Flow Chart, 26 Location, 16 New Brunswick Scientific User’s Guide...
  • Page 40 Temperature Sensor System Alarms, 27 Power Cord, 16 Temperature Setpoint Range, 21 Power Switch Lack of, 20 PVC Tubing, 16 Utilities, 16 Recalibration, 34 WARNING Symbol for, 7 Warranty, 9 Sensor Covers, 16, 20, 31 M1310-0050 Model CO-150 CO Incubator User’s Guide...

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