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5.11 Illumination
The illumination has the following specifications:
•
LED
: 3W NeoLED
•
Power supply
: Primary 100–240 VAC, 50/60 Hz
5.12 Auto-Off Function
After 20 to 30 minutes of non-use, the light source will turn off automatically.
The indicator LED (1) will flash once every 3 seconds. To turn the light back on,
press function button (2). To turn off the auto-off function, press the button
(2) for 3 seconds. This will cause the red indicator LED (1) to turn off and the
microscope light to stay on. Press the button for another 3 seconds, it will
make indicator LED (1) flash and the auto-off function is back on.
6.0 Phase contrast (model 78904-24)
6.1 Use of phase contrast
The phase contrast method was
designed in 1934 by the Dutchman
Frits Zernike to observe very thin or
transparent objects. This technique
uses the fact that light travelling
through tissue undergoes a phase
shift due to diffraction.
By recombining the phase shifted
light with the background light, a
contrasted image appears in the
eyepiece.
6.2 Using the Phase contrast slider
•
Keep the phase contrast slider face up (text up); insert it from left to right into the condenser slider socket as the
direction of the arrow pointed
•
Each slider has 3 positions, 2 phase contrast positions and in the center of the slide the brightfield position
for normal use without phase contrast. Each phase contrast objective used has to be matched with the phase
contrast ring on the slider. For example: when the 10x phase contrast objective is used the slider should be
positioned to match the 10 phase diaphragm.
Note: The phase diaphragms in the sliders are pre-centered and do not need to be adjusted in operation
6.3 Using the Zernike phase contrast set
Any MSU-600 model with a Zernike phase contrast set comes with the condenser and objectives already mounted and
centered on your microscope. If you suspect misalignment or want to check the alignment please see the next section
for "6.4 Centering the phase rings". The height of condenser can be adjusted in height by means of a rack and pinion
movement. In this way the light beam is concentrated in the specimen for an optimum resolution.
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