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5.5 Eyepieces
Using a binocular (or trinocular) tube is less tiring for the eyes than the use of a monocular tube. In order to obtain a
smooth "compound" image, one should go through the steps below.
5.5.1. The interpupillary distance
The correct interpupillary distance is reached when one round image is seen in the field of view (see image below).
This distance can be set by either pulling the tubes towards each other or pulling them from each other. This distance
is different for each observer and thus should be set individually. When more users are working with the microscope
it is recommended to remember your interpupillary distance for a quick set-up during new microscopy sessions. The
MSU-600's swiveling eyepiece tube can be rotated 360º. You can select corresponding eye point height according to
your own preference.
5.5.2. The correct eye point
The eye point is the distance from the eyepiece to the user's pupil. To obtain the correct eye point, move the eyes
towards the eyepieces until a sharp image is reached at a full field of view.
5.5.3. Adjusting the diopter(s)
In order to compensate for human eye differences, distortion, thickness differences in cover glasses and tune for the
best parfocality between objectives, one can use the diopter to do so. Take a good prepared slide for your reference:
5.5.3.1 Microscope models equipped with one diopter adjustment
•
Position the diopter marking on the zero point
•
Look into both eyepieces and focus on the specimen
•
Close the right eye and look into the eyepiece with diopter adjustment, rotate the diopter adjustment from "+"
to "–" until the selected area get as sharp as possible
5.5. 3.2 Microscope models equipped with two diopter adjustments
•
Set (both) the diopter adjustments of the eyepieces to "0"
•
Select the 10x objective, look for a interesting area on the specimen and focus on this area
•
Select the 40x objective and focus on the specimen
Warning: don't change the coarse and fine adjustment any more.
•
With your dominant eye open (close your other eye), rotate the diopter adjustment from "+" to "–" until the
selected area get as sharp as possible as with the 40x objective
•
If during this operation the image becomes unsharp, take your eyes from the eyepieces and turn the diopter
adjustment, without looking into the eyepieces, a few divisions back from "-" to "+" .
•
Look into the eyepieces again and turn the diopter adjustment from "+" to "-" until the selected area on your
specimen gets the optimal sharpness
•
Repeat for your non-dominant eye, and with the second diopter
Verification:
•
Take your eyes from the eyepieces and look for 2 seconds to a far point in the room in order to "reset" your eyes
•
Look again into the eyepieces. If the adjustment is not good, repeat the operation until you reach the same
sharpness for the 10x and 40x objective without touching the coarse and micrometric adjustments
Field of view
before adjustment
Field of view
after adjustment
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