Related Manuals for Cole Parmer MSU-400 Series
Summary of Contents for Cole Parmer MSU-400 Series
- Page 1 Installation and Operating Instructions MSU-400 Series Compound Microscopes Models 78904-02, -04, -07, -10, -13 78904-02 78904-07 78904-13...
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Page 2: Table Of Contents
Notes _________________________________________________________________________ 19 Introduction Thank you for purchasing a Cole-Parmer MSU-400 Series compound microscope. The MSU-400 series has been designed with all kinds of Educational and Life Sciences applications and great durability in mind. This resulted in a modern, robust and high-level microscope for advanced use, equipped with the best optical and mechanical components. -
Page 3: General Safety Instructions
General safety instructions Intended use: A non-medical device This microscope is intended for general observation of cells and tissues, with transmitted/reflected illumination and with the specimen fixed on a slide. Dangers associated with the operation • Improper use could result in injury, malfunction or damage to property. It must be ensured that the operator informs every user of existing hazards •... -
Page 4: Disinfection And Decontamination
Dirt or fingerprints will reduce the life span and can result in uneven illumination, lowering the optical performance • Use only original MSU-400 Series replacement LEDs • The use of other products may cause malfunctions and will void warranty •... -
Page 5: Environment, Storage And Use
Environment, storage and use • Maximum altitude: 2000 m • Pollution degree: 2 • This product is a precision instrument and it should be used in a proper environment for optimal use • Install your product indoors on a stable, vibration free and level surface in order to prevent this instrument to fall thereby harming the operator •... -
Page 6: Models
Models The Cole-Parmer MSU-400 Series compound microscopes are equipped with HWF 10x/20 mm eyepieces, and are available in the following brightfield and phase contrast models. Model 78904-13 includes a fluorescence attachment with four 5 W LEDs for fluorescence excitation from 450 to 470 nm (blue), 515 to 535 nm (green), 390 to 400 (violet) and 360 to 370 nm (ultraviolet;... -
Page 7: Components Of The Microscope
Components of the microscope The names of the several component parts are listed below and are indicated in the picture: Camera focus adjustment ring Diopter adjustment Trinocular tube Slide for polarization filter Microscope head Nosepiece Transport handle Objectives Height adjustment condenser Stage with X-Y mechanical stage Slide protection handle X-Y stage controls... -
Page 8: Preparing The Microscope For Use
The microscope head The standard MSU-400 series configuration is supplied with the head assembled. However, if your order contains a fluorescence attachment (model 78904-13) then this should be mounted first; see pages 14-16 for supplementary manual. -
Page 9: Operation
The eyeshades (optional) The eyepieces can be equipped with optional rubber eyeshades. This prevents damage to the lens, and prevents stray light. The eyeshade can simply be slipped over the eyepiece Connecting the power cord The MSU-400 microscopes support a wide range of operating voltages: from 100 to 240 VAC. Please use a grounded power connection •... -
Page 10: Focusing And Slide Protection
Focusing and slide protection Select the 4x objective and make sure that it is placed correctly in the optical path Move the stage to the top by using the coarse adjustment knob and focus till the image appears Rotate the fine focusing knob to sharpen the image When you perform focusing with a S100x objective, you need to lock the slide protection handle. -
Page 11: Abbe Condenser
Abbe condenser Beneath the object stage an Abbe condenser N.A. 1.25 is mounted. The condenser can be adjusted in height by moving the rack and pinion knob beneath the mechanical stage. By adjusting the condenser you can focus the light on the specimen for a optimized contrast. -
Page 12: Use Of The S100X Oil-Immersion Objective
Use of the S100x oil-immersion objective The MSU-400 Series microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please follow the below instructions on how to use this objective: Remove the dust protection cap from the revolving nosepiece to mount the S100x objective... - Page 13 Centering the phase rings The Zernike phase disc has five positions: 0 for brightfield observation (this position also has an iris diaphragm), 10, 20, 40, and 100. These positions correspond to the respective phase contrast objectives 10x, 20x, 40x and 100x. When the condenser is in the “0”...
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Page 14: Supplementary Manual For Fluorescence Attachment (Model 78904-13)
Supplementary manual for fluorescence attachment (model 78904-13) Installation of the fluorescence illuminator and microscope head Most parts are already mounted on the microscope. Only two parts are packed separately. The microscope head (B) and the fluorescence illuminator (F). Eyepieces Protection screen Microscope head Fluorescence illuminator Fluorescence attachment... - Page 15 Operation of reflected illumination system (LED fluorescence unit) To get the best result when observing with fluorescence please pay attention to the following: Select the fluorescence filter that matches the fluorochrome and fluorescent characteristic No autofluorescence specimen or device should be in the optical system, such as the cedar immersion liquid which has cyan autofluorescence Always turn off the transmitted illumination before turning on the reflected illumination system Set and adjust the fluorescence unit...
- Page 16 Operation of the field diaphragm The field diaphragm has been pre-centered and is ready to use, but due to possible vibration or inclination during transport it may deviate, therefore always double check. If it is not pre-centered, then please follow the following steps: Use objective 10x Push in the field diaphragm switch (the one in the front, (fig.2, A), a light spot will be visible in the field of view Adjust the centering if the light spot deviates from the center of the view of view (fig.
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Page 17: Maintenance And Cleaning
Maintenance and cleaning Always place the dust cover over your microscope after use. Always keep the eyepiece and objectives mounted on the microscope to avoid dust entering the instrument Cleaning the optics When the eyepiece lens or front lens of the 10x or S40x objective are dirty, they can be cleaned by wiping a piece of lens paper over the surface (circular movements). -
Page 18: Digital Cameras
Digital cameras For trinocular models, slide the camera with mounted C-mount adapter into the 23,2mm tube of the photo port. Take an easy-to-view specimen and focus the image through the microscope’s eyepieces. For focusing the camera, slowly move tube (A) up and down while watching at the screen untill the camera view is in focus Follow the manual that comes with the camera for camera operation Accessories and spare parts... -
Page 19: Notes
Notes ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________ ___________________________________________________________________________________________________... - Page 20 Toll-free: 1-800-323-4340 Phone: 1-847-549-7600 Fax: 1-847-247-2929 coleparmer.com ©2022 Cole-Parmer Instrument Company, LLC. 1065Y62_M_CP_MSU-400_Series_Compound Microscopes_Manual...
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