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Berthold Crocodile miniWorkstation Operating Manual

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LB 925
Crocodile miniWorkstation
Operating Manual
84024BA2
Rev. No.: 03, 04/2020

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Summary of Contents for Berthold Crocodile miniWorkstation

  • Page 1 LB 925 Crocodile miniWorkstation Operating Manual 84024BA2 Rev. No.: 03, 04/2020...
  • Page 3 The information in this guide is subject to change without notice. DISCLAIMER TO THE EXTENT ALLOWED BY LAW, BERTHOLD TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

  • Page 4: Table Of Contents

    1. Preface LB 925 Crocodile miniWorkstation Table of Content Preface ....................... 7 Contact information ................7 Symbols on the instrument ..............8 The Operating Manual ................8 1.4.1 Validity of the operation manual ............8 1.4.2 Copyright ....................9 1.4.3 Warnings, notes and symbols used in this manual ......
  • Page 5 LB 925 Crocodile miniWorkstation 1. Preface Setup an Assay ..................34 5.4.1 Edit an incubation step ............... 35 5.4.2 Edit a shaking step ................36 5.4.3 Edit a washing step ................36 5.4.4 Edit a Dispense Step ................38 5.4.5 Edit Measure (5-in-one only) ..............
  • Page 6 11. Warranty and Technical Issues ..............65 11.1 Special spare parts ................65 11.2 Warranty statement ................65 11.3 Customer service ................... 66 11.3.1 Return Shipment to Berthold Technologies ........66 12. Index ......................68 Page 6 of 68 84024BA2 Rev. 03, 04/2020...

  • Page 7: Preface

    LB 925 Crocodile miniWorkstation 1. Preface Preface Contact information BERTHOLD TECHNOLOGIES GmbH & Co.KG Calmbacher Straße 22 D-75323 Bad Wildbad Germany Sales and Customer Support Internet: www.berthold.com/en/bioanalytic/ Phone: +49 (0) 7081-177-0 Fax: +49 (0) 7081-177-100 E-Mail: bio@berthold.com Central Customer Service...

  • Page 8: Symbols On The Instrument

    1.4.1 Validity of the operation manual The operating manual is valid from the delivery of the Berthold product to the user until its disposal. Version and release date of this operating manual can be found in the bottom of each page.

  • Page 9: Copyright

    LB 925 Crocodile miniWorkstation 1. Preface The manufacturer reserves the right to make changes to this operating manual at any time without stating reasons. However, should questions arise which are not answered in this manual please contact BERTHOLD, bio@berthold.com. Revision History of the manual...

  • Page 10: Intended Use

    Table 1-2: Typographical Conventions used in the manual Intended Use The Crocodile miniWorkstation has been specifically designed to automate assay applications in 96 well format. The instrument is a multi-function device that performs single function- or combined function protocols which have been designed and validated by the user.
  • Page 11 LB 925 Crocodile miniWorkstation 1. Preface Figure 1-1Crocodile miniWorkstation The Crocodile miniWorkstation combined with Crocodile Control Software may be used for the following purposes:  Veterinary medicine, e.g. immunoassays, microbiology, clinical chemistry.  Food industry, e.g. development and quality control in production.

  • Page 12: Important Information

     Only accessories, in particular desktop power supply, power supply cable and data connection cable if any, supplied with the instrument or by Berthold Technologies for working with this instrument may be used for operation.  Never connect the power cord near liquids to avoid electric shock or fire.
  • Page 13 LB 925 Crocodile miniWorkstation 2. Important information  Berthold Technologies assumes no liability for any damages, including damages to third parties, caused by improper installation, use or handling of the equipment. The instruments are live and improper handling can lead to damage.
  • Page 14 Berthold Technologies. Before further use, the instrument must be reassembled and inspected in accordance with the instructions in the service manual.  Only parts specified by Berthold Technologies may be used for maintenance work.  Switch off the instrument before disconnecting the plug.

  • Page 15: Consignes De Sécurité

    échéant, fournis avec l'instrument ou par Berthold Technologies pour travailler avec cet instrument peuvent être utilisés pour le fonctionnement.  Ne connectez jamais le cable d'alimentation à proximité de liquides afin d'éviter tout risque d'électrocution ou d'incendie.
  • Page 16 2. Important information LB 925 Crocodile miniWorkstation  Berthold Technologies n'assume aucune responsabilité pour tout dommage, y compris à des tiers, résultant d'une installation, d'une utilisation d'une manipulation inappropriées de l'équipement. Les appareils sont sous tension et une mauvaise manipulation peut causer des dommages.
  • Page 17 être effectués que par des techniciens de service qui ont été formés et autorisés par Berthold Technologies. Avant toute utilisation ultérieure, l'instrument doit être réassemblé et testé conformément aux instructions du manuel de service.

  • Page 18: Sicherheitshinweise

     Zum Betrieb darf nur Zubehörteile, insbesondere Tischnetzteil, ggf. Netzversorgungskabel Datenverbindungskabel, verwendet werden, die mit dem Instrument oder von Berthold Technologies für die Arbeit mit diesem Instrument geliefert wurde.  Schließen Sie das Netzkabel niemals in der Nähe von Flüssigkeiten an, um einen elektrischen Schlag und Brand zu vermeiden.
  • Page 19 2. Important information  Alle gelieferten Instrumente und alle Zusatzgeräte müssen geerdet werden. Verwenden Sie dreipolige, geerdete Stecker.  Berthold Technologies übernimmt keinerlei Verantwortung für Schäden, einschließlich Schäden gegenüber Dritten, die durch unsachgemäße Installation, Gebrauch oder Handhabung der Geräte hervorgerufen werden. Die Geräte sind stromführend und unsachgemäße Handhabung kann zu Schäden führen.
  • Page 20 Technologies trainiert und autorisiert wurden. Vor der weiteren Verwendung muss das Instrument wieder zusammengebaut und gemäß der Anweisungen im Servicemanual geprüft werden.  Für Wartungsarbeiten dürfen nur die von Berthold Technologies spezifizierten Teile verwendet werden.  Schalten Sie das Instrument aus bevor Sie den Stecker abziehen.

  • Page 21: Further Instructions

    To ensure proper operation of the system, it is recommended to operate Qualification Procedures at defined time intervals and to use high quality equipment. Berthold Technologies offers Validation Tools for the Crocodile miniWorkstation, including the IQ/OQ/PQ Qualification Package and the Absorbance TestPlate.
  • Page 22 2. Important information LB 925 Crocodile miniWorkstation A failure in the performance of Quality Control checks could result in erroneous test data. Disposal Decontaminate the instrument before disposal! The Crocodile contains electronic parts. To prevent environmental pollution please dispose of the instrument and the corresponding accessories according to local legislation.

  • Page 23: Installation

    3. Installation Installation Unpacking and Check of Shipment The Crocodile miniWorkstation and the acrylic cover are shipped in a cardboard box containing foamed inserts to protect the instrument and the cover from damage. Also included is an accessory box. Carefully unpack the instrument and the accessories.

  • Page 24: Installation Of The Acrylic Cover

    3. Installation LB 925 Crocodile miniWorkstation Figure 3-1: Mounting position of the transportation lock screw Installation of the Acrylic Cover The acrylic cover is shipped as a single component and must be installed prior to use. Install the acrylic cover as shown in the drawing below using the two cover-pins and fix them with two screws each at the right and left side of the cover.

  • Page 25: Connecting Cables

    USB connection 4 unused ports (do not connect) Figure 3-3: Crocodile miniWorkstation rear view  For connecting the Crocodile miniWorkstation to PC, use the supplied USB cable and read additionally chapter 5.1 Software Installation. Operating conditions NOTICE Operation Temperature: 10-35°C up to 75% humidity (non- condensing).

  • Page 26: Hardware Description

    4. Hardware Description LB 925 Crocodile miniWorkstation Hardware Description The Crocodile miniWorkstation is a compact desktop unit which has been specifically designed to automate assay applications in 96 well formats. The Crocodile 5-in-one is equipped with all necessary components required for running an ELISA Assay (washer, dispenser, shaker, incubator and reader).

  • Page 27: Internal Waste Container

    LB 925 Crocodile miniWorkstation 4. Hardware Description Figure 4-2: Microplate holder Internal Waste Container The Crocodile miniWorkstation is equipped with a built-in waste container for priming and washing the dispenser and washer lines. Waste container Figure 4-3: Waste container and dispenser unit...

  • Page 28: Dispenser

    4. Hardware Description LB 925 Crocodile miniWorkstation Dispenser The Crocodile includes four integrated dispensers, each equipped with an independent pump. Figure 4-4: Position of the 4 dispenser inlets and 1 waste outlet at the instrument’s side. The dispenser pumps are numbered to correspond with the dispensers at the detection optics.

  • Page 29: Shaking Function

    If a connection is leaking, it has to be replaced. See chapter 7.5 for instructions. Shaking Function The Crocodile miniWorkstation features a shaking mode with independent linear motion from 5 – 20 Hz. The function is controlled by software.

  • Page 30: Reader (5-In-One Only)

    4. Hardware Description LB 925 Crocodile miniWorkstation Dispensing needles Aspiration needles Figure 4-7: Dispensing and aspiration needles at the washer manifold Reader (5-in-one only) The reader has a dynamic range between 0-3.0 OD. The spectral range is from 400 – 690 nm. Pre-installed filters for mono and bi- chromatic reading are: 405;...

  • Page 31: Software

    The description of the MikroWin module is not part of this manual. Software Installation and connection NOTICE The Crocodile miniWorkstation is operated via Crocodile Control Software, delivered on a data carrier. Install the Software according to the instructions before connecting the instrument to PC.
  • Page 32 Password request: Request the password via E-Mail, Fax or Web. Press the respective E-Mail, Web or Fax button, insert the required information in the predefined mail, fax form or web registration form and forward it to BERTHOLD TECHNOLOGIES. Alternatively request the password via the BERTHOLD TECHNOLOGIES homepage.

  • Page 33: Commissioning And Installation Check

    LB 925 Crocodile miniWorkstation 5. Software If MikroWin software has been ordered: The MikroWin software is protected by a USB dongle. Install the software before connecting the USB dongle to the computer. The installation will copy the necessary USB drivers required during USB device detection.

  • Page 34: Setup An Assay

    5. Software LB 925 Crocodile miniWorkstation [Instrument]: allows changing the plate carrier position [Move to insert position] and the [Incubator settings…] independently from a protocol. [Maintenance]: contains maintenance menus [Dispenser…] and the [Washer…] and information about the [Instrument configuration…]. [Select Assay]:...

  • Page 35: Edit An Incubation Step

    LB 925 Crocodile miniWorkstation 5. Software Configure the Assay Steps: Every step in the [List of Selected Steps] must be configured using <Edit Name> and <Edit step>. Click on a step and on the respective button afterwards to rename the step or to open a submenu for editing. For details about editing steps, see chapter 5.4.1 - 5.4.6.

  • Page 36: Edit A Shaking Step

    5. Software LB 925 Crocodile miniWorkstation 5.4.2 Edit a shaking step Insert your settings for [Duration], [Amplitude] and [Speed] into the Shaker dialog box. For shaking inside the incubator tick the respective check box. Confirm your entries with <OK>. 5.4.3 Edit a washing step Washing is one of the most important steps in colorimetric ELISAs.
  • Page 37 LB 925 Crocodile miniWorkstation 5. Software Select a [Wash Process] and the number of [Cycles] for this process. The list contains self-declaring processes for priming, dispensing, aspiration or clearance of the system. Additionally, three wash processes combining Aspiration (Asp) and Dispense (Disp) are available.

  • Page 38: Edit A Dispense Step

    5. Software LB 925 Crocodile miniWorkstation 5.4.4 Edit a Dispense Step Four dispensers are available. Before use of a dispenser, it has to be primed. Select a [Reagent] inlet and set the [Volume] For priming, tick the check box [Prime].

  • Page 39: Edit Measure (5-In-One Only)

    LB 925 Crocodile miniWorkstation 5. Software 5.4.5 Edit Measure (5-in-one only) The Measure step must always be defined, because no filter is pre- set. Choose between [Single Wavelength] and [Reference Measurement] (two measurements) in the [Method] drop- down list box.

  • Page 40: Run An Assay

    5. Software LB 925 Crocodile miniWorkstation The Crocodile Control Software allows manual steps of an individual [Duration] and a selected [Plate Position]. Select [Auto Continue] or [User Continue], set the [Duration] time and select the [Plate Position]. [Auto Continue] After a certain period, which is selected in the Duration drop-down box, the next assay step will be executed.
  • Page 41 LB 925 Crocodile miniWorkstation 5. Software Enter the [Plate ID] and, optional, information about the assay and the reagents to be used and confirm with <OK> Buffers and reagents defined in the <Washer Dispenser Inlet Label> of the Assay will occur as [Meta Data] on the left side.

  • Page 42: Measurement Units (5-In-One Only)

    5. Software LB 925 Crocodile miniWorkstation Measurement Units (5-in-one only) The Crocodile miniWorkstation with Crocodile Control Software obtains O.D. values as measurements results. This does not directly represent the analytical result of an analysis. It is subject to further data evaluation.

  • Page 43: Performance And Quality Control

    LB 925 Crocodile miniWorkstation 6. Performance and Quality Control Performance and Quality Control Performance Checks Performance checks are necessary for every part of the miniWorkstation and particular instrument functions in defined time intervals. Please view the chapter on Software before programming the respective protocols with the Crocodile Control Software.

  • Page 44: Dispenser

    6. Performance and Quality Control LB 925 Crocodile miniWorkstation 6.1.2 Dispenser Check every dispenser separately. Dispense 3 rows/ dispenser as a minimum. Weigh an empty 96 well microplate and note the weight. Program a protocol for a dispenser, that primes first with 2000µl and dispenses 100µl of distilled water to each selected...

  • Page 45: Quality Control Measurements

    Procedures in defined time intervals and use high quality equipment. A failure in the performance of quality control checks could indicate a risk of error in test data. Berthold Technologies offers Validation Tools for the Crocodile miniWorkstation, including the IQ/OQ/PQ Qualification Package and the Absorbance TestPlate.

  • Page 46: Cleaning And Maintenance

     In case of questionable measurement results, please follow the steps listed below to ensure the instrument is functioning properly.  Before service or shipping any instrument back to Berthold Technologies, the instrument must be decontaminated. See the information on return shipment and the Confirmation on Decontamination in chapter 11.3.1 for further instructions.

  • Page 47: Cleaning

    LB 925 Crocodile miniWorkstation 7. Cleaning and Maintenance Recommended Maintenance Schedule: Daily/ Before Weekly/ Yearly/As before/ Storage/ As needed needed after use Shipment   Clean external surface   Clean microplate holder Clean/Empty Dispenser   System Clean/Empty Washer ...

  • Page 48: Cleaning Of Surfaces

    7. Cleaning and Maintenance LB 925 Crocodile miniWorkstation  Metal parts: stainless steel, nickel-plated  Washer system: Hydex 202 Polyurethane rigid, Stainless steel, PP, Peek, rubber-FFPM, PVDF, HNBR, Hostaform, EPDM, Neoprene, PVC  Dispenser system: PTFE (Teflon), Kel-F, PVC, Peek, EPDM 7.2.2...
  • Page 49 LB 925 Crocodile miniWorkstation 7. Cleaning and Maintenance Before and after long breaks:  Use at least the following volumes for liquids others than distilled water:  for every dispenser: 2ml per sequence step  for the washer-dispenser unit: 8-10 cycles of 1000µl up to 80ml in total (10ml per needle) per sequence step.
  • Page 50 7. Cleaning and Maintenance LB 925 Crocodile miniWorkstation Before and after long breaks: Use one of the following procedures  Cleaning with 0.5% bleach: 1. Distilled water 2. 0.5% bleach 3. Distilled water (increased number of columns) 4. empty and dry the lines by pumping air ...

  • Page 51: Cleaning The Washer Manifold

    LB 925 Crocodile miniWorkstation 7. Cleaning and Maintenance 7.2.4 Cleaning the Washer Manifold IMPORTANT If the washer needles are clogged, use the provided cleaning wires to dislodge the clogging. Cleaning wires of two different sizes are provided. Use the smaller cleaning wire for the aspiration needles and the bigger one for the dispensing needles.

  • Page 52: Cleaning The Internal Waste Container

    7. Cleaning and Maintenance LB 925 Crocodile miniWorkstation Fitting screw with line Dispenser tips Figure 7-2: Dismounting of dispenser lines and tips Soak the dispenser tips with 0.5% bleach and distilled water. Use the cleaning wires to dislodge the clogging.
  • Page 53 LB 925 Crocodile miniWorkstation 7. Cleaning and Maintenance the reagents in the lines for approximately 10 minutes. Empty and dry the lines, then. Wipe the outside of all lines with 0.5% bleach multiple times and with distilled water afterwards. Follow the description for cleaning the internal waste container and the extraction hose but wipe out with 0.5%...

  • Page 54: Maintenance

    7. Cleaning and Maintenance LB 925 Crocodile miniWorkstation Maintenance 7.5.1 Changing the Dispenser Lines IMPORTANT The user may exchange the dispenser lines if a tubing connection is faulty or if desired, using the Dispenser Tubing Set coming with the instrument. Refer to the Maintenance Schedule in chapter 7.1.
  • Page 55 LB 925 Crocodile miniWorkstation 7. Cleaning and Maintenance Unscrew the tubing’s fitting screw with the respective number at the dispenser. Figure 7-5: Fitting screw at the dispenser A Dispenser Tubing Set comes with the instrument and provides tubing for the dispenser lines. Turn the fitting screw of the new line into the thread at the dispenser and finger- tighten it.

  • Page 56: Troubleshooting

    8. Troubleshooting LB 925 Crocodile miniWorkstation Troubleshooting In cases of malfunction or unexpected measurement values, see the troubleshooting list and/or contact your local distributor or Berthold Technologies. IMPORTANT The list contains troubleshooting items for Crocodile 5-in-one and 4- in-one (without reader).
  • Page 57 LB 925 Crocodile miniWorkstation 8. Troubleshooting from 10-30 minutes.  Test conjugate and substrate for activity. Substrate or conjugate is no longer active or is weak  Sodium azide will inhibit peroxidase reactions. Enzyme inhibitor present  Verify the wavelength and filter settings in the plate Plate reader settings...
  • Page 58 8. Troubleshooting LB 925 Crocodile miniWorkstation High background  Detection antibody is cross-reacting with coating Cross-Reactivity antibody. Run appropriate controls.  Check the water quality. If it is questionable, try an Poor quality water was used to wash the plates...
  • Page 59 LB 925 Crocodile miniWorkstation 8. Troubleshooting Uneven colour development  Ensure that all wells are filled with wash buffer and Incomplete washing of wells are aspirated completely.  Check the washer dispenser functionality: flush the wash head, clean the dispense needles with the cleaning wire if needed.
  • Page 60  Bring reagents and microplate to room temperature before use. Disruption of processing  Repeat process. Automatic sequence  Contact your local distributor or Berthold control disrupted by outer radiation or Technologies for help. electrical burst Table 8-1: Troubleshooting list Page 60 of 68 84024BA2 Rev.

  • Page 61: Technical Data

    LB 925 Crocodile miniWorkstation 9. Technical Data Technical Data Crocodile miniWorkstation: 96-well microplate in solid or strip Sample Format format (ANSI/SLAS standard) Dimensions 26,3 x 62,6 x 25,8 cm; 10.4 x 24.6 x 10.2 inch (W x D x H) Weight 14,8 kg;...
  • Page 62 9. Technical Data LB 925 Crocodile miniWorkstation Reader 0 – 3.0 OD at 450 nm; 0 - 2.5 OD at 405 Dynamic range 400 – 690 nm Spectral range (pre-installed filter: 405, 450, 492, 620 nm) Filter slots Reading channels 8 plus reference channel <1.5% CV (0.01 –...

  • Page 63: External Power Supply

    LB 925 Crocodile miniWorkstation 9. Technical Data Input connector for users’ external Waste container bottle, any size Liquid container: up to 3 different wash Liquid container solutions PC Software Platform/Required Windows® compatible computer, Hardware Pentium-like processor, 1 GB RAM or...

  • Page 64: Preparing Crocodile For Transport

    10. Preparing Crocodile for Transport LB 925 Crocodile miniWorkstation Preparing Crocodile for Transport If it should become necessary that Crocodile has to be serviced please observe the following instructions for shipping it to your local distributor or Berthold Technologies: 1. Clean...

  • Page 65: Warranty And Technical Issues

    11.2 Warranty statement The instrument is sold in accordance with the general conditions of sale of Berthold Technologies GmbH & Co KG and its affiliates and representatives. Berthold Technologies warrants this product to be free of defects in material and workmanship for a period of 12 months from the date of delivery, ex works Bad Wildbad.

  • Page 66: Customer Service

    Berthold Technologies representative. For further problems requiring hardware or software expertise, contact the Technical Support group at Berthold Technologies GmbH & Co KG Here are the contact data: Berthold Technologies GmbH & Co KG Technical Support Calmbacher Str.
  • Page 67 LB 925 Crocodile miniWorkstation 11. Warranty and Technical Issues Confirmation on Decontamination If you return an instrument to Berthold Technologies for servicing purposes which is not properly decontaminated, there will be a health risk for Berthold Technologies employees. We therefore need your confirmation that the instrument was decontaminated and cleaned properly before shipping.

  • Page 68: Index

    12. Index LB 925 Crocodile miniWorkstation Index Acrylic cover ........24 Preparing for transport ....64 Check shipment ....... 23 Quality control......21, 45 Cleaning ........... 47 Reader ..........30 Cleaning of surfaces......48 Return shipment ......66 Cleaning washer needles ....51 RMA number ........

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