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Berthold NightOWL II LB 983 Service Manual

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Service Manual
NightOWL II
LB 983
ID-No. 40508SA2
Rev. 03
17.09.2010

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Summary of Contents for Berthold NightOWL II LB 983

  • Page 1 Service Manual NightOWL II LB 983 ID-No. 40508SA2 Rev. 03 17.09.2010...

  • Page 3: Table Of Contents

    Contents NightOWL LB 983 Contents INSTALLATION Camera installation (Ethernet version) Camera installation (USB version) Connecting the instruments Connecting the PC (Ethernet) Connecting the PC (USB) USB Mode programming Software installation (indiGO) Shipping instructions CALIBRATION 2-10 Focus calibration 2-10 Quantum Efficiency 2-12 Flat field calibration 2-12...
  • Page 4 Contents NightOWL LB 983 CLEANING AND MAINTENANCE WORK Cleaning the camera compartment Cleaning the camera Replacing the air filter Replacing the fuse Setting the door sensor Trouble shooting 5.6.1 Camera lift sits on sample carrier 5.6.2 Camera lift sits on bottom of acquisition chamber 5.6.3 Camera lift hits ceiling of acquisition chamber APPENDIX Technical data...

  • Page 5: Installation

    NightOWL LB 983 Installation 1. Installation Each instrument comes with the following accessories: Manual (either in German or English, depending on your order) indiGO software with ”Calibration Data” on a memory stick Power cord (depending on the country) USB or network cable (depending on the camera version) The camera is packed separately.

  • Page 6: Camera Installation (Usb Version)

    Installation NightOWL LB 983 Camera installation (USB version) The NC 100u uses the USB bus to transfer images and camera control. To install the camera just place the camera on the cam- era plate facing the “FRONT” label to the front. Fix the camera with the 2 fastening screws (3).

  • Page 7: Connecting The Pc (Ethernet)

    NightOWL LB 983 Installation supply and turn the instruments on. 1.4 Connecting the PC (Ethernet) The NightOWL camera transfers the image information using the Ethernet bus. If the NightOWL is directly connected to the Ether- net interface card of the PC, a so called “cross-over cable” is re- quired (included with the shipment).

  • Page 8: Connecting The Pc (Usb)

    Installation NightOWL LB 983 quired. To install the network adapter, please refer to the corre- sponding operation manual. For a proper communication with the NightOWL, the IP address of the PC must be set in the range of 192.168.1.xxx (except 192.168.1.117 ->...
  • Page 9 Now the NightOWL is programmed and after power cycling the instrument (switch off, wait 5 se- conds, switch on) the device will be identified as (Berthold Tech- nologies NightOWL). In addition a NightOWL Serial Port is used to...

  • Page 10: Software Installation (Indigo)

    Installation NightOWL LB 983 1.7 Software installation (indiGO) The indiGO software is included on the enclosed USB stick or CD. Keep this media in a secure place. You will need it again for pro- gram updates or new installation. For the operation of the NightSHADE the indiGO software version 2.00 or higher is necessary.
  • Page 11 NightOWL LB 983 Installation Depending on the hardware you use a default se- lection of system filters might be available and can be installed through the setup. If you want to in- stall the default filters tick the box “Install de- fault filters”.

  • Page 12: Shipping Instructions

    Installation NightOWL LB 983 1.8 Shipping instructions The NightOWL II must be shipped with the camera removed and the foam piece laid on the bottom plate fixed with the camera lift as shown on Figure 2: Figure 2 Transport Figure 1 foam piece holder Proceed as follows: ...
  • Page 13 NightOWL LB 983 Installation screws left and right of the camera, unplug the USB cable and Power supply and carefully take out the camera.  Fix the transport holder for the air adapters as shown on Figure 3. Use the grey spacers to avoid touching the lens. ...

  • Page 14: Calibration

    Calibration NightOWL LB 983 2. Calibration 2.1 Focus calibration To run the focus calibration a focus target is required. This sheet can be found at the end of this document. When printing out the focus target make sure, the scale shown on the target is really 20cm as this will be used in the calibration.
  • Page 15 NightOWL LB 983 Calibration To see the live calibration images, launch The OWLview Tool from indiGO and enable the monitor mode by clicking on the icon. Modify the scaling if required to see details. The whole procedure will take 10 to 15 minutes. In case of contrast problems modify the contrast deviation or the illumination intensity.

  • Page 16: Quantum Efficiency

    Calibration NightOWL LB 983 2.2 Quantum Efficiency The quantum efficiency table describes the spectral efficiency of the CCD chip. The table can be accessed by selecting “Quantum Efficiency” from the Calibration section. Depending on the camera used, this table might be different. When using a NC 100 camera the quantum efficiency table should look like as shown in the image.

  • Page 17: Energy Calibration

    NightOWL LB 983 Calibration The calibration procedure starts after clicking on During the flat field calibration the system takes a photo of the white sheet in the positions listed in the table and creates a flat field correction curve. 2.4 Energy Calibration The NightOWL camera is movable to allow large as well as small image sizes without moving the sample.
  • Page 18 The calibration procedure starts after clicking on The Berthold light standards are adjusted and certified to 20nW. During the energy calibration the system takes images of the light standard in the positions listed in the table and creates an energy correction curve.

  • Page 19: Calibration Data

    NightOWL LB 983 Calibration 2.5 Calibration data When all calibrations are done the calibration and configuration data must be backed up to an external storage device (USB Stick). This can be done in the Serial function of the Calibration section. Fill in the instrument ID number, packet number and serial num- ber and click on to copy the calibration and configuration...
  • Page 20 Calibration NightOWL LB 983  owlCtrl.trc – Communication Protocol file. Might be useful to send in case of any instrument/communication prob- lems.  Indigo.net – User data and web update information. Do not modify update information if not instructed since online up- date might not work after modification.

  • Page 21: Ccd Camera

    NightOWL LB 983 CCD Camera 3. CCD Camera 3.1 Dismantling the Camera Positioning the camera at working height  Make sure that no accessories are placed on the base plate to avoid a collision  Move the camera (z-axis) into the low position (step 98000) using the ser- vice software or indiGO.

  • Page 22: Sample Chamber

    Sample Chamber NightOWL LB 983 4. Sample Chamber 4.1 Taking Off/Attaching the Cover The cover has to be taken off to have access to the camera elec- tronics. Generally, two people are needed to take off the cover. Proceed as follows. Taking the ...

  • Page 23: System Electronics

    NightOWL LB 983 Sample Chamber  Insert retention bolts into the catches foreseen for this purpose.  Push down the cover from above until all four bolts lock in- to place with an audible click. 4.2 System Electronics The system electronics includes the power supply and the connec- tion board which connects the system components with each oth- er;...
  • Page 24 Sample Chamber NightOWL LB 983 Connector Function Table 5-1: connection board CAN Output to dark room (focus, ex filter, heater) connectors Connector from CPU USB connector (to hub) n.c. CAN Output (Fluorescence, Z-drive, ex filter) LED 1 (photographic illumination) LED 3 (photographic illumination) Fan on rear panel n.c.

  • Page 25: Service Software

    The service software is available on the Berthold FTP-server. Make sure that the latest version is being used to avoid problems. By running SERVICE.EXE the service software is starting.

  • Page 26: Check Drives

    Sample Chamber NightOWL LB 983 The main menu will appear. The first step to get connected to the NightOWL is to find the used COM-port. Select [Scan ComPorts] in the [Instrument] menu. This will start a scanning run to locate the NightOWL. If the scan was successful a message box will appear.
  • Page 27 NightOWL LB 983 Sample Chamber On the “Drives” tab you can check if the motors for moving the camera (z-drive), the focus motor (focus) or the motors the filter slider and wheel are working correctly. With the upper slider in each box you can select the motor speed. By moving the lower slider and pressing the [Move Z] you can move the camera drive in the defined position.

  • Page 28: Check Halogen Lamp

    Sample Chamber NightOWL LB 983 4.3.3 Check halogen lamp The halogen lamp can be checked using the “Control” tab. In the upper box the lamp intensity can be selected with the slid- er. The checkbox [Lamp cooling fan] enables or disables the cooling fan above the lamp module.
  • Page 29 NightOWL LB 983 Sample Chamber After calling this function the software downloads the current sta- tus from the instrument. If the download was successful the CAN nodes are listed in a box. All installed CAN nodes are labeled with a green hook. To install a new CAN node, just click on [Install] in the corresponding sec- tion of the desired CAN node.

  • Page 30: Firmware Update

    The FlashWizard is a flashing tool which transfers the new firm- ware into the instrument memory and manages the update auto- matically. The FlashWizard can be downloaded from the Berthold FTP- server. Start the FlashWizard by running FLASHWIZ.EXE. A welcome page will appear.
  • Page 31 NightOWL LB 983 Sample Chamber The FlashWizard tries to connect to the instrument. If the communication works, the firmware upload will start automatical- The whole procedure will take approx. 6 Minutes. After the firmware upload and flash the instrument reboots. If the update process is finished the dialog box shows the follow- ing.

  • Page 32: Fluorescence Illumination

    Sample Chamber NightOWL LB 983 4.5 Fluorescence Illumination 4.5.1 General The fluorescence function allows you to excite fluorescence in the Halogen lamp as 400.. 800 nm range. Thus, a large number of colors can be used light source with NightOWL™. Using suitable filter sets, the excitation light is filtered out of the spectrum of the halogen light source.

  • Page 33: Lamp Replacement

    NightOWL LB 983 Sample Chamber The emission filter wheel is placed in front of the lens; it carries the filters for the fluorescence light. The light guide is fixed by a knurled screw, and can be removed when it is not used. If the fluorescence light source is switched off, the light path is blocked by a special barred position using a lid to avoid incoming light.
  • Page 34 Sample Chamber NightOWL LB 983 The whole module is fixed with only 4 screws (2 at the front, 2 at the rear). These screws can be opened using a 4mm Allen key. Before removing the module the 2 red CAN connectors, one below the halogen board and the other at the filter slider motor), as well as the blue connector from the optical sensor must be removed.
  • Page 35 NightOWL LB 983 Sample Chamber procedure is required to ensure proper results. Please see the in- diGo manual for more information. 4-15...

  • Page 37: Cleaning And Maintenance Work

    NightOWL LB 983 Cleaning and Maintenance Work 5. Cleaning and Maintenance Work 5.1 Cleaning the camera compartment The camera compartment should be cleaned using a mild deter- gent and a lint-free tissue or a vacuum cleaner. To this end, the bottom plate can be removed by holding it at both sloping edges on the side.

  • Page 38: Setting The Door Sensor

    Cleaning and Maintenance Work NightOWL LB 983 With the screwdrivers, pull out the fuse inset. Replace the fuses (make sure they match the required line volt- age). Insert the fuse holder again until it clicks into place. Use only fuses that match the value and the line voltage in- dicated on the instrument.

  • Page 39: Camera Lift Sits On Bottom Of Acquisition Chamber

    NightOWL LB 983 Cleaning and Maintenance Work 5.6.2 Camera lift sits on bottom of acquisition chamber Turn the spindle on the camera transport unit to the right so that the lower level of the camera transport unit moves up. This will create so much clearance that the blockage is removed and the camera can be controlled again via the software.

  • Page 41: Appendix

    NightOWL LB 983 Appendix 6. Appendix 6.1 Technical data Camera Peltier/air-cooled CCD slow scan camera C-Mount adapter Image scale and focus can be changed by moving the camera up and down via software. Noise Readout:  8 e - RMS Exposure time from milliseconds to hours Binning of pixels...
  • Page 42 Camera head: 110 mm x 250 mm x 110 mm Sample chamber (inside): 400 mm x 380 mm x 450 mm Weight approx. 85 kg Operating voltage 94,5 to 240 VAC Computer Minimum requirements: PC of industry standard with 1.5 GHz Pentium processor 512 MB RAM Graphics mode, 1208 x 1024, true color 17"...

  • Page 43: Spare Parts List

    NightOWL LB 983 Appendix 6.2 Spare parts list Table 6-1 Spare parts list Description ID No. Power supply 50370 Retractable measuring table 46417 Fluorescence light module 46662 Light bulb for fluorescence light source 50218 LED for photographic illumination 45194 Connection board 45338 CPU with CAN add on 35263...

  • Page 44: Wiring Diagrams

    6.3 Wiring diagrams On the following pages you’ll find the wiring diagrams below: Wiring diagram LB 983 NightOWL II Wiring diagram Connection board...
  • Page 45 NightOWL LB 983 Appendix Circuit diagram connection board...
  • Page 47 NightOWL LB 983 Appendix Calibration Sheet Please make sure that the distance on the calibration sheet is 200mm after printing out.
  • Page 48 Index Air filter 5-1 Illumination 6-1 Automation port 6-1 Lens 6-1 Binning of pixels 6-1 Light source 4-12 Camera 6-1 Noise 6-1 CAN modules 4-8 Computer 6-2 connection board 4-4 Operating voltage 6-2 Dimensions 6-1 service software 4-5 Exposure time 6-1 Temperature 6-1 firmware update 4-10 FlashWizard 4-10...

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