Related Manuals for PERSEE T8DS
Summary of Contents for PERSEE T8DS
- Page 1 T8DS U V - V I S S P E C T R O P H O T O M E T E R U S E R M A N U A L A n a l y t i c a l...
- Page 2 Introduction Thank you for your purchase of the Persee Analytics Inc. T8DS spectrophotometer. UV-Visible Spectrophotometer analysis is a well-accepted, documented technique with many applications. The technique is extensively used for the analysis of agricultural products, public health, environmental protection, life science industries and many other organic and biochemical applications.
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Page 3: Table Of Contents
C o n t e n t s Contents ............................I Chapter 1 Instrument Introduction ..................... 1 1.1 Components inspection ....................1 1.2 UV/Vis spectrophotometer and method ................1 1.2.1 UV/Vis spectrophotometer method ................. 1 1.2.2 UV/Vis spectrophotometer ..................1 1.2.3 Condition of measure method ................ - Page 4 4.12 TR19-1 Test tube cell holder ..................34 Chapter 5 Maintenance ......................35 5.1 Points for attention: ......................35 5.2 Daily care ........................35 5.3 Trouble shooting and maintenance ................36 Appendix 1 Light source replacement ..................40 Replace the tungsten lamp .................... 40 Replace the Deuterium lamp ..................
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Page 5: Chapter 1 Instrument Introduction
Chapter 1 Instrument Introduction 1.1 Components inspection After the instrument has been unpacked, please check carefully all the parts provided against the packing list. In the event of error or damage, contact your local sales agent immediately. A complete set with the instrument according to the packing list should be supplied. -
Page 6: Condition Of Measure Method
1.2.3 Condition of measure method The choice of measuring wavelength Normally taking the wavelength which has the most absorbance as the measuring wavelength ( ) to attain the highest analysis sensitivity is called maximum absorbance principle. The absorbance will change very little during the wavelength’s change around the , giving a good precision. -
Page 7: Components Description
1.3 Components description Figure 1.1 shows the basic T8DS instrument. Figure 1.1 Instrument 1.3.1 Front view The front view is shown in Figure 1.2, the name and function of all the components are described below: Figure 1.2 Front view... -
Page 8: Rear View
Instrument cover Front panel of the sample compartment—standard front panel for the sample compartment. It can be replaced when using some accessories. Chassis—the steel chassis can greatly reduce vibration. Instrument label Sample compartment cover—Open the cover to gain access to the sample compartment. 1.3.2 Rear view The rear view is shown as Figure 1.3, the name and function of all components are described below:... -
Page 9: Side View
1.3.3 Side view The side view is shown as Figure 1.4, the name and function of all components is described below: A Left side view B Right side view Figure 1.4 Side view 1. Power switch 2. Light source cover—Open the cover to gain access to the light source, For replacing the deuterium lamp and tungsten lamp (refer to “Appendix 1 light source replacement”). -
Page 10: Specifications
1.4 Specifications Figure 1-3 Item Specification Wavelength 900nm 190nm range Spectral 5nm 2 nm 1 nm 0.5 nm 0.2 nm 0.1nm ( 6 steps) bandwidth Resolution 0.01nm The start and end wavelength can be set by the span of Setting 1nm. -
Page 11: Chapter 2 Installation
Chapter 2 Installation 2.1 Installation location T8DS double-beam UV/Vis spectrophotometer is a versatile high-precision laboratory instrument. For its optimum performance and its usable longevity, the instrument should be installed in a suitable location with the following requirements: Avoid high temperature and high humidity environment. An environmental temperature should be 15C 35C. - Page 12 Figure 2.1 Connect the instrument’s RS 232 communication port to the PC with RS 232 cable. Connect the printer’s parallel port or USB port to the PC with communication cable, and setup the printer driver in the PC (the detailed information refer to the printer manual) Both ends of all the connecting cables should be securely tightned to the sockets to prevent poor connection.
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Page 13: Start
2.3 Start Make sure there is nothing installed in either of the sample cells which could block the light path. Turn on the power for the printer, then the power for the computer and finally the power for main unit. The computer will start OS automatically. Select UVWin software in the Start menu. -
Page 14: Installation Performance
Figure 2.3 UVWin interface 2.4 Installation performance Performance checks of the instrument should be carried out after installing the instrument. During the course of its life, periodical tests should also be made. The checks should only be carried out after preheating the instrument for at least 60 minutes after switching on. - Page 15 Table 2-1 656.1nm characteristic spectrum Scan parameter Comment Photometric Energy for sample light Scan speed Medium Sample interval Automatic Energy condition D2 lamp, gain 2 /3 Reference 660 650 nm Wavelength range Vertical coordinate 0 100 range Instrument parameter Comment Spectrum 2.0nm Fixed or variable...
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Page 16: Flatness Of The Baseline
2.4.2 Flatness of the baseline Testing method: Check that the sample beam is blank (empty with no solution). Then set the measuring parameters by using the spectrum scan function (refer to the UVWin software manual) as shown in table 2-3 Table 2-3 measure the flatness of baseline Scan parameter Comment... -
Page 17: Chapter 3 Instrument Operation
Chapter 3 Instrument operation All of the operation of T8DS is performed by the UVWin software after connection with the PC. Please read the UVWin software manual in detail before operating. This chapter will introduce the character of double beam measurement, and the process of operation of the T8DS using three examples. -
Page 18: How To Use A Double Beam Instrument
Double beam is read just once for reference and sample. For double beam instruments, put the black block into sample light beam cell holder to do the dark current correction. You can familiarize yourself with the operation of the T8DS by the following examples. -
Page 19: Application Examples
Power on the instrument Power up the PC which is connect with the T8DS, enter into the Windows operating system. Be sure there is nothing blocking the beam of the sample cell holder, and power on the instrument. Start UVWin software and allow the instrument to initialize. -
Page 20: Spectrum Measurement Example
Vitamin B12’s characteristic absorption peak is at 361nm, so the quality determination method of Vitamin B12 is to measure the absorbance at 361nm. The detailed operation steps of determining the absorption peak of Vitamin B12 by T8DS is as follows:... - Page 21 Power on the instrument Power up the PC which is connected with the T8DS and enter into the Windows operating system. Be sure there is nothing in the sample cell holder to block the beam and power on the instrument. Start UVWin software and allow to initialize.
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Page 22: Quantitative Measurement Example
[Cr(VI)] detection is an important item for the detection for electronic and electrical products. Take Diphenylcarbazide. The UV/Vis spectrophotometer method can detect the concentration of [Cr(VI)] in electronic and electrical products. The detailed operation of determining the concentration of [Cr(VI)] by T8DS is as follows: Prepare reagent First prepare the following solution. - Page 23 Power on the instrument Power up the PC which is connected with the T8DS and enter into the Windows operating system. Make sure there is nothing blocking the sample beam and power on the instrument. Start UVWin software and the instrument will carry out the initialization.
- Page 24 Setting parameters Select the “Quantitative” function and enter into the parameter setting interface. Set the parameters as follows: Table 3-2 Quantitative measurement parameter settings Measurement parameters Comments Measure method Single wavelength Main WL 540nm Calibration curve Abs=f(c) Equation Order Conc. Unit mg/L Calibration Concentration...
- Page 25 Table 3.3 Calibration Curve The parameters of calibration curve are: Abs = K1*(C) + K0 K0=0.0014 K1=0.68847 R2=0.9999 Measure the sample Input the ID of the sample in the “Sample” table using the same conditions and measure the Abs of sample solution and blank solution. The software will calculate the concentration of [Cr(VI)] by using the calibration curve, expressed as A and B.
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Page 26: Chapter 4 Optional Accessories
Chapter 4 Optional accessories 4.1 CH19-1 Thermostatic cell holder The thermostatic cell holder is used together with a constant-temperature water bath and water pump. It is designed to measure samples at a constant and accurate temperature. The picture and specification are shown below. Figure 4.1 Thermostatic cell holder Table 4.1 specifications Item... -
Page 27: Ds19-1 Angle-Changeable Solid Sample Holder
DS19-1 Angle-changeable solid sample holder Sliced solid samples can be installed onto the angle-changeable solid sample holder. The angles can be adjusted within ±45° and it is easy to measure the absorbency for solid samples.. The picture and specification are shown below. Figure 4.2 Angle-changeable solid sample holder Table 4.2 Specifications Item... -
Page 28: Is19-1 Integrating Sphere
IS19-1 Integrating sphere The integrating sphere is used to measure the diffuse reflectance of solid sample and powder, as well as measure the transmittance of glass and membrane. The integrating sphere can analyze the chrominance, color difference and whiteness. The picture and specification are shown below. Figure 4.3 Integrating sphere Table 4.3 Specifications Item... -
Page 29: Ls19-1 Long Pathlength Cell Holder
LS19-1 Long pathlength cell holder The long pathlength cell holder can be used with 5-50mm wide cells making it is easy for the measurement of samples. The picture and specification are shown below. Figure 4.4 Long pathlength cell holder Table 4.4 Specifications Item Specifications Remark... -
Page 30: Mh19-1 Micro Cell Holder
MH19-1 Micro cell holder Micro cuvettes can be used in the micro cell holder. The bases can be carefully adjusted to enable the light path to pass through the cell windows giving maximum light throughput and improve measurement precision for micro samples. The picture and specification are shown below. -
Page 31: Mh19-2 Ultra-Micro Cell Holder
MH19-2 Ultra-micro cell holder Ultra-micro cuvettes need to be used with the ultra-micro cell holder due to the clear aperture on the cuvettes being very small. Using a standard cell holder for the measurement would block some light causing the energy to be lost and the instrument precision reduced. -
Page 32: Mr19 1 Specular Reflection Accessory
MR19 1 Specula reflection accessory The specula reflection accessory can measure the reflectance spectrum of optical surfaces, crystals and coated samples. If the reflecting mirror on the reference beam is the “standard reflecting mirror”, the user can calculate the absolute reflectivity of the measured surface. The picture and specification are shown below. -
Page 33: Ms19-1 Manual 8-Cell Holder
MS19-1 Manual 8-cell holder Manual 8-cell holder is ideal for multi-sample measurements. Up to 8 samples can be put in the cell holder for separate measurement. The picture and specification are shown below. Figure 4.8 Manual 8-cell holder Table 4.8 Specifications Item Specifications Remark... -
Page 34: Ps19-2 Sipper Pump
PS19-2 Sipper pump The sipper pump accessory consists of a sipper pump, front baffle and connection tubes. The sipper pump is a soft-tube pump that can transmit liquid in dual directions. It is widely used in research and production for refine chemistry, biophysics, biochemistry, environmental protection, pharmaceuticals and other industries. - Page 35 Figure 4.9 Sipper Pump Table 4.9 Specifications Item Specifications Remark Product model PS19-2 Clockwise & 0.1-250rpm Rotation speed range counterclockwise 0.1rpm: lower than 30rpm Speed resolution 1 rpm: higher than30rpm 3 1/2 LCD display Display mode 1 mL Sampling accuracy AC85v-264v, 50-60Hz Power supply Temp: 0-400C,...
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Page 36: S19-1 Solid Sample Holder
S19-1 Solid sample holder Sliced solid samples can be put in the solid sample holder which makes it easy to measure the absorbency. The picture and specification are shown below. Figure 4.10 Solid sample holder Table 4.10 Specifications Item Specifications Remark Product model S19-1... -
Page 37: Ss19-1 Short Pathlength Cell Holder
SS19-1 Short pathlength cell holder The short pathlength cell holder is inserted into the standard cell holder making is easy to measure samples using short pathlength cells. The short pathlength cell holder can be used in the following holders: standard fixed cell holder, 8-cell holder, micro cell holder and ultra-micro cell holder. -
Page 38: Tr19-1 Test Tube Cell Holder
TR19-1 Test tube cell holder The test tube holder is used for the measurement of liquids contained in test tubes. The measurement results are dependent on the material and quality of the test tubes. Therefore if precise measurement is required, it is recommended that the related accessory for cuvettes be used. -
Page 39: Chapter 5 Maintenance
Chapter 5 Maintenance The T8DS UV-VIS Spectrophotometer is a precision instrument. Careful assembling and final test have been carried out before delivery. Appropriate routine attention and maintenance will not only ensure the reliability and stability of the instrument but also extend its operating lifetime. -
Page 40: Trouble Shooting And Maintenance
5.3 Trouble shooting and maintenance The instrument begins to initialize after turning on and connecting with the PC. The system gives status information after each operation has been completed. If no abnormality is detected, it displays “OK”. If an abnormality is detected, it displays “Err” and asks whether the initialization should continue or not. - Page 41 Table 5.1 Trouble shooting Phenomena Causes Diagnoses Remedy Power cable is not Check the power Connect it securely securely connected cable The instrument does not operate at all, after the Fuse is blown Check the fuse Replace the fuse (2A) power is turned on.
- Page 42 Note: If the initialization is abnormal, please confirm as the following steps first and then operate according to the upper table. Check the cable that connects the main unit with the PC. Power off (or quit the OS), initialize again, and check if the same error still appears.
- Page 43 Figure 5.2 Wavelength mechanism...
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Page 44: Appendix 1 Light Source Replacement
Appendix 1 Light source replacement Replace the tungsten lamp The tungsten lamp is plugged in to a socket and sleeved by a plastic holder. It is well adjusted before delivery. If you need to replace the tungsten lamp, please observe the following procedures to replace it. - Page 45 Figure 6.3 Loose screws Replace with a new tungsten lamp. Clean the surface of the tungsten lamp with a clean cloth soaked in alcohol. Insert the tungsten lamp holder into the tungsten lamp socket in the light source housing and tighten the two holding screws.
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Page 46: Replace The Deuterium Lamp
Replace the Deuterium lamp The D2 lamp is plugged in the D2 lamp socket. When replacement is required, insert a new one into the socket. The procedures is shown as follows: Open the light source cover. Remove the deuterium lamp by pulling it upwards. It may be installed tightly. Figure 6.5 Remove the D2 lamp Clean the surface of D2 lamp with a clean cloth soaked in alcohol. - Page 47 Note: 1 The light source and light source housing can become heated during operation, therefore, be sure to turn off the power switch and allow to cool down sufficiently touching. 2 The convergent lens should be covered to avoid being contaminated by fingers or objects while work is carried out..
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Page 48: Appendix 2 Fuse Replacement
Appendix 2 Fuse replacement This chapter introduces the user how to replace the fuse. If you suspect the fuse is blown, please replace it according to the following procedures. Switch off the instrument. Remove the fuse holder using a plain screwdriver, as shown in figure 6.1 Figure 6.1 Removing the fuse Remove the old fuse, and replace it with a new one. - Page 49 Persee Analytics Inc. Address:7270 Popham Place, Solon, OH44139, United States Contact:Sean Cao Title:Sales and Marketing Director E-mail:jia.cao@pgeneral.com Tel:(440) 591 9268 Web: www.perseena.com...