HEKA EPC 10 USB User Manual
  1. Manuals
  2. Brands
  3. HEKA Manuals
  4. Laboratory Equipment
  5. EPC 10 USB
  6. User manual

HEKA EPC 10 USB User Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
EPC 10 USB
USER MANUAL
EPC 10 USB Manual, Version 3.0

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the EPC 10 USB and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for HEKA EPC 10 USB

Summary of Contents for HEKA EPC 10 USB

  • Page 1 EPC 10 USB USER MANUAL EPC 10 USB Manual, Version 3.0...
  • Page 2 Germany Email eimeaorderscmt@harvardbioscience.com support@heka.com HEKA Electronic Phone +1 800 597 0580 84 October Hill Road +1 508 429 5732 01746 Holliston Website www.heka.com www.elproscan.com Massachusetts Email us-sales@smart-ephys.com United States support@heka.com EPC 10 USB • March 2022 • www.heka.com...

  • Page 4: Table Of Contents

    Rear-panel ..........12 EPC 10 USB DOUBLE, TRIPLE, and QUADRO ....... 12 Special Features of the EPC 10 USB .
  • Page 5 Testing the EPC 10 USB with the Model Circuit .......
  • Page 6 13.5 Connecting Cables ..........76 13.5.1 DG 4/5 / Lambda 10-2 / Lambda 10-3 to EPC 10 USB ..... . 76 13.5.2 ValveLink 8 to EPC 10 USB .
  • Page 7 16.2 SMA-Type ........... . 84 EPC 10 USB • March 2022 • www.heka.com...
  • Page 8 EPC 10 USB HDMI Red Star Headstage ........
  • Page 9 Bath electrode ..........63 13.1 Digital I/O connector (40-Pin, male) of the EPC 10 USB ......74 13.2 Digital In connector (DSub-25, female) of the EPC 10 USB .
  • Page 10 Number of free DA/AD channels in EPC 10 USB Multi amplifiers....13 Current Gain ranges of the EPC 10 USB amplifier......32 Filter 1 setting according to the defined Series Resistance Compensation speed .

  • Page 12: General Information

    Warning: The device and the software are not intended for medical uses and must not be used on hu- mans. HEKA Elektronik GmbH assumes no responsibility in any case of contravention. The product has been built to the state of the art and in accordance with recognized safety engineering rules. The device may only •...
  • Page 13 Do not try to insert anything sharp or metallic into the vents or the case. Liquids may cause short circuits or other damage. Always keep the device and the power cords dry. Do not handle it with wet hands. EPC 10 USB • March 2022 • www.heka.com...
  • Page 14 HEKA Elektronik GmbH makes no guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software. It is up to the user to use good laboratory practice to establish the validity of his / her findings.
  • Page 15 1.1. Important Safety Advice EPC 10 USB • March 2022 • www.heka.com...

  • Page 16: Introduction

    Figure 2.1: EPC 10 USB Patch Clamp amplifier The EPC 10 USB represents roughly the tenth in the series of patch clamp designs in use in the Göttingen lab- oratories. It is a logical successor to the classical EPC 7, retaining all of its features but adding a number of capabilities.

  • Page 17: References

    This manual is designed to provide a general guide for setting up and using the EPC 10 USB for experiments. It covers general information about the hardware, basic principles of the EPC 10 USB’s functions and patch clamp techniques.

  • Page 18: Naming Conventions

    MS Windows versions, whenever it is required. A free USB 2.0 port is required. 2.5 Macintosh The EPC 10 USB USB is supported by Macintosh computers running Mac OS X 10.6 or newer. A free USB 2.0 port is required.
  • Page 19 2.5. Macintosh EPC 10 USB • March 2022 • www.heka.com...

  • Page 20: Description Of The Hardware

    Figure 3.1: EPC 10 USB HDMI Red Star Headstage The probe of the EPC 10 USB is contained in a small enclosure, designed to be mounted on a micromanipulator and directly attached to the recording micropipette. It contains the sensitive amplifier that constitutes the current- to-voltage converter, as well as components for injecting signals into that amplifier.

  • Page 21: Main Unit

    3.3. Main Unit 3.3 Main Unit The main unit of the EPC 10 USB contains the power supply, the signal processing electronics, the A/D and D/A converters and the connectors for analog and digital input/output. Essentially all of the calibration adjustments are made by digital switches in the main unit, including those which depend on the properties of components in the probe.
  • Page 22 Digital Bus: This LED lights whenever digital information is sent from the computer to the EPC 10 USB amplifier. Note: Prior to the initialization of the amplifier the status of the Clipping and the Digital Bus LEDs is irrelevant.

  • Page 23: Rear-Panel

    (discontinued) to the EPC 10 USB amplifier. Trigger: This port does not exist any more. Use Trigger In on the front panel of the EPC 10 USB. USB: This port is the connection to the USB 2.0 port in the host computer, that allows the computer to communi- cate with the EPC 10 USB.

  • Page 24: Special Features Of The Epc 10 Usb

    Figure 3.4: Double Staggered Acquisition Mode of the DAQ board. Double Staggered Acquisition (EPC 10 USB using the LIH 8+8): The EPC 10 USB uses the double staggered acquisition mode. To support cophasic acquisition of the two most relevant signals, e.g. the current and voltage trace of the amplifier, two AD converters are used in parallel.
  • Page 25 3.5. Special Features of the EPC 10 USB EPC 10 USB • March 2022 • www.heka.com...

  • Page 26: Installation

    4.3 Conventions Software: All software packages of HEKA use a common installation procedure. Therefore, throughout the present manual, we will address the program to be installed as S...

  • Page 27: Connecting The Hardware

    ARDWARE another. 5. It is possible to “Join” two units (only for EPC 10 USB and LIH 8+8) to extend the number of analog channels controlled by one instance of the P software. For proper...

  • Page 28: Protection Key ('Dongle') Installation

    . Please pay attention to the supported OS and the according Re- OFTWARE lease Notes. If you have questions or doubts regarding the hardware/software compatibility please refer to the description of the product or contact the HEKA support hotline. Please download the latest software version from our website.

  • Page 29: Macos

    Calibration depends on temperature, we advice to let the H warm up for 30-60 minutes after power- ARDWARE ing it on. The Calibration procedure can be performed with any HEKA S . In the program, go to the OFTWARE Hardware menu (e.g. EPC 10 USB or PG 340 USB) Test and Calibrate Calibrate.

  • Page 30: Support Hotline

    Finally, the software re-initializes the amplifier. 4.8 Support Hotline If you have any question, suggestion, or improvement, please contact HEKA’s support team. The best way is to send us an e-mail specifying: • Your postal address and e-mail address •...
  • Page 31 4.8. Support Hotline • The series number and version of your H , if applicable: e.g. EPC 10 USB Single, serial number ARDWARE ’520552’, revision letter ’R’. • The questions, problems, or suggestions you have • Under which conditions and how often the problem occurs •...

  • Page 32: Amplifier Window

    The following screenshot provides an example how the Amplifier Window will look like when you start EPC 10 USB with the default settings (using an EPC 10 USB amplifier): Figure 5.1: Amplifier Window of EPC 10 USB with default settings while using an EPC 10 USB D patch OUBLE clamp amplifier.

  • Page 33: Global Section

    R-pip (pipette resistance) data parameter field. One can use this function to store the pipette resistance value in the data file before forming a seal. Reset: This function resets all hardware settings the E 10 USB to its default configuration. EPC 10 USB • March 2022 • www.heka.com...

  • Page 34: Tweak

    Figure 5.5: View tab of the Amplifier Window. Amplifer Boards: In the Amplifier Boards section one can deselect amplifier boards not needed for the experi- ment. E.g. if one is using an EPC 10 USB D amplifier and only one amplifier board is need one could OUBLE EPC 10 USB •...

  • Page 35: Hardware Tab

    (bath sense) and the ground (ground) electrode without having a current flow through the reference electrode (bath sense). The reference electrode (bath sense) stays in equilibrium. The potential diference is added to the command potential for the working electrode. EPC 10 USB • March 2022 • www.heka.com...
  • Page 36 Therefore, you need to connect the external stimulus source to either the “External Stim. Input CC” or “External Stim. Input VC” on the front panel of the EPC 10 USB amplifier. The signal is internally added to the holding current or holding potential of the EPC 10 USB. The entry field provided here, defines the scaling factor (range: 0.1 –...

  • Page 37: Tools Tab

    J Neurosci Meth 99, 129-135. V-mon: Copies the value of the V-mon parameter into the Holding field of LFVC. 5.1.1.3 Tools Tab In the Tools tab essential amplifier functions such as calibrating or testing the amplifier. EPC 10 USB • March 2022 • www.heka.com...

  • Page 38: Tools Tab Of The Amplifier Window

    MC 10. For an EPC 10 USB Single amplifier the whole procedure will take around 10 minutes. When the process has finished a new scale file and a new C-fast table is written into the EEPROM of the EPC 10 USB amplifier.

  • Page 39: Compensation Tab

    5.1. Global Section Join Units: Via this function you can join two hardware units together. E.g. you can join any two EPC 10 USB amplifiers (Single, Double, Triple, Quadro) or one EPC 10 USB amplifier (Single, Double, Triple, Quadro) and one stand-alone LIH 8+8 or two LIH 8+8 units.
  • Page 40 Search Mode Delay The Search Mode (see Operating Modes in the EPC 10 USB manual) is essentially a repetitive automated pipette offset procedure at a holding potential of “0” mV. The delay specifies at what rate the Auto VPip Offset compensation is performed.

  • Page 41: Amplifier Selection

    10 USB amplifier, you will either get one, two, three or four amplifier selection rows. If one has joined two EPC 10 USB amplifiers, the number can increase up to eight amplifier selections. Figure 5.10: Amplifier Selection of an EPC 10 USB Double amplifier.

  • Page 42: Amplifier Parameters

    Figure 5.11: Amplifier Parameters of an EPC 10 USB amplifier. Recording Mode EPC 10 USB tries to use the physiological convention whenever stimulating or acquiring data: therefore, a downward current always reflects a cationic influx into the cell. However, for this to work properly you always have to instruct the program about the actual recording configuration.

  • Page 43: Current Gain Ranges Of The Epc 10 Usb Amplifier

    (5 MΩ, 500 MΩ, and 5 GΩ), corresponding to low, medium and high Current Gain ranges. The table below summarizes the main features and limitations of the Current Gain ranges: Table 5.1: Current Gain ranges of the EPC 10 USB amplifier. Medium...
  • Page 44 1-1.5 pF due to the residual input capacitance of the current measuring amplifier. The compensation can be performed manually by dragging the mouse or typing. Note: The range of the fast capacitance can be extended to 0-80 pF if an EPC 10 USB amplifier with revision letter ’N’ is used.
  • Page 45 The two parameters for the automatic compensation routine can be set in the Compensation tab of the Amplifier Window (see chapter 5.1.1.4 on page 28). EPC 10 USB • March 2022 • www.heka.com...

  • Page 46: Bridge Compensation Options In Current Clamp Mode

    Current Gain range. They are 2 nS, 200 nS and 20 µS for high, medium, and low ranges, respectively. The injection time constant is 100 µs. Note: Data acquisition in EPC 10 USB does not consider a possible hardware "Leak" cor- rection of the E 10 USB.

  • Page 47: Filter 1 Setting According To The Defined Series Resistance Compensation Speed

    If you want to use an external filter rather than Filter 2, you can proceed as follows (example given for an EPC 10 USB Single amplifier): Connect current monitor 1 (Imon1 ) to the external filter input, feed the filter output back into one of the AD channels, and select this channel as input channel.

  • Page 48: Verifying And Testing The Epc 10 Usb

    The following tutorial will guide you through most of the basic and some of the unique and more sophisticated features of the EPC 10 USB amplifier. At the same time it allows you to check, whether the amplifier is function- ing properly.

  • Page 49: Setup" - Applying The Test Pulse

    ). Have a look for the ATCHMASTER Amplifier Window which provides a graphical representation of the EPC 10 USB amplifier. The panel lets you control all hardware settings of the amplifier(s) such as Gain or Filters. Signal display of an applied "Test Pulse"...
  • Page 50 6.1. Testing the EPC 10 USB with the Model Circuit segment in the Oscilloscope and the I-mon display (10) match zero. In P similar steps as listed above can be automatically executed by clicking on the SETUP ATCHMASTER button in the Test Pulse window or pressing the 1 key on the numerical keypad. This will execute the following...

  • Page 51: Seal" - Voltage Clamp Recording

    6.1. Testing the EPC 10 USB with the Model Circuit 6.1.3 "SEAL" - Voltage Clamp Recording Now, move the switch of the model circuit to the center position which leaves only a capacitance of about 6 pF connected. This simulates a GΩ seal and the C-Fast controls can be used to cancel the capacitive spikes resulting from the stimulus test pulse.

  • Page 52: After C-Fast Compensation

    6.1. Testing the EPC 10 USB with the Model Circuit Figure 6.4: After C-Fast compensation The steps listed above can be automatically executed by clicking on the SEAL button or pressing the key "2" on the numerical keypad. This will execute the following predefined protocol lines that increases the gain and then performs twice an auto-compensation - considering a possible failure in the first attempt.

  • Page 53: Whole-Cell" - Voltage Clamp Recording

    6.1. Testing the EPC 10 USB with the Model Circuit 6.1.4 "Whole-Cell" - Voltage Clamp Recording After compensating C-fast well, you can now switch into the "0.5 GΩ" position of the model circuit. This will simulate a "model cell" with 22 pF "membrane capacitance", 500 M Ω "membrane resistance" and 5.1 M Ω "input resistance"...

  • Page 54: After C-Slow Compensation

    6.1. Testing the EPC 10 USB with the Model Circuit Activate the C-Slow compensation by selecting the 100 pF range from the Range field (15). Now, start the com- pensation by increasing the C-Slow (16) and the Series Resistance (RS) (17) values for both parameters. With some exercise you will get a better feeling for these parameters and how they effect the recording.
  • Page 55 6.1. Testing the EPC 10 USB with the Model Circuit The steps listed above can be automatically executed by clicking the WHOLE-CELL button or pressing the key "3" on the numerical keypad. This will execute the following protocol (see Protocol Editor) that sets the right Current...

  • Page 56: Whole-Cell" - Current Clamp Recording

    6.1. Testing the EPC 10 USB with the Model Circuit 6.1.5 "Whole-Cell" - Current Clamp Recording If C-Slow has been compensated so far, switch into the current clamp mode by selecting C-Clamp from the Recording Mode pop up. This automatically selects the voltage monitor (V-mon) as the active channel displayed in the Test Pulse window.
  • Page 57 Note: The 500 M Ω setting of the model circuit is not a good method for testing the fast clamp speed of the EPC 10 USB due to the long time constant of 11 ms which the amplifier can easily follow.

  • Page 58: Measuring The Noise Of The Amplifier

    It is recommended to click the Reset button in the Amplifier window in order to reset the EPC 10 USB to its initial default configuration. Now, go to the Tweak menu (21) of the Test Pulse window and select in the View pane the option Noise (22) to show the noise parameters on the bottom of the Test Pulse window.

  • Page 59: Making A Full Test

    BNC connector at the probe to shield it), a 10 M Ω resistor (e.g., the model circuit) and 5 short BNC cables. In case you have an EPC 10 USB Double, Triple or Quadro and you want to test only one amplifier board instead of all of them, you have to select the amplifier you want to test first in the Amplifier Window (active amplifier), before you start.
  • Page 60 Note: Please, remind: if you get a message like this, please, contact HEKA first and supply us the error protocol before sending the amplifier in. You might save yourself valuable time, money and effort! EPC 10 USB •...
  • Page 61 6.2. Making a Full Test EPC 10 USB • March 2022 • www.heka.com...

  • Page 62: Operating Modes

    The measured membrane potential will be shown on the V-mon display in the acquisition soft- ware. The same signal is available at the Voltage Monitor output at the front panel of the EPC 10 USB. For stimulation, a commanded current can be injected while the pipette potential is measured. The commanded cur- rent is determined by the sum of the voltages from the "External Stim.Input CC"...

  • Page 63: Current Clamp Mode

    19: 530-534). The current clamp circuit of the EPC 10 USB acts as a voltage-follower, thereby increasing not only speed but also stability of the circuit. This circuit causes the effective input resistance of the EPC 10 USB probe to be greater than 10 Ω.

  • Page 64: Low Frequency Voltage Clamp Mode

    Gain Range it is a hundred times faster. Note that the LFVC value of 100 means slow compensation, whereas the LFVC value of 1 means fast compensation! EPC 10 USB • March 2022 • www.heka.com...
  • Page 65 7.3. Low Frequency Voltage Clamp Mode EPC 10 USB • March 2022 • www.heka.com...

  • Page 66: Compensation Procedures

    This arrangement constitutes positive feedback, and can become unstable when overcompensation occurs. The EPC 10 USB incorporates additional circuitry to allow capacitance transient cancellation to occur while RS- compensation is in use (see Sigworth, Chapter 4 in Single-Channel Recording). This is shown as the prediction pathway in the figure below, and it accelerates the charging of the membrane capacitance by imposing large, transient voltages on the pipette when step changes are commanded (this is sometimes called "supercharging").

  • Page 67: Series Resistance Compensation Circuit Of The Epc 10 Usb

    Figure 8.1: Series Resistance Compensation Circuit of the EPC 10 USB. Together, the two parts of the EPC 10 USB RS-compensation circuitry cancel the effects of a fraction of the series resistance. This means that the charging of the membrane capacitance is accelerated, with a time constant under compensation of: τ...

  • Page 68: Capacitance Compensation

    Any maladjustment of the transient cancellation will be apparent and can be compensated. The EPC 10 USB makes the procedure very easy: C-slow and R-series values can be obtained automatically by clicking on Auto C-slow. Beyond that, the Pulse Generator of P...

  • Page 69: Offset Compensation

    LJ: A software variable which can be set by the user. It represents the sum of all "variable offsets" applicable at a given time (Offset Sum). In order for the variable LJ to actually represent the various offsets, and to correct for these, three conditions have to be met: EPC 10 USB • March 2022 • www.heka.com...

  • Page 70: Exemplary Solutions And Their Liquid Junction Potentials

    Example 2: An outside out or whole cell measurement with KCl-based internal solution in the pipette. LJ should be set to 3 mV (see table) in order to correct for the disappearance of a liquid junction potential between the KCl containing pipette and the NaCl-based bath solution. EPC 10 USB • March 2022 • www.heka.com...
  • Page 71 2. the resting potential of the cell. We assume the latter to be -60 mV and therefore set LJ to -54 mV In the cell-attached mode polarities of the amplifier readout are inverted, thus the amplifier will display the "physiological" patch potential. EPC 10 USB • March 2022 • www.heka.com...

  • Page 72: Patch Clamp Setup

    Because of the extreme sensitivity of the EPC 10 USB, special care must be taken in grounding all surfaces that will be near the probe input in order to minimize line-frequency interference.

  • Page 73: External Shielding

    (e.g., variable filter, Oscilloscope, Pulse Generator, data storage). However, for test purposes, it is still a good idea to have an oscilloscope connected to the EPC 10 USB. You may connect e.g. additional filter units or amplification units to Filter 1 of the current monitor. Also, it might be convenient to observe the voltage and current monitor signals at the recorder’s outputs, rather than directly from the patch clamp.

  • Page 74: Bath Electrode

    The filling solution can be a typical bath solution or something similar, such as 150 mM NaCl. More concentrated salt solutions are not necessary, and they can leak out, changing the composition of the bath solution. EPC 10 USB • March 2022 • www.heka.com...
  • Page 75 9.7. Bath Electrode EPC 10 USB • March 2022 • www.heka.com...

  • Page 76: Patch Pipettes

    (or in an oven at 50 C for 20 min) until it begins to thicken. It can then be stored at -18 C for many weeks until use. The Sylgard™ is applied around the lower few mm of the electrode to within 10 20 µm of the EPC 10 USB • March 2022 • www.heka.com...

  • Page 77: Heat Polishing

    Noise test function. If it increases, solution has probably spilled inside the holder; in this case the holder should be cleaned again and dried thoroughly. EPC 10 USB • March 2022 • www.heka.com...

  • Page 78: Using The Patch Clamp

    Application of gentle suction should increase the resistance further, and result (sometimes gradually, over maybe 30 s; sometimes suddenly) in the formation of a gigaseal, which is characterized EPC 10 USB • March 2022 • www.heka.com...

  • Page 79: Cell-Attached Recording

    10 kHz filter. Then, during the recording, watch to see if the clipping light blinks. When it does, it means that internal amplifiers in the EPC 10 USB are about to saturate, and/or that the current monitor output voltage is going above 10 V peak, on the peaks of the transients, and you should readjust the transient cancellation controls.

  • Page 80: Capacitive Transient Cancellation

    It should be adjusted with some care, since too high a setting causes overcompensation (the EPC 10 USB will think that RS is larger than it is); this can cause oscillation and possible damage to the cell under observation.

  • Page 81: Current Clamp Recording

    EPC 10 USB. When you switch to the current clamp mode, the following things happen inside the EPC 10 USB main unit: I-membrane is set to a value that will keep the membrane voltage constant, internal stimulation is stopped, the 10 kHz Bessel characteristic for Filter 1 is selected, C-Slow Range is set to "Off", and the value of C-fast is reduced...

  • Page 82: Low Noise Recording

    9 on page 61. The noise feature of the EPC 10 USB makes it easy for the user to identify important noise sources. When the Noise radio button is selected in the Test Pulse window of P...
  • Page 83 See the chapter by Marty and Neher (1983) for a more complete description of this and other fine points of whole cell recording. EPC 10 USB • March 2022 • www.heka.com...

  • Page 84: Appendix I: Digital Inputs/Outputs

    The digital In and Out lines of this connector carry TTL-compatible signals. This 40-pin connector is intended to connect other HEKA devices, such as EPC 8 or TIB 14 to the EPC 10 USB amplifier. For other purposes the Digital In connector and the Digital Out connector should be used.

  • Page 85: Digital In Connector

    The Digital In connector at the rear panel can be used to read TTL trigger signals from external devices. Table 13.2: Pin assignment of the Digital In Connector (EPC 10 USB), upper row. IN = Digital Input Line; GND =...

  • Page 86: Breakout Cables

    13.4. Breakout Cables Figure 13.3: Digital Out connector (DSub-25, female) of the EPC 10 USB. Table 13.5: Pin assignment of the Digital Out Connector (EPC 10 USB), lower row. OUT = Digital Output Line; GND = Ground Line PIN # Signal OUT-4 OUT-5 OUT-6 OUT-7 OUT-8 OUT-9 OUT-10 OUT-11 OUT-12 OUT-13 OUT-14 OUT-15 13.4 Breakout Cables...

  • Page 87: Connecting Cables

    Figure 13.5: DSub-25 (male) to DSub-25 (female) connecting cable Table 13.6: Pin assignments of Digital Out Connector of EPC 10 USB and Parallel Port connector of DG 4/5. OUT = Digital Output Line; GND = Ground Line; NC = Not Connected...

  • Page 88: Pin Assignments Of Digital Out Connector Of Epc 10 Usb And Parallel Port Connector Of Lambda

    13.5. Connecting Cables Table 13.7: Pin assignments of Digital Out Connector of EPC 10 USB and Parallel Port connector of Lambda 10-2. OUT = Digital Output Line; GND = Ground Line; NC = Not Connected EPC 10 USB Lambda 10-2...

  • Page 89: Valvelink 8 To Epc 10 Usb

    On request there is a connecting cable available to connect the ValveLink 8 controller (order number: 895113). Figure 13.6: DSub-25 (male) to DSub-9 (male) connecting cable Table 13.9: Pin assignments of Digital Out Connector of EPC 10 USB and connector of ValveLink 8. OUT = Digital Output Line; GND = Ground Line; NC = Not Connected...

  • Page 90: Appendix Ii: Probe Adapters

    Figure 14.1: Standard Mounting Plate for EPC 10 USB pre-amplifiers. 14.2 Dovetail Mounting Plate The dovetail adapter (order number: 895020) can be used to connect e.g. to Sutter MP 285 or HEKA MIM 4 micromanipulator. The dimensions of the dovetail match the probe housing.
  • Page 91 14.2. Dovetail Mounting Plate EPC 10 USB • March 2022 • www.heka.com...
  • Page 92 The pipette holder may be tied with fingers only. Do not use tools to tighten it! The headstage can be used either with an EPC 10 (revision ’O’ or higher) or an EPC 10 USB (revision ’N’ or higher) or the EPC 800 USB amplifier missing with 3 Electrode mode though.
  • Page 93 EPC 10 USB • March 2022 • www.heka.com...
  • Page 94 16 Appendix IV: Pipette Holder Due to the fact that the EPC 10 USB can be used either with the Red Star Headstage or the S-Probe different types and sizes of pipette holders are available. More information about the different pipette holders and their accessories can be found on the HEKA website.

  • Page 95: Pipette Holder Sma-Type For The S-Probe

    • Pipette Holder SMA-Type 1.5 mm (Order number: 895150) • Pipette Holder SMA-Type 1.7 mm (Order number: 895151) • Pipette Holder SMA-Type 2.0 mm (Order number: 895152) Please contact eimeaorderscmt@harvardbioscience.com to get further information on this product. EPC 10 USB • March 2022 • www.heka.com...
  • Page 96 Hardware Options, 24 EPC 10 Amplifier Hardware Tab, 24 C-Clamp, 31 Low Frequency Voltage-Clamp, 26 In Out, 31 Tools Tab, 26 On Cell, 31 View Tab, 23 Out Out, 31 Appendix III EPC 10 USB • March 2022 • www.heka.com...
  • Page 97 Low Frequency Voltage Clamp, 53 Description of the HardwareRear-panel Voltage Clamp, 51 Power, 12 Digital Inputs/Outputs, 73 Patch Clamp Setup, 61 Breakout Cables, 75 Bath Electrode, 63 Connecting Cables, 76 Electrode, 62 Digital I/O Connector, 73 EPC 10 USB • March 2022 • www.heka.com...
  • Page 98 Current Clamp Recording, 70 Forming a Seal, 67 Entering the Bath, 67 Forming a Gigaseal, 67 Initial Setup, 67 Whole-Cell Recording, 68 Breaking the Patch, 68 Capacitive Transient Cancellation, 69 Series Resistance Compensation, 69 EPC 10 USB • March 2022 • www.heka.com...

Table of Contents