HEKA PG 310 Manual
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HEKA PG 310 Manual

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Manual 1.0
PG 310/PG 390
PG 340
Potentiostat / Galvanostat

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Summary of Contents for HEKA PG 310

  • Page 1 Manual 1.0 PG 310/PG 390 PG 340 Potentiostat / Galvanostat...
  • Page 2 Canada support@heka.com HEKA Instruments Inc. Phone +1 516 882-1155 2128 Bellmore Avenue +1 516 467-3125 Bellmore, New York Web Site http://www.heka.com 11710-5606 U.S.A. Email ussales@heka.com support@heka.com Title Page: Potentiostat/Galvanostat PG 310 © 2004-2008 HEKA Elektronik Dr. Schulze GmbH COMDP9/1...

  • Page 3: Table Of Contents

    1 Introduction 1.1 Introducing the PG 310, PG 390, and PG 340 ..1.2 Naming Conventions ..... .
  • Page 4 3.1 Connecting the PG 300 ....4 The Control Software 4.1 Controlling the PG 310/390 - Main Functions ..4.1.1 Cell Connection .
  • Page 5 Digital In Connector ....6.2.3 Digital Out Connector ....http://www.heka.com...

  • Page 7: Introduction

    1. Introduction Introducing the PG 310, PG 390, and PG 340 The PG 300 series potentiostat/galvanostat feature state-of-the-art ampli- fier technology. They include amplification ranges for large currents, new hardware filters and an external preamplifier design (optional) to reduce noise levels for small currents. The most significant advance is the complete software controlled adjustment of the amplifier.

  • Page 8: Naming Conventions

    Naming Conventions PG 310 USB, PG 390 USB, and PG 340 USB Throughout the present manual we will address all three amplifier types as “PG 300”. We will explicitly mention the particular amplifiers, where it is required.

  • Page 9: Product Intended Usage

    Users are expected to be able to properly operate the PG 300 and associated instruments. The HEKA PG 300 is not intended for medical use. The HEKA PG 300 is not intended for use in life support situations, or in situations were improper operation or failure of the PG 300 can result in personal injury.

  • Page 10: Unpacking

    After unpacking the PG 300 and accessories from the shipping carton, please inspect each piece for any signs of shipping damage. Please con- tact the delivering carrier and HEKA immediately if there is any damage. Do not dispose of the shipping carton. The carrier will want to examine the shipping carton to process a damage claim.

  • Page 11: Support Hotline

    (PCI, PC Slot, etc). Support Hotline If you have any question, suggestion, or improvement, please contact HEKA’s support team. The best way is to send us an e-mail or fax speci- fying: ˆ Your postal and E-mail address (or fax number) ˆ...
  • Page 12 Introduction http://www.heka.com...

  • Page 13: Description Of The Hardware

    2. Description of the Hardware Characteristics 2.1.1 Power Requirements AC-power 110 V or 220 V, 50 to 60 Hz, 170 W maximum (PG 310) 260 W maximum (PG 390) 2.1.2 Dimensions Housing 43 cm W x 18 cm H x 35 cm D (16.9”...

  • Page 14: Hot-Swappable

    The number of units that can be installed is only limited by the number of available Hi-Speed USB 2.0 ports. 2.1.6 DC Characteristics PG 310 PG 390 Counter Electrode: Compliance Voltage 20 V...

  • Page 15: Potentiostatic Mode

    Noise measured with open input: 8-pole Bessel filter; 1 nA - 100 pA, 500 MΩ? feedback resistor: DC to 10 kHz: ¡ 400 fA (RMS) Maximum bandwidth: 100 kHz 2.1.9.2 Filters The PG 310/PG 390 contains three built-in filters. http://www.heka.com...

  • Page 16: Initial Potential

    This sensing applies for any current change regardless of its source. This includes the PG 300 EXTERNAL INPUT. Note: The EXTERNAL INPUT has 5 KΩ input impedance in both the potentiostatic and galvanostatic modes. http://www.heka.com...

  • Page 17: Main Unit

    POTMASTER program. However, this program allows you to re-initialize the amplifier in case you forgot to turn it on first. Note: Since the calibration settings of the potentiostat have been determined for a warmed-up potentiostat, switch on the http://www.heka.com...
  • Page 18 DA template for the first trigger is loaded first. The non-zero values for the following triggers are then added, i.e. in case of overlapping the DA values will become additive. DA-2 is also http://www.heka.com...
  • Page 19 In the four-electrode mode, connect your first reference electrode here. Reference II: In the four-electrode mode, connect your second reference electrode here. In the three-electrode mode, Reference II is not used. Working Electrode: Connect your working electrode here. http://www.heka.com...
  • Page 20 It is particularly useful as it will indicate clipping by internal amplifiers even if the output voltage is not saturated, because of filtering. Moreover, this LED lights whenever digital information is sent from the computer to the PG 300potentiostat. http://www.heka.com...

  • Page 21: Simplified Block Diagram

    Although the PG 300potentiostat/galvanostat is a complex instrument, the organization of its analog part is straightforward and depicted in the following figure. The succeeding paragraphs discuss the PG 300potentio- stat/galvanostat, in combination with the different operating modes, in some detail. http://www.heka.com...
  • Page 22 Description of the Hardware http://www.heka.com...

  • Page 23: Installation

    3. Installation Connecting the PG 300 1. The PG 300 can be installed into a standard nineteen-inch instru- ment rack or used as a desktop unit. If installing on a rack, please do not use the PG 300 as a shelf to support any other instrument. The PG 300 case was not designed to do this and damage to the front panel will result.
  • Page 24 LED will illuminate once the PG 300 is powered ON. Important note: Please note that the Status LED will not be illuminated until the acquisition software has initialized the interface. The PG 300 is now connected and ready to go. http://www.heka.com...

  • Page 25: The Control Software

    4. The Control Software The Potentiostat window is used for controlling, adjusting and displaying the amplifier settings. The window might differ depending on the type...

  • Page 26: Controlling The Pg 310/390 - Main Functions

    Potmaster. We will describe the window of the PG 300 in most detail and focus to special details only when describing the windows of other amplifiers. Controlling the PG 310/390 - Main Func- tions The PG 300Potentiostat window is used for controlling, adjusting and displaying the Pg 310/390 Potentiostat/Galvanostat operating modes.

  • Page 27: Potential / Current Settings

    Pg 310/390 Potentiostat, and the current flowing through the cell is displayed as I-Cell. In the galvanostatic mode the current flow through the cell is controlled by the Pg 310/390 Galvanostat, and the respective cell potential is shown in the E-Cell display.
  • Page 28 To set the current range, click with the mouse on the Current Range button and selecting the desired range in the pop-up window by tagging. If the high gain pre-amplifier is connected to the Pg 310 Potentio- stat/Galvanostat, the current ranges from 100pA to 100nA are se- lectable.

  • Page 29: Filter Settings

    4.1 Controlling the PG 310/390 - Main Functions Charge Reset: Resets both the anodic and the cathodic charge. Amplifier Mode: Allows a selection between potentiostatic and galvanostatic mode. 4.1.3 Filter Settings Notch Filter: 310/390 Potentio- stat/Galvanostat provides a notch filter for both the voltage and the current pathway.
  • Page 30 10 kHz 30 kHz 100 kHz The possible settings depend on the Pg 310/390 Potentiostat version: ˆ If you are using a Pg 310/390 Potentiostat/Galvanostat with a ”A” or ”B” labeled serial number, the low frequency bandwidth of 10 Hz will not be available.
  • Page 31 4.1 Controlling the PG 310/390 - Main Functions Note: In a ramp segment the stimulus change is step-like rather than line-like. The number of steps needed for represent- ing the ramp is defined by the quotient of the segment length and the ”sample interval”...

  • Page 32: Additional Settings

    Initial Potential externally (e.g., with a stimulator or another computer). Reset: Selecting this button will reset the Pg 310/390 to its initial de- fault configuration. Reset is very useful for defining the initial state of the Pg 310/390, when recording a macro.

  • Page 33: Cell Mode

    The 3 Electrode Mode consists of a working, a counter and a reference electrode. In this mode the second reference electrode input (Reference II) on the front panel of the Pg 310/390 is short-circuited to the working electrode, so that the potentiostat controls the voltage between the working and reference electrodes.

  • Page 34: Macro Recording

    You have the option of actually executing all actions as they are entered, or of disabling execution and only logging the actions to the macro. This option you have to set in the PGF-Editor menu Macro options before you record. http://www.heka.com...
  • Page 35 4.1 Controlling the PG 310/390 - Main Functions Record: To start macro recording, click on the Record button. Then, perform all desired actions. (The Notebook window will print a protocol of the macro actions.) You may record up to 50 actions in the PG 300, Oscilloscope and Online Analysis windows.

  • Page 36: Hidden Controls

    This is the default setting. If the function Ampl. Mode switch: keep E/I-init is chosen, current or voltage leaps may occur and affect the electrochemical processes at the working electrode. http://www.heka.com...

  • Page 37: Features For Macros

    The time in seconds for the Wait function is set in the field above. Delay: This determines how many seconds the program has to wait before the next action is started by the Pg 310/390 Potentio- http://www.heka.com...

  • Page 38: Controlling The Pg 340 - Special Functions

    Using two current chan- nels strongly different currents can be simultaneously recorded. The bipo- tentiostat Pg 340 is therefore the heart of the HEKA ElProScan, the new developed Electrochemical Probe Scanner which is much more then a SECM.

  • Page 39: Electrode Conditions

    DISK or RING - is exhibited in the E-Cell display. The small Set insert button is used to set the Initial Potential value to the actual Open Cell Potential. Note: It is also possible to switch to the Standby mode by pressing the ‘0’ key of the numeric keypad. http://www.heka.com...

  • Page 40: Potential/Current Settings

    The Control Software 4.3.3 Potential/Current Settings Almost all functions are the same as for the standard Pg 310/390 Postentiostat/Galvanostat, see Chapter 4.1 PG 310/390 Potentio- stat/Galvanostat. However, there are some small differences to be taken into account. All settings apply to the selected electrode, e.g., DISK or RING.
  • Page 41 (DISK and RING) and reference electrodes. This commonly used mode is the default setting. The 4 Electrode Mode provides two distinct reference electrodes (Refer- ence I and Reference II on the front panel of Pg 340), between which the potentiostat controls the voltage. http://www.heka.com...
  • Page 42 The Control Software http://www.heka.com...

  • Page 43: Operating Modes

    5. Operating Modes The PG 500 is fundamentally an instrument for controlling cell potential or current and measuring electric currents and potentials. In low current ranges it uses a current-to-voltage (I-U) converter circuit to convert the currents to an analog voltage, which is then made available at the current monitor output for display or recording.

  • Page 44: Potentiostatic Mode

    fixed at the potential of the signal lead guards the reference electrode connector. This is realized by an electrometer amplifier that feeds the signal potential to the lead shield, that is, the outer part of the BNC http://www.heka.com...

  • Page 45: Current Amplifier

    The bandwidth of the control amplifier is selected within the limits of 30 Hz to 100 kHz by changing a capacitor in the feedback loop. The maximal bandwidth (MAX) with non-capacitive feedback is defined by the internal circuit of the operational amplifiers and http://www.heka.com...
  • Page 46 In this model the desired potential difference between the working and ref- erence electrodes is maintained at 0.100 V. As a result of IR compensation, however, this potential difference no longer includes a voltage drop error due http://www.heka.com...
  • Page 47 IR compensation procedure must be performed for each cur- rent range used. The highest compensation resistance (Rcomp max.) and the resolution of the compensation resistance are a function of the current range. http://www.heka.com...

  • Page 48: Galvanostatic Mode

    I-U converter provided for high and low current ranges, the bandwidth control of the control amplifier and the summing of the various input signals, also applies to galvanostatic operation. The input signals, consisting of an Initial Current, a generated Scan and an http://www.heka.com...

  • Page 49: Ir Compensation

    IR compensation will act as an additional voltage to the U-Cell Monitor ; it is determined by the setting and the output signal from the current amplifier. compensation http://www.heka.com...
  • Page 50 IR compensation procedure has to be performed for each current range used. The highest compensation resistance (R max.) comp and the resolution of the compensation resistance are a function of the current range, as follows. http://www.heka.com...

  • Page 51: Appendix I: Technical Data

    Digital I/O Connector The digital IN and OUT lines of this connector carry TTL-compatible signals. The connector is intended to connect other HEKA devices, such as the TIB 14 to the PG 300. For other purposes the Digital In connector and the Digital Out connector should be used.

  • Page 52: Digital In Connector

    Figure 6.2: Digital I/O connector of the PG 300 6.1.2 Digital In Connector The Digital In connector at the rear panel can be used to read TTL trigger signals from external devices. Figure 6.3: Digital In connector of the PG 300 http://www.heka.com...

  • Page 53: Digital Out Connector

    TTL inputs. The signals of pin 21 (OUT-0) to 23 (OUT-2) can also be accessed from the BNC trigger outputs at the front panel of the amplifier. Figure 6.4: Digital Out connector of the PG 300 http://www.heka.com...

  • Page 54: Usb Series

    Digital I/O Connector The digital IN and OUT lines of this connector carry TTL-compatible signals. The connector is intended to connect other HEKA devices, such as the TIB 14 to the PG 300amplifier. For other purposes the Digital In connector and the Digital Out connector should be used.

  • Page 55: Digital In Connector

    The Digital Out connector at the rear panel of the PG 300 amplifiers can be used to trigger external devices, which require TTL inputs. The signals of pin 21 (OUT-0) to 23 (OUT-2) can also be accessed from the BNC trigger outputs at the front panel of the amplifier. http://www.heka.com...
  • Page 56 Appendix I: Technical Data Figure 6.8: Digital Out connector of the PG 300USB http://www.heka.com...
  • Page 57 Index Ampl.Mode Switch, 30 Galvanostatic Mode, 9 Amplifier Initial Potential, 10 Potentiostatic Mode, 9 Bandwidth, 24 Power Requirements, 7 Amplifier Mode, 23 Weight, 7 Auto Filter, 23 Differential Amplifier, 38 Auto Range, 27 digital I/O, 11 Bandwidth, 24 E-initial, 21 Beep External Input, 26 Macro Execution, 31...
  • Page 58 Simplified Block Diagram Operating Modes, 37 Operating Modes, 15 overload, 14 Stimulus Filter, 24 Support Hotline, 5 PG 310/390 Potentiostat, 20 PG 340, 32 Three Electrode Mode, 27, 34 Additional Settings, 34 Cell Connection, 33 Voltage Filter, 25 Cell Mode, 34...

This manual is also suitable for:

Pg 390Pg 340

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