General Use; Using The Microscope - Levenhuk Discovery Atto User Manual

DISCOVERY ATTO MICROSCOPES

General use

The Discovery Atto microscopes are safe for health, life and property of the consumer and the environment when
properly used, and meet the requirements of international standards. The microscopes are designed for observing
transparent and opaque objects in the transmitted and reflected light using the bright field method.
They perform well in clinical research and tests; teaching demonstrations; bacterioscopy and cytology in medical
and health establishments, laboratories, universities; and may be used for scientific research in agriculture and
microbiology.
Caution! Children should use the microscope under an adult's supervision only.
Microscope assembly
• Unpack the microscope and make sure all parts are available.
• Move the stage to the lowermost position using the focusing knob.
• Make sure the batteries are correctly installed in the battery compartment; insert new batteries if required.
You can also plug the power cord into the microscope and then plug it in to the power source.
• Insert the eyepiece into the eyepiece tube.
• For the digital camera model: you can attach the digital camera to the eyepiece tube instead of the eyepiece.

Using the microscope

• Turn on the power and the illumination will turn on. Set the brightness at approximately 70%.
Place the specimen on the stage. Make sure the clips hold the specimen firmly in place.
• Iris diaphragm should be adjusted in accordance with numerical aperture of a lens. The best practice is
to make the iris diaphragm slightly smaller than the aperture of the selected lens. Open or close the iris
diaphragm using the adjustment screws. If the adjustment screw is turned to the right, the iris diaphragm
is fully open. Note: the diaphragm is not intended for adjusting the brightness of the illumination. To adjust
the brightness, use the brightness adjustment knob.
• Slide the filter holder, place the filter in it and then return the holder to its initial position.
• To change the objective magnification, turn the revolving nosepiece until it clicks.
• Make sure that the lens does not touch the sample when adjusting the focus: rotate the coarse focus knob
until the specimen is about 3.175mm (1/8") away from the lens.
• Looking into the eyepiece, slowly turn the coarse focusing knob until you can see the image of the observed
specimen. Turn the fine focusing knob to make the image clear. The fine focusing mechanism allows you to
focus on the observed specimen while using high magnifications.
• When you use an oil immersion objective, the space between the specimen and the lens should be filled with
immersion oil. Raise the condenser to the upper position and put a drop of oil on the lens and on the cover
glass of the specimen. Move the revolving nosepiece back and forth to get rid of air bubbles in the oil. Then,
fix the objective lens in its working position. Make sure that oil fills the entire space between the objective lens
and the specimen. After use, wipe the lens dry.
• To place the specimen in the field of view, move the stage horizontally back and forth or left and right using
the stage moving knob.
Selecting the objective
Start your observations with the lowest magnification objective and select a specimen segment for detailed
research. Then move the specimen to center the selected segment in the field of view, to make sure it keeps
centered when the objective is changed to a more powerful one. Once the segment is selected, you should center its
image in the microscope's field of view as precisely as possible. Otherwise, the desired segment might fail to center
in the field of view of the higher power objective. Now, you can switch to a more powerful objective by rotating the
revolving nosepiece. Adjust the image focus if required.
Digital camera (for the digital model only)
The digital camera is installed in the eyepiece tube in place of the eyepiece. It allows to observe specimens in fine
detail and true colors on your PC monitor and save images on the hard drive. The software allows viewing and
editing the object images.
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