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USER MANUAL
Flexcell
Flex Jr.
®
™
Tension System
11-08-2018
Revision 13.1
™
Culturing Cells in a Mechanically Active Environment
Flexcell International Corporation 2730 Tucker Street, Suite 200 Burlington, NC 27215
800-728-3714 (919) 732-1591 FAX: (919) 732-5196 www.flexcellint.com
COPYRIGHT © 2009 FLEXCELL
INTERNATIONAL CORPORATION
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Summary of Contents for flexcell Flex Jr.
- Page 1 11-08-2018 Revision 13.1 ™ Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street, Suite 200 Burlington, NC 27215 800-728-3714 (919) 732-1591 FAX: (919) 732-5196 www.flexcellint.com COPYRIGHT © 2009 FLEXCELL INTERNATIONAL CORPORATION...
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Page 2: Table Of Contents
™ ® 1. Flexcell Flex Jr. Tension System Components ............1 ™ ® 2. Setup of the Flexcell Flex Jr. Tension System ............1 2.1 Packing List ........................1 ™ 2.2. Location of the Flex Jr. Tension System ............... 1 ™... -
Page 3: One Year Limited Parts Warranty
If the original shipping box is not available, Flexcell will send the required protective shipping container. (Flexcell will recommend the insurance value for parts or equipment to be shipped.) Return carrier shipping costs within the US and Canada will be paid by Flexcell from the service center. - Page 4 Congratulations on your purchase of the Flexcell Dynamic Culture System: Flex Jr. Tension ® system. This system comes with a one (1) year warranty on the hardware manufactured by Flexcell ™ ® International Corporation. Please do not open the sealed Flex Jr.
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Page 5: Federal Communications Commission Radio Frequency Interference Statement
® LEXCELL NTERNATIONAL ORPORATION FEDERAL COMMUNICATIONS COMMISSION RADIO FREQUENCY INTERFERENCE STATEMENT This computing equipment has been type-tested and found to comply with the limits for a Class B digital device in accordance with the specifications for Subpart J of Part 15 of FCC Rules. These limits are designed to provide reasonable protection against harmful radio and TV interference in a residential installation. -
Page 6: Flex Jr. ™ Quick Start Instructions
® LEXCELL NTERNATIONAL ORPORATION Flex Jr. UICK TART NSTRUCTIONS ™ 1. Choose a lab location for the Flex Jr. the provided quick disconnect to the open System that is near, and adjacent to the end of the tubing. incubator in the cell culture area. NOTE: The overall length of the FLEX OUT tubing should not exceed 10’... - Page 7 ® LEXCELL NTERNATIONAL ORPORATION 18. Turn ON the vacuum system. Be sure that the vacuum level is at -90 kPa. Click on Start to run the regimen. The plate should begin flexing. Make a visual check to verify membrane movement. 19.
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Page 8: Flexcell Flex Jr. Tension System Components
2009 Flexcell International FLEXCELL, BioFlex, FlexLink and FlexSoft are trademarks of Flexcell International Corporation. MS Windows and MS Office are trademarks of the Microsoft Corporation. Adobe Acrobat is a trademark of Adobe Systems Inc. Dell OptiPlex is a trademark of Dell. -
Page 9: Tension Flexlink
® LEXCELL NTERNATIONAL ORPORATION Figure 1. Flexcell Flex Jr. Tension System. ® ™ 1) Surge protected power strip, 2) power outlet, 3) Ethernet cable, 4) reinforced vacuum tubing, 5) compressed air regulator/filter, 6) ” VENT tubing, 7) ” SYSTEM tubing, 8) ”... -
Page 10: Connecting The System Vacuum Lines
Prior to storing, be sure to run a drying regimen before inserting the plugs (see section 3.16). Figure 4. Flex Jr.TM FlexLink rear view. ® A) White plugs with red securing clips in the ports. -
Page 11: Connection To Microscopy Devices
ONNECTION ICROSCOPY ® FlexLink is recommended. EVICES ® Flexcell International Corp. recommends Use the tubing adapter as shown in Figure 4 to the Leybold Trivac D8B Pump vacuum ™ connect the Flex Jr. to the microscope pump (includes the D8B pump system, device. -
Page 12: Flexsoft Flex Jr
All tubing ® connections made to the first FlexLink ® NOTE: Flexcell recommends that the power should be made exactly the same with the ® saving or screen saver features in the Windows ®... -
Page 13: Operation Instructions
® done when using one FlexLink 3. OPERATION INSTRUCTIONS ® DISCLAIMER: Although the Flexcell System Turn ON the power switch on the computer ™ can be programmed to simulate most of the strain ®... -
Page 14: Main Display Window
0.01%. Therefore, the signal to noise ratio for the combined ® Flexcell system of circuits, hardware and transducers is high, lending confidence in the displayed plot. Nevertheless, the user can select a data smoothing option that is a simple weighted average of the points. -
Page 15: Configuring Users
® LEXCELL NTERNATIONAL ORPORATION coordinates for the waveform. These are arrow buttons at either end of the axis. The displayed when the left mouse button is button with arrows pointing away from each clicked and held down in the graph area. other increases the axis scale, while the When this is done, a pair of crosshairs appears button with arrows pointing toward each... -
Page 16: Creating Regimens
® LEXCELL NTERNATIONAL ORPORATION and click on Add User. The new name should 1) Type a regimen name in the box labeled appear in the Existing Users box. Regimen Name. To delete a user, click on the appropriate user 2) Choose the desired waveform shape name in the Existing Users box so that it is under Shape by clicking on the highlighted in blue and then click on Delete... -
Page 17: Creating A Regimen With A Custom Waveform Shape
® LEXCELL NTERNATIONAL ORPORATION step number in the box under Back To. 13) To delete a regimen, simply double-click Then, type the number of times to repeat on the desired regimen name in the this process in the box under Repeat. Regimens for Selected User box, and then click on Delete Regimen. -
Page 18: Assigning Regimens
® LEXCELL NTERNATIONAL ORPORATION ™ In the Regimen menu of the Flex Jr. duration, this value will be MAXIMUM, software, select Create new. A new window and the regimen cannot be saved. Adjust will appear entitled Regimens with a list of the time and/or cycle number to decrease the existing users and their regimens. -
Page 19: Starting Regimens
® LEXCELL NTERNATIONAL ORPORATION waveforms (e.g. square waves), the idealized pattern may appear to have some unusual elements (e.g. rounded corners). The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure (e.g. rising/falling edge of a square wave). -
Page 20: Determine The Progress Of The Regimen
4.0 Mb data file. To turn continuously. The displays for Run Time, ® this feature OFF, please contact Flexcell Step number, Cycle number, Jump to number, and Min and Max % elongations will To see the stored data, in the Data Analysis be updated in the upper right hand corner of menu, select Data Viewer. -
Page 21: Shutting Down The System
® LEXCELL NTERNATIONAL ORPORATION The x-axis scale allows for a time interval to to remove any moisture entrained into the be set between 0.1 and 1000 seconds. Click on valves during normal operation. This will one of the two small double-arrow buttons at greatly prolong the life of the valves and either end of the axis to change this scale. -
Page 22: Exiting The Flex J Rprogram
See Tech ™ To exit the Flex Jr. software, in the Report 200: Use of Water Trap and Drying Controllers menu, select Exit. ® Filter with the Flexcell Tension Systems for instructions on regenerating the desiccant beads. -
Page 23: Addendums To The Flex Jr
® LEXCELL NTERNATIONAL ORPORATION 4. ADDENDUMS TO THE FLEX JR. TENSION SYSTEM ™ PTIMAL ERFORMANCE OF THE incubator for any condensed moisture ENSION UNIT (Fig. 14). If water is visible in the tubing, it must be removed before running ® another experiment. -
Page 24: System Troubleshooting
If air is cycling at the ® defined below, please contact Flexcell before end of the tubing then the problem is with attempting any repairs. - Page 25 See the true, close and restart the software. Try solution to Problem 1 to correct this. Be sure downloading the regimen again. If the ® problem is not resolved, call Flexcell to empty the Water Trap frequently if this further instructions...
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Page 26: Waveform Shape Definitions
® LEXCELL NTERNATIONAL ORPORATION 6. WAVEFORM SHAPE DEFINITIONS ½ Sine Wave Sine Wave This waveform provides a smooth, physiological This waveform provides a smooth, physiological variation in pressure at the max% with a quick variation in pressure from min% to max%. transition at the min%. -
Page 27: Flexcell
This waveform applies a pressure pattern that mimics the shape as seen on an electrocardiogram the actual pressure wave generated by a heartbeat. Static Wave This waveform applies a constant state of pressure. 7. FLEXCELL FLEX JR. TENSION SYSTEM SPECIFICATIONS ® ™... - Page 28 ® Train culture plates. ™ ENSION OMPONENTS AND PECIFICATIONS • ™ ® FlexSoft Flex Jr. V2.0 (Flexcell Flex Jr. Software) and Microsoft Windows 10 • ™ Flex Jr. Tension FlexLink controller • ® Flexcell BioFlex single plate baseplate •...
- Page 29 ® LEXCELL NTERNATIONAL ORPORATION Maximum allowable transducer inaccuracy: +/-1.0 kPa BioFlex 25mm Loading Station Static Performance (high side transducers): Maximum allowable waveform variance from static line: +/- 0.25% elongation Maximum % elongation error (actual vs. programmed): +/-0.5% elongation Maximum allowable transducer inaccuracy: +/-2.0 kPa BioFlex 25mm Loading Station Dynamic Performance (high side transducers): Maximum % elongation error (actual vs.
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Page 30: Recommended Maintenance For The Flex Jr
® LEXCELL NTERNATIONAL ORPORATION Weight (unit only): 11.8 lbs. (5.35 kg) ™ ENSION ACUUM EQUIREMENTS To achieve the maximum system capability, the minimum vacuum source requirements are: • Maximum Vacuum: : -100 kPa • Free Airflow Rate: 5.7 cfm (161 L/min) 8. - Page 31 This manual is believed to ® be complete and accurate at the time of publication. In no event shall Flexcell International Corporation be liable for incidental or consequential damages in connection with or arising from ™...