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flexcell FX-5000 User Manual

Compression system
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USER MANUAL
Flexcell
Compression System
Culturing Cells in a Mechanically Active Environment
Flexcell International Corporation  2730 Tucker Street, Suite 200  Burlington, NC 27215
800-728-3714  (919) 732-1591  FAX: (919) 732-5196  www.flexcellint.com
COPYRIGHT © 2009 FLEXCELL
FX-5000
®
06-20-17
Rev 16.0
INTERNATIONAL CORPORATION
™
™

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  • Page 1 06-20-17 Rev 16.0 Culturing Cells in a Mechanically Active Environment ™ Flexcell International Corporation  2730 Tucker Street, Suite 200  Burlington, NC 27215 800-728-3714  (919) 732-1591  FAX: (919) 732-5196  www.flexcellint.com  COPYRIGHT © 2009 FLEXCELL INTERNATIONAL CORPORATION...

  • Page 2: Table Of Contents

    ® ™ 2. Setup of the Flexcell FX-5000 Compression System ........1 2.1 Packing List ........................1 ™ 2.2. Location of the FX-5000 Compression System ............1 ™ ® 2.3 Setup of the Computer and FX5K Compression FlexLink .......... 2 2.4 Incubator Access Ports ......................

  • Page 3: One Year Limited Parts Warranty

    If the original shipping box is not available, Flexcell will send the required protective shipping container. (Flexcell will recommend the insurance value for parts or equipment to be shipped.) Return carrier shipping costs within the US and Canada will be paid by Flexcell from the service center.

  • Page 4: Dell Computer Warranty Information

      Dear Customer: Congratulations on your purchase of the Flexcell FX-5000 system. This system comes with a one (1) year warranty. The Dell computer that is included with this equipment will be transferred into the Principal Investigator and University’s name at the time of purchase. This computer comes with an excellent warranty through Dell.

  • Page 5: Federal Communications Commission Radio Frequency Interference Statement

    ® LEXCELL NTERNATIONAL ORPORATION FEDERAL COMMUNICATIONS COMMISSION RADIO FREQUENCY INTERFERENCE STATEMENT This computing equipment has been type-tested and found to comply with the limits for a Class B digital device in accordance with the specifications for Subpart J of Part 15 of FCC Rules. These limits are designed to provide reasonable protection against harmful radio and TV interference in a residential installation.

  • Page 6: Fx-5000 ™ Quick Start Instructions

    ORPORATION  FX-5000 UICK TART NSTRUCTIONS ™ 1. Choose a location for the FX-5000 plate lids during insertion. Place the  Compression system that is near the Plexiglas window into the clamping system, incubator. evenly along the bottom four clamping bars.
  • Page 7 ® LEXCELL NTERNATIONAL ORPORATION 16. In Controllers menu, select the appropriate satisfied that the regimen is correct, click on ® FlexLink number to which the regimen will Stop. be downloaded. 19. Turn ON the pressure source. **Do not 17. In the Regimen menu, select Assign. Choose allow the input pressure to exceed 20 psi or the proper platform, user, and regimen the transducers may be damaged.

  • Page 8: Flexcell ® Fx-5000 ™ Compression System Components

    2009 Flexcell International FLEXCELL, FlexLink and FlexSoft are trademarks of Flexcell International Corporation. MS Windows and MS Office are trademarks of the Microsoft Corporation. Adobe Acrobat is a trademark of Adobe Systems Inc. Dell OptiPlex is a trademark of Dell.

  • Page 9: Setup Of The Computer And Fx5K ™ Compression Flexlink

    48.37 lbs (21.96 kg). to ensure of the proper length. NOTE: The PUMP CONTROL CONNECTION (Fig. 2) feature is only available with the FX- ™ 5000 Tension system. Figure 1. Flexcell FX-5000 Compression ® ™ System Figure 3. Dell OptiPlex rear view 2.3 S...

  • Page 10: Incubator Access Ports

    ® LEXCELL NTERNATIONAL ORPORATION Then, connect the hub to the Ethernet port on representative should open the incubator the back of the computer. access ports. Connect the system tubing as follows: Once the access port has been located, insert 1. Connect the ⅜” (9.5 mm) O.D. blue tubing the FLEX IN and FLEX OUT tubing from the pressure source to the SYSTEM through the access port into the incubator.

  • Page 11: Sample Preparation And Setup Of Bio Press Culture Plates And Baseplate Clamping Assembly

    ® LEXCELL NTERNATIONAL ORPORATION 2.5 S To prepare a sample for loading, remove the AMPLE REPARATION AND ETUP sample holder from the plate using a pair of ™ RESS ULTURE LATES sterile forceps. Place the tissue or gel sample ASEPLATE LAMPING into the hole at the center of the removable SSEMBLY...
  • Page 12 ® LEXCELL NTERNATIONAL ORPORATION where x is the # of 180 degree clockwise turns withstand high pressures. Never operate the compression baseplate without this window in required for the center screw to touch the place. sample and h is the sample height in millimeters.

  • Page 13: Connecting The System Positive Pressure Lines

    ® the positive pressure source. Contact Flexcell NOTE: If using less than 4 plates in an for information on purchasing a pressure experiment, use an old plate or support in place of reservoir.

  • Page 14: Flex Soft ® Fx-5000 Software And System Power - Up

    ® load Windows . Select and double click on Combinations of high frequencies and forces may ™ the FX-5000 icon. not be achievable due to the high pressure level in the short period of time requested. An example ® NOTE: Flexcell...

  • Page 15: Using Additional Flexlinks

    The capability of the pressure source with ® more than one FlexLink will need to be tested experimentally with the particular ® setup. FlexLinks must be placed side-by- Flexcell International Corp. www.flexcellint.com side during use. Regimen #1 Regimen: READY downloaded User name ®...

  • Page 16: Operation Instructions

    ™ 5000 icon (Fig. 11) on the desktop to start ® ™ the FlexSoft FX-5000 software. A main display window will appear (see Fig. 13). Figure 12. FX-5000 tutorial ™ Figure 11. FX-5000 icon ™ 3.2 U FX-5000 ™ SING THE...
  • Page 17 Plot Units the pressure plot as a function of voltage from section as the minimum and maximum ™ the FX-5000 Compression System pressure points at which the membrane stretches with transducer, the rough data yield a plot with a each cycle.

  • Page 18: Configuring Users

    To the right of the plot is a set of command buttons which allow the user to: 1) Start a regimen. 2) Stop a regimen. Figure 14. FX-5000 user editor ™ 3) Pause a regimen. 3.5 C 4) Resume a paused regimen.
  • Page 19 ® LEXCELL NTERNATIONAL ORPORATION creating a new regimen. Alternatively, follow regimen step, enter the return step the sequence of steps below: number in the box under Back To. Then, type the number of times to repeat this 1) Type a regimen name in the box labeled process in the box under Repeat.

  • Page 20: Creating A Regimen With A Custom Waveform Shape

    13) To delete a regimen, simply double-click ™ on the desired regimen name in the In the Regimen menu of the FX-5000 Regimens for Selected User box, and software, select Create new. A new window then click on Delete Regimen. Confirm will appear entitled Regimens with a list of the deletion by clicking on Yes.

  • Page 21: Assigning Regimens

    ® ™ For the Flexcell FX-5000 Compression continuously. The displays for Run Time, System, the BioPress and StagePresser platforms Step #, Cycle #, Jump #, and Min and Max will be used depending on the purchased device.

  • Page 22: Pausing Regimens

    ™ as generated by the FX-5000 software, and step. Stopping is different from pausing, in is the actual waveform pattern sent to the that pausing leaves the regimen at its current ®...

  • Page 23: Data Analysis

    Sample Diameter (mm) box. Click Calculate. The calculated pressure in kPA will appear in the Pressure (kPa) box. To exit this window, click on Close. Figure 18. FX-5000 data display ™ 3.16 D The y-Axis control box allows for automatic NALYSIS or manual control of the scale on the y-axis.

  • Page 24: Exiting The Fx-5000 Program

    ROGRAM pressure over the designated time period (in seconds, range of 0.1-1000 seconds) shown in ™ To exit the FX-5000 software, in the the Time (s) box. Controllers menu, select Exit. 4. SYSTEM TROUBLESHOOTING ™...
  • Page 25 LEXCELL NTERNATIONAL ORPORATION ® ® regimen inputs, check your input pressure FlexLink . Contact Flexcell International level from your pressure source. Try for further instructions. increasing it slightly. If the problem remains ® Problem 3: Regimen wave is fluttering or then the FlexLink may need recalibration.

  • Page 26: Application Notes

    Try Solution 6: downloading the regimen again. If the This problem is usually due to a lack of ® problem is not resolved, call Flexcell communication between the computer and further instructions. ® ™...

  • Page 27: Waveform Shape Definitions

    70% ethanol, but be sure tubing is completely After cleaning and disinfecting, seal  baseplate and gasket in a plastic bag. Store dry before connecting to the Flexcell Unit. baseplate flat and be sure that the entire Contact time (time surface remains wet) of the...
  • Page 28 ® LEXCELL NTERNATIONAL ORPORATION Triangle Wave Square Wave This waveform provides a linear-ramped rise to the This waveform provides the fastest possible rise to max% and a linear-ramped fall back to the min%. max%, followed by the fastest possible fall to min%. Heart (E) Wave Heart (P) Wave This waveform applies a pressure pattern that mimics...

  • Page 29: Flexcell Fx-5000 Compression System Specifications

    ® LEXCELL NTERNATIONAL ORPORATION 7. FLEXCELL FX-5000 COMPRESSION SYSTEM ® ™ SPECIFICATIONS FX-5000 ™ OMPRESSION YSTEM EATURES  Uses air pressure to deform a flexible-bottom culture plate yielding up to 15 pounds of applied force to a tissue or 3D cell culture sample ...
  • Page 30 If a house pressure system is being used and there are no means to test the flow rate capabilities, run the ™ house pressure system with the FX-5000 to determine if it is able to maintain 12-15 PSI (83-104kPa) consistently while the FlexLink ®...

  • Page 31: Appendix: Fx-5000

    This manual is believed to ® be complete and accurate at the time of publication. In no event shall Flexcell International Corporation be liable for incidental or consequential damages in connection with or arising from ™...

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