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USER MANUAL
Flexcell
Tension System
Culturing Cells in a Mechanically Active Environment
Flexcell International Corporation 2730 Tucker Street, Suite 200 Burlington, NC 27215
800-728-3714 (919) 732-1591 FAX: (919) 732-5196 www.flexcellint.com
COPYRIGHT © 2009 FLEXCELL
FX-5000
®
06-14-17
Rev 21.0
INTERNATIONAL CORPORATION
™
™
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Summary of Contents for flexcell FX-5000 Tension System
- Page 1 06-14-17 Rev 21.0 Culturing Cells in a Mechanically Active Environment ™ Flexcell International Corporation 2730 Tucker Street, Suite 200 Burlington, NC 27215 800-728-3714 (919) 732-1591 FAX: (919) 732-5196 www.flexcellint.com COPYRIGHT © 2009 FLEXCELL INTERNATIONAL CORPORATION...
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Page 2: Table Of Contents
™ FX-5000 Quick Start Instructions ....................v ® ™ 1. Flexcell FX-5000 Tension System Components ............ 1 2. Setup of the Flexcell ® FX-5000 ™ Tension System ............. 1 2.1 Packing List ........................1 ™ 2.2. Location of the FX-5000 Tension System .............. -
Page 3: One Year Limited Parts Warranty
If the original shipping box is not available, Flexcell will send the required protective shipping container. (Flexcell will recommend the insurance value for parts or equipment to be shipped.) Return carrier shipping costs within the US and Canada will be paid by Flexcell from the service center. -
Page 4: Dell Computer Warranty Information
Dear Customer: Congratulations on your purchase of the Flexcell FX-5000 system. This system comes with a one (1) year warranty. The Dell computer that is included with this equipment will be transferred into the Principal Investigator and University’s name at the time of purchase. This computer comes with an excellent warranty through Dell. -
Page 5: Federal Communications Commission Radio Frequency Interference Statement
® LEXCELL NTERNATIONAL ORPORATION FEDERAL COMMUNICATIONS COMMISSION RADIO FREQUENCY INTERFERENCE STATEMENT This computing equipment has been type-tested and found to comply with the limits for a Class B digital device in accordance with the specifications for Subpart J of Part 15 of FCC Rules. These limits are designed to provide reasonable protection against harmful radio and TV interference in a residential installation. -
Page 6: Fx-5000 ™ Quick Start Instructions
® LEXCELL NTERNATIONAL ORPORATION FX-5000 UICK TART NSTRUCTIONS ™ 1. Choose a location for the FX-5000 Tension b. Connect the quick disconnects for the ¼” system that is near the incubator. (6.4 mm) and ⅜” (9.5 mm) tubing to the baseplate(s). -
Page 7: Flexcell ® Fx-5000 ™ Tension System Components
2009 Flexcell International FLEXCELL, BioFlex, FlexLink and FlexSoft are trademarks of Flexcell International Corporation. MS Windows and MS Office are trademarks of the Microsoft Corporation. Adobe Acrobat is a trademark of Adobe Systems Inc. Dell OptiPlex is a trademark of Dell. -
Page 8: Setup Of The Computer And Fx5K ™ Tension Flexlink
Figure 3. Dell OptiPlex rear view 2.3 S ETUP OF THE OMPUTER FX5K ™ ® ENSION Figure 1. Flexcell FX-5000 Tension System ® ™ Setup the Dell OptiPlex desktop computer peripherals according manufacturer’s instructions. -
Page 9: Incubator Access Ports
® LEXCELL NTERNATIONAL ORPORATION ® storing the FlexLink for an extended period 4. Connect a Water Traps inline with the of time. Prior to storing, be sure to run a clear ⅜" tubing and the blue ¼” tubing. drying regimen before inserting the plugs (see See the Addendum (pg 16, "B") for section 3.17) further details concerning the placement... -
Page 10: Setup Of The Bioflex ® Baseplate(S) With Loading Stations ™ And Culture Plates
® LEXCELL NTERNATIONAL ORPORATION are to be used simultaneously, connect the baseplates using the configuration as shown in 2. If using Loading Stations ™ , place the Figure 6. four sets of Loading Stations ™ into the baseplate wells and apply silicone grease NOTE: Please see the Appendix (pg. -
Page 11: Flex Soft ® Fx-5000 Software And System Power - Up
The vacuum pump and pressure reservoir can be purchased directly from ® Flexcell International Corp. NOTE: Even with an optimal vacuum source, Figure 7. Flexcell FX-5000 Tension System ® ™ power set-up with auto control pump feature there will still be some system limitations due to airflow. -
Page 12: Lcd Display On The Fx5K ™ Tension Flexlink
ON the power switches at the computer, simulated Regimen Name ® monitor and FX5K ™ Tension FlexLink . The Flexcell International Corp. ® system should then boot and load Windows www.flexcellint.com ™ Select and double click on the FX-5000 icon. Regimen has... -
Page 13: Operation Instructions
% elongation the screen. The tutorial explains the main ® values. To increase performance, Flexcell steps involved in configuring users, creating recommends shortening the tubing length as much regimens, and starting regimens. The tutorial... - Page 14 Figure 9. FX-5000 tutorial ™ ® Flexcell system of circuits, hardware and transducers is high, lending confidence in the displayed plot. Nevertheless, the user can select a data smoothing option that is a simple weighted average of the points.
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Page 15: Configuring Users
® LEXCELL NTERNATIONAL ORPORATION elapsed regimen time, and the one on the right 3) Pause a regimen. displays the remaining regimen time, using 4) Resume a paused regimen. following format: dd:hh:mm:ss 5) Simulate a regimen. (days:hours:minutes:seconds). 6) Reset the controller. The block at the top right contains the current 7) Control the vacuum pump. -
Page 16: Creating Regimens
® LEXCELL NTERNATIONAL ORPORATION 3.5 C 3) Enter desired minimum REATING EGIMENS maximum % elongation for the plate membranes (0-30%) in the boxes under NOTE: Please see the Appendix (pg. 25) for the maximum allowable % elongations when using Min and Max. two baseplates simultaneously. -
Page 17: Creating A Regimen With A Custom Waveform Shape
® LEXCELL NTERNATIONAL ORPORATION 3.6 C 9) When the regimen step is complete, click REATING A EGIMEN WITH A on Add Step at the bottom. Every time a USTOM AVEFORM HAPE new/edited step is saved, the overall duration of the regimen will be calculated In the top row of an Excel spreadsheet, enter and displayed in the box labeled Duration the % elongation values for the desired... -
Page 18: Assigning Regimens
® LEXCELL NTERNATIONAL ORPORATION 3.7 A To create a new regimen, click on Run SSIGNING EGIMENS Wizard in the bottom left hand corner. This option will bring up a wizard to create a new In the Controllers menu select the regimen. -
Page 19: Starting Regimens
® LEXCELL NTERNATIONAL ORPORATION not be able to run. In which case, click Reset for a period of time (e.g. dampened sine ® in the FlexLink window, and then assign the wave during beginning of plateau in a square desired regimen again. waveform). -
Page 20: Resetting The Flexlink
Mb data file. To turn this feature OFF, please up for this option to work, and the ON/OFF switch ® contact Flexcell on the vacuum pump must be in the ON position. To see the stored data, in the Data Analysis For manual control of the pump through the menu, select Data Viewer. -
Page 21: Shutting Down The System
® LEXCELL NTERNATIONAL ORPORATION seconds, range of 0.1-1000 seconds) shown in the Time (s) box. The Start time box can be set so that the data viewed in the plot begin at the specified time entered here. Use the timeline at the top of the graph to navigate through the regimen. -
Page 22: Exiting The Fx-5000 Program
® LEXCELL NTERNATIONAL ORPORATION Connect the blue polyethylene ” OD tubing Frequency: 2.0 from the drying filter to the FLEX OUT port DC %: 50.0 ® on the back of the FlexLink . Assign the Cycles: 21600 Drying regimen under the Shutdown user, dd:hh:mm:ss: 0:02:00:10 using the BioFlex Plate, no Loading Stations platform. -
Page 23: System Troubleshooting
If experiencing symptom a, there is likely a properly and the solution is not clearly outlined or leak or crimped tubing somewhere in the ® defined below, please contact Flexcell before system. First, check all tubing to be sure that attempting any repairs. - Page 24 If the regimen wave is flatlining but the the inputs, the problem is likely not a leak plates are still cycling air pressure, try the ® but calibration. Call Flexcell for further following: instructions. If the output max % elongation increases significantly when the quick Disconnect the blue 1/4"...
- Page 25 If the the growth surface. Also, check with ® assign function sees that a user has nothing to Flexcell to be sure that the best matrix assign an error is displayed. Simply delete the coating for attachment of the particular cell user(s) without a regimen or make and save a type is being used.
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Page 26: Application Notes
® LEXCELL NTERNATIONAL ORPORATION ® true, close and restart the software. Try problem is not resolved, call Flexcell downloading the regimen again. If the further instructions. 6. APPLICATION NOTES The above decontamination procedure ASEPLATE AND ASKET should be performed before and after each... -
Page 27: Waveform Shape Definitions
® LEXCELL NTERNATIONAL ORPORATION 7. WAVEFORM SHAPE DEFINITIONS ½ Sine Wave Sine Wave This waveform provides a smooth, This waveform provides a smooth, physiological variation in pressure at the physiological variation in pressure from max% with a quick transition at the min%. min% to max%. -
Page 28: Flexcell ® Fx-5000 ™ Tension System Specifications
® LEXCELL NTERNATIONAL ORPORATION Static Wave This waveform applies a constant state of pressure. 8. FLEXCELL FX-5000 TENSION SYSTEM SPECIFICATIONS ® ™ FX-5000 ™ ENSION YSTEM EATURES Uses vacuum to deform a flexible-bottom culture plate yielding up to 33% substrate elongation ... - Page 29 ® ISSUE RAIN CCESSORY PTIONAL (Note: If purchased as a system, the Tissue Train ® Accessory Kit is substituted for the Flexcell ® vacuum pressure baseplate, gaskets, and acrylic window for BioFlex ® culture plates and BioFlex ® Loading Stations ™...
- Page 30 ® LEXCELL NTERNATIONAL ORPORATION ™ OADING TATIONS PECIFICATIONS 25mm Diameter Loading Stations ™ (equibiaxial strain): Minimum achievable % elongation with the FX-5000 ™ : 0.8% Maximum achievable % elongation with the FX-5000 ™ : 21.8% ™ 28mm Diameter Loading Stations (equibiaxial strain): ™...
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Page 31: Appendix: Fx-5000 ™ Tension System Dual Baseplate Operation
This manual is believed to ® be complete and accurate at the time of publication. In no event shall Flexcell International Corporation be liable for incidental or consequential damages in connection with or arising from ™...