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USER MANUAL
Streamer
System
®
STR-4000
05-12-17
Rev 6.0
™
Culturing Cells in a Mechanically Active Environment
Flexcell International Corporation 2730 Tucker Street, Suite 200 Burlington, NC 27215
800-728-3714 (919) 732-1591 FAX: (919) 732-5196 www.flexcellint.com
COPYRIGHT © 2003 FLEXCELL
INTERNATIONAL CORPORATION
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Related Manuals for flexcell Streamer System STR-4000
Summary of Contents for flexcell Streamer System STR-4000
- Page 1 05-12-17 Rev 6.0 ™ Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street, Suite 200 Burlington, NC 27215 800-728-3714 (919) 732-1591 FAX: (919) 732-5196 www.flexcellint.com COPYRIGHT © 2003 FLEXCELL INTERNATIONAL CORPORATION...
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Page 2: Table Of Contents
Filling the System to Eliminate Air Bubbles .................. 21 Post-Experimental Analysis ......................21 Application Notes ......................22 ® Culturing Cells on Culture Slips ....................22 ® Appendix: Parallel Streamer Shear Stress Numbers ........... 23 Warranty Information ..................... 24 Contacting Flexcell ......................25... -
Page 3: Getting Started
® LEXCELL NTERNATIONAL ORPORATION 1. GETTING STARTED production of secreted molecules into the NTRODUCTION perfusate. Fluid-induced shear stress occurs in every tissue in the body as a result of interstitial fluid movement. Tissue deformation compression, tension or shear forces results in the movement of interstitial fluid around cells. -
Page 4: Sterilizing The Streamer
® LEXCELL NTERNATIONAL ORPORATION 4. Connect the second pulse dampener to the ® inlet port (bottom) of the Streamer with one of the 2’ (0.6 m) pieces of silicone tubing. 5. Connect the outlet port (top) of the Streamer to the quick disconnect on the ®... -
Page 5: Streamer ® Placement In The Incubator
® LEXCELL NTERNATIONAL ORPORATION alcohol can also be pumped through the system Once this is set, the display on the pump for cleaning, however, this will not completely will read the flow rate for that particular sterilize the system. tubing as the pump is running. Consult the appendix or the data following this manual ®... - Page 6 ® LEXCELL NTERNATIONAL ORPORATION Regime and Hardware, then click Update. system. Do the same with the second pulse The regimen is now ready to start. dampener. Once this process is complete, ® 7. Culture cells on six Culture Slips . Be sure allow flow to continue and go to the next ®...
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Page 7: Streamsoft ™ V4.2 Software
See the instructional video, Streamer® ® 23. Click the Start button in the software (or set Assembly, Flexcell ’s website the pump to the desired flowrate and (http://www.flexcellint.com/videos- depress the start button). Your regimen will instruct.htm) for a demonstration of how to... -
Page 8: Main Panel
® LEXCELL NTERNATIONAL ORPORATION and Configure System Variables, pages 15- ensure accurate flow results for your system. For instructions on setting up these 17. Complete this setup before proceeding parameters, see Configure Testing Apparatus with any experiments. MAIN PANEL ENERAL NFORMATION Function: The default main panel allows the user to verify that the system is running and to stop the tests at any time. -
Page 9: System Tab
® LEXCELL NTERNATIONAL ORPORATION YSTEM Function: This panel is used to run the experiments. Each System tab is identical. Buttons and Fields User Current user in currently configured regime Regime Regime currently configured ® ™ Apparatus Device being used with the software (Streamer or FlexFlow Data File Name of file to which data is being saved (if appropriate) Configure (load) the experiment. -
Page 10: Pre-Test Configuration
® LEXCELL NTERNATIONAL ORPORATION ONFIGURATION Function: This panel allows the user to configure the parameters of an experiment. It appears when the user presses the Configure button on the System panel. The information selected here is transferred to the User, Regime and Apparatus fields on the System panel. Buttons and Fields Users List of all users. -
Page 11: Pull-Down Menus
® LEXCELL NTERNATIONAL ORPORATION ENUS This section summarizes the function of each item in the three pull-down menus. File Operate Help -Print -Manual Mode -Help -Exit -View Data -About LabVIEW -Users -Configure Regime -Configure Apparatus -Configure System -Reinitialize Hardware File Print Allows user to print a copy of the current panel. -
Page 12: Operate Menu
® LEXCELL NTERNATIONAL ORPORATION OPERATE MENU ANUAL Function: This panel allows the user to manually control the pump. The actual flow rate and speed of the pump (RPM) are shown on the graph when the pump is working. Manual mode may be used to troubleshoot the pump operation. -
Page 13: View Data
® LEXCELL NTERNATIONAL ORPORATION Function: This panel allows the user to view previously collected experimental data in a table format. Buttons and Fields File The complete file path to the data file being viewed Table Contents of the experimental data log file Export File Export data to a spreadsheet-compatible format Print a copy of this panel to the Windows default printer or write a copy Print... -
Page 14: Configure Users
® LEXCELL NTERNATIONAL ORPORATION ONFIGURE SERS Function: This panel allows the user to create or delete users. Buttons and Fields Existing Users Lists all current users of the system User Name Field used to enter new users Add User Add new users to the system. Delete User Delete users from the system. -
Page 15: Configure Regimes: Setup Parameters
® LEXCELL NTERNATIONAL ORPORATION ONFIGURE EGIMES ETUP ARAMETERS Function: This panel allows the user to configure (create) a regime. Buttons and Fields Existing Users List of all users; select a user from the list using the mouse. Regimes for List of regimens created by the current user. Selecting from this list will load that regimen and allow the user to view and/or modify that regime. - Page 16 ® LEXCELL NTERNATIONAL ORPORATION Specifies the direction or type of flow for this step (forward, reverse, Flow Type pulsed (square wave), oscillation) When using pulsed (square wave) or oscillatory flow, specifies how long the valves remain in a position to allow the fluid flow to continue ON/HI (s) unhindered or flow in the forward direction, respectively.
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Page 17: Configure Testing Apparatus
® LEXCELL NTERNATIONAL ORPORATION ONFIGURE ESTING PPARATUS ® Function: This panel allows the user to create, modify or delete a testing apparatus (Streamer FlexFlow flow chamber). As each Streamer ® and FlexFlow device is manufactured to strict dimensional specifications, the values for the height and width of the chambers must be entered into the software for each individual device. -
Page 18: How To Enter The Proper Values For Your Device
® LEXCELL NTERNATIONAL ORPORATION ® Height of the flow area (cm) in a single chamber of the Streamer FlexFlow device. This number is found in the back of the manual for your device listed as Flow Area Height (cm). Viscosity of the perfusate/media used in the experiment. -
Page 19: Configure System Variables
® LEXCELL NTERNATIONAL ORPORATION ONFIGURE YSTEM ARIABLES Function: This panel is used to select three system parameters – Communications port, data saving, and the presence of valves in the flow system. Buttons and Fields Com 1 is the default port. This should be changed only if there is a Pump Serial Port conflict with this port on your computer. -
Page 20: Reinitialize Hardware
® LEXCELL NTERNATIONAL ORPORATION EINITIALIZE ARDWARE Function: This panel will appear when the computer program is first started. It will also appear when the Reinitialize Hardware item is selected in the Operate menu. When the system is properly initiated, the pump will display PO1. If power to the pump is cycled during experimentation, or communication is lost, the user should reinitialize the hardware before turning off the program and starting it again. -
Page 21: Doing An Experiment
® LEXCELL NTERNATIONAL ORPORATION 3. DOING AN EXPERIMENT 5. Click on Save Regime. The regime name VERVIEW should appear in the Regimes for Selected Users field at the top. There are two major components to running an 6. Specify the Flow Type (FWD, REV, ®... -
Page 22: Setting Up An Experiment
® LEXCELL NTERNATIONAL ORPORATION tubing size and pump assigned to it. Modify is going from the vertex of the angle to the shear stresses if necessary. open end of the angle. Leave the pulse 4. The regime is now ready to run. dampener in this position until the fluid comes through the outlet fitting again, then lay the pulse dampener down horizontally. -
Page 23: Filling The System To Eliminate Air Bubbles
® LEXCELL NTERNATIONAL ORPORATION end. Be careful not to stimulate or crush any 18. If you are running the experiment manually, cells on the slide. set the pump to the desired flow rate and ® 13. Place the Culture Slip into one of the slots press the start button. -
Page 24: Application Notes
® LEXCELL NTERNATIONAL ORPORATION Cells can be lysed with appropriate buffer subsequent collection cell supernatant. The medium can then be to collect total RNA or intracellular assayed for released effector molecules. proteins. The cells can be trypsinized for replating or ... -
Page 25: Appendix: Parallel Streamer Shear Stress Numbers
® LEXCELL NTERNATIONAL ORPORATION APPENDIX: PARALLEL STREAMER SHEAR STRESS ® NUMBERS Flow Area Height(cm) Flow Area Width(cm) 0.0513 2.3396 Serial # Flow Factor SGS-1109 System Flow Rate (ml/min) Shear Stress (dyn/cm^2) 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0 21.0... -
Page 26: Warranty Information
Parts must be properly packed in original container and shipped to our factory service center or distributor with all shipping costs prepaid if the unit is out of warranty coverage. If the original shipping box is not available, Flexcell will send the required protective shipping container. -
Page 27: Contacting Flexcell
Corporation assumes no responsibility for any errors that may appear in this guide. This manual is believed to be complete and accurate at the time of publication. In no event shall Flexcell International Corporation be liable for incidental or consequential damages in connection with or arising from the...
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