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Leica SP 5 II HCA Quick Manual page 3

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6) Now you can start the software LAS-AF and insert your sample into
the sample holder. As the start-up routine moves the stage, it is not
recommended to focus into your sample until after the software has
fully started.
7) Choose either machine – with pump or machine – without pump
according to your needs. Usually the pump is ever only used with wellplates
when 63x water immersion objective is needed. Start the software from the
OK button.

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