Germfree BBF 2 User Manual

Germfree BBF 2 User Manual

Class ii, type a2 biosafety cabinet
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Class II, Type A2 Biosafety Cabinet
User Manual
Germfree - Class II, Type A2 Biosafety Cabinet User Manual - v2.1 - 09SEP2021 - RMH

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  • Page 1 Class II, Type A2 Biosafety Cabinet User Manual Germfree - Class II, Type A2 Biosafety Cabinet User Manual - v2.1 - 09SEP2021 - RMH...
  • Page 2: Table Of Contents

    Germfree’s Mission ........
  • Page 3: Introduction

    Biomedical engineering and technology have introduced safeguards into the design of laboratory equipment, but these safeguards do not prevent human error. Danger to personnel from carelessness or improperly used equipment cannot be overemphasized. There is no substitute for safe, proper technique. Contact Germfree Customer Service +1 386.265.4300 cs@germfree.com...
  • Page 4: Installation

    Class II, Type A2 Biosafety Cabinet User Manual Installation The Class II, Type A2 Biosafety Cabinet is ordinarily transported and moved in the upright position. The 3’, 4’ and 6’ units will fit through any opening 32” wide and 58” high. If narrower doors are encountered, the light housing can be taken off by removing four screws.
  • Page 5: Electrical Services

    Class II, Type A2 Biosafety Cabinet User Manual Electrical Services The Class II, Type A2 Biosafety Cabinet operates on an 115v, 1-phase, 60 Hertz, 10 Amp electrical system. During shipment, the power cord is coiled within the control panel. The plug of the power cord is a NEMA 5-15P 3-pronged hospital grade and should be connected to the appropriate 3-prong 115v receptacle.
  • Page 6: Control Of Airflow

    The unit must also be recertified any time that it is relocated. The following chart represents the acceptable range of air velocities that are measured in your unit. Unit Inflow (fpm) Downflow (fpm) Volume (cfm) Exhaust Flow (fpm) BBF 2 87-102 65-80 114-134 154-181 BBF 3 102-112...
  • Page 7: Airflow Diagram

    Class II, Type A2 Biosafety Cabinet User Manual Airflow Diagram...
  • Page 8: Filters

    This requires sealing with RTV (Room Temperature Vulcanizing) silicone. Commercial organizations with proper equipment and personnel are available in most areas of the world for testing and servicing. If, you need us to refer you to one of these agencies please contact Germfree (cs@...
  • Page 9: Contoured Viewing Panel

    Class II, Type A2 Biosafety Cabinet User Manual Contoured Viewing Panel The shape of the viewing panel was designed to put the operator above the work with a glare free view of all parts of the work area. The viewing panels are hinged and can be raised to more than a 90-degree angle to provide easy access for larger items (shocks or chains are provided to support the window in the open position).
  • Page 10: Stainless Steel Work Deck

    Class II, Type A2 Biosafety Cabinet User Manual Stainless Steel Work Deck With the viewing panel removed (or raised and supported by the shocks / lifting chains), the work platform and its support can be easily separated and removed. If the Class II, Type A2 Biosafety Cabinet has been used with potential pathogens, the cabinet should first be decontaminated before disassembling.
  • Page 11: Motor Blower

    Class II, Type A2 Biosafety Cabinet User Manual Motor Blower The motors and blowers were selected because they have the following characteristics: low noise levels, low vibration levels, compatibility with the variable speed control, and most important: efficiency in delivering air through clean HEPA filters as well as those loaded with a considerable amount of particulate material.
  • Page 12: Lights

    Class II, Type A2 Biosafety Cabinet User Manual Lights Two fluorescent lights are provided in their own housing along the top of the viewing panel and exterior to the work area. This location gives the best glare free view to the operator and also prevents heat build up within the cabinet.
  • Page 13: Performance Testing

    Class II, Type A2 Biosafety Cabinet User Manual Performance Testing Your Class II, Type A2 Biosafety Cabinet features a one piece welded construction. No access panels are required and it has no jointed segments that might loosen during shipment. This is a distinct advantage because many potential sources of the leaks through gaskets are avoided.
  • Page 14: Use Of The Biosafety Cabinet

    Class II, Type A2 Biosafety Cabinet User Manual Use of the Biosafety Start-Up Procedure: Cabinet 1. Turn on unit 2. Wipe down all interior surfaces The successful use of the Class II, Type A2 Biosafety 3. Allow 10 minutes for particles to wash into HEPA Cabinet as a safety tool depends upon two factors: 4.
  • Page 15: Biological Safety Cabinet Operation "Don'ts

    Class II, Type A2 Biosafety Cabinet User Manual with open cuffs allow the entrapment of contaminated air around the technician’s arms inside the sleeves. The hands and arms should be inserted into and withdrawn from the work area slowly to prevent “dragging” of contaminants from the room into the work area or from the work area into the room.
  • Page 16: Troubleshooting

    Class II, Type A2 Biosafety Cabinet User Manual Troubleshooting This section is to be used as a general guideline. An independent certification agency or a qualified technician specializing in repairs and testing of this type of equipment must do any maintenance or repairs that need to be made.
  • Page 17 Class II, Type A2 Biosafety Cabinet User Manual Trouble Possible Causes Remedy Measure voltage – contact Low voltage on house current certification company Replace filters – contact Filter loaded certification company Adjust to proper reading on Low velocity air Improper adjustment of blower Magnehelic gauge –...
  • Page 18: Dimensions

    Class II, Type A2 Biosafety Cabinet User Manual Dimensions Overall Dimensions Width Depth Height BBF-2SSCH 24 ⅛” 23 ½” 46 ⅜” BBF-2SSRX 24 ⅛” 23 ½” 54 ⅛” BBF-3SSRX 36 ⅛” 27” 57” BBF-4SSRX 48 ⅛” 27” 57” BBF-6SSRX 72 ⅛” 27”...
  • Page 19: Parts List

    Parts List The following list contains reorder information for parts included on the base model. For information on optional features, or electrical components of the 220V model, please contact Germfree customer service at germfree.com Germfree part numbers are in red...
  • Page 20 Class II, Type A2 Biosafety Cabinet User Manual Motor 1/3 hp, 1625 rpm, 5.7 amps BBF-2SSCH / BBF-2SSRX / BBF-3SSRX / BBF-4SSRX Fasco # 7124-1126-1/2 182-0010-00 BBF-6SSRX 183-0043-00 Diffusors BBF-2SSCH / BBF-2SSRX 009-1100-02 BBF-3SSRX 009-1100-03 BBF-4SSRX 009-1100-04 BBF-6SSRX 009-1100-06 IV Bars Bar - All Models 003-6001-00 Bracket - All Models...
  • Page 21 Class II, Type A2 Biosafety Cabinet User Manual Capacitor 7.5 μF, 370 VAC UL Approved BBF-2SSCH / BBF-2SSRX / BBF-3SSRX / BBF-4SSRX AERVOX PART # P50G3707YJ6 152-0007-00 BBF-6SSRX Contact Customer Service Switches 10A-250 vac. 12A-125 vac. UL Approved All models CARLING SWITCH PART # T1GA21-6S-BL-NBL 170-0030-00 Motor Speed Control...
  • Page 22 Class II, Type A2 Biosafety Cabinet User Manual Light Housing BBF-2SSCH / BBF-2SSRX L-BBF-2SSRX BBF-3SSRX L-BBF-3SSRX BBF-4SSRX L-BBF-4SSRX BBF-6SSRX L-BBF-6SSRX Fluorescent Ballast Advance Transformer Ballast All models #1CN-2P32-SC35M 147-5007-00 Fluorescent Tubes BBF-2SSCH / BBF-2SSRX F-17T8/841 BBF-3SSRX F-25T8/741 BBF-4SSRX 32T8/741 BBF-6SSRX 32T8/741 Fluorescent Lamp Holders 660W 600V...
  • Page 23: Quality Statement

    User Manual Quality Statement Our Company Accountability Germfree has been a leading innovator in aseptic control and isolation systems since 1962. We will deliver our products on time, as promised, and free from defects. We design and manufacture a diverse range...
  • Page 24 Class II, Type A2 Biosafety Cabinet User Manual Appendix A Line Drawings...
  • Page 25 THIS PRINT IS PROVIDED ON A DIMENSIONS ARE IN MATERIAL FINISH RESTRICTED BASIS AND IS NOT TO INCHES. BE IS ANY WAY DETRIMENTAL TO DO NOT SCALE DRAWING. THE INTERESTS OF GERMFREE SHEET NAME SHEET MATERIAL DETAILS 1 OF 1 LABORATORIES THICKNESS...
  • Page 26 DIMENSIONS ARE IN MATERIAL FINISH RESTRICTED BASIS AND IS NOT TO INCHES. BE IS ANY WAY DETRIMENTAL TO DO NOT SCALE DRAWING. THE INTERESTS OF GERMFREE SHEET NAME SHEET MATERIAL DETAILS 1 OF 1 LABORATORIES THICKNESS \\file.germfree.local\projects\Engineering Vault\Employee Folders\Jafa, V\BBF Manual Drawings\BBF-2CH\E-GERM-003-2200-00.dft...
  • Page 27 THIS PRINT IS PROVIDED ON A DIMENSIONS ARE IN MATERIAL FINISH RESTRICTED BASIS AND IS NOT TO INCHES. BE IS ANY WAY DETRIMENTAL TO DO NOT SCALE DRAWING. THE INTERESTS OF GERMFREE SHEET NAME SHEET MATERIAL DETAILS 1 OF 1 LABORATORIES THICKNESS...
  • Page 28 THIS PRINT IS PROVIDED ON A DIMENSIONS ARE IN MATERIAL FINISH RESTRICTED BASIS AND IS NOT TO INCHES. BE IS ANY WAY DETRIMENTAL TO DO NOT SCALE DRAWING. THE INTERESTS OF GERMFREE SHEET NAME SHEET MATERIAL DETAILS 1 OF 1 LABORATORIES THICKNESS...
  • Page 29 THIS PRINT IS PROVIDED ON A DIMENSIONS ARE IN MATERIAL FINISH RESTRICTED BASIS AND IS NOT TO INCHES. BE IS ANY WAY DETRIMENTAL TO DO NOT SCALE DRAWING. THE INTERESTS OF GERMFREE SHEET NAME SHEET MATERIAL DETAILS 1 OF 1 LABORATORIES THICKNESS...
  • Page 30 Class II, Type A2 Biosafety Cabinet User Manual Appendix B Wiring Diagrams...
  • Page 33 Class II, Type A2 Biosafety Cabinet User Manual Appendix C Sensocon User Manual...
  • Page 34 Bulletin 102-0 Installation and Operation Manual Series A3 and A4 Differential Pressure Controller Sensocon, Inc. Phone: (863) 248-2800 Fax: (863) 248-2798 www.sensocon.com...
  • Page 35 Installation and Operation Series A3 & A4 Manual Contents 1. Introduction 1.1. Model Number Configuration 1.2. Specifications 1.3. Dimensional Drawings 2. Installation 2.1. Mounting 2.2. Pressure Connections 2.3. Electrical Connections 3. Operation 3.1. Display 3.2. Key Function 4. Programming 4.1. Menu Structure 4.2.
  • Page 36: Introduction

    Installation and Operation Series A3 & A4 Manual 1. INTRODUCTION The Sensocon A3 and A4 series of differential pressure controllers provide control of low differential pressure applications for air and other compatible non combustible gasses. These instruments have a large LED display for pressure indication, a LCD or OLED display for programming and additional LED’s to indicate % of output, setpoints and alarm status.
  • Page 37: Dimensional Drawings

    Installation and Operation Series A3 & A4 Manual Loop Resistance: 750 Ω Max (for internally sourced power); 1800 Ω Max (for externally sourced power of 36 VDC) Power Supply: Universal 16-265 VAC or VDC Housing Material: Glass Filled Nylon Enclosure Rating: Designed to meet NEMA 4X face; with optional cover the entire product is weatherproof Relays: (2) SPDT 8 Amps @ 250 VAC, 5A @30 VDC Electrical Connections: screw terminals...
  • Page 38: Installation

    Installation and Operation Series A3 & A4 Manual Optional weatherproof cover 2. INSTALLATION 2.1 Mounting Flush Mounting – For new applications, cut a 4 ” hole in the panel. Insert the control with the provided gasket through the hole and secure it to the panel with the provided mounting tabs and screws.
  • Page 39 Installation and Operation Series A3 & A4 Manual Surface Mounting – Surface mounting the Series A3 or A4 requires the optional weather proof cover. Once the control is wired and the weather proof cover has been attached, the control can be mounted to any flat surface with the four mounting screws provided with the cover.
  • Page 40: Pressure Connections

    Installation and Operation Series A3 & A4 Manual 2.2 Pressure Connections Two 3/16” pressure connections are located on the back of the unit, labeled “High” and “Low”. For best results, connect 3/16” I.D. push on tubing to the pressure connections. If the High connection has a greater absolute value than the Low connection, the front display will give a positive value.
  • Page 41: Operation

    Installation and Operation Series A3 & A4 Manual 3. OPERATION 3.1 Display The series A3 and A4 were designed to give the user maximum feedback and flexibility. The process arch LED’s are designed to mimic the indicating needle of a mechanical gauge. The process arch will light up from left to right as the pressure moves away from zero.
  • Page 42: Programming

    Installation and Operation Series A3 & A4 Manual To move from one program menu to another, hold the up arrow or down arrow for one second to move up or down one menu level. The control will start in the “Main”...
  • Page 43 Installation and Operation Series A3 & A4 Manual  Actuation 1  dir / rev 1 act  Set Point 1 Delay  value SP1 D  Set Point 2 Setting  db / lohi Set 2  Actuation 2 ...
  • Page 44 Installation and Operation Series A3 & A4 Manual variable. Below are the possible parameters that would be shown with the factory default program.  Set Point 1 Low (SP1 lo)  Set Point 1 High (SP1 hi)  Set Point 1 (SP1) ...
  • Page 45 Installation and Operation Series A3 & A4 Manual HiLo Operation Pressure Set Point Hi Set Point Lo Time Relay Action Direct Acting Reverse Acting Setpoint DB Operation Pressure DB direct acting DB reverse acting Set Point Time Relay Action Direct Acting Reverse Acting ...
  • Page 46 Installation and Operation Series A3 & A4 Manual  Set Point Delay (SP1 D & SP2 D) – This variable sets the minimum amount of time that the process must be above or below the set point for the switch state to actuate.
  • Page 47 Installation and Operation Series A3 & A4 Manual require a manual reset by holding the left arrow and right arrow simultaneously on the face of the control.  Automatic (Auto) – For automatic reset  Manual (Manual) – For manual reset ...
  • Page 48 Installation and Operation Series A3 & A4 Manual  (kPa) – Kilopascals  (PSI) – Pounds per square inch  (inHg) – Inches of mercury  (mmHg) – Millimeters of mercury  (mBAR) – Millibars  (ftWc) – Feet of water column ...
  • Page 49 Installation and Operation Series A3 & A4 Manual  Dampening (Damp) – The control takes a pressure sample every 100 milliseconds. The dampening coefficient tells the control how many readings to average for the displayed value. Displaying readings with too few samples averaged may cause unstable readings as a result of vibration or pressure fluctuations.
  • Page 50 Installation and Operation Series A3 & A4 Manual 4.4 Secure Menu  Zero (Zero) – This is a calibration parameter that allows re-calibration of zero. To re-zero the control, disconnect both pressure connections so they are open to atmospheric pressure and reset the value by holding the accept key.
  • Page 51: Other Features

    Installation and Operation Series A3 & A4 Manual  Process Output Low (pol) – This parameter allows the 4-20 mA output to be scaled. The value set for this parameter will correspond to the 4 mA output. The default setting will be 0, but can be set at any value lower than Output High.
  • Page 52: Limited Warranty

    Installation and Operation Series A3 & A4 Manual PK-02 lockout programming key, a user may completely lock (or unlock) the face keypad of the control to eliminate the possibility of unwanted tampering of the control. To download programs from a control to a programming key, place the key within 1 to 6 inches of the OptiLink™...

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