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Summary of Contents for Germfree BBF Series
- Page 1 Benchtop Bioflow Chamber (BBF) User Manual (Class II, Type A/A2)
- Page 2 Germfree Laboratories 4 Sunshine Blvd • Ormond Beach, FL 32174 Phone 386.265.4300 • Fax 386.677.0442 www.germfree.com...
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Page 3: Table Of Contents
A P P E N D I X B Filters L I N E D R A W I N G S BBF 2 BBF 3 BBF 4 BBF 6 Wiring Diagram P A R T S L I S T Standard BBF Series 220v... -
Page 5: Introduction
Introduction The field of microbiology has recently moved into its second century. Many of the principles originated by Pasteur, Koch, Lister and others are still valid and in use today. In the past century the investigator, clinician, and/or technologist performed work without assessing the hazards to the experimenter, to the experiment or to the public. - Page 6 gloves. This is accomplished by discharging approximately 30% of the total filtered air each cycle through the exhaust HEPA filter in the top of the unit. An equal amount of make-up air is pulled into the chamber through the bottom of the window under negative pressure from the blower and positive pressure from the air curtain.
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Page 7: Installation
Installation The BENCHTOP BIOFLOW CHAMBER is ordinarily transported and moved in the upright position. The exhaust filter of the BENCHTOP BIOFLOW CHAMBER is located within the unit and the dimensions are such that it will fit through any opening 32” wide and 58” high. If narrower doors are encountered, the light housing can be taken off by removing four screws or the viewing panel with the attached light housing can be completely removed by sliding the split hinges to the right. -
Page 8: Control Of Airflow
Any time a concern about the cabinet airflow is present you should call the GERMFREE’s customer service department or your local certifier for advice prior to resuming operation in the unit. The... - Page 9 performed at least annually, and many regulatory bodies recommend semi-annually. The following chart represents the acceptable range of air velocities that are measured in your unit. UNIT INFLOW DOWNFLOW VOLUME EXHAUST FLOW (fpm) (fpm) (cfm) (fpm) BBF 2 87-102 65-80 106-125 174-204 BBF 3...
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Page 10: Airflow Diagram
Air Flow Diagram... -
Page 11: Filters
Filters All BIOFLOW CHAMBERS are provided with both a supply HEPA filter over the work area and an exhaust HEPA filter in the top of the unit. All filters were subjected to a particle challenge leak test at the time of manufacturing and again immediately before shipment and are certified to be 99.99% effective on a .3 Micron particle, the hardest to capture. -
Page 12: Work Platform
to the floor and slide the panel toward the right side, completely separating the split hinges. Be sure to replace snap ring after use. Work Platform With the viewing panel removed (or raised and supported by the lifting chains), the work platform and its support can be easily and separated and removed. - Page 13 prevents heat build-up within the cabinet. The lamps are wired independent of the motor-blower and provide good visibility within the work area at all times. Ultraviolet lights are provided in the work area only if ordered. Most authorities do not recommend use of UV light in this type of safety cabinet for a number of reasons: 1) A considerable volume of HEPA filtered air passes through the work area so that the unit tends to be self-cleansing.
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Page 14: Performance Testing
predetermined interval, e.g., 10 to 20 minutes. Preferably, the user should set an alarm type clock and stay out of the immediate vicinity of the chamber when the UV lights are operative. Standard precautions must be taken, of course, to protect personnel whenever ultraviolet lights are utilized. Performance Testing Your BENCHTOP BIOFLOW CHAMBER features a one-piece welded construction. - Page 15 senior investigator or the head of a particular facility to train the personnel who will use the unit and to see that good technique is maintained. If this is not done, a false sense of security may prevail. Advance Planning: To achieve maximum safety and utilization of your BENCHTOP BIOFLOW CHAMBER you must take into account the equipment and materials necessary for the proposed operation and must outline the procedural details of your particular operation or experiment.
- Page 16 should be washed thoroughly with a germicidal soap. It is also recommended that technicians working in the BENCHTOP BIOFLOW CHAMBER wear long-sleeved gowns with knit cuffs and rubber gloves. This minimizes the shedding of skin flora into the work area and protects the hands and arms from contamination with viable agents.
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Page 17: Troubleshooting
into turbulent patterns). Also do not reduce the airflow rate below safe levels. 3) DON’T ever cover the exhaust filter. 4) DON’T ever attempt any maintenance of the motor or any component outside the work area without decontaminating the unit as described in Appendix B. - Page 18 Break in electrical wiring Use electrical diagram and check wiring connections. Excessive blower vibration Foreign matter in blower Remove paper, paper clip, etc. squirrel cage Exhaust air imbalance Obstruction over exhaust filter Remove obstruction. Improper adjustment of Adjust to proper reading on blower motor control Magnehelic gauge.
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Page 19: Paraformaldehyde Decontamination20
Appendix A P A R A F O R M A L D E H Y D E D E C O N T A M I N A T I O N Successful decontamination with paraformaldehyde consists of exposing all internal parts of the chamber to the generated formaldehyde gas under the proper conditions, which will kill all microorganisms that are harbored anywhere within the cabinet. - Page 20 however, that decontamination with paraformaldehyde will occur at a relative humidity as low as 50% and a temperature as high as 30C. If the relative humidity is too low it can be increased by boiling water on a hot plate within the work area. A commercially available electric frying pan, containing a specified amount of paraformaldehyde (see table) is placed on the work tray.
- Page 21 micro-organisms on the surfaces and in the filter. However, during this study, the exhaust blower was turned on four times because the previous experience of others as well as ourselves, proved the importance of disseminating the formaldehyde gas during this decontamination.
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Page 22: Filters
Appendix B F I L T E R S An independent certification agency or a qualified technician specializing in the repair and testing of this type of equipment must perform all maintenance to the BENCHTOP BIOFLOW CHAMBER, especially the filters. I. - Page 23 Fill grooves of multigrooved bottom gasket with silicone grease (can or jar supplied). Lower into cabinet making sure that the arrow and the silicone filled gasket is facing down. Reassemble using reverse procedures to Section A above. C. Installation Exhaust Filter Examine for possible damage.
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Page 24: Line Drawings
Line Drawings B B F 2... - Page 25 B B F 3...
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Page 26: B B F 4
B B F 4 B B F 6... -
Page 28: Wiring Diagram
Wiring Diagrams B B F... -
Page 29: Parts List
Parts List HEPA Filters Supply Exhaust 2-RX 20" x 20" x 3" 18" x 8" x 6" 3, BBF 3-RX 20" x 34" x 6" 18" x 12" x 6" 4, BBF 4-RX 20" x 46" x 6" 18" x 16" x 6" 6, BBF 6-RX 20"... - Page 30 Circuit Breaker UL & CSA Approved POTTER & BROOMFIELD PART #W58-XB1A4A-XX XX= RATED AMPS Duplex Electrical Outlet 15A-15V UL APPROVED LEVINGTON PART # 5320-I Fluorescent Ballast Advance Transformer Ballast #1CN-2P32-SC35M Fluorescent Tubes 2 Ft: F-17T8/841 3Ft: F-25T8/741 4,6 & 8 Ft: 32T8/741 Fluorescent Lamp Holders 660W 600V UL Approved...
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Page 31: Bbf Series 220V
B B F S E R I E S 2 2 0 V HEPA FILTERS: SUPPLY EXHAUST BBF 2SS 20” x 20” x 3” 8” x 18” x 6” BBF 3 SS 20” x 34” x 6” 12” x 18” x 6” BBF 4SS 20”... - Page 32 Dwyer Instruments # 2002 LIGHTING: Fluorescent Bulbs GE # FF20T12-CW, GE # F40CW-RS-WM or Phillips # F30TP/CW/RS Ultraviolet Bulbs Bulb Direct # G15T8 Code 11078 Ballast-Fluorescent Magnetek # 673-L, 544-L-TC or 437-L-TC-P Ballast-Ultraviolet, 220 volt, rapid start Advance Transformer # XM-2SP20-TP Lamp Holder Leviton # 13357-N Jones Plug (UL approved)
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