Telstar Clean Air EuroFlow Series User Manual
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Telstar Clean Air EuroFlow Series User Manual

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User manual
English
Version 10.0
(valid for software V4.9 and higher)
Series EuroFlow,
Type EF
Biological Safety Cabinet Class II
&
Type EF/B
Biological Safety Cabinet Class II
Types:
EF
EF 4
EF 5
EF 6
EF/B 4
EF/B 6
Offers protection for personnel,
environment and product.
Before using the safety cabinet, please read
this manual carefully. The required
instructions have to be carried out first.
EF/B
Clean Air Techniek B.V.
Kuipersweg 37
3449 JA Woerden
Nederland
t: +31 (0) 348 41 11 14
f: +31 (0) 348 42 26 84
e: info@cleanair.eu
i: www.cleanair.eu

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Summary of Contents for Telstar Clean Air EuroFlow Series

  • Page 1 User manual English Version 10.0 (valid for software V4.9 and higher) Series EuroFlow, Type EF Biological Safety Cabinet Class II & Type EF/B Biological Safety Cabinet Class II Types: EF 4 EF 5 EF 6 EF/B 4 EF/B 6 Offers protection for personnel, environment and product.
  • Page 2 EuroFlow EF and EF/B Biological Safety Cabinet Preface Congratulations with your new biological safety cabinet Class II, Series EuroFlow, type EF and/or type EF/B. This Dutch quality product is designed, produced and tested by Clean Air Techniek B.V., in our production facility in Woerden, The Netherlands. The primary function of the safety cabinet is safety for operator, product and environment.

  • Page 3: Table Of Contents

    EuroFlow EF and EF/B Biological Safety Cabinet Introduction_______________________________________________ 6 EF ___________________________________________________________________6 EF/B _________________________________________________________________7 Safety____________________________________________________ 8 Use in conformity with regulations _________________________________________8 Modifications and changes to the installation _________________________________8 Authorized persons _____________________________________________________8 General safety aspects __________________________________________________9 Cytotoxic danger (EF/B only) ____________________________________________10 Explanation user safety symbols __________________________________________10 Installation ______________________________________________ 11 Assembly-instruction ___________________________________________________11...
  • Page 4 EuroFlow EF and EF/B Biological Safety Cabinet Control __________________________________________________ 22 Using the cabinet______________________________________________________22 Top hood/sliding window _______________________________________________22 Display interface ______________________________________________________25 Starting up the cabinet _________________________________________________26 Locking functions cabinet _______________________________________________27 Power save mode _____________________________________________________28 Turn off _____________________________________________________________28 Control UV (optional)___________________________________________________29 Control gas supply (optional) ____________________________________________30 5.10 Control sockets _______________________________________________________31 5.11 Menu functions _______________________________________________________31 5.12 General Information ___________________________________________________32...
  • Page 5 EuroFlow EF and EF/B Biological Safety Cabinet Requirements ______________________________________________________________74 Appendix V: Measuring method air velocities ________________________ 76 Air velocities downflow_______________________________________________________76 Air velocities inflow* ________________________________________________________76 Appendix VI: Lay-out boards _____________________________________ 78 Appendix VII: Alarm-test procedure _______________________________ 79 Appendix VIII: Stickers _________________________________________ 81 Appendix IX: Information installed options__________________________ 84 Exhaust hood ______________________________________________________________84 Installed options microprocessor _______________________________________________84...

  • Page 6: Introduction

    EuroFlow EF and EF/B Biological Safety Cabinet Introduction The EF is a class II Biological Safety Cabinet. It gives the user the possibility to work safely and sterile with bioagentia without complete physical barrier between person and experiment (through work opening (C)). •...

  • Page 7: Ef/B

    EuroFlow EF and EF/B Biological Safety Cabinet EF/B The EF/B is a class II Biological Safety Cabinet. It gives the user the possibility to work safely and sterile with bioagentia without complete physical barrier between person and experiment (through work opening (C)). •...

  • Page 8: Safety

    EuroFlow EF and EF/B Biological Safety Cabinet Safety 2.1 Use in conformity with regulations Personnel that is not properly informed about the safety regulation and/or does not meet the §2.3 Authorized persons requirements mentioned in , is not allowed to use the cabinet. Improper use of the cabinet is not allowed.

  • Page 9: General Safety Aspects

    EuroFlow EF and EF/B Biological Safety Cabinet • They are sufficiently informed about the operation of the installation and the operation of the sub-systems. 2.4 General safety aspects 1. The installation shall never be used for any (micro-) biological or any other potentially dangerous work, unless the fan is switched into working mode, the sliding window is in working position and there is no alarm.

  • Page 10: Cytotoxic Danger (Ef/B Only)

    EuroFlow EF and EF/B Biological Safety Cabinet 2.5 Cytotoxic danger (EF/B only) Before starting work in cytotoxics production the hazard risks of cytotoxics handling need to be evaluated and documented (industrial safety act, hazardous substances regulations). Based on these findings the employees must be instructed. 2.6 Explanation user safety symbols GENERAL DANGER You can harm yourself and others seriously if you...

  • Page 11: Installation

    EuroFlow EF and EF/B Biological Safety Cabinet Installation GENERAL DANGER Be careful: high centre of gravity, crossover point. Never hinge the window, as long as the cabinet is not placed on the support frame The cabinet must be installed in a safe way. Clean Air Techniek B.V. supplies an optional support frame, which is designed for this purpose.

  • Page 12: Transport Instructions

    EuroFlow EF and EF/B Biological Safety Cabinet In case of maintenance the cabinet must be completely free of voltage. This means that the connector in the socket must be easy to reach, otherwise a main-switch must be mounted between the cabinet and the power supply so that the cabinet can be cut off of the power-circuit. 3.2 Transport instructions Before moving the cabinet make sure the power cable is disconnected of the power supply The cabinet must be transported on a strong solid pallet.

  • Page 13: Product Description

    EuroFlow EF and EF/B Biological Safety Cabinet Product description Figure 3 Sectional view EF Figure 4 3D view EF Figure 5 Sectional view EF/B Figure 6 3D view EF/B UM-EFAB-EN10.doc...

  • Page 14: Type Plate

    EuroFlow EF and EF/B Biological Safety Cabinet Part list EF and EF/B Type plate Short user manual Body of the cabinet Hinge/slide window Top hood Worktop Aerofoil Plenum-construction Drip tray/pre-filter 10 Downflow filter 11 Downflow protection (build in) 12 Exhaust filter 13 Support frame (optional) 14 Feet support 15 Front panel...

  • Page 15: Hinge-Slide Window

    EuroFlow EF and EF/B Biological Safety Cabinet 4.4 Hinge-slide window Figure 3, Figure 4, Figure 5 Figure 6, The hinge-slide window ( Pos. 4) is a tightly closing, manually operated glass window. It can be slid up or down, and opened and closed by means of handgrips.

  • Page 16: Drip Tray/Pre-Filter (Only Ef/B And As An Option For The Ef)

    EuroFlow EF and EF/B Biological Safety Cabinet 4.9 Drip tray/pre-filter (only EF/B and as an option for the EF) Figure 3 Figure 5, The pre-filter with drip tray (EF: and EF/B: Pos. 9) is assembled under the worktop. The installation is equipped with this to protect the interior of the installation against pollution by coarse materials and to collect spilled fluids.

  • Page 17: Back Panel

    EuroFlow EF and EF/B Biological Safety Cabinet • the fan. GENERAL DANGER / BIOLOGICAL DANGER Cabinet must be disinfected before removing the front panel 4.16 Back panel Figure 3, Figure 4, Figure 5 Figure 6, The back panel ( Pos. 16) can be dismantled to get access to: •...

  • Page 18: Operating Panel

    EuroFlow EF and EF/B Biological Safety Cabinet 4.19 Operating panel Figure 7 Operating panel Figure 3, Figure 4, Figure 5 Figure 6, Figure 7 The operating panel ( Pos. 19 and ) consists of: 1. Display; 2. Function keys (4x); 3.
  • Page 19 EuroFlow EF and EF/B Biological Safety Cabinet installation makes sure that the valve can only be operated in work mode and will be switched off automatically by leaving this mode. The gas valve switch-function is only visible when the fan in work mode is and there are no air speeds alarms.
  • Page 20 EuroFlow EF and EF/B Biological Safety Cabinet • The connection, that is located outside the installation, is standard assembled in the bottom of the installation. • The connection, that is located outside the installation, can be assembled by customer request in left or right side panel or top of the installation.

  • Page 21: Sockets

    EuroFlow EF and EF/B Biological Safety Cabinet When the UV is switched on, the UV hour counter counts the lighting period. UV-lamps have, depending on fabricate and type, a limit in effective running hours. Replace the lamp frequently. Alternative Options Please contact Clean Air Techniek B.V.

  • Page 22: Control

    EuroFlow EF and EF/B Biological Safety Cabinet Control 5.1 Using the cabinet Check if the information on the type plate is corresponding to the main power connection. Connect the cabinet to the main connection by putting the plug into a grounded socket that is easy accessible.
  • Page 23 EuroFlow EF and EF/B Biological Safety Cabinet Top position: Maximum opening, to place objects in cabinet. Figure 8 Window positions Slide the window To slide the window, both handles need to be used. Note 1: When the window is not in the working position, the safety of the cabinet cannot be granted.
  • Page 24 EuroFlow EF and EF/B Biological Safety Cabinet Figure 10, • Hinge (tilt) up the window (see Pos. 6) with the aid of the springs, it stays open on the maximum position please use both hands for lifting the window! Figure 10 Hinge instructions Figure 11 Locking pins Figure 12 Hinge position Figure 13 Slide position...

  • Page 25: Display Interface

    EuroFlow EF and EF/B Biological Safety Cabinet 5.3 Display interface Figure 17 Display Interface Figure 17 The operating panel (Figure 4 and Figure 6 Pos. 19 and ) consists of: 1. Display; 2. Function keys (4x); 3. Fixed keys (4x); 4.

  • Page 26: Starting Up The Cabinet

    EuroFlow EF and EF/B Biological Safety Cabinet All equipment and, if possible, all materials required for working with a safety cabinet need to be placed into the installation before starting up the cabinet. Equipment or objects with a height up to 350 mm can be placed in the working space by sliding the window upwards.

  • Page 27: Locking Functions Cabinet

    EuroFlow EF and EF/B Biological Safety Cabinet If the air speeds still are not Starting to work mode – not safe right, the alarm will sound again. To see what is wrong: Monday 11:06:33 MENU Socket=Off • Push button (Menu). A =>...

  • Page 28: Power Save Mode

    EuroFlow EF and EF/B Biological Safety Cabinet code: acknowledgement the cabinet has been locked. Appendix X: User code on page 87. To unlock the cabinet, the user code has to be entered again. 5.6 Power save mode When not using the cabinet for a short period of time, put it in the power save mode. The fan will run at half speed to save energy (synonyms for power save mode are standby mode or night mode).

  • Page 29: Control Uv (Optional)

    EuroFlow EF and EF/B Biological Safety Cabinet The alarm indicator becomes red and there is an acoustic alarm. • Slide the sliding window on to the aerofoil. Cabinet in work mode – not safe Window is not in the working position MENU Socket=Off The display will ask for the user...

  • Page 30: Control Gas Supply (Optional)

    EuroFlow EF and EF/B Biological Safety Cabinet The UV will switch on. • Push button (UV=Off). The display shows “UV=On”. Cabinet switched off – Not Safe Microbiological Safety Cabinet UV switched on MENU Socket=Off UV=On Switching UV off The UV will switch off. •...

  • Page 31: Control Sockets

    EuroFlow EF and EF/B Biological Safety Cabinet Cabinet switched off – Not Safe Microbiological Safety Cabinet Gas switched off MENU Socket=Off Gas=Off 5.10 Control sockets The sockets can always be switched on and off. If the cabinet is switched off, the sockets are automatically switched off.

  • Page 32: General Information

    EuroFlow EF and EF/B Biological Safety Cabinet 5.12 General Information In general information more details are given about: • Hour counter information and reset (FL, UV, Timer) • Set up 7 days clock UV (Example) • Set up socket 7 days clock •...
  • Page 33 EuroFlow EF and EF/B Biological Safety Cabinet • Push button (Reset). • Push 2x button (Esc) for starting menu. Reset FL counter? • Push button (reset A). • Push button (Reset). • Push 2x button (Esc) for starting menu. Reset UV counter? •...
  • Page 34 EuroFlow EF and EF/B Biological Safety Cabinet 2c. Disable Timer The timer sets a period until the • Push button (Timer), to set up the timer. UV turns off. • Push button (Change), to change the time. • The value can be changed by pushing button (>>), (+) or (-).
  • Page 35 EuroFlow EF and EF/B Biological Safety Cabinet A => Hour counter information B => Status cabinet C => Settings • Push button (A). Hour counter information A = Enter => <= • Push button (=>). Set up UV 7 days clock Set up for 7 days clock UV Function: Clock shows.
  • Page 36 EuroFlow EF and EF/B Biological Safety Cabinet -Change stop min (00.00). Tuesday UV on: 22:00 UV off: 00:00 A = Enter >> • Acknowledge the setting with button Set up UV switching off on Hour counter information Wednesday morning 06.00. -Change stop day, A = Enter -Change start hour (00.00),...
  • Page 37 EuroFlow EF and EF/B Biological Safety Cabinet • • The value can be checked by pushing button (>>) change day. Every days program can be controlled by pushing (+) or (-). • Important! Don’t touch , only use 2x button (Esc) to go back to the main menu.
  • Page 38 EuroFlow EF and EF/B Biological Safety Cabinet There are three ways to set the sockets: Timer 7 days clock Disable §5.11 Set up 7 days clock The timer function needs to be activated first, otherwise the UV on page 33 and further. sockets will not switch on.
  • Page 39 EuroFlow EF and EF/B Biological Safety Cabinet Change hours Cabinet to work mode at 10.00 A = Enter >> • The value can be changed by pushing button (-). Change minutes: Thursday Cabinet to work mode at 10.00 => • Push button (=>) again, to change the minutes.

  • Page 40: Information Status Cabinet

    EuroFlow EF and EF/B Biological Safety Cabinet • The day can be changed by pushing button (+) or (-). Change hours/min/sec: • Push button (>>) again. • The hours/min/sec can be changed by pushing button Set up display set up the actual (+) or (-).
  • Page 41 EuroFlow EF and EF/B Biological Safety Cabinet Status information cabinet shows (see example). • Middle numbers are the actual air speeds (yellow); • Left and right numbers are the set up alarm borders (red). Information fan shows • Push button (=>).

  • Page 42: Settings

    EuroFlow EF and EF/B Biological Safety Cabinet Options Screen I Installed Options Starting up from Step 2 1 Socket present, UV inst. Gas present, 2 flowsens., Biohazard:Yes => <= Option Installed options display shows. • Push button (Option). Option Exhaust fan Relays #C2 Appendix IX: Information For explanation codes see...
  • Page 43 EuroFlow EF and EF/B Biological Safety Cabinet • Preparations for disinfection • Activating the user code • Factory adjustments • Testing of alarms • Change the user code • Change language Disinfection Your Biological Safety Cabinet has a “built-in” disinfection software program. This program knows a number of varieties, which are dependent on the installed options in the security cabinet, and the manner the cabinet has been installed.
  • Page 44 EuroFlow EF and EF/B Biological Safety Cabinet button • Push button (check OK) The aerofoil has to be removed out of the work area and the window has to be closed completely. The valve on the exhaust (if present) has to closed. •...
  • Page 45 EuroFlow EF and EF/B Biological Safety Cabinet Testing of alarms Appendix VII: Alarm-test procedure on page 79. For service purposes only! More information in Cabinet in work mode. Cabinet in work mode - Safe §5.4 Starting up the cabinet Monday 11:06:33 on page 26.
  • Page 46 EuroFlow EF and EF/B Biological Safety Cabinet Change language To change the language, first close the window, switch off the cabinet and all functions. Then the language can be changed and confirmed. As confirmation the 3 LED’s (right) light up for 1 second and 2 short beeps will sound.

  • Page 47: Alarms

    EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. Alarms Figure 18 Operating panel When there is an alarm situation the alarm indicator flashes red and there is an acoustic signal. The safe working conditions are no longer granted. Push button for giving an acknowledgement that the user has seen the alarm.

  • Page 48: Flow Alarms

    EuroFlow EF and EF/B Biological Safety Cabinet Window below window position: Person protection stays intact. Product protection cannot be granted. The inflow air through the work opening has a higher speed and may pass the front air slits of the working surface.

  • Page 49: Other Alarms

    EuroFlow EF and EF/B Biological Safety Cabinet Inflow too high Inflow too high Figure 23 Person protection stays intact. Product protection becomes less. The inflow air through the work opening has a higher speed and may pass the front air slits of the working surface.
  • Page 50 EuroFlow EF and EF/B Biological Safety Cabinet Procedure in case of alarms Check if the window is in the working position. When the window is back into the working position the alarm will not mute immediately. The cabinet needs time to get the correct airflows.

  • Page 51: Cleaning

    EuroFlow EF and EF/B Biological Safety Cabinet Cleaning CYTOTOXIC DANGER §2.5 Cytotoxic danger (EF/B only) on page When cytotoxics are used, see Only the responsible safety officer (biological safety officer or the responsible examiner) can decide if this procedure is valid or not. Additional standard operation procedures can be necessary.
  • Page 52 EuroFlow EF and EF/B Biological Safety Cabinet Put on gloves; §7.1 Cleaning the installation on page 51); Clean the top side of the aerofoil (see Take the aerofoil out the front slit and take the aerofoil outside the cabinet. Take care whether Appendix II: you can take out the aerofoil, does the cabinet have to be disinfected first? (See Disinfection on page 67...

  • Page 53: Cleaning The Uv-Light (Optional)

    EuroFlow EF and EF/B Biological Safety Cabinet Figure 27 Fold the worktop against the back partition Clean the sides and bottom part of the worktop; Fold the worktop back in place. Cleaning the inside of the window Check if this procedure can be done safely. It is advised to disinfect the installation first (see Appendix II: Disinfection on page 67 Procedure for cleaning and to disinfect the window with liquid: Put on gloves;...

  • Page 54: Guideline For Cleaning Of Cytotoxic Contaminated Surfaces (Ef/B Only)

    EuroFlow EF and EF/B Biological Safety Cabinet The UV-light must be cleaned regularly with alcohol (70%). Put on gloves and switch of the cabinet and let the UV-light cool down before cleaning. After cleaning the UV-light tube must be dried before using it. 7.3 Guideline for cleaning of cytotoxic contaminated surfaces (EF/B only) CYTOTOXIC DANGER §2.5 Cytotoxic danger (EF/B only) on page...
  • Page 55 EuroFlow EF and EF/B Biological Safety Cabinet Chloormethine 51-75-2 (Mechlorethamine) 0,1 N Natronloog (Sodium Dactinomycine 50-76-0 Hydroxide) CAS-no. 1310-73-2. Estramustine 2998-57-4 Example 3: Cytotoxic CAS-no. Recommended cleaner Mitomycine C= 50-07-7 0,1 N HCL (Hydrochloric Acid) CAS-no. 7647-01-0. UM-EFAB-EN10.doc...

  • Page 56: Small Maintenance And Testing

    EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. Small maintenance and testing BIOLOGICAL DANGER BE CAREFUL!! Before starting service with the cabinet a written declaration “that the cabinet is decontaminated” must be present. The responsible person (laboratory supervisor) will decide if the cabinet has to be decontaminated so that when servicing the installation and the HEPA-filters there is no danger for the service engineer, labour personnel, the laboratory and the surroundings of the cabinet.
  • Page 57 EuroFlow EF and EF/B Biological Safety Cabinet Supplies needed Usermanual; Plastic bag (minimum measurement 75x50 [cm] with closure); Two pairs of gloves; Tape; Working surface hook (auxiliary hook); Possible disposing requirements; New pre-filter (measurements of the pre-filter are necessary when ordering a new one, the §4.1 Type plate on page 14 Appendix measurements are printed on the type plate (see...
  • Page 58 EuroFlow EF and EF/B Biological Safety Cabinet Figure 30 Lifting up the worktop against the back partition Place the opening of the plastic bag inside the front aperture with your left hand; Figure 31 Positioning the left hand in the plastic bag Figure 32 Positioning the plastic bag on the aerofoil Positioning the plastic bag on the aerofoil;...

  • Page 59: Replacing The Fluorescent-Light (Fl Light)

    EuroFlow EF and EF/B Biological Safety Cabinet Figure 33 Put the filter into the bag Fold back the edge of the plastic bag, not touching the inner side of the plastic bag; Press the air out of the bag (with its opening still in the working space); Seal the plastic bag airtight;...

  • Page 60: Service Contract

    EuroFlow EF and EF/B Biological Safety Cabinet page 14 page 81 (for the position of the type plate see • Version number of the software used in the safety cabinet. Software Version Cabinet in work mode. Cabinet in work mode - Safe §5.4 Starting up the cabinet Monday 11:06:33 on page 26.

  • Page 61: Technical Specifications

    EuroFlow EF and EF/B Biological Safety Cabinet Technical specifications 9.1 Physical surrounding EF 4 EF 5 EF 6 EF/B 4 EF/B 6 Transport physics [°C] From -25 up to From -25 up to From -25 up to From -25 up to Surrounding temperature From -25 up to 55 Surrounding temperature...
  • Page 62 EuroFlow EF and EF/B Biological Safety Cabinet EF 4 EF 5 EF 6 EF/B 4 EF/B 6 Filter Pre-filter [mm] G3 (EN 779) G3 (EN 779) G3 (EN 779) G3 (EN 779) G3 (EN 779) 1150x445 1460x445 1760x445 1150x445 1760x445 Downflow filter [mm] Astrocell...
  • Page 63 EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. EF 4 EF 5 EF 6 EF/B 4 EF/B 6 Electrics Power connection 230 +/- 5% 230 +/- 5% 230 +/- 5% 230 +/- 5% 230 +/- 5% [Hz] Needed main fuse box security J6: Alarm, potential free...
  • Page 64 EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. EF 4 EF 5 EF 6 EF/B 4 EF/B 6 Lighting TL-High output Colour White – Nr.840 White – Nr.840 White – Nr.840 White – Nr.840 White – Nr.840 Diameter [mm] Ø26...

  • Page 65: Trouble Shooting

    EuroFlow EF and EF/B Biological Safety Cabinet Trouble shooting Fault Cause Remedy No power on socket of the cabinet Socket is not activated Activate socket on display push on Fuse is broken Replace the fuse UV button is not visible on display UV equipment is not installed Option UV is not installed UV option has to be programmed please...

  • Page 66: Appendix I: Support Frames Ef & Ef/B

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix I: Support frames EF & EF/B For every type of cabinet from the EF-series and the EF/B series a specific support frame is available. Figure 35 Example of a support frame (EF 6) UM-EFAB-EN10.doc...

  • Page 67: Appendix Ii: Disinfection

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix II: Disinfection Your Biological Safety Cabinet has a “built-in” disinfection software program. This program knows a number of varieties, which are dependent on the installed options in the security cabinet, and the manner the cabinet has been installed. The disinfection software of the control print is only available in version number 2.4 270307 or higher.

  • Page 68: Appendix Iii: Replacement Of The Hepa Filters

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix III: Replacement of the HEPA filters Under normal circumstances, the life span of a HEPA filter comprises several years. However, it will become clogged up gradually, until there is no longer a proper flow-through and the filter has to be replaced.

  • Page 69: Replacement Of Hepa Filters

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix V: the measured values should deviate more than 20% from the average value. See Measuring method air velocities on page 76 for more specifications. • In case HEPA filter Integrity is measured with a photometer and DOP, EMERY 3004 or DEHS as test aerosol, then the maximum local penetration should not exceed 0,01%.
  • Page 70 EuroFlow EF and EF/B Biological Safety Cabinet Take note of this warning and advice since these activities are potentially harmful!! Replacement of the downflow HEPA filter For replacement of the downflow HEPA filter the following procedure must be followed: Supplies needed: •...
  • Page 71 EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. Position the new downflow HEPA filter • Unpack the new filter, check specifications and register the serial number and specifications; • Filter certificate has to be stored on a safe place; •...
  • Page 72 EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. Position the new exhaust HEPA filter • Unpack the new filter, check specifications and register the serial number and specifications; • Filter certificate has to be stored on a safe place; •...
  • Page 73 EuroFlow EF and EF/B Biological Safety Cabinet Removing the old 1 HEPA filter sections of a pressure frame • Remove the pressure frame of a filter section; • Seal both the openings of the two HEPA filters with DUCT-tape or similar, in order to isolate the contaminated side of these two HEPA filters from the environment;...

  • Page 74: Appendix Iv: Efficiency Test Of The Hepa Filters

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix IV: Efficiency test of the HEPA filters BIOLOGICAL DANGER BE CAREFUL!! Before starting service with the cabinet a written declaration “that the cabinet is decontaminated” must be present. The responsible person (laboratory supervisor) will decide if the cabinet has to be decontaminated so that when servicing the installation and the HEPA-filters there is no danger for the service engineer, labour personnel, the laboratory and the surroundings of the cabinet.
  • Page 75 EuroFlow EF and EF/B Biological Safety Cabinet Test aerosol for the downflow and exhaust filters can be added on the special connection on the Figure 39) front of the first HEPA filter section ( . The 100% measure point upstream concentration Figure 37, for the downflow filter as well as the exhaust filter is positioned under the work area ( Figure 38...

  • Page 76: Appendix V: Measuring Method Air Velocities

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix V: Measuring method air velocities BIOLOGICAL DANGER BE CAREFUL!! Before starting service with the cabinet a written declaration “that the cabinet is decontaminated” must be present. The responsible person (laboratory supervisor) will decide if the cabinet has to be decontaminated so that when servicing the installation and the HEPA-filters there is no danger for the service engineer, labour personnel, the laboratory and the surroundings of the cabinet.
  • Page 77 EuroFlow EF and EF/B Biological Safety Cabinet Figure 40 Side view for measuring level inflow *Measuring probe Figure 41 Measuring points inflow (H = Height, B = Broad) The average inflow velocity at the top of the front aperture is measured and can be read in the display. The minimal required value of the average inflow velocity measured at the top of the front aperture is given in chapter 9.2 on page 61.

  • Page 78: Appendix Vi: Lay-Out Boards

    EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. Appendix VI: Lay-out boards Interface board Figure 44 Interface board Control board Figure 45 Control board E010001 rev F UM-EFAB-EN10.doc...

  • Page 79: Appendix Vii: Alarm-Test Procedure

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix VII: Alarm-test procedure (Authorized personnel only) To test the alarm limits, the automatic fan control must be overruled, so the fan power can be set by hand. The entire state of the cabinet will stay unchanged, except for the fan-power. The required test-code is 7733.
  • Page 80 EuroFlow EF and EF/B Biological Safety Cabinet Middle numbers are the actual values from the airspeed indicators, which are changeable with the use of the buttons (+) and (-). First and last values are the set up alarm borders (for example: Downflow DF: lowest level is 0.30 m/s, highest level is 0.44 m/s and the actual value for downflow velocity is 0.37 m/s).

  • Page 81: Appendix Viii: Stickers

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix VIII: Stickers 1) Sticker cabinet type. This sticker can be found on the outside, underneath the cabinet. For an example of the sticker, see the picture underneath. 2) Sticker filter type. This sticker can be found behind the top hood and on top of the cabinet (for downflow filter and exhaust filter).
  • Page 82 EuroFlow EF and EF/B Biological Safety Cabinet 4) TÜV sticker 5) Work mode 6) Electro 7) Biohazard Biohazard sticker is positioned on the cabinet. The sticker has to positioned in sight of the operator. 8) Cytotoxic(only EF/B) This sticker is delivered with the cabinet. Depending on the work that is done on the cabinet the customer must decide whether there are any Cytotoxic hazards or not.
  • Page 83 EuroFlow EF and EF/B Biological Safety Cabinet 9) Cytotoxic (only EF/B) This sticker is delivered with the cabinet. Depending on the work that is done on the cabinet the customer must decide whether there are any Cytotoxic hazards or not. In case of any Cytotoxic hazards the sticker has to positioned on the cabinet above the front aperture (According to DIN 12980: 2005).

  • Page 84: Appendix Ix: Information Installed Options

    EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. Appendix IX: Information installed options Exhaust hood The exhaust hood is used in case the cabinet is connected to an external system. On the hood a thimble, a booster fan or duct can be positioned. The exhaust hood creates an extra barrier and produces extra air resistance.
  • Page 85 EuroFlow EF and EF/B Biological Safety Cabinet Screen II Installed options – relays Installed Options Relais #A1 Relais #B1 => <= Figure 47 Example of screen II Overview functions of relay K8, #A1-A12 Alarm #A1 - #A12 relay switching K8, on output J5 at the IO print: Set up from #A1 until #A12: Relay ON = A &...
  • Page 86 EuroFlow EF and EF/B Biological Safety Cabinet Screen III Analogue inputs (only if installed) Analog inputs AD1 5.0 C AD2 1.0 µM A = Enter => <= Figure 48 Example of screen III Analogue inputs can be used to program alarm settings for e.g.: temperature (°C) of working surface;...

  • Page 87: Appendix X: User Code

    EuroFlow EF & EF/B Biological Safety Cabinet Clean Air Techniek B.V. Appendix X: User code Activating the user code The function “User code Active/not active” makes it possible to lock the control of the installation. To change this state the valid user code has to be entered. The new state (active or not active) is valid after a period of 5 minutes (after touching the last button).

  • Page 88: Changing The User Code

    EuroFlow EF and EF/B Biological Safety Cabinet Changing the user code Cabinet in work mode. Cabinet in work mode - Safe §5.4 Starting up the cabinet Monday 11:06:33 on page 26. MENU Socket=Off Change the user code • Push button (Menu) and (C).

  • Page 89: Appendix Xi: Required Specifications

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix XI: Required specifications Overview of the required specifications according to EN 12469:2000 Requirement (shortened) According to: Complied: Front aperture height Between 160 and 250 [mm] EN 12469 Annex A.1 Type test: Yes Lighting At least 750 [lux] at work surface EN 12469 Annex A.2...

  • Page 90: Overview Of The Required Specifications According To Din 12980:2005

    EuroFlow EF and EF/B Biological Safety Cabinet Overview of the required specifications according to DIN 12980:2005 Requirement (shortened) According to: Complied: Lighting At least 800 [lux] DIN 12980 Chapter 11 Type test: Yes Sound level <60 [dB(A)] according to DIN 45635-1 DIN 12980 Chapter 12 Type test: Yes Vibration...

  • Page 91: Appendix Xii: Part List Critical Components

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix XII: Part list critical components Component Type Art. no. Manufacturer Type specific Downflow HEPA filter EF(/B) 4 1001216 Astrocell A99C9S2R3L HEPA 12P6+ 610x1220x69 (mm) H14 acc. EN 1822 Exhaust HEPA filter EF 4 1000404 Astrocell A99C9S2R3 HEPA 4P4 457X457X69 (mm) H14 acc.
  • Page 92 EuroFlow EF and EF/B Biological Safety Cabinet Component Type Art. no. Supplier Type specific Air velocity-sensor 1330023 Kriwan 0,2-1 m/s downflow Air velocity-sensor 1330010 Kriwan 0,2-10 m/s exhaust (=inflow) FL-holder 1440110 BJB G13 Starters 2040001 Philips PH starter S10, 220-240V, 4-65W Miniature-switch 63001P1 Omron...

  • Page 93: Appendix Xiii: Short User Manuals

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix XIII: Short user manuals Figure 49 Short user manual EF-series Figure 50 Short manual EF/B-series UM-EFAB-EN10.doc...

  • Page 94: Appendix Xiv: Tuv Low Pressure Mercury Lamps

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix XIV: TUV Low Pressure Mercury Lamps UM-EFAB-EN10.doc...

  • Page 95: Appendix Xv: Statement Of The Agreement For Machines

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix XV: Statement of the agreement for machines Manufacturer: Clean Air Techniek B.V. Address: Kuipersweg 37 3449 JA Woerden The Netherlands Phone: +31 (0) 348 411114 Fax: +31 (0) 348 422684 E-mail: info@cleanair.eu Internet: www.cleanair.eu Hereby declares that:...

  • Page 96: Appendix Xvi: Memo

    EuroFlow EF and EF/B Biological Safety Cabinet Appendix XVI: Memo UM-EFAB-EN10.doc...

  • Page 97: Index

    EuroFlow EF and EF/B Biological Safety Cabinet Index FL light, 59 —A— Aerofoil, 15 FL-lighting, 18 Alarm situations in work position, 49 Front panel, 16 Alarms, 47 Function keys, 25 Alarm-test procedure, 79 —G— Assembly-instruction, 11 Gas connection, 18 Authorized persons, 8 Gas supply off, 30 Gas supply on, 30 —B—...
  • Page 98 EuroFlow EF and EF/B Biological Safety Cabinet Replacing the Fluorescent-light, 59 Statement of the agreement for machines, 95 Stickers, 81 —S— Safety, 8 Support frame, 16 Safety symbols, 10 —T— Screen I, 42, 84 Technical specifications, 61 Screen II, 42, 85 Test aerosol, 74 Screen III, 42, 86 Testing of alarms, 45...

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