Sutter Instrument MP-285A Basic Operations
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Sutter Instrument MP-285A Basic Operations

Micromanipulator system
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MP-285
Micromanipulator System
(Including Model MP-285A Controller)
Basic Operations
Rev. 4.04 (20210122)
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax:
415-883-0572 Email: info@sutter.com
ROE

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  • Page 1 MP-285 Micromanipulator System (Including Model MP-285A Controller) Basic Operations Rev. 4.04 (20210122) One Digital Drive Novato, CA 94949 Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com...
  • Page 2 Copyright © 2021 Sutter Instrument Company. All Rights Reserved. MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 4.04 (20210122)

  • Page 5: Disclaimer

    DISCLAIMER The MP-285-system consists of one controller (MP-285 or MP-285A), a user control device (ROE (MP-285 and MP-285A) or Joystick (MP-285 only)), and one electromechanical micromanipulator device (MP-285/M). The purpose of the system is for the manipulation at the micro level of micropipettes and probes used in conjunction with a microscope. No other use is recommended.

  • Page 6: Avoiding Electrical Shock And Fire-Related Injury

    Avoiding Electrical Shock and Fire-related Injury  Always use the grounded power supply cord set provided to connect the system to a grounded outlet (3-prong). This is required to protect you from injury in the event that an electrical hazard occurs. ...

  • Page 7: Other

    Failure to do this may result in damage to the motors.  This instrument contains no user-serviceable components — do not open the instrument casing. This instrument should be serviced and repaired only by Sutter Instrument or an authorized Sutter Instrument servicing agent. ...
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  • Page 9: Table Of Contents

    TABLE OF CONTENTS DISCLAIMER ............................iii SAFETY WARNINGS AND PRECAUTIONS ................... iii Electrical ..............................iii Avoiding Electrical Shock and Fire-related Injury ................iv Electromagnetic Interference ....................... iv Operational ............................. iv Other................................. v Handling Micropipettes .......................... v 1. GENERAL INFORMATION ......................1 1.1 Structure of the MP-285 Documentation Package ................
  • Page 10 TABLE OF TABLES Table 1. MP-285 Mains fuse type and rating ..................iii Table 2. MP-285A Mains fuse type and rating ..................iii Table 3-1. Microsteps per ROE turn settings ..................12 Table 3-2. Microns per ROE turn settings ..................... 13 Table 4-1.

  • Page 11: General Information

    1.2 Technical Support Unlimited technical support is provided by Sutter Instrument Company at no charge to our customers. Our technical support staff is available between the hours of 8:00 AM and 5:00 PM (Pacific Time) at (415) 883-0128. You may also E-mail your queries to info@sutter.com.
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  • Page 13: Setup

    2.1 Unpacking Carefully remove all components from the shipping container. One of each of the following should be included:  MP-285 or MP-285A controller  MP-285 micromanipulator and D25 cable for connection to controller  Rotary Optical Encoder (ROE) and D15 cable for connection to controller ...

  • Page 14: Initial Setup And Gross Movement

    CABLE (25 pin) Figure 2-4. MP-285 controller rear connections NOTE: The MP-285A controller adds a USB port for external computer control and two BNC connectors for TTL input/output. 2. With the power OFF (front panel switch in the down position), use the D15 cable and connect the ROE to the connection labeled “COMMAND INPUT”...
  • Page 15 CAUTION: Important! Check to make sure that you have removed all six shipping screws. The unit can be severely damaged if you try to move one of the axes with the shipping screws in place. To observe movement of the manipulator, do the following: 1.

  • Page 16: Installation Of The Mp-285/M Micromanipulator On Your Microscope

    2.3 Installation of the MP-285/M Micromanipulator on your Microscope 2.3.1 General Considerations In most applications, it is best to try to mount the MP-285/M in a location that allows for the widest range of movement. Usually, this means mounting so that the device is in the center of travel when it is in its normal operating position.

  • Page 17: Mounting Headstages

    This four-hole pattern is also compatible with the MP-285 rotary base, part #285RBI and the mounting adapter plate, # 285210 (see the Reference Manual for further instructions). While it is possible to insert and tighten all four mounting screws with the MP-285 in one position, you may find it convenient to connect and power up the manipulator and position the X-axis for the best access to the screw holes.

  • Page 18: Adjusting The Rotating Dovetail Base

    Dovetail c lamp set Dovetail mounting Plastic isolation Headstag Rotating dovetail Figure 2-7. Mounting the headstage on the manipulator's Z-axis 2.5 Adjusting the Rotating Dovetail Base The rotating dovetail base allows the pipette holder or headstage to be rotated and firmly fixed at any angle desired by the experimenter as required for access to a microscope.

  • Page 19: Minimizing Electrical Noise

    2.6 Minimizing Electrical Noise We are aware of two potential noise sources for users coupling their MP-285 with high-gain, high input impedance, electrophysiological recording amplifiers. The first is associated with the manipulator and its connecting cable from the controller. Under certain circumstances these may act as an antenna concentrating electric field noise that originates from nearby electrical equipment (computer monitors, and fluorescent lights are the most notorious offenders) and bringing it into close proximity with recording apparatus.
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  • Page 21: Microscopic Manipulation

    3. MICROSCOPIC MANIPULATION 3.1 Some Basic Information Assuming you have already tried gross movement of the MP-285/M as outlined above (Initial Setup and Gross Movement) then you understand most of the features necessary for microscopic manipulation using your MP-285/M. The controller makes two changes to implement fine movement: ...

  • Page 22: Changing Minimal Resolution

    If these operations are part of your initial installation, your manipulator may not be mounted in a position to allow you to center the pipette tip in the microscope field of view. If this is true, you will have to stop and adjust the mounting location of the manipulator and/or the angle of approach of the pipette and/or the mounting of the headstage in the rotary dovetail.

  • Page 23: Table 3-2. Microns Per Roe Turn Settings

    To change resolution from one value to the other, simply press the <COARSE/FINE> button on the top of the ROE (coarse is up, fine is down). The movement screen also displays the resolution in the lower right corner “0.2 (or 0.04) µm Res”. Given a resolution in microns, we can now re-specify the amount of movement per ROE turn in microns for Setups 1 through Table 3-2.
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  • Page 25: Pulse Movement

    4. PULSE MOVEMENT So far, all the movements you have made, whether gross movement that can be easily seen by eye or precise micro moves which can only be observed with the aid of a microscope, have been in Continuous movement mode. The MP-285 controller is also capable of commanding the micromanipulator to move in pulses, where both the length and speed of the individual pulse is controlled.

  • Page 26: Table 4-1. Microsteps And Microns Per Pulse Settings

    Table 4-1. Microsteps and microns per Pulse settings Microns per Pulse at Setup Microsteps Number per Pulse 0.2µm 0.04µm 0.08 0.04 The speed of pulse movement is 5000 microsteps/second for all Setups. As there are two different resolutions, this translates to the same two different speeds for each setup: 1 µm/sec at coarse resolution, and 200 µm/sec at fine resolution.

  • Page 27: Home Function

    5. HOME FUNCTION “Go home” is the simplest of the programmable robotic functions available on the MP-285. Using the home function, you can establish a point in space to which you can command the micromanipulator to return. You may have used the home function to quickly move the manipulator to the center of travel during the “Initial Setup”...
  • Page 28  Mark your pipette puller in such a way as to make pipettes of approximately the same length.  Try to fabricate concentric pipettes.  Try to install your pipettes in the holder such that they always extend the same amount. ...

  • Page 29: Generalized Robotic Movements

    6. GENERALIZED ROBOTIC MOVEMENTS In addition to a simple movement like “Go Home”, the MP-285 is also capable of learning and remembering a complex series of Robotic movements. A series of recorded movements can be executed once or many times in a loop. The series of moves can also be executed in the reverse order in which they were recorded.

  • Page 30: Teaching The Mp-285 Controller A New Robotic Series

    have no reason to save this routine, you may wish to save it to another location simply to learn the procedure for later reference. To move program #1 to a new location and free up location #1, follow these steps (from the movement screen): 1.

  • Page 31: Executing The Newly Entered Program

    safe limits. CAUTION: Although the absolute maximum microns/sec. speed allowable in low (coarse) resolution is 6,550, it is essential that a speed no higher than 3,000 be used with the MP- 285A model controller. Now you should see a screen like the movement screen but with one exception, the <L> in the lower right corner indicates that you are in “Learn mode”.

  • Page 32: Loading A Saved Program

    3. Press <ENTR> again to accept “Do once” The screen will display the words “Executing. To stop: press key & hold until end of move”. As the robotic moves were entered at the fastest possible safe speed (3000 µm/sec) setting the move should appear significantly faster than the move to home above.

  • Page 33: Diagonal Or 4Th Axis Movements

    7. DIAGONAL OR 4TH AXIS MOVEMENTS By simultaneous movement of two axes, the MP-285 can produce movement in a synthetic fourth axis or diagonal. This feature allows for axial micropipette movement along an axis at an angle with respect to the coordinate system defined by the three axes (x, y, and z) of the manipulator.
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  • Page 35: Roe Response Characteristics

    8. ROE RESPONSE CHARACTERISTICS The response characteristics of the ROE are fully adjustable. For purposes of simplicity, this manual indicates limited adjustment to the values preprogrammed in factory installed Setups. It is possible to further tailor ROE response to your usage. The following list outlines MP-285 Reference Manual the part of the controller menu and the corresponding part of the which deals with each adjustment.
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  • Page 37: Appendix A. Limited Warranty

    Warranty work will be performed only at the factory.  The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect, after which the cost is the responsibility of the customer.
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  • Page 39: Index

    INDEX diagonal or 4th axis movements ......23 notes disclaimer ............... iii user ............... 29, 30 fuses, replacement precautions ............. iii mains ..............iii electrical .............. iii pulse movement ............ 15 general information ..........1 generalized robotic movements ......19 ROE response characteristics ......
  • Page 40 NOTES MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 4.04 (20210122)

This manual is also suitable for:

Mp-285

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