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Sutter Instrument MP-285 Basic Operations

Micromanipulator system
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Download this manual See also: Reference Manual
MP-285
Micromanipulator System
ROE
Basic Operations
Rev. 3.08 ( 20140809)
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax:
415-883-0572 Email: info@sutter.com

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Summary of Contents for Sutter Instrument MP-285

  • Page 1 MP-285 Micromanipulator System Basic Operations Rev. 3.08 ( 20140809) One Digital Drive Novato, CA 94949 Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com...
  • Page 2 Copyright © 2014 Sutter Instrument Company. All Rights Reserved. MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)

  • Page 3: Disclaimer

    SAFETY WARNINGS AND PRECAUTIONS Electrical Operate the MP-285 using 110-- 240 V AC., 50-60 Hz line voltage. This instrument is designed for use in a laboratory environment that has low electrical noise and mechanical vibration. Surge suppression is recommended at all times.

  • Page 4: Electromagnetic Interference

    ROE, MP-285/M electromechanical device, and host computer via serial RS-232 interface, (see the Technical Specifications appendix for more details).

  • Page 5: Handling Micropipettes

    Failure to do this may result in damage to the motors. This instrument contains no user-serviceable components — do not open the instrument casing. This instrument should be serviced and repaired only by Sutter Instrument or an authorized Sutter Instrument servicing agent.
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  • Page 7: Table Of Contents

    1.1 Structure of the MP-285 Documentation Package ................1 1.2 Technical Support..........................1 2. SETUP...............................3 2.1 Unpacking............................3 2.2 Initial Setup and Gross Movement....................4 2.3 Installation of the MP-285 Micromanipulator on your Microscope..........6 2.3.1 General Considerations ......................6 2.3.2 Specifics ............................6 2.4 Mounting Headstages........................7 2.5 Adjusting the Rotating Dovetail Base.....................8 2.6 Minimizing Electrical Noise ......................9...
  • Page 8 Figure 2-8. Adjusting the headstage angle....................8 TABLE OF TABLES Table 3-1. Microsteps per ROE turn settings..................12 Table 3-2. Microns per ROE turn settings.....................13 Table 4-1. Microsteps and microns per Pulse settings.................16 MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)

  • Page 9: General Information

    The first part, “Basic Operations”, has been kept short and covers major functions with the aim of quickly guiding the first time user through the setup and use of the MP-285 for micromanipulation with emphasis on using the ROE (Rotary Optical Encoder) for user control.
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  • Page 11: Setup

    Carefully remove all components from the shipping container. One of each of the following should be included: MP-285 controller MP-285 micromanipulator and D25 cable for connection to controller Rotary Optical Encoder (ROE) and D15 cable for connection to controller Controller power cord...

  • Page 12: Initial Setup And Gross Movement

    1. Connect the power cord to the MP-285 controller. Make certain that the voltage selector next to the power cord socket on the back of the MP-285 controller has been set to the appropriate voltage (110 or 220).
  • Page 13 . w i t h * 2. Press the <MOVE> key on the MP-285 controller keypad. Observe the movement screen: - 1 0 7 8 9 . 2 0 - 1 0 7 7 8 .

  • Page 14: Installation Of The Mp-285 Micromanipulator On Your Microscope

    2.3 Installation of the MP-285 Micromanipulator on your Microscope 2.3.1 General Considerations In most applications, it is best to try to mount the MP-285 in a location that allows for the widest range of movement. Usually, this means mounting so that the device is in the center of travel when it is in its normal operating position.

  • Page 15: Mounting Headstages

    # 285210 (see the Reference Manual for further instructions). While it is possible to insert and tighten all four mounting screws with the MP-285 in one position, you may find it convenient to connect and power up the manipulator and position the X-axis for the best access to the screw holes.

  • Page 16: Adjusting The Rotating Dovetail Base

    It is not recommended that you use this rotational joint as a means of pipette replacement. Repeated repositioning of the rotary disk and brake will lead to rapid deterioration of the joint which may then not tighten properly to allow stable positioning of a pipette. MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)

  • Page 17: Minimizing Electrical Noise

    2.6 Minimizing Electrical Noise We are aware of two potential noise sources for users coupling their MP-285 with high-gain, high input impedance, electrophysiological recording amplifiers. The first is associated with the manipulator and its connecting cable from the controller. Under certain circumstances...
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  • Page 19: Microscopic Manipulation

    Reference Manual. 3.2 SETUP Selection In order for the MP-285 to make precise movements and have a greater amount of control over the movement, you instruct the controller to produce fewer microsteps (the minimal increment of manipulator movement) per rotation of the ROE knobs.

  • Page 20: Changing Minimal Resolution

    3. Press <MOVE> to return to the movement screen and begin moving at the precision specified by the selected Setup. The selected setup is maintained when the MP-285 controller is turned off; you only need to select a different setup when you wish to change precision of movement.

  • Page 21: Table 3-2. Microns Per Roe Turn Settings

    (#4, fine) for micromanipulation under the microscope. You may find that none of these SETUPS delivers the type of responsiveness that you desire from the MP-285. The MP-285 Reference Manual provides complete instructions for defining and saving your own Setup.
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  • Page 23: Pulse Movement

    Continuous movement mode. The MP-285 controller is also capable of commanding the micromanipulator to move in pulses, where both the length and speed of the individual pulse is controlled.

  • Page 24: Table 4-1. Microsteps And Microns Per Pulse Settings

    You may find that no SETUP delivers the size and or speed pulses that you desire. The reference manual gives complete instructions for defining pulse size and speed and saving defined values to a new setup. MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)

  • Page 25: Home Function

    5. HOME FUNCTION “Go home” is the simplest of the programmable robotic functions available on the MP-285. Using the home function, you can establish a point in space to which you can command the micromanipulator to return. You may have used the home function to quickly move the manipulator to the center of travel during the “Initial Setup”...
  • Page 26 Details on how to specify speed and resolution are given in the MP-285 Reference Manual. MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)

  • Page 27: Generalized Robotic Movements

    6. GENERALIZED ROBOTIC MOVEMENTS In addition to a simple movement like “Go Home”, the MP-285 is also capable of learning and remembering a complex series of Robotic movements. A series of recorded movements can be executed once or many times in a loop. The series of moves can also be executed in the reverse order in which they were recorded.

  • Page 28: Teaching The Mp-285 Controller A New Robotic Series

    Note that the original copy of program #1 is still in location 1 and will be overwritten when new robotic moves are entered. 6.4 Teaching the MP-285 Controller a New Robotic Series You are ready to teach the controller a series of movements to robotically move the manipulator to a location where pipette exchange is possible: 1.

  • Page 29: Executing The Newly Entered Program

    The screen will display the words “Executing. To stop: press key & hold until end of move”. As the robotic moves were entered at the fastest possible speed (6550mm/sec) setting the move should appear significantly faster than the move to home above. If you wish, you can MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)

  • Page 30: Loading A Saved Program

    2. <TAB> 5X to “Select program” and press <ENTR> 3. <TAB> to “Load” and press <ENTR> to accept 4. <TAB> to a desired program and press <ENTR> 5. <ESC> back to the movement screen MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)

  • Page 31: Diagonal Or 4Th Axis Movements

    7. DIAGONAL OR 4TH AXIS MOVEMENTS By simultaneous movement of two axes, the MP-285 is able to produce movement in a synthetic fourth axis or diagonal. This feature allows for axial micropipette movement along an axis at an angle with respect to the coordinate system defined by the three axes (x, y, and z) of the manipulator.
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  • Page 33: Roe Response Characteristics

    Controller Configuration and is adjusted using the menu command [PRGM\Setup\Axes]. 8.2 Controlling Movement Amount per ROE Turn How much and how fast the MP-285 manipulator moves in response to a single turn of the ROE knob is controlled by a single parameter for continuous movement and two parameters for pulse movement.
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  • Page 35: Appendix A. Limited Warranty

    Warranty work will be performed only at the factory. The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect, after which the cost is the responsibility of the customer.
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  • Page 37: Index

    & precautions operational............iv mains safety warnings & precautions......iii, iv fuses ..............iii setup................3 microscopic manipulation........11 movement amount per ROE turn........25 technical support .............1 control of.............25 warranty ..............27 NOTES MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
  • Page 38 NOTES MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)

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