Invitrogen Safe Imager G6600 Quick Reference Card

Safe Imager 2.0 Blue-Light Transilluminator
™
Catalog nos. G6600, G6600EU, G6600UK
Introduction
The Safe Imager 2.0 Blue-Light Transilluminator (Fig. 1, A) is
™
designed for viewing stained gels on the laboratory bench top, or
within a gel documentation system. The Safe Imager
plied with a Safe Imager amber filter unit (Fig. 1, B), a power
™
cord (Fig. 1, C), and Safe Imager
Light from the LED source inside the transilluminator passes
through a blue filter producing a single-intensity signal at
approximately 470 nm (Fig. 2), effective for the excitation of
SYBR ® Safe DNA gel stain, and many other other Invitrogen pro-
prietary nucleic acid and protein stains, such as SYBR
®
SYBR Green I and II, SYPRO
Coomassie Fluor Orange stains. Sensitivity obtained using this
™
instrument is comparable to that obtained with a standard UV
transilluminator.
Unlike UV-transilluminators, the Safe Imager
Transilluminator does not produce UV light and does not require
UV-protective equipment during use.
Figure 2. Emission spectrum for the Safe Imager
Transilluminator.
Operating the Safe Imager
Transilluminator
Important: Before handling your gel or sample, ensure that the
personal safety equipment you are using is appropriate for the
hazards posed by the chemicals that may be present. Make sure
to use either the Safe Imager
™
viewing glasses; to prevent prolonged exposure of your eyes to
the intense blue light (see Safety Information for more details).
1. Ensure that the Safe Imager
is placed on a level bench and that there is adequate air
circulation around the unit to prevent overheating.
2. Attach the supplied power cord to the Safe Imager
light transilluminator at the back of the device. Plug the other
end of the power cord into a properly grounded electrical out-
let, ensuring the correct plug adaptor is attached (see Safety
Information for additional details).
Note: The small footprint of the Safe Imager
transilluminator may allow it to fit inside the cabinet of your
current gel documentation system.An additional adapter is
supplied to allow direct connection of the power supply unit
to a C14 type cord found in some imaging systems.
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2.0 is sup-
™
viewing glasses (Fig. 1, D).
™
®
Gold,
® ®
Ruby, SYPRO Orange, and
2.0 Blue-Light
™
™ 2.0 Blue-Light
™
2.0 Blue-Light
amber filter unit or Safe Imager
2.0 Blue-Light Transilluminator
™
2.0 blue-
™
2.0 blue-light
™
25-1054 Rev. Date: 28 August 2009
B
Figure 1. The Safe Imager
3. Place the gel or sample onto the surface of the Safe Imager
2.0 Blue-Light Transilluminator.
4. Place the amber filter unit on top of the sample or stained gel.
If the gel is larger than the viewing area, rest the amber filter
unit directly on top of the gel, or forgo the amber filter unit
and rely solely on the viewing glasses.
5. Switch the Safe Imager
ON/OFF switch located at the front of the instrument (Fig. 3).
DNA stained with blue light-excitable dyes (in solution or in
gel bands) are immediately visible after illumination and the
amber filter unit or viewing glasses are in position.
Figure 3. On / Off switch: Red mark is seen when on.
6. Any standard imaging device may be used to document
results. Satisfactory results are often obtained by placing the
™
amber filter unit on top of the gel and photographing/
imaging as normal.
Note: The Safe Imager
very slim design compared to a UV transilluminator, so the
distance between the camera and gel may require adjustment.
Also, some CCD documentation systems may include a filter
that will work in place of the amber filter unit (contact the
manufacturer for filter specifications).
7. Use the viewing glasses when excising bands from DNA gels.
The viewing glasses allow the bands to be visualized while
leaving the gel surface unobstructed.
Note: Blue-light transillumination is ideal for cloning, and
results in dramatically increased cloning efficiencies compared
to UV transillumination (Fig. 4).
8. Switch the Safe Imager
after use.
C
A
™ 2.0 Blue-Light Transilluminator.
2.0 transilluminator ON using the
™
2.0 Blue-Light Transilluminator has a
™
2.0 Blue-Light Transilluminator off
™
Contact Information for Other Countries:
See our website www.invitrogen.com
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