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Summary of Contents for Invitrogen Bandmate BW1000
- Page 1 ™ Bandmate Automated Western Blot Processor USER GUIDE For automation of western blot processing Catalog Numbers BW1000 Publication Number MAN0018722 Revision C.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Thermo Fisher Scientific | 3747 N. Meridian Road | Rockford, Illinois 61101 USA For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
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Page 3: Table Of Contents
Contents ■ ™ CHAPTER 1 Bandmate Automated Western Processor ..... . . 4 Product description ............. 4 Contents . -
Page 4: Chapter 1 Bandmate
Processor Product description ™ ™ The Invitrogen Bandmate Automated Western Blot Processor is a self-contained western blot processing system that can process up to 4 mini blots or 2 midi blots at a time. Each blot can be probed with different antibodies, if desired. The instrument has built-in traditional programs, but permits custom programs for incubation and wash times. -
Page 5: Contents
Chapter 1 Bandmate ™ Automated Western Processor Contents Table 1 Program icons Icon Function Icon Function Accept Fill Back Home Cancel (Stop) Pause Continue Drain Save Edit Contents ™ Table 2 Bandmate Automated Western Blot Processor (Cat. No. BW1000) Contents Amount Buffer Hose w/ Quick-connect Coupling Interior Siphon Hose for Buffer Bottle... -
Page 6: Operate The Bandmate ™ Automated Western Blot Processor
Chapter 1 Bandmate ™ Automated Western Processor ™ Operate the Bandmate Automated Western Blot Processor Table 3 Available for separate purchase (continued) Product Cat. No. Waste Tray BW0030 Funnel Tray BW0040 Tray Cover BW0050 4 L Buffer Bottle BW0010 Table 4 Accessory products Product Cat. -
Page 7: Program The Processor
Chapter 1 Bandmate ™ Automated Western Processor ™ Operate the Bandmate Automated Western Blot Processor Program the processor • Store up to 8 programs. • All incubation steps can be set from 1 second to 96 hours. • Volume range for washes is between 10 mL to 75 mL for mini gels and 10 mL to 150 mL for midi gels. - Page 8 Chapter 1 Bandmate ™ Automated Western Processor ™ Operate the Bandmate Automated Western Blot Processor 5. On the last screen, input the times and reagent for each step in your protocol. Buffer wash steps can be repeated up to ten times, but reagent steps cannot be repeated. When selecting the step, the following may be chosen: Buffer;...
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Page 9: Run The Processor
Chapter 1 Bandmate ™ Automated Western Processor ™ Operate the Bandmate Automated Western Blot Processor Note: When modifying an existing program, it is important to save before starting the program. Only saved in-process programs will automatically resume after a power shutdown or outage. Run the processor 1. -
Page 10: Clean The Processor
Chapter 1 Bandmate ™ Automated Western Processor ™ Operate the Bandmate Automated Western Blot Processor 9. To recover the antibody solutions, place empty 50 mL uncapped conical tubes in the appropriate spaces on each side of the waste tray (space #1 for the primary antibody and #2 for the secondary antibodies). - Page 11 Chapter 1 Bandmate ™ Automated Western Processor ™ Operate the Bandmate Automated Western Blot Processor Calibration The pumps that deliver wash buffer to the tray have been calibrated at the factory. To check or re-calibrate the pumps, use the Calibration function. Pumps should be re-calibrated every 6 months or when moving the instrument from room temperature to a cold room or vice versa.
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Page 12: Instrument Dimensions And Specifications
Chapter 1 Bandmate ™ Automated Western Processor Troubleshooting General system cleaning • Rinse the waste tank and funnel tray. The trays are resistant to chemicals typically used in a western blot, but some of the components of blocking buffers (nonfat dry milk, BSA, etc.) can form deposits on the surface. - Page 13 Chapter 1 Bandmate ™ Automated Western Processor Troubleshooting Observation Possible cause Recommended action Processor stopped working. Processor electronics errored. Turn the system off for one minute to allow the electronics to reset. If the processor does not turn on after resetting, contact technical support.
- Page 14 Chapter 1 Bandmate ™ Automated Western Processor Troubleshooting Observation Possible cause Recommended action There is no detection for The primary antibody might Perform a short program with only the targets that were successfully have been placed on position secondary antibody to see if the band will detected using traditional number 2 instead of position appear.
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Page 15: Appendix A Safety
Safety WARNING! GENERAL SAFETY. Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document. -
Page 16: Control And Connection Symbols
Appendix A Safety Symbols on this instrument Control and connection symbols Symbols and descriptions On (Power) Off (Power) Earth (ground) terminal Protective conductor terminal (main ground) Direct current Alternating current Both direct and alternating current Conformity symbols Conformity mark Description Indicates conformity with safety requirements for Canada and U.S.A. -
Page 17: Safety Information For Instruments Not Manufactured By Thermo Fisher Scientific
Appendix A Safety Safety information for instruments not manufactured by Thermo Fisher Scientific Safety information for instruments not manufactured by Thermo Fisher Scientific Some of the accessories provided as part of the instrument system are not designed or built by Thermo Fisher Scientific. -
Page 18: Physical Injury
Appendix A Safety Instrument safety Physical injury CAUTION! Moving and Lifting Injury. The instrument is to be moved and positioned only by the personnel or vendor specified in the applicable site preparation guide. Improper lifting can cause painful and permanent back injury. Things to consider before lifting or moving the instrument or accessories: ·... -
Page 19: Cleaning And Decontamination
Appendix A Safety Safety and electromagnetic compatibility (EMC) standards Cleaning and decontamination CAUTION! Cleaning and Decontamination. Use only the cleaning and decontamination methods that are specified in the manufacturer user documentation. It is the responsibility of the operator (or other responsible person) to ensure that the following requirements are met: ·... -
Page 20: Emc Standards
Appendix A Safety Safety and electromagnetic compatibility (EMC) standards EMC standards Reference Description EU Directive 2014/30/EU European Union “EMC Directive” EN 61326-1 Electrical Equipment for Measurement, Control and Laboratory Use – EMC Requirements – Part 1: General Requirements IEC 61326-1 AS/NZS CISPR 11 Limits and Methods of Measurement of Electromagnetic Disturbance Characteristics of Industrial, Scientific, and Medical (ISM) Radiofrequency Equipment... -
Page 21: Environmental Design Standards
Appendix A Safety Safety and electromagnetic compatibility (EMC) standards Environmental design standards Reference Description Directive 2012/19/EU European Union “WEEE Directive”—Waste electrical and electronic equipment Directive 2011/65/EU European Union “RoHS Directive”—Restriction of hazardous substances in electrical and electronic equipment SJ/T 11364-2014 “China RoHS”... -
Page 22: Chemical Safety
Appendix A Safety Chemical safety Chemical safety WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below. Consult the relevant SDS for specific precautions and instructions: ·... -
Page 23: Biological Hazard Safety
Appendix A Safety Biological hazard safety Biological hazard safety WARNING! Potential Biohazard. Depending on the samples used on this instrument, the surface may be considered a biohazard. Use appropriate decontamination methods when working with biohazards. WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. -
Page 24: Appendix B Documentation And Support
Documentation and support Customer and technical support Visit thermofisher.com/support for the latest service and support information. • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments •... - Page 26 Bandmate Automated Western Processor_UG_MAN0018722-v6- GUID-8DBD70A8-7BC4-4AB0-919A-5601AB0A9781-2021/07/19 19:26:41 en 15:09:51.386+01:00 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 14 September 2021...
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