Invitrogen A25977 Quick Reference

Mini Gel Tank
Cat. no. A25977
Publication Part No. 100025990
Instructions for using the Mini Gel Tank to perform electrophoresis are described below. For detailed instructions, refer to the manual
available from thermofisher.com. See reverse side for protein transfer instructions using the Mini Blot Module.
Before Starting
• Make sure that the power supply is adequate for the number of gels you are going to run.
• If your power supply is not designed for use with covered or retractable power leads, make sure the power supply is off, plug Novex
Power Supply Adapters into the appropriate leads of the power supply, and secure them with an Allen wrench (see manual for details).
• Select mini gels appropriate for your application.
• Prepare 1X running buffer appropriate for your mini gels. Each chamber requires 400 mL of buffer.

Procedure

1
(+) Anode connectors
Cathode
1.
Snap the electrophoresis tank into the
base, and place the cassette clamp(s)
into the chamber(s) with the anode
connector(s) (+) aligned to the center.
Fill the chamber(s) with 1X buffer to the
level of the cathode.
4
4.
Make sure the wells are completely
filled with 1X buffer.
Load your samples and markers.
For Research Use Only. Not for use in diagnostic procedures.
Publication No. MAN0010862
2
Cathode
2.
Remove the comb, and peel away the
tape at the bottom of the gel cassette.
Rinse the wells 3 times with 1X buffer.
5
5.
Hold the cassette and release the
cassette clamp.
Gently lower the cassette so that it rests
on the bottom of the chamber, and close
the cassette clamp.
Add 1X buffer to the level of the fill line.
Rev. C.0
3
3.
6
6.
QUICK REFERENCE
Cam
Handle
Place the cassette in the chamber with
the wells facing towards you.
Hold the cassette in a raised position
and close the clamp by moving the cam
handle forward.
Make sure the power supply is off.
If only running one gel, remove the
cassette clamp from unused chamber.
Place the lid on the tank and plug the
electrode cords into the power supply.
Turn the power supply on to begin
electrophoresis.
™