Advertisement
M2551 Mag-Bind® FFPE RNA 96 Kit Protocol
Mag-Bind® FFPE RNA 96 Kit Protocol - 96-well plates
Materials and Equipment to be Supplied by User:
•
Nuclease-free 1.2 mL round-well plates
•
Nuclease-free microplates
•
Centrifuge with swing-bucket rotor capable of 4,000 x g
•
RNase-free filter pipette tips
•
Magnetic separation device for 96-well plates (MSD-01B or MSD-01)
•
Water bath or heat block capable of 55°C
•
Water bath or heat block capable of 80°C
•
Water bath or heat block capable of 37°C
•
100% ethanol
•
Sealing film
Before Starting:
•
Prepare RNA Wash Buffer II and GFC Buffer according to the Preparing Reagents
section on Page 4
•
Set water bath or heat block to 55°C
•
Set water bath or heat block to 80°C
•
Set water bath or heat block to 37°C
1.
Add 250 μL RML Buffer into each well of a 1.2 mL round-well plate.
2.
Cut 2-5 paraffin sample sections between 5-10 μm to be placed in each well of the 96
plate. Note: Do not use the first 2-3 sections.
3.
Immediately place 2-5 sections into each well of the round-well plate.
4.
Centrifuge at 4,000 x g at room temperature for 2 minutes.
5.
Incubate at 80°C for 15 minutes to melt the paraffin. Mix the sample a few times by
gently shaking the tube. Make sure that the tissue sections stay submerged in the
solution.
Note: Seal the plate with sealing film to prevent evaporation during incubation.
6
Advertisement
