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M2551 Mag-Bind® FFPE RNA 96 Kit Protocol
16. Place the plate onto the magnet separation device to magnetize the Mag-Bind®
Particles SC. Wait 3-5 minutes or until all the Mag-Bind® Particles SC are cleared from
solution.
17. Aspirate and discard the cleared supernatant. Remove any liquid drops from each
well.
18. Add 73.5 µL DNase I Digestion Buffer and 1.5 µL RNase-free Mag-Bind® DNase I.
Resuspend the Mag-Bind® Particles SC thoroughly by vortexing for 20 seconds or
pipetting up and down 10-20 times.
19. Incubate at 37°C for 15 minutes.
20. Add 225 µL GFC Buffer. Mix thoroughly by vortexing for 20 seconds or pipetting up
and down 10-20 times.
Note: GFC Buffer must be diluted with 100% ethanol prior to use. Please see
instructions on Page 4.
21. Let sit at room temperature for 3-5 minutes.
22. Place the plate onto a magnet separation device to magnetize the Mag-Bind®
Particles SC. Wait 3-5 minutes or until all the Mag-Bind® Particles SC are cleared from
solution.
23. Aspirate and discard the cleared supernatant.
24. Remove the plate from the magnetic separation device.
25. Add 400 µL RNA Wash Buffer II. Resuspend the Mag-Bind® Particles SC thoroughly by
vortexing for 20 seconds or pipetting up and down 10-20 times.
26. Place the plate onto a magnet separation device to magnetize the Mag-Bind®
Particles SC. Wait 3-5 minutes or until all the Mag-Bind® Particles SC are cleared from
solution.
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