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Mag-Bind® FFPE RNA 96 Protocol with Xylene
27. Incubate at 37°C for 15 minutes.
28. Add 225 µL GFC Buffer. Mix thoroughly by vortexing for 20 seconds or pipetting up
and down 10-20 times.
Note: GFC Buffer must be diluted with 100% ethanol prior to use. Please see
instructions on Page 4.
29. Let sit at room temperature for 3-5 minutes.
30. Place the plate onto a magnet separation device to magnetize the Mag-Bind®
Particles SC. Wait 3-5 minutes or until all the Mag-Bind® Particles SC are cleared from
solution.
31. Aspirate and discard the cleared supernatant.
32. Remove the plate from the magnetic separation device.
33. Add 400 µL RNA Wash Buffer II. Resuspend the Mag-Bind® Particles SC thoroughly by
vortexing for 20 seconds or pipetting up and down 10-20 times.
34. Place the plate onto a magnet separation device to magnetize the Mag-Bind®
Particles SC. Wait 3-5 minutes or until all the Mag-Bind® Particles SC are cleared from
solution.
35. Aspirate and discard the cleared supernatant. Remove any liquid drops from the
wells.
36. Repeat Step 32-35 for a second RNA Wash Buffer II wash step.
37. Air dry the Mag-Bind® Particles SC by leaving the plate on the magnetic separation
device for 10 minutes. Remove any residual liquid with a pipette.
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