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M2551 Mag-Bind® FFPE RNA 96 Kit Protocol
27. Aspirate and discard the cleared supernatant. Remove any liquid drops from tube.
28. Repeat Step 24-27 for a second RNA Wash Buffer II wash step.
29. Air dry the Mag-Bind® Particles SC by leaving the plate on the magnetic separation
device for 10 minutes. Remove any residual liquid with a pipette.
30. Add 30-50 µL DEPC Water. Resuspend the Mag-Bind® Particles SC thoroughly by
vortexing for 30 seconds or pipetting up and down 30 times.
31. Let sit at room temperature for 10 minutes.
32. Place the plate onto a magnet separation device to magnetize the Mag-Bind®
Particles SC. Wait 3-5 minutes or until all the Mag-Bind® Particles SC are cleared from
solution.
33. Transfer the cleared supernatant containing purified RNA into a new nuclease-free
96-well microplate (not supplied) and seal with sealing film.
34. Store the purified RNA at -80°C.
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